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Citations for Bio-Rad :
4051 - 4100 of 7321 citations for 4S 6 8 difluoro 3 4 dihydro 2H 1 benzopyran 4 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pharmacology and Toxicology 2020Quote: PCR Products were separated on a 0.8% agarose gel and visualized after staining with DNA Stain Clear G (SERVA, Heidelberg) on a ChemiDoc imaging system (Biorad, Germany).
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bioRxiv - Synthetic Biology 2021Quote: ... 10nM of templates with each promoter type were added to TXTL reactions and incubated at 30 °C for 8 hours (T100 Thermal Cycler, Bio-Rad). Post-incubation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed in Native NiNTA lysis buffer (50mM NaH2PO4, 300mM NaCl, pH 8, 10mM imidazole) and protein concentration was measured via Bradford assay (Bio-Rad). 500-800μg of protein were incubated with NiNTA beads at 4°C overnight ...
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bioRxiv - Genetics 2021Quote: ... or 1:8 (for all other quantifications) and 2ul was used to quantify gene expression using iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... cell lysates were separated by 8% SDS-PAGE and Western blotted onto a PVDF membrane (semidry transfer, 15 V, 45 min, Trans blot Bio-Rad). The membranes were incubated overnight at 4°C in blocking solution 5% (w/v ...
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bioRxiv - Neuroscience 2021Quote: ... Thirty micrograms of total protein from each sample were loaded on 8% SDS-PAGE and transferred to PVDF membranes (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Cell lysates were separated on 8-12% SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). The membrane was incubated overnight with the respective primary antibodies at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 50 μl of eluted samples were electrophoresed for 8 min at 200 V on a 10% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and protein was stained using Quick Blue Stain ...
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bioRxiv - Neuroscience 2022Quote: ... The pellet was resuspended in 50 μL 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Microbiology 2023Quote: ... and treated with or without heat denaturation (96°C, 8 min) and ran on a TGX stain-free precast gel (Bio-Rad) for 38 min at 200 V ...
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bioRxiv - Neuroscience 2022Quote: ... and 500 ng RNA (RIN>8) was transcribed to cDNA using the iScript first strand cDNA synthesis kit (Bio-Rad 1708891). qPCR was carried out on a 384-well Quantstudio Flex system (ThermoFisher ...
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bioRxiv - Plant Biology 2023Quote: ... The total proteins were separated by 8%-12% SDS-PAGE gels and blotted onto an Immune-Blot polyvinylidene difluoride (PVDF) membrane (Bio-Rad). All the antibodies were obtained from commercial suppliers (anti-GFP and anti-MYC antibodies from Roche ...
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bioRxiv - Immunology 2024Quote: ... Equal amounts of pre-cleared lysate samples were separated in 8 – 16% Mini protean-TGX stain free protein gel (Bio-Rad) and the gel was transferred to a Trans-Blot Turbo Mini-PVDF membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... Approximately 20-30 μg of protein was resolved on 8% polyacrylamide gels and transferred onto nitrocellulose membranes using semi-dry Trans-Blot TurboTM (Bio-Rad). Following antibody incubation (anti-GFP ...
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bioRxiv - Cell Biology 2024Quote: ... Equal amounts of protein (20-30 μg) were resolved by 8 to 15% SDS PAGE MiniPROTEAN® TGX gels (Bio-Rad), from samples previously prepared with Laemmli Buffer 4X™ (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... Blots were washed 8 × 5 minutes in TBST and then ladder lanes were reacted with Precision Plus Strep Tactin HRP Conjugate (BioRad 1610380) diluted 1:10,000 in TBST ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips (pH 5 to 8, Bio-Rad, Hercules, CA, USA) before separation by SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: ... and then the gel was washed in destain buffer (8% acetic acid) until the background was washed off for visualization by ChemiDoc Imaging system (Bio-Rad).
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bioRxiv - Immunology 2023Quote: ... 8 µg protein per condition were separated by SDS-PAGE and blotted onto a 0.2 µm nitrocellulose membrane (Bio-Rad, #1704158) using a Trans-Blot® Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... The protein extract (40μg) was separated on 8 or 10% polyacrylamide mini gel and transferred to a PVDF membrane (Bio-Rad, USA). Blotted membranes were incubated overnight at 4°C in SuperBlock(PBS ...
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bioRxiv - Bioengineering 2023Quote: We used conjugated magnetic beads (6.5 μm) (Bio-Plex Pro Human Chemokine IL-8 / CXCL8 Set #171BK31MR2, BioRad, Hercules, CA, USA) for the binding assay ...
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bioRxiv - Neuroscience 2022Quote: ... and then subjected to SDS polyacrylamide gel electrophoresis (PAGE) with 8% polyacrylamide and transferred to polyvinylidene difluoride (PVDF) membrane (BioRad Laboratories). The membranes were blocked for 1 h in Tris buffered saline with Tween 20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... were separated by 8% acrylamide SDS-PAGE and transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer kit (Bio-Rad). 5% (w/v ...
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bioRxiv - Cell Biology 2023Quote: ... Aliquots of cell lysates (40-60 μg of protein) were subjected to SDS/PAGE on an 8 to 15% (vol/vol) acrylamide gel (MiniProtean; Bio-Rad) including PageRuler Prestained Protein Ladder (Thermo) ...
