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Citations for Bio-Rad :
3901 - 3950 of 7302 citations for Superoxide Anion Microplate Assay Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: iScript Advanced cDNA Synthesis Kit for PCR (BioRad) was used for Reverse Transcription (RT ...
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bioRxiv - Genomics 2024Quote: ... iScript cDNA Synthesis Kit (Bio-Rad, Hercules, CA) was used to reverse transcribe one βg total RNA according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The iScript Reverse Transcription Supermix kit (Bio-Rad) was used to synthesize cDNAs from 200 ng of RNA according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... RNA was extracted with the Nucleospin RNA Isolation Kit (Machery-Nagel) and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). TaqMan real-time qPCR assays were used for quantification of human IFNB1 (Hs01077958_s1) ...
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bioRxiv - Immunology 2021Quote: Total RNA was extracted using the PicoPure RNA Isolation kit and cDNA synthesis was performed using the iScript cDNA synthesis kit (Bio-Rad). cDNA samples were amplified with SYBR Select mastermix (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA for PCR analyses was made with a commercially available kit (iScript™ cDNA Synthesis Kit, Bio-Rad Laboratories). The quantification of mRNA expression was performed using the SYBR Green dye (Bio-Rad SsoAdvanced™ - Universal SYBR® Green Supermix ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated either from homogenized mosquitoes or infected cell supernatant using a Multisource RNA Miniprep Kit (AP-MN-MS-RNA-250, Axygen) and reverse transcribed to cDNAs using an iScript cDNA Synthesis Kit (1708890, Bio-Rad). Viral genomes were quantified by qPCR using iTaq Universal SYBR Green Supermix (1725121 ...
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bioRxiv - Microbiology 2021Quote: ... using the primer-probe mix from the 2019-nCoV RUO kit (IDT) with iTaq Universal Probes One-Step kit (Bio-Rad). The 2019-nCoV_N_Positive Control plasmid (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was reversely transcribed with 0.5–1 μg RNA using the iScript cDNA synthesis kit as described in the kit (Biorad, Hercules, CA). The gene expression of Il-6 ...
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bioRxiv - Cell Biology 2021Quote: RNA was extracted using the RNeasy plus micro RNA extraction kit followed by reverse transcription using iScript cDNA synthesis Kit (Bio-Rad). Equal amounts of DNA and corresponding primers (Supplementary Table 1 ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted using the NucleoSpin RNA Plus Kit (Machery-Nagel) followed by reverse-transcription with the iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... 1μg of total RNA was reverse transcribed into cDNA using a high-capacity cDNA reverse transcription kit (iScript cDNA Synthesis Kit, Bio-Rad) according to the provided protocol ...
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bioRxiv - Microbiology 2024Quote: ... Complementary DNA (cDNA) was synthesized using a Qscript kit (QuantaBio, MA) as per kit instructions in a T-100 (Bio-Rad) thermocycler.
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bioRxiv - Evolutionary Biology 2021Quote: ... The concentration of total protein mite extracts was determined using the Quick Start Bradford Protein Assay (Quick start Bradford 1x dye reagent, Bio-Rad, Cat# 500-0205), with Bovine Serum Albumin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Then ran the assay in accordance with the specification (Bio-Plex Pro Mouse Cytokine Grp, Catlog NO. #M60009RDPD, LOT. 64212941, Bio-Rad Laboratories, Inc., USA). Read the plate at Bio-Plex MAGPIX System (Bio-Rad Laboratories ...
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bioRxiv - Developmental Biology 2020Quote: ... Bradford Protein assay method was used to determine the protein concentration using bovine serum albumin (BSA) as the standard (Bio-Rad catalog 500-0006). 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: The supernatants of cell patches were collected after culturing under normoxic or hypoxic (5% O2) conditions and analysed using a fluorescence-dyed microsphere-based immune assay (Bio-Rad Laboratories, Hercules, CA) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were clarified for 5 min at 10,000 × g at +4□C and protein concentration was determined using DC Protein Assay (Bio-Rad Laboratories, Hercules, CA, USA). For immunoprecipitation ...
