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Citations for Bio-Rad :
4001 - 4050 of 7321 citations for 4S 6 8 difluoro 3 4 dihydro 2H 1 benzopyran 4 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... RNA with a RIN value of at least 8 was used for cDNA synthesis using the iScript cDNA synthesis kit (BioRad) according to manufacturer’s protocol.
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bioRxiv - Cell Biology 2021Quote: Samples were run on 8-12% SDS-PAGE gel and transferred to a nitrocellulose membrane using a Trans-Blot Turbo Transfer System (BioRad). Membrane was blocked in 5% milk in TBS + 0.1% Tween blocking solution ...
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bioRxiv - Cell Biology 2022Quote: ... protein lysates were loaded onto 8% SDS-PAGE gel and transferred to a PVDF membrane using a semi-dry transfer system (Biorad) for 10 min at 2.5 amperes ...
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bioRxiv - Cell Biology 2022Quote: ... 10-30 μL of the supernatants (based on protein abundances) were run on an 8% SDS-PAGE gel and transferred to a nitro-cellulose membrane (Cat. No. 1620112, BioRad) using a semi-dry transfer method (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Extracted proteins (25-35 μg) were separated by 8-12% SDS-PAGE and transferred onto polyvinylidene difluoride membranes (PVDF, BioRad). Membranes were blocked for ~30 min in PBST (PBS and 0.1% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... All steps were done manually using 8-strip PCR tubes and PCR reactions were carried out on a 96-plate thermal cycler (Biorad). Libraries were pooled and sequenced on a Nextseq550 sequencer (Illumina ...
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bioRxiv - Neuroscience 2024Quote: Samples (8–25 μg protein extract) were run on 8–10% acrylamide gel at 100 Volt in Running Buffer (1X Tris–Glycine-SDS, Biorad) together with Precision Plus Protein Dual Color Standards (Biorad) ...
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bioRxiv - Bioengineering 2024Quote: ... Samples in 8-well tubes were heat-shocked for three minutes at 72 °C (C1000 Touch Thermal Cycler, Bio-Rad), placed on wet ice for two minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... were resolved on SDS 8-15% polyacrylamide gels and transferred onto nitrocellulose membranes using the Trans-Blot turbo transfer system (BioRad). Membranes were blotted with the primary antibodies against ADMA (Epicypher ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were boiled for 8 min at 95 °C in 1X Laemmli buffer (LB) with β-Mercaptoethanol (BioRad, 1610747), resolved on 10% polyacrylamide gels or pre-cast 4-12% Bis-Tris gradient gels (Thermo Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysate and dilutions were denatured in 1x Laemmli buffer at 95C for 30 seconds and resolved by SDS-PAGE on a 8-20% gradient gel (BioRad). Following trimming of the gel ...
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Effects of menopause and high fat diet on metabolic outcomes in a mouse model of Alzheimer’s diseasebioRxiv - Neuroscience 2023Quote: ... Diabetes-associated markers were assessed using the Bio-Plex Pro Mouse Diabetes 8-Plex Assay (171F7001M; Bio-Rad, Carlsbad, California) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2024Quote: ... 15-60 μg of denatured and reduced protein was subjected to 8-12% gradient SDS-PAGE followed by transfer to low-fluorescence polyvinylidene difluoride membranes (LF PVDF, BIORAD). Membranes were blocked with LICOR Odyssey TBS Blocking Buffer (LI-COR ...
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bioRxiv - Immunology 2024Quote: ... were combined with Pierce Lane Marker Reducing Sample Buffer (Thermo) and applied onto SDS gels (Mini-PROTEAN TGX Gels 8-16%, BioRad for 24h/48 h/72h samples and a 10% SDS gel for resistance validation) ...
