Labshake search
Citations for Bio-Rad :
3651 - 3700 of 4242 citations for 6 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
-
bioRxiv - Cell Biology 2024Quote: ... blots were incubated in 1-shot ECL solution (Kindle Biosciences LLC) and imaged with a ChemiDoc MP Imaging System (BioRad).
-
bioRxiv - Genetics 2024Quote: ... followed by purification and size selection on a 1% agarose gel with 0.6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
-
bioRxiv - Plant Biology 2024Quote: ... cDNA was then synthesized from 1 µg total RNA (measured by spectrophotometer) with the iScriptTM cDNA synthesis kit (Bio-RAD) according to the company’s instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... The HRP-coupled secondary antibodies were used at a 1:10,000 dilution followed by development using ECL substrate from Bio-Rad Laboratories ...
-
bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR was carried out using the PerfeCTa SYBR Green Fastmix (Quantabio, 95071) and gene-specific primers (Table 1) on CFX96 system (Biorad). Relative gene expression was determined using the ΔΔCt method.
-
bioRxiv - Neuroscience 2024Quote: ... Membranes were stained with Ponceau S to visualize protein loading and VAChT was revealed with rabbit anti-VAChT (1:1000, SYSY, 139103) by ECL procedure (Clarity Western ECL, Biorad) on a chemiluminescence imager (ChemiDoc ...
-
bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated with secondary antibody diluted 1:7500 in blocking buffer (anti-rabbit, anti-mouse HRP, Bio-Rad). Signal was detected using Pierce ECL Western Blotting Substrate (Fisher 32106) ...
-
bioRxiv - Neuroscience 2024Quote: ... Brain sections were then incubated in primary antibodies at 4°C overnight (1:500, rat anti-mouse CD68, Bio-rad Cat ...
-
bioRxiv - Bioengineering 2024Quote: ... and polystyrene disposable cuvettes (Semi-Micro, 1 cm path, 1.5 mL, 12.5 mm square x 45 mm high; Bio-Rad, 2239955). The alginate and CaSO4 were mixed ...
-
bioRxiv - Biochemistry 2024Quote: ... 50 µL of the cell-oligonucleotide mixture was transferred to a pre-chilled 1 mm electroporation cuvette (BioRad catalog #1652083) and transformed with 1.8 kV ...
-
bioRxiv - Neuroscience 2024Quote: ... Proteins were electrotransferred onto nitrocellulose membranes using the trans-blot turbo transfer system (constant voltage: 25V, maximum amperage: 1 A; 30 min, Biorad) and membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
-
bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated secondary antibodies were used at 1:5000 dilutions and chemiluminescence signal was produced using the Clarity western ECL substrate (BioRad) and detected using a ChemiDoc imaging system (BioRad) ...
-
bioRxiv - Cell Biology 2024Quote: ... cDNA samples were obtained from 1 µg RNA using the iScriptTM cDNA synthesis kit (Bio-Rad, Cat no. 170-8891) according to the manufacturer’s instructions ...
-
bioRxiv - Systems Biology 2024Quote: ... 1 µg of total RNA were processed using the iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; www.bio-rad.com), to achieve genomic DNA digestion and first-strand cDNA synthesis ...
-
bioRxiv - Molecular Biology 2024Quote: ... 60 mM EDTA pH 7.0 containing 5% β-mercaptoethanol and 1 mg/ml zymolyase 100T) at 40 °C and aliquoted to plug molds (BioRad) to prepare 20 agarose plugs ...
-
bioRxiv - Molecular Biology 2024Quote: ... The reaction was resolved by electrophoresis on a 6% non-denaturing polyacrylamide gel in TBE (100 mM Tris, 90 mM boric acid, 1 mM EDTA) buffer and visualised by ChemiDoc (BioRad) using SYBR Gold stain or imaging in the 6FAM ...
-
bioRxiv - Biochemistry 2024Quote: ... We loaded 10 µL of proteins at 10 µM mixed in a 3:1 ratio with 4X Laemmli Buffer Sample (Biorad) provided with 10% DTT ...
-
bioRxiv - Biophysics 2021Quote: To extract lipids, purified SaPgsA (5.82 mg ml-1, 200 μl) was mixed with 40 mg SM2 Biobeads (Bio-rad, USA) and gently shaking overnight at 4 °C to remove detergent ...
-
bioRxiv - Cancer Biology 2021Quote: ... 0.2–2.2 Mb, S. cerevisiae Ladder: Bio-Rad, 1703605; CHEF DNA Size Marker, 1–3.1 Mb, H. wingei Ladder: Bio-Rad, 1703667) and PFGE was performed using the CHEF Mapper XA System (Bio-Rad ...
-
bioRxiv - Cancer Biology 2021Quote: ... For immunohistochemistry (IHC) experiments following primary antibodies were used: IgG anti-human CD3 antibody (1:200, clone CD3-12, Bio-Rad) and anti-PCNA antibody (1:1000 ...
-
bioRxiv - Cell Biology 2020Quote: ... membranes were incubated with StarBright Blue 700 Goat Anti-Mouse IgG secondary antibody (1:2500, Bio-Rad, Cat# 12004159, RRID: AB_2884948), StarBright Blue 520 Goat Anti-Rabbit IgG secondary antibody (1:2500 ...
-
bioRxiv - Developmental Biology 2020Quote: ... Stain-Free gels were activated with UV light for 1 minute and imaged for total protein on gel using a ChemiDoc MP imager (Bio-Rad). Proteins were transferred to PVDF blotting membranes (Bio-Rad ...
-
bioRxiv - Cell Biology 2019Quote: ... we used 1/20th volume of a cDNA reaction to program a PCR reaction using iQ SYBR Green Supermix (Bio-Rad). PCRs were amplified on a CFX96 Real-Time System (Bio-Rad ...
