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Citations for Bio-Rad :
3751 - 3800 of 4242 citations for 6 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... and fractionated into 12 fractions (1ml each) using a Beckman fraction recovery system connected to an EM-1 UV monitor (Bio-Rad).
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bioRxiv - Plant Biology 2021Quote: ... and cDNA synthesized by reverse transcription of 1 µg of isolated total RNA using the iScriptTM Reverse Transcription Supermix (Bio-Rad) in a 20 µl reaction volume ...
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bioRxiv - Microbiology 2020Quote: ... membranes were subject to sequential incubation with anti-FLAG mouse antibody (1: 2000 dilution) and secondary anti-mouse IgG conjugated to HRP (Bio-Rad). After washing three times with TBST buffer ...
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bioRxiv - Microbiology 2020Quote: ... baumannii cells using a Bio-Rad GenePulser II (1.8 kV, 25 μF, and 200 Ω) in a 1 mm electrode gap cuvette (Bio-Rad). Cells were recovered by resuspension in 1 mL of SOC medium and incubated at 37°C with shaking (200 rpm ...
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bioRxiv - Pathology 2021Quote: ... The sections were then incubated in blocking buffer (10% goat serum; 1 hour) followed by incubation with either rat anti-F4/80 antibody (Bio-Rad) diluted 1:500 or rat anti-CD68 antibody (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Washed cells were embedded in 2 % Low melt agarose plugs and cells were lysed (1 hr at 37°C) with lysozyme enzyme (100 μl) using CHEF Bacterial Genomic DNA Plug Kit (Bio-Rad). DNA containing plugs were washed twice with nuclease free water then treated with Proteinase K (Qiagen ...
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bioRxiv - Microbiology 2020Quote: ... Each cDNA sample was diluted 1:15 and used directly for qPCR analysis using specific primers and the iTaq Universal SYBR green mastermix (Bio-Rad). Primers for qPCR were designed using the Primer3 software44 ...
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bioRxiv - Neuroscience 2021Quote: ... ChAT) for 5 minutes or ECL Standard (β -actin) for 1 minute and then imaged using the ChemiDocMP Imaging System (BioRad). One sample from the Vehicle group was excluded from analysis for the BDNF western blot due to a transfer bubble over the band of interest for that sample ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Macrosep and Microsep Advance centrifugal devices 3 kDa MWCO and 0.2 μm syringe filters were purchased from Pall Corporation ...
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bioRxiv - Microbiology 2021Quote: ... or wet transferred using 0.5 M sodium phosphate transfer buffer (at a constant 1 Amp for an hour using a Bio-Rad apparatus). Membranes were blocked in 5% milk in PBS-T and probed with primary antibody (overnight at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... Blots were incubated with the appropriate secondary antibodies for 1 h at room temperature and visualized using the Chemi-Doc XRS+ system (Bio-Rad). Primary antibodies used were anti-BCL-XL (abcam ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantitated by NanoDrop and 1 µg of total RNA was then reverse-transcribed to cDNA using the iScript cDNA synthesis kit (Biorad; #1708891) (Roche) ...
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bioRxiv - Neuroscience 2022Quote: ... Shooting: Dil-labeled bullets were shot through a 3.0 μm mesh 1 using a Helios Gene Gun system (Biorad, München, Germany) at 130 psi helium gas pressure.
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bioRxiv - Microbiology 2022Quote: ... added to a 15 μL reaction mixture containing 500 nM primers (sequences in Table 1) and iTaq Universal SYBR Green Supermix (Biorad, # 1725124) using a thermocycler (Mastercycler ep realplex2 ...
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bioRxiv - Evolutionary Biology 2022Quote: Plasmids pMAL-c4X-RecA and pMAL-c4X were electroporated with 1 pulse of 2.5 kV using a MicroPulse electroporator (Bio-rad, USA) into electrocompetent Lambda-lysogenized E ...
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bioRxiv - Genetics 2022Quote: ... The conidia were electroporated using 1 mm gap cuvettes (BIO-RAD Gene Pulser ®/ MicroPulser™ Cuvette) (Bio-rad, Hercules, California) at 1.5 kV ...
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bioRxiv - Genetics 2022Quote: ... Blots with HRP-linked secondary antibodies were additionally incubated for 1-5 minutes with Clarity or Clarity Max ECL Western Blotting substrates (Bio-Rad). Blots were then imaged on a ChemiDoc MP Imaging System and analyzed with Bio-Rad Image Lab software (v5.2.1).
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bioRxiv - Immunology 2022Quote: ... Yield of RNA yield was quantitated by nano-drop and 1 µg of RNA was use to reverse-transcribed to cDNA using the iScript cDNA synthesis kit (BIORAD; #1708891) (Roche) ...
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bioRxiv - Microbiology 2022Quote: ... membranes were incubated at 37°C for 1 h with goat anti-mouse IgG StarBright Blue 520 or anti-rabbit IgG Starbright Blue 700 (Bio-Rad) secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 h at room temperature ...
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bioRxiv - Plant Biology 2019Quote: ... was used at a dilution of 1:1.000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:10.000) followed by a chemiluminescence reaction (Clarity ™ Western ECL Substrate, BIO-RAD).
