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Citations for Bio-Rad :
4301 - 4350 of 7321 citations for 4S 6 8 difluoro 3 4 dihydro 2H 1 benzopyran 4 ol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... culture were then rinsed three times with PBS and treated with 3% (w/v) hydrogen peroxide (BioRad) in PBS for 5 min to minimize endogenous peroxidase activity ...
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bioRxiv - Genetics 2023Quote: ... and orange G) and ran for 3 h at 100 V with a Variable Speed Pump (BioRad) to circulate buffer during the entire gel run.
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bioRxiv - Biophysics 2023Quote: Apoptosis was probed using the Bio-Rad Magic Red Caspase 3-7 kit (Bio-Rad, ICT 935). HEK 293T cells stably expressing FGFR1-YFP were seeded in 96 well plates and allowed to grow to ∼70% confluency ...
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bioRxiv - Biochemistry 2024Quote: ... Membranes were then washed 3 times with TSB-T before revelation using ECL reagent (Bio-Rad 1705061) and high-resolution image acquisition using ImageQuant LAS 4000 (GE Healthcare Life Sciences).
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bioRxiv - Synthetic Biology 2024Quote: ... The gel was rinsed 3× with ultrapure water and imaged in a GelDoc Go imager by BioRad Laboratories ...
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bioRxiv - Cancer Biology 2020Quote: ... an equal amount of proteins was electrophoresed on 8 to 15% sodium dodecyl sulfate-polyacrylamide gels prepared in-house and transferred to polyvinylidene difluoride (PVDF) (Bio-Rad, Hercules, CA, USA; Cat# 1620177) membrane at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... and their respective complexes with Hap40 protein were buffer-exchanged into 150 mM aqueous ammonium acetate (pH=7.5) by using P-6 Bio-Spin gel filtration columns (Bio-rad, Veenendaal, the Netherlands). The protein’s resulting concentration was estimated to be ~2-5 μM before native MS analysis ...
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bioRxiv - Genetics 2022Quote: ... The DNA was diluted to 6 ng/µl for copy number screening and qPCR was performed using SsoAdvanced™ Universal SYBR® Green Supermix (Biorad, 1725272) and 1µM primer mix to amplify the target within and flanking the deleted region ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (10-100 μg proteins/lane) were loaded to and separated on 6%-15% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620177, Bio-Rad, Hercules, CA). The membranes were blocked in 4% BSA/PBST (0.1% Tween-20 ...
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bioRxiv - Molecular Biology 2022Quote: ... was performed for 23 h at 14°C with an initial switch time of 60 s and a final switch time of 120 s at 6 V/cm and an included angle of 120° as described previously (Bio-Rad CHEF Mapper) [35] ...
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bioRxiv - Immunology 2024Quote: ... ACPA IgG levels were quantified based on an in-house standard of pooled RA patient plasma using the Microplate manager software MPM-6 (Bio-Rad Laboratories, Inc.). Unspecific reactivity to the peptide backbone was assessed by applying CArgP2 ...
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bioRxiv - Physiology 2023Quote: ... 18 and 21 months of age and were subjected to IEF using a 24 cm immobiline DryStrip (pH 6-11) (BIO-RAD, Hercules, CA), followed by SDS-PAGE using pre-casted 10-16% gradient polyacrylamide gels (BIO-RAD ...
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bioRxiv - Biophysics 2023Quote: Proteins samples were kept on ice and buffer exchanged into 200 mM ammonium acetate buffer at pH 7 using Micro Bio-spin columns with either a 6 or 30 kDa cutoff (Bio-Rad, Hercules, CA). Buffer exchanged samples were then diluted to a working concentration of 10 μM before analysis ...
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bioRxiv - Cell Biology 2021Quote: ... Plates were incubated at 30°C for 3-5 days and photographs were taken by Chemi Doc (BioRad).
