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Citations for Bio-Rad :
4351 - 4400 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... A volume of cDNA corresponding to 5 ng of the input RNA was subjected to quantitative PCR (qPCR) in a 10 μl reaction using the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad) and 0.4 μM of each forward and reverse primer ...
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bioRxiv - Plant Biology 2022Quote: ... The extract was centrifuged at 18,000 g for 10 minutes at 4 °C and the protein content of the supernatant determined using the Bradford method (Bio-Rad) in a spectrophotometer measuring the absorbance at 595 nm and then eluted in Laemmli 2X buffer with 8 % β-mercaptoethanol (95 °C for 5 minutes) ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were sonicated and then resolved by SDS-PAGE on 10% gels along with Precision Plus All Blue protein prestained standards (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 10 µg protein was then separated by SDS/PAGE and transferred to Immuno-Blot PVDF membranes (Bio-Rad). Membranes were incubated in 5% non-fat milk for 1 hour at room temperature to block nonspecific binding ...
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bioRxiv - Genetics 2022Quote: ... The 13 µl of protein was run on a 10% SDS-PAGE gel and transferred to a PDVF membrane by semidry transfer (Bio-Rad) at 13V for 2 hours 15 min ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed 3 times for 10 min in PBST before detection using Clarity Western ECL Substrate (Bio-Rad 170-5061) and imaging on a ChemiDoc MP (Bio-Rad 17001402).
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bioRxiv - Biochemistry 2022Quote: ... 0.2 μm nitrocellulose membranes were prewet for 10 minutes in TBS then secured into the Bio-Dot Microfiltration apparatus (Bio-Rad). Wells were rehydrated once with TBS ...
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bioRxiv - Biophysics 2022Quote: ... TIA1 LC domain was isolated from the supernatant using a Bio-Rad NGC Discover 10 chromatography system and a 5 mL Ni-affinity column (Bio-Rad Bio-scale Mini Nuvia IMAC or GE Healthcare HisTrap FF crude) ...
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bioRxiv - Biochemistry 2022Quote: ... SYPRO orange fluorescence data in relative fluorescence unit (RFU) was collected from 10 °C to 95 °C using CFX Connect Real-Time PCR Detection System (Bio-Rad). The temperature corresponding to the lowest point of the first derivative ...
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bioRxiv - Bioengineering 2022Quote: ... Libraries were then diluted to 10 nM and quantitated by qPCR on a CFX Connect Real-Time qPCR system (Bio-Rad) to confirm accurate pooling of barcoded libraries and to maximize the number of clusters in the flowcell ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins from each cell line were separated through SDS-PAGE (10% resolving gel) and electroblotted onto a polyvinylidene fluoride (PVDF) membrane using the semi-dry blotting system (Bio-rad). Next ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates (10-50 μg) were subjected to SDS-polyacrylamide gel electrophoresis on a 4-20% precast gradient gels (BioRad, 5678094) and proteins were transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein samples (cell lysates and DRG fractionized membrane and cytosol) were size separated using 10% or 4-20% SDS-PAGE gels (Bio-Rad), transferred to Immun-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
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bioRxiv - Developmental Biology 2020Quote: ... Total protein lysates were prepared from 25 pair of testes and loaded in equal dilution series (5, 10, and 20 μg) onto precast stain-free PAAG gel (Bio-Rad). After electrophoresis ...
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bioRxiv - Molecular Biology 2020Quote: Samples were separated using 10-15% SDS-PAGE gels or 4–20% Criterion TGX Stain-Free Protein Gel (Bio-Rad, 5678095). Gels were run at 100 V using 1 X SDS-PAGE Running Buffer [100 mL of 10 × 1 L Stock [30.2 g Tris Base (Fisher Scientific ...
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A Phytochrome B-PIF4-MYC2 Module Tunes Secondary Cell Wall Thickening in Response to Shaded LightingbioRxiv - Plant Biology 2021Quote: Proteins extracted from seedling samples were separated with 10% (w/v) SDS-PAGE gels and blotted onto polyvinylidene fluoride membrane (Bio-rad). Protein blots were then analyzed using either anti-GFP/Myc (1:2000 dilution ...