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bioRxiv - Genetics 2019Quote: ... blocked in 5% non-fat dry milk, primary blotting using mouse anti-mNeonGreen (1:1000, Chromtek 32F6) or hFAB™ Rhodamine Anti-Actin (12004164 BioRad), and secondary blotting using 1:3000 anti-mouse HRP (NXA931 ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Labeled EV and cells treated or not with these EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. Samples were then processed and analyzed using nano-LC-MS/MS ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: Cells or EV were lysed in PBS containing 1% SDS and protein concentration was determined using the DC™ Protein Assay from Biorad. 2μg of proteins were electrophoresed on SDS-PAGE ...
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bioRxiv - Molecular Biology 2020Quote: ... 1:1000)The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1hr and the detection was performed using the immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Products were separated by electrophoresis on a 0.7% agarose gel run in 1× TAE buffer at 55 V in the cold room and imaged on a ChemiDoc Touch instrument (Bio-Rad).
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Developmental Biology 2020Quote: ... USA) and 1 μg of RNA was reverse transcribed by using 5X iScript Reverse Transcription Supermix (Bio-Rad, Mississauga, ON, Canada) according to the manufacturer’s instructions.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Plaques of FCoV were stained with specific antibodies (primary antibody: mouse anti feline coronavirus monoclonal antibody at 1:500 dilution solution, Bio-Rad; secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... and cDNA was reversely transcribed with 0.5–1 μg RNA using the iScript cDNA synthesis kit as described in the kit (Biorad, Hercules, CA). The gene expression of Il-6 ...
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bioRxiv - Molecular Biology 2021Quote: ... The formaldehyde crosslinks were reversed by heating the samples for 1 h at 70 °C and RNA was extracted using PureZOL™ (BioRAD) as described in the RNA extraction section ...
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bioRxiv - Microbiology 2019Quote: ... Expression of AcrIIA1 mutants was confirmed by harvesting 1 OD600 unit of cells and resuspending in 200 µl 1X Laemmli Sample Buffer (Bio-Rad) followed by SDS-PAGE and immunoblotting as described above ...
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bioRxiv - Microbiology 2020Quote: The quality of genomic DNA and the PCR products were checked on 1% (w/v) agarose gel made by dissolving 1.5 g of agarose (Bio-Rad, SA) in 150 ml of 1X Tris-acetate-ethylenediaminetetraacetate (TAE ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were identified via 1% agarose gel electrophoresis and visualized in a Gel Doc system (BioRad, Hercules, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... A total of 100μL competent cells were incubated in a 1mm cuvette with 1–8μg DNA for 20 min on ice prior to pulsing in a GenePulser Xcell™ electroporator (Bio-Rad) with time constant and voltage settings at 5 ms and 1200V ...
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bioRxiv - Genetics 2020Quote: ... After four washing steps with PBST the respective secondary antibodies were added for 1 h at RT: goat anti-mouse-HRP conjugate (Bio-Rad) at 1:3000 ...
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bioRxiv - Immunology 2020Quote: ... first in 10% red blood cell (RBC) lysis buffer (Serotec, UK) for PBMCs or 1× Erythrolyse RBC Lysing Buffer (Biorad, UK) for whole blood ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated using 1 µg total RNA and random hexamers with SuperScriptTM III Reverse Transcriptase and qPCR performed with iQTM Supermix (Bio-Rad) using a CFX96 Real-Time Thermocycler (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... The indexed amplicons were mixed based in relative fluorescence units and run on a 1% agarose gel with Sybr Safe and gel extracted using a freeze and squeeze column (Bio-Rad). The product was quantified using KAPA Library Quant kit (KAPA Biosystems).
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bioRxiv - Genetics 2020Quote: ... membranes were washed in × 1 Tris-buffered saline-Tween (TSBT) and then blocked for ~10 min in 5% blocking buffer (Bio-Rad) in 1 × TBST ...
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with a secondary antibody and 1 × TBST washes the membrane was coated with chemiluminescent substrate (Clarity Max ECL substrate, Bio-Rad) and imaged on Chemidoc MP (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... 1 μL diluted cDNA (5 ng) in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each experimental sample (four source colonies ...
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bioRxiv - Biochemistry 2021Quote: ... cDNA was diluted in water in a 1:10 ratio and 1µl was used for RT-PCR was performed with an iCycler (Biorad, www.bio-rad.com/) using a Green MasterMix (Jena Bioscience ...
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bioRxiv - Physiology 2021Quote: ... cDNA was diluted 1:5 and then 2μl was added to 5ul SYBR green PCR mixture (BioRad, 1725271, Hercules, CA, USA), 2.4ul water and 1.25 pmol primer mix ...
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bioRxiv - Microbiology 2020Quote: ... mouse anti–PAI-1 antibody was coupled to carboxylated polystyrene beads (Luminex) using a Bio-Plex amine coupling kit (Bio-Rad). The coupled beads were then incubated with samples and bound PAI-1/tPA complexes detected using biotinylated rabbit anti-tPA antibody (Molecular Innovations ...
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bioRxiv - Biochemistry 2021Quote: ... both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate) (#1706515, Bio-Rad) at a concentration of 1:8000 at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μL of precleared samples were added to 12 μL of beads in PBS-casein (PBS containing 1% casein, Bio-rad Laboratories ...