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Citations for Bio-Rad :
4201 - 4250 of 7321 citations for 4S 6 8 difluoro 3 4 dihydro 2H 1 benzopyran 4 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies (Table 3) were diluted in 5% non-fat blocking milk (BioRad, Cressier, Switzerland) in TBST and incubated over night at 4°C with mild agitation ...
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bioRxiv - Plant Biology 2024Quote: ... TCA 3% (v/v)] and pictures were taken using a GelDoc XR imaging system (Biorad). The membranes were then washed in Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2024Quote: ... 3 μL of Precision Plus Protein™ dual color standard weight ladder (#1610374, Bio-Rad) were also loaded onto the gel ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... while insulin levels were assayed using the quantitative Bio-Plex Pro™ Mouse Diabetes 8-Plex immunoassay (#171F7001M; Bio-Rad Laboratories, Hercules, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Twenty microlitres of the mastermix + template DNA were pipetted into the very bottom of the central row of (8) wells of the Droplet Generator DG8™ Cartridge (Bio-Rad 186-4008). Sixty-five microlitres of droplet generation oil for probes (BioRad 186-3005 ...
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bioRxiv - Biochemistry 2020Quote: ... resolved on the 8% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 µm pore size) with the use of a Trans-Blot Turbo Transfer Starter System (Bio-Rad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Molecular Biology 2022Quote: ... cell viability was determined using the Cell Counting Kit-8 (Dojindo, Kumamoto, Japan) by measuring the absorbance at 450 nm using an iMarkTM microplate absorbance reader (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Neuroscience 2023Quote: ... Forty µg denatured protein per well was resolved by 8-12% SDS- PAGE homemade gels in parallel with multi-colored molecular weight marker (Bio-Rad Laboratories, Cat.No.1610395). Gels were run at 100V for 1-2 h and transferred overnight at 8- 12V ...
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bioRxiv - Physiology 2024Quote: ... 10-20 µg tissue lysates were separated by 8% gel in SDS-PAGE and transferred to nitrocellulose membranes (Trans-Blot, Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% nonfat dried milk or 3% BSA (for phosphorylated proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... the plate was held at 4 °C until the lid temperature cooled to 37 ºC and could be stored at 2 °C to 8 °C overnight before analysis on the QX600 Droplet Reader (Bio-Rad Laboratories, California, USA).
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bioRxiv - Bioengineering 2020Quote: ... Equal volumes of 10 mg/L (GT)15- or (GT)6-Cy5-SWCNTs and 160 mg/L protein were added to a 96-well PCR plate (Bio-Rad) to a total volume of 50 μL ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (20 µg/lane) were electrophoresed on a 6–12% SDS polyacrylamide gel in a Mini-PROTEAN Tetra Cell (Bio-Rad) and subsequently transferred to a nitrocellulose membrane (Sartorius AG ...
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bioRxiv - Cell Biology 2021Quote: ... inside an anaerobic glove-box and the excess DTT was completely removed via a Bio-Gel P-6 DG gel-desalting column (Bio-Rad) in thoroughly de-oxygenated MOPS buffer (20 mM ...
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bioRxiv - Biophysics 2021Quote: ... DIV15-30 neurons were transfected 2-6 days before imaging via biolistic gene transfer (160 psi, Helios gene gun, Bio-Rad), as described in a detailed video protocol (Woods and Zito 2008) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... were then generated using the piggyBac transposon system as described.12 Protein expression was induced with doxycycline and secreted fusion protein was separated from expression media using IgG Sepharose 6 Fast Flow resin (GE-Healthcare) in a 10 mL Poly-Prep chromatography column (Bio-Rad). Resin was washed with 50 column volumes of wash buffer (10 mM Tris pH 7.5 ...
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bioRxiv - Immunology 2020Quote: ... The concentrations of mouse and human IL-6 and TNF-α were determined by a multiplex Bio-Plex assay (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... cells were infected with SINV at an MOI of 2 and pictures were taken 6 hours post-infection using ZOE fluorescent cell imager (Bio-Rad). Proteins were collected with lysis buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... was incubated at 55°C for 2 h and then purified by 6% non-denaturing PAGE using a Prep Cell apparatus (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Membranes were dried out at RT and then exposed to a phosphoscreen cassette (GE) for 6 days for further scanning in a phosphoimager analyzer (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... chromosomal DNA plugs were prepared and separated on 1% agarose gel at 6 V/cm for 48 hrs (initial time = 20s, final time = 30s) by using the CHEF DRII apparatus (Bio-Rad), followed by Southern blotting and hybridization with a TRP1 sequence as a probe.
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bioRxiv - Biochemistry 2023Quote: ... The 6× His-tag blots were washed with TMST and TSM and developed with the Clarity Western ECL kit (Bio-Rad) and imaged in a Gel-Doc system (Bio-Rad) ...
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bioRxiv - Immunology 2024Quote: ... The gel was imaged under the Silver Stain setting on Image Lab 6 on a Gel Doc EZ Imager (Bio-Rad). ImageJ was used to quantify band intensities and determine peptide loading efficiencies.