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bioRxiv - Immunology 2020Quote: ... Lysates (20–30 μL) were run on 8–10% polyacrylamide gels and transferred to PVDF membranes (Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were blocked with 5% BSA in TBS (TBS ...
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bioRxiv - Immunology 2020Quote: ... Equal amounts of protein were then resolved by SDS-PAGE on either 7.5% or 10% polyacrylamide gels (Bio-Rad, Hercules, CA). Separated proteins were transferred to PVDF Immobilon-FL membranes (Millipore ...
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bioRxiv - Bioengineering 2021Quote: ... all three active lasers engage in simultaneous excitation): forward scatter (“FSC”; SONY: 488/17 emission; BioRad: 488 excitation, 488/10 emission), back/side scatter (“BSC”/“SSC” ...
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bioRxiv - Microbiology 2021Quote: ... Total proteins (20 μg equivalent) were then separated by 10% or 12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE; Bio-Rad), then transferred by using 0.22-μm polyvinylidene fluoride (PVDF ...
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bioRxiv - Cancer Biology 2021Quote: ... Bound SRB dye was dissolved by adding 10 mM Tris-H2O to wells and OD was measured at 510nM with an iMARK microplate reader (Bio-Rad).
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bioRxiv - Developmental Biology 2022Quote: ... antibody-bead complexes were washed 3 times with lysis buffer before eluting the samples by boiling for 10 minutes in 4x loading dye (Bio-Rad) containing 10% β-mercaptoethanol ...
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bioRxiv - Physiology 2022Quote: ... we ran similar amounts of protein per lane (~10-20 μg, depending on target) on a 4-20% Criterion TGX stain-free gel (Bio-Rad) at 200 V for 50 min with the cassette surrounded by ice ...
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bioRxiv - Synthetic Biology 2022Quote: ... Reaction mixtures were incubated for 2 hours at 25 °C and quenched by adding 10 μL 4x Laemmli Sample buffer (Bio-Rad). Samples were boiled at 95 °C for 5 min and each 8 μL were loaded onto 12% SDS-PAGE gels ...
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bioRxiv - Immunology 2022Quote: ... boiled for 10 min and subjected to protein separation by SDS-PAGE in 4 – 20% Mini-PROTEAN TGX precast gels (Bio-Rad) before transferred to nitrocellulose membranes (Wako) ...
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bioRxiv - Microbiology 2022Quote: ... The gel was rinsed with DI water 3x for 10 min and imaged using a ChemiDoc Touch Imaging System (Bio-Rad) and processed by Image Lab software (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... 1 mM EDTA [pH 8.0]) On the final wash the supernatant was removed and 100 μL 10 % Chelex® 100 (w/v) (Bio-Rad) in MilliQ water was added the beads ...
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bioRxiv - Cell Biology 2019Quote: ... Equal amounts (15 µg) of protein lysates were heat denatured and separated on a 4–10% Mini-Protean Precast Gel (Bio-Rad), followed by transfer on a nitrocellulose membrane (Bio-Rad) ...
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bioRxiv - Plant Biology 2019Quote: ... Proteins were separated by SDS-PAGE in 10 or 12% SDS-polyacrylamide gels and transferred onto nitrocellulose membranes (0.45μm BioRad or 0.2μm Whatman) in a wet blotting apparatus ...
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bioRxiv - Pathology 2019Quote: ... and the resulting solutions were applied to a rehydration plate and covered with 17-cm immobilized pH gradient (IPG-immobilized pH gradient) strips for isoelectric focusing (pH 3-10, Bio-Rad). To soak the gel present on the strips with the protein sample ...
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bioRxiv - Cell Biology 2019Quote: ... with 10 ng of cDNA with specific primers of interest on a CFX Connect real-time PCR Detection System (Bio-Rad). Relative amounts of mRNA were calculated by comparative CT analysis with 18S ribosomal RNA used as internal control ...