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bioRxiv - Neuroscience 2021Quote: ... to a final concentration of 10 mM and shielded from light for 30 minutes.We performed an automated detergent compatible protein assay (DCA) (Bio-Rad, Hercules, CA, Cat. #5000111), normalization (to 1 μg/μL ...
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bioRxiv - Cancer Biology 2020Quote: The ddPCRs assay comprised a total volume of 20 μL containing on the one hand the droplet Supermix (Bio-Rad Laboratories, Richmond, CA, USA) at final concentration of 500 nM of forward and reverse primers and 250 nM of VIC- and FAM-labeled probes and in the other hand the DNA input containing 8 µL of eluted cfDNA (mean ...
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bioRxiv - Developmental Biology 2021Quote: ... Quantification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) reference gene RNA level was performed to determine the efficiency of RNA extraction and qPCR amplification (Bio-Rad PrimePCR assay #qHsaCED0038674). SARS-CoV-2 viral loads below 40 RNA copies/mL were categorized as undetectable and set at 1.0 log10 RNA copies/mL.
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Genomics 2020Quote: ... and 250 nM TaqMan probe (Ref-HEX and assay specific-FAM dye labeled) in a 1× Bio-Rad Supermix (Bio-Rad, Hercules, CA, USA). The 20μl PCR reaction was mixed with Bio-Rad droplet generator oil and partitioned into 15,00020,000 droplets using the Bio-Rad QX-100 droplet generator (Bio-Rad) ...
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bioRxiv - Genetics 2020Quote: ... the soluble fraction was transferred to a new tube and total protein concentration was determined by DC protein assay (Bio-Rad, Hercules, CA, USA). Following quantification ...
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bioRxiv - Cancer Biology 2022Quote: ... The fluorescence intensity was calculated relative to protein concentration in each sample as assessed by the DC™ Protein Assay (BioRad, Hercules, CA, USA) according to manufacturer’s protocol.
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bioRxiv - Genomics 2022Quote: ... Protein concentration was quantified with the Quick Start Bovine Serum Albumin Bradford assay according to the manufacturer’s protocol (Bio-Rad Laboratories, Inc., Hercules, CA) and using an Eppendorf Biophotometer 6131.
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bioRxiv - Microbiology 2022Quote: Cytokine/chemokine concentration was assessed in cell culture supernatants 48 h post transfection by using immunoassays formatted on magnetic beads (Bio-Plex Pro Human Cytokine 27-plex Assay #M500KCAF0Y) (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol via Luminex 100 technology (Luminex ...
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bioRxiv - Neuroscience 2022Quote: Bilateral tissue punches were homogenized in 1% sodium dodecyl sulfate (SDS) processing buffer and protein was quantified using the Bio-Rad protein DC assay (Bio-Rad Laboratories, Berkeley, CA). For each sample ...
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bioRxiv - Microbiology 2024Quote: ... purity was checked by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a 12% polyacrylamide gel using the Bio-Rad Protein Assay (Bio-Rad, Hercules, CA, USA), according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... The AMR genotypes of transconjugant colonies were determined by polymerase chain reaction (PCR) assays on a T100™ Thermal Cycler (Bio-Rad, Hercules, CA). Reaction volumes were 25 µL and consisted of 12.5 µL of Accustart II PCR Toughmix (Quantabio ...
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bioRxiv - Physiology 2023Quote: Enzymes were extracted using a phosphate-based buffer (50 mM KH2PO4, 1 mM EDTA, 0.05 % Triton X-100) and quantified by Bradford assay (Bio-Rad #5000006, Bio-Rad, Hercules, CA, USA). Citrate synthase activity was measured spectrophotometrically as previously described106.
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bioRxiv - Biochemistry 2023Quote: ... The elutions were then concentrated on an Amicon spin column (10 kDa cutoff) and quantified by the DC assay (Bio-Rad, Hercules, CA, USA). The eluted protein was then diluted in PBS (pH 7.4) ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was used as cell extract for further experiments after determination of the protein concentration using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA).