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β-catenin programs a tissue-specific epigenetic vulnerability in aggressive adrenocortical carcinomabioRxiv - Cancer Biology 2022Quote: Samples were prepared by boiling at 95°C for 6 minutes in lysis buffer supplemented with 4x Laemmli Sample Buffer (Bio-Rad), with 355 mM 2-mercaptoethanol freshly added according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... or immunoprecipitated samples were resolved by SDS-PAGE (6% to 10%) and transferred onto methanol-treated immun-blot PVDF membrane (Bio-Rad). The membranes were incubated overnight at 4°C with corresponding primary antibodies (antibody information ...
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bioRxiv - Physiology 2023Quote: ... The incorporated radioactive methionine and cysteine signal was detected using Typhoon Imager (GE) and quantified using Image Lab 6 software (Bio-Rad). Afterwards ...
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bioRxiv - Cell Biology 2023Quote: Samples were diluted in 5x loading buffer and ∼30 mg run out on a 6-14% SDS-PAGE gel as described before semi-dry Western blotting (BioRad TurboID). Ponceau stained images were taken ...
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bioRxiv - Cancer Biology 2023Quote: ... Each sample was separated by non-reducing SDS-PAGE (6% acrylamide gel) with Precision Plus Protein Dual Color Standards (Bio-Rad) and transferred to nitrocellulose membrane overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs isolated from two donors were infected with GFP-EBV for 28 days in 6-well plates and subsequent B-cells blasts were photographed using using a ZOE Fluorescent Cell Imager (BIO-RAD). Using similar strategy ...
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bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
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bioRxiv - Plant Biology 2024Quote: ... 500 μL of protoplasts in a total volume of 600 μL electrotransfection buffer were electrotransfected with 2-6 μg plasmid DNA using the Gene Pulser XcellTM (Bio-Rad) with a pulse at 160 V for 10 ms ...
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bioRxiv - Cell Biology 2024Quote: ... The maleamide reaction was quenched with 5 mM DTT and unreacted Halo Ligand and Quencher were removed using two Bio-Spin 6 (Tris) columns (Bio-rad).
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bioRxiv - Biochemistry 2024Quote: ... or a combination of both was added for 6 hours prior to imaging with either a ZOE Fluorescent Cell Imager (Bio-Rad) or EVOS FL Cell Imaging System (Thermo Fisher Scientific ...
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bioRxiv - Synthetic Biology 2024Quote: ... The gel obtained was imaged using the Silver Stain setting on Image Gel 6 on Gel Doc EZ Imager (Bio-Rad).
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bioRxiv - Evolutionary Biology 2024Quote: Protein samples were buffer exchanged into 200mM ammonium acetate using either a centrifugal buffer exchange device (Micro Bio-Spin P-6 Gel, Bio-Rad) or a dialysis device (Slide-A-Lyzer MINI Dialysis Unit ...
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative real-time PCR was performed on an Applied Biosystems QuantStudio 6 Flex Real-Time PCR System using iTaq Universal SYBR Green Supermix (Bio-Rad). 18S rRNA was used as an internal control ...
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bioRxiv - Developmental Biology 2022Quote: ... Membranes were washed 3 more times in TBST and imaged on a ChemiDoc Imager (Bio-Rad) using Clarity western ECL substrate (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Plates were imaged after 3 days at 30°C using the Chemidoc Touch imaging system (BioRad) on colorimetric setting.
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bioRxiv - Genetics 2021Quote: ... were determined using Bio-Plex Pro TGF-β 3-Plex Assay (171W4001M) (Bio-Rad, Hercules, CA).
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bioRxiv - Neuroscience 2020Quote: ... the soluble fraction from 3 months old APPPS1 mice have been quantified using Bradford assay (Biorad) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Blots were washed 3 times with TBST and then incubated with Clarity ECL substrate (Bio-Rad). Blots were imaged using a ChemiDoc MP gel doc system (Bio-Rad) ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were then wash 3 × 10 min with TBST and detected using chemiluminescence substrate (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... Samples were resolved on 7 cm pH 3-10 immobilized pH-gradient (IPG) strips (Bio-Rad), under mineral oil ...
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bioRxiv - Cell Biology 2021Quote: ... The beads were washed 3 times and then eluted using 2 × Laemmli Sample Buffer (Bio-Rad). The elution was subjected to SDS PAGE to run the sample into the lane ...
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bioRxiv - Systems Biology 2022Quote: ... Each aliquot was heated to different temperatures for 3 minutes in a PCR machine (Bio-Rad), with the temperature range being 42-64 °C and 2-degree intervals ...
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bioRxiv - Biochemistry 2020Quote: ... rinsed 3 times and read by the Bio-Plex (Bio-Rad, Bio-Plex MAGPIX Multiplex Reader). Each experiment was done in duplicate and repeated with media from 3 independent experiments (n=3).
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bioRxiv - Systems Biology 2020Quote: ... The samples were digested for 3 hours in a thermal cycler at 37 °C (BioRad T1000). Samples were cooled to room temperature before TMT labeling (TMT11 plex kit & TMT-Pro 16plex ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were eluted using a 20:7:3 mixture of buffer: 4x Laemmli sample buffer (Biorad):10x NuPage sample reducing agent (Invitrogen ...