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bioRxiv - Microbiology 2019Quote: ... Quantitative RT-PCR (qRT-PCR) reactions were performed in 10 μL volumes using 5 μL of SsoAdvanced Universal SYBR Green Supermix (Bio-Rad), 4 μL of a mix containing 0.5 μM of each transcript-specific primer (Table S2) ...
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bioRxiv - Molecular Biology 2019Quote: ... with a molecular weight cut off of 10 kDa and the final concentration was estimated using the Bradford protein assay (5000006, Bio-Rad). The protein was also subjected to mass spectrometry to assess its purity and molecular weight ...
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bioRxiv - Plant Biology 2019Quote: Equal amounts of the solubilized protein fractions were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). RFP-fused proteins were detected using a rat anti-RFP antibody (1:5,000 ...
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bioRxiv - Plant Biology 2019Quote: ... Equal amounts of the solubilized protein fractions were separated on 10% SDS-PAGE gels and blotted onto PVDF membranes (Bio-Rad). Protochlorophyllide oxidoreductase (POR ...
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bioRxiv - Biochemistry 2019Quote: ... and 100 μM Suc-LLVY-AMC with or without 0.02% SDS for 10 min before imaging on a Chemidoc™ MP Imaging System (Bio-Rad). The same gel was subsequently fixed and Coomassie stained for detection of total protein.
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bioRxiv - Microbiology 2019Quote: ... Reactions were run in triplicate in a Bio-Rad CFX96 Touch C1000 qPCR machine in 10 μl final volumes with 5 μl iTaq Universal SYBR Green Supermix (Bio-Rad), 0.5 μl of each primer and 1 μl DNA extract ...
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bioRxiv - Cell Biology 2019Quote: Tissue or cell extracts equivalent to 20 μg total protein were resolved in 10% SDS-PAGE gels followed by electrophoretic transfer onto a nitrocellulose membrane (Bio-Rad) in Tris-glycine buffer ...
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bioRxiv - Bioengineering 2020Quote: ... on Tris-Tricine gels (self casted 10% gels, as described in ref53) using an Mini-PROTEAN Tetra Cell system (Bio-Rad). For heterododecamer samples tris-tricine (Laemmli ...
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bioRxiv - Developmental Biology 2021Quote: ... Blots were then washed 3x 10 min in TBS followed by imaging of Stain Free total protein signal with a ChemiDoc MP System (Bio-Rad). Following stain-free imaging ...
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bioRxiv - Molecular Biology 2020Quote: Total proteins from HepG2 cells were prepared and run on 10% SDS-PAGE and transferred to a PVDF membrane using a transfer apparatus following the standard protocols (Bio-Rad). After incubation with 5% nonfat milk in TBST (10 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... Glycerol was added to the supernatants (final concentration: 10%) and protein concentrations were adjusted using Bio-Rad protein assay (Bio-Rad). The protein samples were diluted in 6x loading buffer (9.8% glycerol ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... or supernatants obtained after digitonin treatment were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) (73) ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were then mixed with 4x sample buffer and boiled for 10 min prior to separation by SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad) using a Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2021Quote: ... which contains 10 copies of the 601-positioning sequence and a linker region and purified using Model 491 Prep Cell (Bio-Rad). Annealed bubble templates and digested 601-R fragments were ligated using T4 DNA ligase (NEB ...
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bioRxiv - Neuroscience 2019Quote: ... 30 μg of each protein sample was denatured and reduced by boiling at 95°C for 5 min in 10% (v/v) 2-mercaptoethanol-containing Laemmli sample buffer (Bio-Rad). Samples were resolved by SDS-PAGE with a precast Tris-glycine gel (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... Dynabead-antibody complexes were washed 3x with 1 ml IP buffer and 3x with 1X PBS and proteins were eluted at 70°C for 10 min in 2X Laemmli sample buffer (50 μl; Bio-Rad) containing 5% 2-mercaptoethanol ...
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bioRxiv - Molecular Biology 2021Quote: ... Total proteins extracted with 1 X SDS were separated by 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to nitrocellulose membranes (1620112, Bio-Rad). The membranes were blocked in 5% BSA for 1 hour at RT and probed with primary antibodies ...