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Citations for Bio-Rad :
3701 - 3750 of 4242 citations for 6 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: ... 39) and 1 µg of total RNA was reverse transcribed to cDNA using the iScript Select cDNA Synthesis Kit (Bio-Rad) with random hexamers ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs were performed using 2ul of 1:100 diluted first PCRs as a template with qPCR primers (Table S4) and iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... A one in 10 dilution of cDNA was used for qPCR reaction mixture with final concentration of 1× SsoAdvanced universal SYBR super mix (Bio-Rad) and 1×PrimePCR assays for the designated target ...
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bioRxiv - Neuroscience 2021Quote: ... of each sample was loaded on an 8-16% (for TSPO, 3b-HSD, and StAR) or 4-20% (for mERα, caveolin-1, and PKA) SDS precast gels (Bio-Rad) and separated by electrophoresis ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were then incubated with secondary antibodies (1:10000, goat anti-rabbit or goat anti-mouse IgG HRP conjugate (Bio-Rad) for 1 hour and detection was performed using the Immobilon-luminol reagent assay (EMP Millipore).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Neuroscience 2021Quote: ... for 10 minutes followed by 15 minutes of tris-buffered saline with 0.25% Triton X-100 then blocked for 1 hour in tris-buffered saline with Tween 20 solution at 0.05% (BioRad #161-0781) supplemented with sterile 2% Normal Donkey serum (Abcam #ab138579 ...
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bioRxiv - Biochemistry 2021Quote: ... The melting curves were measured at 1°C/min at the FRET channel in triplicate measurements and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... Western blot was performed using 8 or 10% mini gels followed by wet transfer (1 h, 100 V) to Western grade nitrocellulose (Bio-Rad). Blots were incubated in Intercept blocking buffer (LI-COR ...
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bioRxiv - Biochemistry 2021Quote: ... The cells were replenished with compounds every 24 h and counted on days 1-9 using automated cell counter (Bio-Rad). KYSE-520 cells were plated onto 96-well plates (500 cells per well ...
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bioRxiv - Neuroscience 2021Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Neuroscience 2021Quote: ... USA) that were diluted 1/5000 in TBS-T and revealed by enhanced chemiluminescence plus (ECL) solution (Biorad Laboratories, ON, Canada). β-actin was used to ensure equal protein loading ...
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bioRxiv - Cancer Biology 2021Quote: ... were run on a 1 mm thick 10% SDS-PAGE gel and stained with CBB G-250 staining solution (Bio-Rad) at RT for 1.5 h ...
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bioRxiv - Cancer Biology 2020Quote: ... Revelation was performed using secondary antibodies conjugated to horse-radish peroxidase (GE-Healthcare) diluted 1:2,000 in saturation solution and Opti-4CN™ colorimetric kit (Bio-Rad). ImageJ software was used to measure the relative intensity of protein bands.
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bioRxiv - Cell Biology 2021Quote: ... Reverse transcription of 1 μg of RNA in a total volume of 20 μl was performed with iScript cDNA Synthesis Kit (Bio-Rad) prior to real time PCR (iCycler ...
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bioRxiv - Physiology 2020Quote: ... We synthesized a total of 500 ng (pituitary) and 1 μg (gonad) of RNA into cDNA using the iScript cDNA synthesis kit (Bio-Rad) in 20 μl reactions according to manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... Electrophoresis of PCR products was done on EtBr-stained 1% agarose gels and photographed on a Gel Doc XR instrument (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... The melting curves were measured at 1°C/min at the FRET channel and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody ALP conjugate (1:40,000 dilution) was also used and its blots were analysed on Gel doc system (BioRad, CA, USA).
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bioRxiv - Plant Biology 2021Quote: ... benthamiana leaves was infiltrated with 1 mM luciferin 40 h after inoculation of the Agrobacteria and then imaged under the Gel Doc XR+ System (Bio-Rad) with the Bolt mode ...
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bioRxiv - Synthetic Biology 2021Quote: ... We performed cDNA synthesis with 0.5-1 μg of total RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed Real Time quantitative PCR (RT-qPCR ...
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bioRxiv - Microbiology 2020Quote: ... RNA purity and integrity was verified by 1% (w/v) agarose gel electrophoresis in an Experion Automated Electrophoresis System (Bio-Rad). One µg of RNA was reverse-transcribed into cDNA (iScript Kit ...
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bioRxiv - Microbiology 2020Quote: ... or anti-PfADF1 (1:2000, (Wong et al, 2011)) and HRP-coupled goat anti-mouse or -rabbit secondary antibody (1:5000, STAR120P/STAR121P, Bio-Rad). sBlots were washed in PBST and detected using ECL reagent (Amersham ...
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bioRxiv - Systems Biology 2020Quote: ... and used 500 ng - 1 ug RNA to make cDNA using the iScript cDNA Synthesis Kit (Bio-Rad Laboratories, Hercules, CA). 0.1 of cDNA (i.e ...
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bioRxiv - Microbiology 2020Quote: ... 0.25 U Taq DNA polymerase and 1 µL of DNA template (5-10 ng/µL) on a T100 TM Thermal Cycler (Bio-Rad). Thermal cycling conditions for bacteria and archaea were as follows ...
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bioRxiv - Plant Biology 2021Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α and PP2A as housekeeping genes ...