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bioRxiv - Plant Biology 2021Quote: ... After washing 3 times with TBST the chemiluminescent was conducted by adding Clarity Western ECL Substrate (Biorad, #1705061) for 2 min and detected by ChemiDoc imaging system (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: ... 40 μg of samples 3 were mixed with 2× laemmli buffer + 5% 2-mercaptoethanol (BioRad Laboratories, Hercules, CA). Samples were then briefly vortexed and centrifuged ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were again washed 3 times in PBST before being incubated with Clarity™ ECL reagents (Bio-Rad) or SuperSignal™ West Femto Maximum Sensitivity Substrate and visualized (see S11 Fig for full length immunoblots).
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bioRxiv - Neuroscience 2020Quote: ... and run at 60mV for 3 hours using the mini Protean II gel electrophoresis apparatus (Bio-Rad Laboratories). Protein was transferred to a nitrocellulose membrane (Hybond ECL ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed 3 times in TBSt and then incubated with HRP secondary antibodies (anti-Mouse HRP, Biorad, 170-6516 ...
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bioRxiv - Cell Biology 2022Quote: ... pH 7) and Western blotting samples were prepared by adding sample buffer (3× Laemmli Sample Buffer 1610747; BioRad) plus 3.57 M β-mercaptoethanol (2-mercaptoethanol ...
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bioRxiv - Microbiology 2022Quote: ... The purified 3-cycle PCR products were qPCR quantified using Platinum SuperFi II on a CFX96 system (BioRad). The same amount of the seven purified 3-cycle PCR products were further amplified using Platinum SuperFi II and the index primers from Nextera XT Index Kit v2 (Illumina cat ...
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bioRxiv - Plant Biology 2022Quote: ... 12.5 mM imidazole) for 3 times and boiled at 95°C with 30 μl SDS loading dye (BioRad). After removing the beads ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Molecular Biology 2024Quote: ... Plates were incubated for 3 days at 30 °C and imaged in a Gel Doc XR+ (Bio-Rad).
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bioRxiv - Immunology 2024Quote: ... cDNA was mixed with appropriate primers (Supplementary Table 3) and SYBR green master mix (BioRad, catalog no. 1708882) and run on a Thermocycler T100 (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... The membrane was then washed in TBST (3 times) and developed with ECL western blot substrate (Bio-Rad) and imaged using Azure C300 imaging system (Azure biosystems) ...
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bioRxiv - Molecular Biology 2023Quote: ... we performed 3 washes with PBST for 10 minutes before imaging the membrane on a ChemiDoc system (BioRad). The original scans of the blots are provided in Supplemental Table 3.
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bioRxiv - Molecular Biology 2024Quote: ... Only clones verified from both upstream and downstream genome-insert junctions were further analyzed for mCherry copy number by ddPCR (CN < 1.5 = single copy per genome, CN ≥ 2.5 = 3 or more copies per genome; Biorad). Then clones with 2 copies per genome had the target site amplified by BioTherm DNA polymerase (GeneCraft ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were then washed with TBST 3 times and imaged using the ChemiDoc MP imaging system (Bio-Rad) after activation with ECL substrate (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... Membranes were blocked in 3% nonfat dry milk in TBST (Blotting-grade blocker nonfat dry milk; Bio-Rad) at room temperature for 1h ...
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bioRxiv - Physiology 2024Quote: ... Twenty-μg protein samples were separated by SDS-PAGE using a Mini-Protean 3 Cell system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2024Quote: ... membrane was washed 3 x 10 min in PBS-T and imaged for chemiluminescence using ChemiDoc system (Biorad) and SuperSignal West Femto Maximum Sensitivity Substrate (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... was applied to the membrane for 3 min and then imaged using a chemiDoc imaging system (Bio-Rad). Image lab (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... at 90-120 Volts for 60-90 minutes in a Mini-Protean® 3 Cell (Bio-Rad Laboratories). Gel was transferred at 350 Amperes for 120 minutes onto pre-activated polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... Pax-3 and Pax-7 were quantified through Real-Time PCR using SYBR® Green dye (BioRad, USA) (Supplementary Table 1) ...
