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Citations for Bio-Rad :
3901 - 3950 of 4242 citations for 6 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... we electroporated a small fraction of the assembly reaction into inhouse DH10B cells using 1 mm cuvettes and a micropulser (Bio-Rad) set at 1.8 kV ...
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bioRxiv - Microbiology 2023Quote: ... Amplicons were visualized in 1% agarose gels stained with ethidium bromide using a ChemiDoc Imaging System (Bio-Rad Laboratories, Hercules, CA). We then sent amplicons to the Génome Québec Innovation Centre (McGill University ...
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bioRxiv - Molecular Biology 2023Quote: RT-qPCR was employed to find the levels of quantification of mucin 1 and 4 mRNA transcripts according to protocol of the Real-Time PCR system (BioRad./ USA)[39] ...
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bioRxiv - Cell Biology 2023Quote: ... diluted to 1 ng/μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Bioengineering 2023Quote: ... followed by goat anti-mouse IgG (H+L)-horseradish peroxidase (HRP)-linked secondary antibody (1:1000, 1721011, Bio-Rad, RRID: AB_11125936). Detection of the horseradish peroxidase signal was carried out using Clarity Western enhanced chemiluminescence (ECL ...
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bioRxiv - Plant Biology 2023Quote: Lysate was filtered and applied to a 1 mL Mini Profinity IMAC cartridge using BioLogic low-pressure chromatography system (Bio-Rad). An imidazole gradient was used to elute the protein in elution buffer (300 mM imidazole ...
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bioRxiv - Biochemistry 2023Quote: ... The samples were then spun for 1 min at 20k x g and run on a 10% SDS gel before transferring to nitrocellulose membranes (Bio-Rad) and blocking as described above ...
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bioRxiv - Bioengineering 2022Quote: ... After transferring the proteins to nitrocellulose membrane using a transfer buffer (25 mM Tris/192 mM glycine/0.1 (w/v) SDS/20% MeOH) for 1 h at 150 mA in a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad), the membrane was saturated for 2 h at RT with 5% semi-skimmed milk in PBS-Tween (0.1% ...
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bioRxiv - Bioengineering 2023Quote: ... Equal amounts of the proteins (50 µg) were electrophoresed (125 volts, 1 h) on a 4-20% Mini protean TGX stain-free protein gel (Bio-Rad) and transferred onto nitrocellulose membranes (125 volts ...
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bioRxiv - Bioengineering 2023Quote: ... Slides were then washed with 1x TBS before blocking in a 1% bovine serum albumin (5000206; Bio-Rad, Hercules, CA, USA), and either 5% donkey (D9663-10ML ...
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bioRxiv - Bioengineering 2023Quote: ... 1 µL of the formed cDNA was used in 10 µL reactions using the SsoAdvanced universal SYBR green supermix (Bio-rad) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... samples were transferred onto PVDF membranes (activated in methanol for 1 minute) using the Trans-Blot® TurboTM Transfer System (BioRad). Transferred membranes were washed in tris-buffered saline (TBS ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Molecular Biology 2023Quote: ... cDNA was then diluted 1:100 and 1.5 µL was use per 15 µL reaction with iTaq Universal SYBR Green Supermix (Bio-Rad # 1725122) and 250 nM primers (final ...
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bioRxiv - Biochemistry 2023Quote: ... 20 minutes) and the clear lysate was purified on HisTrap HP 1 ml affinity column (Cytiva) by FPLC system (NGC™, BioRad). The protein was eluted from the column using a linear gradient (Elution buffer ...
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bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and treated with a lysis buffer (150mM NaCl, 50mM Tris pH7.5, 1% Triton X-100 (Bio-Rad #1610407), 1% SDS ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The membranes were then washed three times (15 min each at room temperature) ...
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
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bioRxiv - Neuroscience 2023Quote: ... 10-30 μg of protein extracts were run at 100V for 1 hr on 10% pre-cast SDS-PAGE gels (Bio-Rad), then electro-transferred to 0.2 μm nitrocellulose membranes using the Trans-Blot Turbo Transfer system (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: ... and cDNA was synthesized from 1 μg of total RNA using the Reverse Transcription system (iScriptTM cDNA synthesis kit, Bio-Rad). Quantitative Real time PCR was performed using Sybr Green Master Mix (SYBR Green master mix ...
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bioRxiv - Physiology 2023Quote: ... diluted to 1 ng/ μl) was amplified using the Phusion High-Fidelity DNA Polymerase kit on a thermocycler (T100, Bio-Rad) with the following programmes ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were then stained with Coomassie Brilliant Blue G (80 mg dissolved in 1 L water with 30 mM HCl) and destained with water before visualising using a ChemiDoc MP Imaging System (Bio-Rad). For mNeonTP-loaded encapsulins ...
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bioRxiv - Synthetic Biology 2024Quote: The samples (1 µL) were subjected to SDS-PAGE and transferred to a nitro cellulose membrane (Bio-Rad Laboratories, CA, USA). The membrane was blocked using 3% skim milk in PBST (137 mM NaCl ...
