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Citations for Bio-Rad :
4551 - 4600 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: Analytical size exclusion chromatography was carried out on a Waters HPLC with an ENrichTM SEC 650 10 x 300 column (Bio-Rad) in 20 mM HEPES pH 8.0 ...
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bioRxiv - Biophysics 2023Quote: ... UBQLN1 D15R and UBQLN1 E21Q were subjected to size exclusion chromatography over an ENrich™ SEC 650 10 x 300 column (BioRad) in 20 mM sodium phosphate buffer pH 6.8 ...
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bioRxiv - Biophysics 2023Quote: ... samples were processed using a Bio-Rad NGC Quest 10 Plus fast protein liquid chromatography (FPLC) apparatus with a 5 mL EconoFit Nuvia IMAC affinity column (Bio-Rad). To purify protein from the soluble fraction ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were denatured by 5 min incubation at 95°C before running them on a TGX 4–12% or 10% gel (Bio-Rad) and transferred onto nitrocellulose membrane for hybridization with specific antibodies.
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bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by sodium-dodecyl-sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using Mini TGX gels (10%, Bio-Rad, Hercules, USA) according to standard protocols and transferred to nitrocellulose membranes (0.2 μm ...
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bioRxiv - Neuroscience 2024Quote: ... Gene expression was analyzed by qPCR in 20ng of cDNA in triplicate 10 µl reactions using SYBR Green Taq MasterMix (Bio-Rad), following the manufacturer’s instructions in a CFX-384 Real-Time PCR thermocycler (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... cDNA was diluted 1/5 and quantified by RT-qPCR in 10 μL final reactions using 2X iTaq Universal Sybr Green Supermix (#1725125, Bio-Rad) in CFX96 Touch Real-Time PCR machines (Bio-Rad) ...
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Pushed to the edge: hundreds of myosin 10s pack into filopodia and could cause traffic jams on actinbioRxiv - Cell Biology 2024Quote: ... and heated at 70°C for 10 min before loading onto a 4-20% Mini-PROTEAN TGX Stain-Free protein gel (BioRad, 4568095). The gel was run at 180 V for 45 min and imaged on a ChemiDoc ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing protein were pooled and desalted using the Econo-Pac® 10-DG Desalting Columns (Bio-Rad, Cat#732-2010). To this end ...
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bioRxiv - Neuroscience 2024Quote: ... 1:5000 dilution in 10% milk) the membranes were imaged as described above but using Clarity Max ECL Western Blotting Substrate (BioRad, 1705062).
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bioRxiv - Cancer Biology 2024Quote: ... 10 μl of the cell suspension was used to estimate the number of cells by an automated cell counter (BioRad TC20). Based on this ...
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bioRxiv - Cancer Biology 2024Quote: ... submerged in blocking buffer (40% ethanol and 10% acetic acid in distilled water) with shaking for 15 min and stained with coomassie blue dye (Bio-Rad) by gentle shaking overnight ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were boiled for 5-10 minutes and then separated using SDS-PAGE with 4-12% Criterion XT Bis-Tris Gels (Bio-Rad). The proteins were transferred to PVDF membranes (Merck Millipore) ...
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bioRxiv - Biochemistry 2024Quote: ... using gels with a concentration of 8%, 10%, 14% or 15% acrylamide (Carl Roth, 3029.1) in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell system (Bio-Rad, 1658029FC) with running buffer containing 0.2 M glycine (Sigma-Aldrich ...
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bioRxiv - Immunology 2024Quote: ... 15μg of protein was loaded for each sample in precast 4-15% Mini-Protean TGX Stain-Free 30μl 10-well polyacrylamide gels (Bio-Rad #456803) along with Kaleidoscop protein ladder (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... heated at 95°C for 10 min and run on a Mini-protean TGX 4%-20% polyacrylamide gel (Biorad, 456-1095). The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... boiled for 10 min at 95°C and examined by western blot using 4-15% Mini-PROTEAN TGX precast protein gels (BioRad #4561083). After blocking step with 10% non-fat milk for 1h at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were washed 3 times for 10 min with TBS-T and developed with an enhanced chemiluminescence (ECL Bio-Rad 1705061) substrate ...
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bioRxiv - Cell Biology 2024Quote: The protein extracts were separated by 10% SDS-PAGE and the proteins were transferred on the low fluorescence PVDF membrane (Bio-rad). The molecular weight of the proteins was assigned by Precision Plus Protein Dual Color Standards (Bio-Rad) ...
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bioRxiv - Microbiology 2024Quote: ... The gels were subsequently transferred for 10 min onto a polyvinylidene difluoride membrane (PVDF) using the Trans-Blot turbo transfer system (Bio-rad). The membranes were blocked using blocking buffer prepared using 5% milk (Alkali Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 250-400 µL of the clarified sample was combined with 10-30 µL of a molecular weight marker solution prepared from Gel Filtration Standard (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2024Quote: ... the samples were combined with SureBeads™ Protein G (10 μL in 50% incubation buffer slurry; Bio-Rad, Hercules, CA, USA). After 3 h of incubation at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... and 10 μL of whole tissue lysate preps) were applied to 4−15% SDS-polyacrylamide gels (Bio-Rad; Hercules, CA, USA) and subjected to electrophoresis at 180 volts for 50 minutes in a preformulated 1× SDS-PAGE buffer (VWR) ...
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bioRxiv - Physiology 2024Quote: ... The libraries were diluted to 10 nM and further quantitated by qPCR on a CFX Connect Real-Time qPCR system (Bio-Rad) for accurate pooling of barcoded libraries and maximization of number of clusters in the flowcell.
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bioRxiv - Molecular Biology 2021Quote: ... PCR products were resolved on 1× TAE 1% agarose gels and imaged using Gel Doc EZ System (Bio-Rad). Raw gel scans are provided in SI Data File 4 ...