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bioRxiv - Immunology 2023Quote: ... Even amounts of each protein sample were separated on a 8–10% polyacrylamide gel using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... Parasites were incubated with drug for 8 or 12 h (short or long assays, respectively) and stained with 5 μg/ml ethidium bromide (EtBr, Bio-Rad) for 10 min followed by immediate flow-cytometry analysis (Becton Dickinson ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were heated at 95°C for 5 minutes and cooled to room temperature before being separated on an 8% tris-glycine SDS-PAGE gel and transferred to a nitrocellulose membrane using a semi-dry transfer machine (Bio-Rad). Membranes were blocked in 5% skim milk dissolved in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 8M urea buffer (8 M urea, 100 mm NaHPO4, pH 8.5) and boiled in Laemmli sample buffer (BioRad, 161-0737) at 98°C for 5 min ...
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bioRxiv - Biochemistry 2024Quote: ... using gels with a concentration of 8%, 10%, 14% or 15% acrylamide (Carl Roth, 3029.1) in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell system (Bio-Rad, 1658029FC) with running buffer containing 0.2 M glycine (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The plate images were captured at time 0 and 8 hours post gel detachment using a ChemiDoc Touch Imaging system (Bio-Rad) and quantitated using Image J v1.54 software (NIH ...
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bioRxiv - Genomics 2024Quote: ... An equal amount of lysates per sample was loaded into a pre-cast 8-16% gradient Mini PROTEAN TGX gels (Biorad, 4561105), and proteins were transferred to the nitrocellulose membrane ...
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bioRxiv - Biophysics 2024Quote: ... Samples were separated on either 8% or 15% SDS-PAGE gels and transferred to nitrocellulose membranes using a Western blot apparatus (Bio-rad). Running buffer composition was 190 mM Glycine ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were loaded in an 8% SDS-PAGE gel and electrophoresis was conducted at 100 V in Tris/Glycine/SDS buffer (BioRad #1610732). Following ...
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bioRxiv - Cell Biology 2024Quote: ... to make 8 mL of 9% resolving Phos-tag gel solution (enough for one 1.5 mm Bio-Rad mini gel cast), the following reagents were mixed together evenly ...
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bioRxiv - Biophysics 2024Quote: ... The samples were separated using 8% SDS PAGE gels and then transferred onto a nitrocellulose membrane using standard Western blot apparatus (Bio-Rad). Kv1.2 was detected using a mouse monoclonal Kv1.2 antibody (1:10,000 dilution ...
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Single-molecule analysis reveals the mechanism of chromatin ubiquitylation by variant PRC1 complexesbioRxiv - Biophysics 2024Quote: ... Sucrose was added to achieve a final concentration of 8% and samples were loaded onto a ice-cold 5% Criterion TBE gel (Bio-Rad). Gel electrophoresis was performed in ice-cold 0.5× TBE buffer (4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... equal amounts of protein (10-20 µg) were resolved by 8% SDS–PAGE gels and transferred onto polyvinylidene fluoride membranes (Bio-Rad). The membranes were blocked in 4% (wt/vol ...
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bioRxiv - Cancer Biology 2024Quote: Samples were run through 8% SDS-polyacrylamide (SDS-PAGE, made in-house) via by gel electrophoresis (Bio-Rad; Hercules, CA, USA). The proteins were then transferred onto 0.45-μm polyvinylidene difluoride membranes (Milipore ...
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bioRxiv - Biophysics 2021Quote: ... Detergents were removed by adding 2-3 batches of Bio-beads SM2 (Bio-Rad) with constant rotation for overnight ...
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bioRxiv - Molecular Biology 2022Quote: ... and 3 µl was used for qPCR with iTaq Universal SYBR Green Supermix (BioRad). Relative levels of RNA were calculated by the ΔΔCt method using GAPDH as an internal control ...
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bioRxiv - Microbiology 2021Quote: ... The nitrocellulose membrane was soaked (blocked) in 3% milk (dry milk from Bio-Rad) solution in 1x PBS following by incubation with appropriate primary antibody solution (1:1000 dilution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... washed 3 times and developed with ECL reagent (Bio-Rad Laboratories, Hercules, CA, USA). Invertase blots were prepared similarly with an anti-invertase antibody and anti-goat IgG HRP ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE and Western blotting were performed using the Mini-PROTEAN 3 system (Biorad). Blotted membranes were blocked in 5% BSA / TBS-Tween ...
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bioRxiv - Immunology 2024Quote: ... Samples were diluted in Laemmli sample loading buffer supplemented with |3-mercaptoethanol (Bio-Rad), heated for 5 min at 95°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Following separation via SDS-PAGE (12%, Mini PROTEAN 3 System, Bio-Rad, Hercules, CA), proteins were transferred to PVDF membrane for 1hr using a Genie electroblot chamber (Idea Scientific ...
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bioRxiv - Physiology 2023Quote: ... Blotting membrane was primed for 3 min prior in cold transfer buffer (BIORAD 10026938), according to manufacturer specifications ...
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bioRxiv - Evolutionary Biology 2023Quote: ... kinase-dead extracts were incubated 3 consecutive times with protein A-beads (Bio-Rad) previously coated with the anti-B55δ antibody (1 hour incubation at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were loaded onto a 3% agarose gel and visualized on a Chemidoc (BioRad). Bands were densitometry quantified by ImageJ and the percentage of methylated DNA was calculated over the total intensity of all the quantified bands.
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bioRxiv - Molecular Biology 2023Quote: ... 3 μg of the protein samples were prepared with 4X Laemmli loading buffer (Biorad) and denatured at 95°C for 5 minutes ...