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... from ART or ART + GYY4137 treated groups were harvested to isolate total RNA using Qiagen RNAeasy isolation kit and 200 ng of total RNA was reverse transcribed (iScript™cDNA synthesis kit, Bio-Rad). Reverse transcribed cDNA was diluted 10-fold and amplified using primers against HIV LTRs and seminested – PCR was performed using primers and probe listed in Table S6 ...
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bioRxiv - Developmental Biology 2021Quote: ... using the whole SingleShot™ Cell Lysis Kit (SingleShot™ Cell Lysis Kit, 100 x 50 µl reactions Cat# 1725080, Bio-Rad) as follows ...
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bioRxiv - Physiology 2020Quote: ... RNA of treated cells was isolated using EZ-RNA isolation kit (Biological Industries Beit Haemek, Israel) and cDNA was synthesised using iScript cDNA synthesis kit (Bio-Rad, UK). Real-time qPCR analyses were performed on a StepOnePlus™ Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Genetics 2021Quote: ... we purified total RNA using the Qiagen RNeasy Mini kit (Cat#74106) and made cDNA using iscript cDNA synthesis kit from Biorad (Cat#1725036), followed by a Taqman assay to detect AHR transcription (Hs00169233-m1 for AHR expression and Hs0442063-g1 for GAPDH ...
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bioRxiv - Biochemistry 2021Quote: RT-qPCR was performed using the 2019-nCoV RUO Kit (Integrated DNA Technologies, #10006713) in combination with the iTaq™ Universal SYBR® Green One-Step Kit (BioRad). Each 20 μl reaction contained 10 μl 2X iTaq universal SYBR® Green reaction mix ...
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bioRxiv - Evolutionary Biology 2021Quote: RNA was isolated from cells using the Qiagen RNeasy Mini Kit (Cat# 74104) and cDNA was prepared using the iScript cDNA Synthesis Kit (Biorad, Cat# 1708890). Assays were done in triplicate using Express SYBR GreenER master mix (Invitrogen ...
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Single-cell analysis of shared signatures and transcriptional diversity during zebrafish developmentbioRxiv - Developmental Biology 2023Quote: ... Whole-larvae cDNA was generated by isolating total RNA from 3–5 dpf zebrafish larvae using the E.Z.N.A Total RNA kit (Omega, Bio-Tek INC, Cat# R6834-01) and reverse transcribing using the iScript cDNA synthesis kit (BioRad, Cat# 1708891). Fragments of coding sequences of these genes were amplified by PCR using gene-specific primers as follows:
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bioRxiv - Bioengineering 2024Quote: ... RNA was extracted using a total RNA purification kit (NORGEN, Auburn, WA) followed by cDNA synthesis utilizing iScript cDNA kit (BIORAD, Hercules, CA). Quantitative real time-PCR using iTaq Universal SYBR GREEN Supermix (BIORAD ...
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bioRxiv - Cancer Biology 2021Quote: ... a DNA extraction kit (InstaGene Matrix-Bio-Rad – USA) is utilize zed for this purpose ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized using iScript cDNA Synthesis Kit (BioRad). qPCR was performed on a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Cell Biology 2019Quote: ... before reverse transcription using iScript cDNA Synthesis kit (BioRad). Real-time PCR was performed with diluted cDNAs in a 15 μL reaction volume using Kapa SYBR Fast qPCR mix in duplicates and analyzed using the CFX96 Touch Real-Time PCR Detection System and computing ΔΔCq using the CFX96 software ...
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bioRxiv - Cell Biology 2020Quote: ... Membranes were developed with an ECL reagent kit (BioRad). Western blots were quantified using Fiji software (“Gels” command).
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bioRxiv - Genomics 2021Quote: ... whereas a KRAS mutation screening kit (Biorad, Hercules, CA) was applied to patient M10 ...
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bioRxiv - Developmental Biology 2020Quote: ... or with the iScript cDNA Synthesis Kit (Bio-Rad). Real-time PCR was performed using SYBR Green (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... The iScript Reverse Transcription Supermix kit (Bio-Rad; 1708841) was used to synthesize cDNAs from 200 ng of RNA according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: The iTaq Universal Probes One-Step kit (Bio-Rad) was used to perform duplex assays probing for the HCV genome (NS5B-FW primer ...