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bioRxiv - Plant Biology 2024Quote: pTRV1 and pTRV2-Del/Ros-1 harboring the SlATG7- or SlATG2-derived fragments were introduced into Agrobacterium strain GV3101 using electroporation (BIO-RAD). A 5 mL culture was grown overnight at 28°C in a medium containing kanamycin ...
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bioRxiv - Cell Biology 2024Quote: ... cDNA was synthesized using 1 µg of each total RNA preparation as template and the iScriptTM Reverse Transcription Supermix for qRT-PCR Kit (Bio-Rad). Primers listed in supplemental table S7 were employed for RT-PCR and qRT-PCR analysis of NtGEF12 transcript levels in comparison to reference gene expression ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodisc formation was initiated by adding washed Bio-Beads (200 mg dry weight per 1 mL of the protein mixture; Bio-Rad), and the mixture was rotated for two hours at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: Proteins from SDS-PAGE gels were transferred onto a PVDF (polyvinylidene difluoride) membrane (pre-activated in methanol for 1 min) using Trans-Blot Turbo transfer system (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was purified with QIAquick® PCR purification kit and 1-10 ng of immunoprecipitated or input DNA were amplified with iQ™ SYBR Green Supermix (BioRad) using a Real-Time PCR machine (BioRad IQ™ 5) ...
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bioRxiv - Bioengineering 2023Quote: ... 20 μL of PCR product was loaded and run on a 1% (weight/volume) agarose gel and imaged with the ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitated DNA and 10% of corresponding input DNA (1:10 diluted) were used for qPCR with SsoAdvanced Universal SYBR Green supermix (Bio-Rad) using CFX 96 (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Systems Biology 2023Quote: We performed cDNA synthesis with 0.5-1 μg of total purified RNA using the iScript Select cDNA Synthesis Kit (Bio-Rad Laboratories). We performed quantitative reverse transcription PCR (qRT-PCR ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were mixed 1:6 with a loading dye and run on an agarose gel (1%) in Tris-acetate-EDTA buffer supplemented with 0.2 µG/mL of Ethidium Bromide (Biorad, Hercules, California). DNA was visualized under a short-wavelength UV light to confirm the lack of amplification for Heat-Killed Microbiome PMA-treated in comparison to the Heat-Killed Microbiome control (Supplementary Figure 2).
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bioRxiv - Physiology 2024Quote: ... was used to wash the membranes before incubating with goat anti-rabbit horseradish peroxidase conjugated secondary antibody at 1:5000 dilution (1706515, Bio-Rad) for 90 min at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... 1−10 ng of sample DNA was used for qPCR using a MiniOpticon qPCR detection system (Bio-Rad Laboratories, Hercules, USA). The iQ SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of total RNA from each sample was converted to cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708841). Then qPCR reactions were set up using TaqMan® Fast Advanced Master Mix (ThermoFisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times with and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... After gel polymerization was performed pre-electrophoresis of empty gel in 5% acetic acid (running buffer) for 1-1.5h/150 V in reverse polarity of electrophoretic BioRad system (Bio-rad, USA). The running buffer was discarded ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated on 10 % polyacrylamide gels and blotted onto a nitrocellulose membrane at 100 V for 1 h using the Mini Trans-Blot system (Bio-Rad). Membranes were blocked with 5 % milk powder in PTw (1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... Each well was then blocked with 150 µL of blocking buffer consisting of 1×PBS + 4% (w/v) nonfat milk (Bio-Rad). The plates were blocked for 1 h at room temperature and then washed five times with PBST ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were applied for 1 hour at room temperature (1:5,000; CST, #7074) before detecting protein bands at the BioRad ChemiDoc MP Imaging System using chemiluminescence (BioRad, 170-5061).
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bioRxiv - Genetics 2024Quote: ... cDNA was diluted 1:4 with water and 2ul used as template for qPCR using 250nM primers using the SsoFast EvaGreen Supermix (BioRad 1725200) on an Applied Biosystems ViiA 7 Real Time PCR System ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples from the co-IP and were separated on 8% SDS-PAGE gels for 1 h at 120 V and transferred overnight at 20 V on to PVDF membranes (Bio-Rad). Blots were probed with rabbit anti-NMIIB/Myh10 (1:2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 ng template library DNA[21] in a total reaction volume of 20 µL using T100 Thermal Cycler (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Neuroscience 2024Quote: ... The gel was electrophoresed for 10 minutes at 50V then 1 hour at 120V in Tris-Glycine SDS buffer (Bio-rad). The gel and nitrocellulose membrane were then equilibrised in transfer buffer (Bio-Rad ...
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bioRxiv - Cancer Biology 2024Quote: ... was added to each sample and heated at 95 °C for 1 hour in a thermal cycler (S1000 with 384-well reaction module, Bio-Rad) set to a constant lid temperature of 110 °C ...
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bioRxiv - Biophysics 2024Quote: ... the GRB2 protein was eluted by linearly increasing the concentration of Imidazole from 20 to 500 mM with a flow rate of 1 ml/min in buffer A using Econo Gradient Pump (BIO-RAD). The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck) ...