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bioRxiv - Molecular Biology 2024Quote: ... with an 8-minute incubation at 95°C using a C1000 Touch Thermal Cycler (Bio-Rad, cat. no.185-1196). The cleaved and primed RNA was reverse transcribed into first strand cDNA using SuperScript II Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... The target genes were amplified from 8 ng of gDNA template with CFX96 Touch Real-Time PCR (Bio-Rad, USA) under the following conditions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein quantifications were determined with a Bradford assay then 30μg of protein was separated on a 8-16% or 12% pre-cast gel (Bio-Rad) using primary antibodies diluted 1:500-1:1000 and secondary antibodies (1:2500 ...
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bioRxiv - Cell Biology 2021Quote: ... Blocking solution consisted in 3% nonfat dry milk (Blotting-Grade Blocker, BIO-RAD Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... were resolved by 10% denaturing PAGE in Mini Protean 3 Cell (Bio-Rad). Separated RNA was then transferred to a nylon membrane (Hybond-XL ...
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bioRxiv - Molecular Biology 2021Quote: ... with 3 uL Precision Plus Protein All Blue prestained standards (Bio-Rad, #1610373) loaded as a ladder ...
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bioRxiv - Genomics 2022Quote: ... and 3 μl of 2x iProof HF Master Mix (BioRad, Hercules, CA, USA). The 3 μl of 2x master mix was dispensed using a Mantis Liquid Handler (Formulatrix ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 µl DTT and 1000 µl Purezol (Biorad, USA, CA, Cat No: 7326890) were added to each falcon tube and kept on ice for 5 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... on an Opticon 2 from MJ Research with Opticon Monitor 3 software (BioRad). Reactions were set up manually using an 8-channel pipette (30-300μL ...
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bioRxiv - Bioengineering 2024Quote: 3⋅108 total particles were loaded into pre-cast gels (BioRad, Cat. # 4561094). Samples were blocked in a 5% nonfat milk solution and stained for the common EV marker CD9 using a primary antibody (Cell Signaling Technologies ...
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bioRxiv - Bioengineering 2024Quote: ... and Precision Plus Protein Dual Color Standards (1610374, Bio-Rad, 3 μL/well). All antibodies used for western blotting were diluted at a ratio of 1:1000 or 1:500 unless otherwise specified ...
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bioRxiv - Cancer Biology 2024Quote: ... and Bio-Plex Pro™ Mouse TGF-β 3-Plex assays (Bio-Rad) were utilized to profile the concentrations of mouse cytokines and chemokines in the cell culture supernatants ...
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bioRxiv - Biochemistry 2022Quote: ... and 0.032 % (w/v) SM was applied to a centrifugal device (Micro Bio-Spin 6, Bio-Rad, Hercules) to exchange the buffer to a MS buffer containing 200 mM ammonium acetate (pH 7.4) ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA was purified by loading quenched samples onto Micro Bio-spin Columns with Bio-Gel P-6 (BioRad), followed by ethanol precipitation ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by 6 washes in 100 µL of PBST in Bio-Spin columns (catalog number 7326204, Bio-Rad). The bound immunocomplexes were eluted via 0.1 M glycine ...
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bioRxiv - Biochemistry 2021Quote: KirBac3.1 protein was buffer-exchanged to 200 mM ammonium acetate (pH=8.0) with 0.5% C8E4 (2×CMC) using a Biospin-6 column (BioRad). The protein sample was loaded into a gold coated needle prepared in-house and analysed on a modified Q-Exactive mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2022Quote: ... then washed 6 x 5 minutes with TBS-T before developing with Clarity Western ECL Substrate (Bio-rad).
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bioRxiv - Biochemistry 2022Quote: ... solutions containing purified CytbX were buffer-exchanged into 0.2 M ammonium acetate (pH 8.0) containing 2x CMC of Lauryldimethylamine oxide (LDAO) using Biospin 6 columns (Biorad). 2-3 µL of 5 µM protein solution was introduced directly into Q-Exactive UHMR mass spectrometer (ThermoFisher ...
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bioRxiv - Biophysics 2022Quote: ... then 6 L of RO water with Ca2+ chelating beads (BIO-RAD Chelex 100 Resin Cat#142-1253), and finally 6 L of RO water alone ...