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bioRxiv - Cell Biology 2021Quote: ... HRP (horseradish peroxidase)-conjugated goat anti-mouse (#176516, 1:10,000 for WB) and anti-rabbit IgG antibodies (#176515, 1:10,000 for WB) were from Bio-Rad. Alexa Fluor conjugated goat anti-mouse (1:500 for IF) ...
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bioRxiv - Biochemistry 2022Quote: ... the responses obtained from the AmQNS-small molecular interactions at different concentrations were fitted using the Langmuir 1:1 biomolecular interaction model using the ProteOn Manager software version 3.1.0.6 (Bio-Rad, USA). Equilibrium dissociation constants (KD ...
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bioRxiv - Microbiology 2020Quote: One ug of each respective protein was mixed at a 1:1 ratio with 2X Laemmli buffer (Bio-Rad) which was supplemented with 2% β-mercaptoethanol (Fisher Scientific) ...
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bioRxiv - Physiology 2021Quote: ... Lysates were centrifuged to pellet tissue debris and equal volumes mixed 1:1 with 2x Laemmli sample buffer (BioRad). Samples were boiled ...
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bioRxiv - Biochemistry 2021Quote: ... was performed with 1:1,000 or 1:100,000 diluted PCR1 product using SsoAdvanced Universal SYBR green supermix (Bio-Rad). To quantify the Pten 5-6k isoform ...
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bioRxiv - Genetics 2019Quote: ... at 4° C with 100 V for 1 hour in 1 x Tris/Glycine Buffer (Bio-Rad, Cat#1610734) and 20% methanol (Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... GroEL was detected with anti-GroEL primary antibody (Pierce; 1:10,000) and anti-rabbit secondary antibody (Biorad; 1:20,000).
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bioRxiv - Biochemistry 2024Quote: ... Samples were loaded onto polyacrylamide native gels (5% polyacrylamide 29:1 [BioRad], 1× TBE, 0.3% APS, and 0.06% Temed) and protein/DNA complexes were resolved by electrophoresis at 60 V for 2 h at 24 °C in 1X TBE ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was washed four times in TBS-T for 5 min each before incubation for 1 h with secondary antibody (1:2,000 dilution of BioRad affinity purified goat α-rabbit IgG conjugated to alkaline phosphatase ...
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bioRxiv - Neuroscience 2023Quote: PFA fixed brain samples were incubated in 1% hydrogel monomers (HMs) consisting of 1% w/v acrylamide (#1610140, BioRad), 0.05% w/v bisacrylamide (#1610142 ...
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bioRxiv - Biochemistry 2023Quote: ... The nitrocellulose membrane was exposed to a 1:1 v/v solution of Clarity Western ECL Substrate (Bio-rad) for 5 mins and chemiluminescence quantified and analysed using FluorChem E software (ProteinSimple).
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bioRxiv - Neuroscience 2023Quote: ... The membranes were washed three times with TBST and then incubated for 60 min at room temperature with horseradish peroxidase-conjugated secondary antibodies (1:3,000 dilution, ref: 172-1011 and 1:5,000 dilution, ref: 170-6515, Bio-Rad) in blocking buffer ...
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bioRxiv - Plant Biology 2020Quote: ... Complementary DNA (cDNA) was synthesized using the iScript cDNA synthesis kit (Bio-Rad) with primers (Supporting Information Table S1 ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was synthesized by reverse transcription using the iScript kit (BioRad, Stockholm, Sweden). Transcripts of interest were analyzed using the QuantStudio 5 real-time quantitative PCR (RT-qPCR ...
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bioRxiv - Cell Biology 2020Quote: ... cDNA was generated using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories Inc.). qRT-PCR was performed using Power SYBR Green master mix (Applied Biosystems ...
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed with the iScript cDNA Synthesis kit (Biorad, Munich, Germany) and cDNA was analyzed by qPCR using the SsoFast Eva Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... First strand cDNA was made using the iScript cDNA Synthesis Kit (Bio-Rad). CT values from quantitative PCR were analyzed with the delta delta CT method using 18S as an internal control (Livak and Schmittgen ...
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bioRxiv - Cell Biology 2020Quote: ... Total protein amount was determined (DC Protein Assay kit, Bio-Rad, Hercules, CA) and samples equalized with ddH2O and NuPAGE LDS 4x Sample Buffer (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... Immunoreactivity was revealed using the Clarity™ ECL Western Blotting Substrate kit (BioRad) and images were obtained using a BioRad ChemiDoc MP system ...
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bioRxiv - Developmental Biology 2021Quote: ... and cDNA synthesis was performed using the iScript cDNA Synthesis Kit (Bio-Rad). qRT-PCR was performed with a SYBR Green PCR Master Mix and the 7900 Real time PCR system (Applied Biosystems) ...
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bioRxiv - Developmental Biology 2020Quote: ... Complementary DNA was generated using the iScript cDNA synthesis kit (Bio-Rad Laboratories) from 10 ng total RNA ...
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bioRxiv - Developmental Biology 2020Quote: ... Protein was quantified using the DC™-protein assay kit (Bio-Rad, 5000112) using bovine serum albumin as standard ...
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bioRxiv - Developmental Biology 2022Quote: ... We carried out cDNA synthesis using the iScript cDNA Synthesis Kit (Bio-Rad) as instructed ...
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bioRxiv - Neuroscience 2021Quote: ... cDNA was synthesized using the iScript cDNA Synthesis Kit (Bio-Rad, Philadelphia, PA). qPCR was performed using two embryo-equivalents for each reaction using Power SYBR Green PCR Master Mix (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was then reverse transcribed using the iScript cDNA synthesis kit (Bio-Rad) as per the manufacturer’s instructions ...