-
bioRxiv - Cell Biology 2019Quote: ... IL-10 and MCP-1 in cell culture supernatants at 24 and 96 h post-infection on the Bioplex 200 (Bio-Rad). The Bioplex Manager 6.1 software was used for data analysis (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
-
bioRxiv - Cell Biology 2020Quote: ... The product yield for each condition was estimated by resolving 10% of the reaction on a 1% EtBr-agarose gel and quantifying the band intensity of the resulting amplicon with ImageLab software (Bio-Rad). The 1.6-kb amplicon was gel-extracted using the QiaQuick Gel Extraction Kit (Qiagen ...
-
bioRxiv - Cell Biology 2020Quote: ... Signals were developed by incubating with 1/5000 fold dilution of anti-rabbit or anti-mouse secondary antibodies for 1 h at room temperature and quantified using the Image Lab Software on a ChemiDoc XRS system (Bio-Rad).
-
bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed with 1 μg total RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad 170-8841). cDNA equivalent to 20 ng of total RNA was then amplified in duplicate using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 172-5274 ...
-
bioRxiv - Cell Biology 2020Quote: ... They were immediately transformed by bombardment with plasmid-coated 1-μm gold particles with a helium-driven particle accelerator (PDS-1000/He; Bio-Rad) using 1350 psi rupture disks and a vacuum of 28 inches of mercury ...
-
bioRxiv - Immunology 2021Quote: ... Slides were incubated overnight at 4 °C with the following primary antibodies (1:100, in blocking buffer): mouse anti-human MBP (BMK-13, antihuman MBP (BMK-13, MCA5751 Bio-RAD), rabbit anti-human PD-L1 (E1L3N ...
-
bioRxiv - Developmental Biology 2021Quote: ... and OPN) and control (HPRT) transcripts were amplified (Supplemental Table 1) using SsoAdvanced Universal SYBR Green Supermix (Biorad, Hercules, CA, USA). Established TaqMan assays were used to determine the expression of Lgr6 and TBP intra-well controls ...
-
bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by electrophoresis at 70V for 15 minutes followed by 100V for approximately 1 hour using 10x Tris/Glycine/SDS running buffer (BioRad, 1610772).
-
bioRxiv - Neuroscience 2021Quote: ... analysis was performed through reverse transcription on 0.5-1 μg of total RNA using the iScript cDNA synthesis kit according to the manufacturer’s instructions (Bio-Rad, Hercules, CA). The cDNA was diluted 1:20 in nuclease-free water ...
-
bioRxiv - Microbiology 2019Quote: ... The copy number of a host gene RPP30 was determined using 1 μL of HEX-labeled PrimePCR RPP30 (Bio-Rad, 10031243) in the reaction to calibrate the amount of DNA input ...
-
bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
-
bioRxiv - Molecular Biology 2020Quote: ... Samples were heated to 50°C for 1-10 min before addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexing vigorously for 5 s before pipetting in plug mould (Bio-Rad 1703713 ...
-
bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
-
bioRxiv - Genomics 2020Quote: ... The indexed amplicons were mixed based in relative fluorescence units and run on a 1% agarose gel with Sybr Safe and gel extracted using a freeze and squeeze column (Bio-Rad). The product was quantified using Kapa Illumina Quant kit.
-
Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... supplemented with 1 mM DTT for reduction of disulfide bonds and protein concentration was determined by Quick-start Bradford assay (Bio-Rad). Proteins were alkylated with 5.5 mM chloracetamide (45 min ...
-
bioRxiv - Cancer Biology 2019Quote: ... cDNA was produced from 1 μg of purified RNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories). qPCR reactions were performed with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories ...
-
bioRxiv - Cancer Biology 2019Quote: ... secondary antibodies were then incubated for 1 hour at room temperature followed by detection of bands by chemiluminescence in ChemiDoc XRS+ (Bio-Rad).
-
bioRxiv - Neuroscience 2019Quote: ... 0.02% NaN3 and 1 mM DTT at pH 7.4 at 37°C in a thermal cycler (BioRad DNA Engine PTC-200). In some cases ...
-
bioRxiv - Immunology 2020Quote: ... A 1 µg sample of total RNA was reverse transcribed to complementary DNA using the iScript cDNA Synthesis Kit (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2019Quote: ... Alternatively broken cyst walls either before or after digestion with trypsin were reconstituted in 1× reducing SDS/PAGE loading buffer and run on a 4–20% precast polyacrylamide TGX gel (Bio-Rad). Bands stained by colloidal Coomassie blue were excised and washed with 50 mM NH4HCO3/acetonitrile (ACN) ...
-
bioRxiv - Genomics 2019Quote: Differentiating and IFC-305 treated HepaRG cells cultured for 1 week were trypsinized and counted using TC20 automated cell counter (Bio-Rad) with dual chamber slides and trypan blue (Bio-Rad 1450003) ...
-
bioRxiv - Neuroscience 2019Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
-
bioRxiv - Zoology 2019Quote: ... 0.5 ml of the heptane phase were mixed with ethanol in a 1:3 ratio (v/v) and the absorbance at 233 nm was measured on a SmartSpec Plus spectrophotometer (Bio-Rad) with extraction blanks used as references ...
-
bioRxiv - Physiology 2019Quote: ... Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad) according to the manufacturer’s instructions (Clontech ...
-
bioRxiv - Cancer Biology 2020Quote: ... Aqueous droplets with a 20-µl volume containing final concentrations of 1× Droplet Digital PCR Supermix for probes (Bio-Rad Laboratories) and 100 ng of the genomic DNA template were generated ...