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bioRxiv - Plant Biology 2021Quote: ... ∼1 ug of RNA from resulting extraction was used as template in standard cDNA synthesis reaction (BioRad iScript cDNA Synthesis Kit). 200 ng of resulting cDNA was used in qRT-PCR reactions to quantify POL expression levels (PowerUp SYBR Green 5x Master Mix-Thermo) ...
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bioRxiv - Plant Biology 2021Quote: ... All qPCR reactions were run for 94°C for 2 min followed by 94°C for 15 s and 60°C for 1 min in a BioRad CFX384 Real Time System (Biorad, USA) qPCR machine ...
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bioRxiv - Microbiology 2020Quote: ... Clarified lysates were incubated with 2 ml of glutathione resin and rotated for 1 h at 4°C in a 25 ml disposable drip column (Bio-Rad). Settled resin was washed with 25 column volumes (CV ...
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bioRxiv - Microbiology 2021Quote: ... and revelation with a IRDye 800CW Goat anti-mouse IgG secondary antibody (dilution 1/10000, Li-Cor Biosciences) using a ChemiDoc− Touch Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Then the samples were treated at 60oC for 1 h and then enzyme deactivated 95oC for 15 min in a thermocycler (Bio-rad). After the treatment ...
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bioRxiv - Neuroscience 2020Quote: ... the membranes were further incubated in secondary antibody solutions (TBST with 5% Non-Fat Dry Skinned Milk and Horseradish Peroxidase conjugated secondary antibodies Goat Anti-Rabbit/Mouse, 1:10000, Bio-Rad) for 2 hours at room temperature ...
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bioRxiv - Bioengineering 2020Quote: Organoids fixed in 4% w/v PFA and immunostained were embedded in 1% w/v low melting agarose (Bio-Rad Laboratories) solution and placed in an Eppendorf tube ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were subsequently centrifuged at 1200 rpm for 5 minutes before their pellets being resuspended in 1 ml medium for cell counting using TC10 automated cell counter (BioRad USA) before re-plating ...
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bioRxiv - Cell Biology 2022Quote: ... Western blotting was performed according to the manufacturer’s instructions with minor modifications (SDS-PAGE run at 110 V, transfer onto PVDF membrane for 1 h at 110 V (BIO-RAD)) ...
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bioRxiv - Physiology 2022Quote: ... pH was brought back to neutral with 1 µL of TRIS buffer pH9 and samples were denatured in Laemmli buffer (Bio-Rad) at 95°C for 5 minutes and loaded for SDS-PAGE.
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bioRxiv - Genetics 2022Quote: Template cDNA pools were generated from 1 µg RNA using cells grown under standard glucose conditions (as above) by using iScript (Bio-Rad) RT with first-strand synthesis priming from a mixture of random hexamers and oligo-dT ...
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bioRxiv - Biochemistry 2022Quote: ... The samples were separated on a pre-heated 45 cm sequencing gel (45 °C, 65 W, 1 h) and visualized using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... and the primers for murine IFNAR 1 and beta-Actin in a MyiQ™ Single-Color Real-Time PCR System (BioRad). Products of RT-PCR were separated by electrophoresis on a 2.5% agarose gel in 1x TBE buffer ...
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bioRxiv - Plant Biology 2022Quote: ... RNA electrophoresis was performed at 200 V for 1 h and then RNA was transferred to a nylon membrane using MiniProtean 3 system (BioRad, USA). Transfer conditions were 100 V for 1 h at 4 °C in TBE buffer 1 × ...
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bioRxiv - Microbiology 2022Quote: ... Beads were washed 3 times in PBS and eluted in 40 μL 2X Laemmli Buffer with 1:20 β-mercapto-ethanol (BioRad) at 95°C for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... All PCR reactions were run in triplicate using 1 μL of cDNA in a 25 μL final volume with iQ SYBR Green Supermix (Bio-Rad) and 200 nM each of forward and reverse primers (see Supplementary Table 5) ...
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bioRxiv - Bioengineering 2022Quote: ... All the eluted fractions were tested using a Bradford reagent assay (diluted 1:5 with ddH2O) (190 μL Bradford, 10 μL sample) (Bio-rad). Positive fractions were desalted using 100 kDa amicons (Merck ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were incubated with primary and HRP-secondary antibodies in TBST buffer with 5% milk (RT for 1 hour or overnight at 4 °C) and revealed with an ECL solution (BIO-Rad) following manufacturer’s instructions.
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bioRxiv - Neuroscience 2020Quote: ... 7.5 μl of SsoAdvanced Universal SYBR Green Supermix and 1 μl of the following DNA templates (Prime PCR Assay, Bio-Rad): mt-Co2 (mitochondrial ...
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bioRxiv - Plant Biology 2021Quote: ... The blot was washed 3 times with TBST and incubated for 30 min in 1% milk prepared in TBST with anti-mouse HRP conjugate (Bio-Rad). Chemiluminescent detection was performed using SuperSignal West Dura Extended Duration (Thermo ...
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bioRxiv - Immunology 2020Quote: Total RNA (up to 1 µg per reaction) from BMDM was reverse transcribed with iScript cDNA synthesis kit (Bio-Rad, USA). Quantitative PCR was performed using 1 µl of cDNA ...
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bioRxiv - Developmental Biology 2021Quote: ... and 25 µg of the targeting vector was transfected into 1 × 107 of Nmt1-heterozygous cells11 by electroporation (240 V, 500 µF) with Gene Pulser II (Bio-Rad). One week later ...