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bioRxiv - Cell Biology 2024Quote: ... Gels with Young’s modulus of 1.2 kPa were made by preparing a solution of 3% acrylamide (Bio-Rad), 0.11% bisacrylamide (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: ... and 3.6 μL ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The 2-ΔΔCt method was used for the quantitative analysis ...
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bioRxiv - Evolutionary Biology 2020Quote: ... was extracted from several colonies of a 6-week-old subculture on Coletsos culture medium using the InstaGene matrix following the manufacturer’s instructions (Bio-Rad, Marnes-la-Coquette, France). Then ...
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bioRxiv - Molecular Biology 2021Quote: ... The resulting transcripts were treated with DNase for 20 min at room temperature and then isolated using Micro Bio-Spin™ 6 Columns (BioRad, Cat.# 732-6221). 0.75 µg of RNA transcripts were mixed with 2X formamide buffer (95% deionized formamide ...
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bioRxiv - Biochemistry 2020Quote: ... the SL2-6 construct was subsequently size purified using a denaturing polyacrylamide gel (7M Urea, 1x TBE, hand-poured in Bio-Rad Criterion midi cassettes), loaded in 80% formamide ...
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bioRxiv - Immunology 2022Quote: ... Protein extracts were separated on 8 – 12 % polyacrylamide gels by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA, 0.2 µm pore size) in a wet transfer approach using the Mini Trans-Blot® cell (Bio-Rad) ...
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bioRxiv - Bioengineering 2022Quote: ... and the equivalent of 10 μg of chlorophyll was loaded per lane on a Tris-Glycine based SDS-gel (Mini-Protean®TGX, Bio-Rad or Novex™ 8%, Thermo Fisher) and transferred onto a PVDF membrane using the Trans-Blot®Turbo™ Transfer System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2021Quote: ... the membranes were washed 3 times for 10 minutes each and coated with enhanced chemiluminescence (ECL; Clarity ECL, BioRad). Using X-ray film ...
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bioRxiv - Cell Biology 2020Quote: ... Cell cycle of cells (> 3 × 104) were determined by HOECHST fluorescence in single cell by ZE5 cell analyzer (BioRad) and data were processed by Flowjo.
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bioRxiv - Biophysics 2020Quote: ... the membrane was washed 3 times with PBST and incubated with an ECL Western blot Reagent (1705060, Bio-Rad), for 5 min in the dark ...
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bioRxiv - Microbiology 2022Quote: ... NS5B-FAM probe: 5’-ATGGGTTCGCATGGTCCTAATGACACAC-3’) and the GAPDH loading control (PrimePCR Probe assay with HEX probe, Bio-Rad). Each 20 μL reaction contained 500 ng of total RNA ...
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bioRxiv - Molecular Biology 2020Quote: ... Gel electrophoresis was performed using the BioRad Mini-PROTEAN 3 gel electrophoresis system (Bio-Rad Laboratories, Hercules, CA, USA). Proteins were transferred to nitrocellulose membranes (Amersham™ Protran™ ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were subsequently washed 3 times in lysis buffer and bound complexes were eluted using 4x Lämmli sample buffer (Biorad). Samples were boiled at 95°C for 7 minutes and resolved through SDS-PAGE prior to Western blotting or analysed by quantitative Mass Spectrometry as described in the label-free LC-MS/MS analysis section ...
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bioRxiv - Biochemistry 2020Quote: ... using a DNA Engine Opticon 3 real time PCR system (MJ Research) with Opticon Monitor 3.1 analysis software (BioRad).
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bioRxiv - Evolutionary Biology 2020Quote: ... then transferred semi-dry for 7 min at 1.3 mV using Trans-Blot Turbo 0.2 μm nitrocellulose transfer packs and the Trans-Blot Turbo transfer system (BioRad). Blots were blocked with Odyssey blocking buffer (LI-COR ...