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bioRxiv - Biochemistry 2023Quote: ... Protein was then cleaned 2-times on Micro Bio-Spin™ P-6 gel Columns (BioRad, #732-6221) where buffer was exchanged with phase separation buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... sampels were separated on 6– 15% SDS–PAGE and proteins were transferred to nitrocellulose membrane (1620115, Bio-Rad) using the Trans-Blot Turbo Transfer System from Bio-Rad ...
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bioRxiv - Synthetic Biology 2024Quote: ... Images were acquired using a Chemidoc Imaging System and analyzed with Image-Lab v.6 software (Bio-Rad).
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The triad interaction of ULK1, ATG13, and FIP200 is required for ULK complex formation and autophagybioRxiv - Cell Biology 2024Quote: ... and 150 mM NaCl utilizing a Bio-Scale Mini Bio-Gel P-6 desalting column (Bio-Rad Laboratories). Subsequently ...
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bioRxiv - Cancer Biology 2024Quote: ... Precision Plus Protein Dual Color Standards Protein Marker was used as a marker (6 μl, #1610374, BIO-RAD).
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bioRxiv - Synthetic Biology 2021Quote: ... The culture supernatant was obtained by centrifugation and used with the Bio-Plex Pro Human Cytokine 8-plex Assay (Bio-rad) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies) and in Criterion cells (Bio-Rad) using respective adapters (LifeTechnologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... loaded on SDS-polyacrylamide gels (8 to 10 %) and transferred to nitrocellulose membranes using Trans-blot turbo transfer system (Bio-Rad). Membranes were saturated for 1 h with TBST buffer (150 mM NaCl ...
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bioRxiv - Biochemistry 2020Quote: ... concentrated the supernatant on a 3-kDa cutoff centrifugal filter unit (Amicon) and loaded samples of supernatant on a 8-16% SDS-PAGE TGX Stain-Free gels (Bio-Rad) (SI Figure 1c).
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bioRxiv - Plant Biology 2020Quote: ... then spun down at 12.000g for 15 minutes to remove insoluble material and subsequently analyzed using 12% (in-house gels) or 8-16% SDS-polyacrylamide gels (Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... 10 to 30μg of total protein were subjected to 8% or 10% SDS-PAGE gels and transferred electrophoretically onto PVDF membrane by a semi-dry blotting system (Bio-Rad). The membrane was blocked in 5% fat-free milk/Tris-buffered saline for 1 hour and incubated with primary antibodies at 4°C overnight followed by horseradish peroxidase conjugated secondary antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... and 25-100 µg of total protein was loaded onto 8 to 15 % SDS-PAGE minigels (Bio-Rad Laboratories, Hercules, CA) prior transfer onto PVDF membranes ...
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bioRxiv - Neuroscience 2021Quote: ... were added to the 8 channel droplet generation cartridge according to manufacturer instructions and this cartridge was placed in the QX200 droplet generator (Bio-Rad). Then ...
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bioRxiv - Immunology 2021Quote: ... and 2D-sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) replicates performed on 11cm 8-16% (w/v) polyacrylamide/Tris-HCl gels (Bio-Rad) using a Criterion Dodeca Cell (Bio-Rad) ...
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bioRxiv - Immunology 2020Quote: ... Lysates (20–30 μL) were run on 8–10% polyacrylamide gels and transferred to PVDF membranes (Trans-Blot Turbo Transfer System, Bio-Rad). Membranes were blocked with 5% BSA in TBS (TBS ...
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bioRxiv - Microbiology 2020Quote: ... Flow cytometric staining and analysis was done as described [8] and acquired on ZE5 cell analyzer (Bio-Rad, Hercules, CA, USA). Analysis was performed using Flow Jo software.
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were separated by isoelectric focusing on immobilized linear pH gradient strips pH 5-8 for CDK4 (BioRad, Hercules, CA, USA) or pH 3–10 for CDK2 (Amersham Biosciences ...