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Citations for Bio-Rad :
3551 - 3600 of 4242 citations for 6 Bromo 2 tert butyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The membrane was washed with the washing solution twice and then reacted with 4-chloro-1-naphthol (Bio-Rad Laboratories) in the HRP color development buffer (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2021Quote: ... membrane was washed repeatedly with PBS-T and incubated 1 h at RT with the following secondary antibody diluted 1/5000 in milk PBS-T: Goat anti-rabbit IgG HRP conjugate (170-6515; BioRad). After the same washing procedure ...
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bioRxiv - Neuroscience 2021Quote: Recombinant or mouse-extracted tau fibrils were diluted in PBS as indicated in Supplementary Figure 1 and applied to 0.2 µm nitrocellulose membrane using the Bio-Dot microfiltration apparatus (Bio-Rad). The membranes were then blocked in 5% milk TBS-Tween 20 and subsequently incubated with primary antibody overnight ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were scanned the following day after 1 hour incubation with secondary antibodies using Odyssey CLx Imaging System or ImageQuant system (BioRad, HRP anti-rabbit or anti-mouse (CST ...
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bioRxiv - Immunology 2021Quote: ... were added to each well and incubated with cross-absorbed anti-mouse IgG HRP polyclonal antibody (diluted 1:10,000) (STAR117P, Bio-Rad) for 2h at RT on a Multiscan FC Plate Reader (Thermo Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... An aliquot was removed and diluted 1:10 in 1xPBS for cell counting using an automatic counter (Biorad TC-20) with counts spot checked by manual recount using a hemocytometer as needed ...
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bioRxiv - Cell Biology 2021Quote: ... PCR amplicons were resolved in 1% agarose (Melford) TBE gels containing 1x SYBRsafe and visualised on a Chemidoc MP (BioRad). A full list of oligonucleotides and vectors are presented in Table S1 ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples for qPCR were prepared with primers against target genes (Table 1) and mixed with SSO Advanced Universal SYBR Green Supermix (BioRad) in 96-well plates and analyzed on a CFX96 qPCR machine (BioRad) ...
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bioRxiv - Molecular Biology 2021Quote: ... cDNAs were diluted 1:10 and 1µl of diluted cDNA was used per qPCR reaction with iTaq Universal SYBR Green supermix (Bio-Rad) and the following pairs of primers ...
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bioRxiv - Plant Biology 2020Quote: ... cDNA was synthesized using 1 µg of total RNA and reverse-transcribed with the iScript cDNA synthesis kit (Bio-Rad). Synthesized cDNAs were then quantified by real time quantitative PCR (qPCR ...
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bioRxiv - Bioengineering 2022Quote: ... or ~7.5 million cells/mL (Fig S18c) before loading into wells of preformed phantoms consisting of 1% agarose (Bio-Rad) in PBS.
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bioRxiv - Neuroscience 2022Quote: 1 μg of total RNA purified from differentiated cells was reversed transcribed using the One-step SuperScript kit (Bio-Rad). The qRT-PCR reaction was performed in a CFX96 Real-time PCR detection system (Bio-Rad ...
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bioRxiv - Molecular Biology 2022Quote: ... The following primary antibodies were used for western-blotting at 1/1,000 dilution: anti-V5 clone SV5-Pk1 (mouse, monoclonal, BioRad, #MCA1360), anti-Rpb3 clone 1Y26 (mouse ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 μg of ssDNA were added to 95 μl of VSV-G-EVs in trehalose buffer and EVs then transferred to a 1 mm electroporation cuvette (BioRad) placed on ice ...
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bioRxiv - Biophysics 2022Quote: ... The mixture was then heated up to 95°C for 5 minutes and cooled down to 30°C at the rate of 1°C per minute using a thermal cycler (BioRad).
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bioRxiv - Biophysics 2022Quote: ... 2 μl of recombinant proteins and 1 μl of a molecular weight marker (Precision Plus Protein Kaleidoscope Prestained Protein Standards, BioRad) were applied to the PAGE lane (10 wells ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of protein (15 μg) were loaded on 4-15% 1 D polyacrylamide Mini-PROTEAN TGX Stain-Free gels (BioRad) and run in 1X Tris Glycine SDS buffer (BioRad ...
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bioRxiv - Bioengineering 2022Quote: ... droplet-digital PCR was performed for 16 extracted RNA samples using the 1-Step RT-ddPCR Advanced Kit for Probes (BioRad) and the CFX96 thermocycler (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Membranes were washed three times and incubated with secondary antibodies: Donkey anti Sheep/Goat IgG (dilution 1:100,000, #STAR88P, Bio-Rad Laboratories ...
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bioRxiv - Plant Biology 2022Quote: ... the membrane was incubated 3 h with a solution containing a secondary goat-HRP anti rabbit (1:10 000) (Biorad) (milk 0.5 % TBST) ...
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bioRxiv - Microbiology 2022Quote: ... HRP-linked Goat αRat antibody (Pierce 31470) was used at 1:30,000 and detected with an HRP Substrate kit (Bio-Rad) and Bio-Rad ChemiDoc imager ...
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bioRxiv - Neuroscience 2022Quote: ... membranes were incubated for one h at room temperature (RT) with horseradish peroxidase (HRP)-coupled secondary Abs at 1:2000 for one h (all secondary Abs from Biorad, raised in goatanti-rat HRP Cat # 5204-2504 and anti-mouse HRP Cat # 1721011) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... detergents and undigested protein were removed using a Bio-Scale Mini Macro-Prep High S 1 mL cartridge (Bio-Rad) on an Agilent 1100 HPLC system at 1 mL/min ...
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bioRxiv - Microbiology 2022Quote: ... Equivalent to 1×106 parasites were resolved by electrophoresis on a Mini-Protean TGX™ precast gel (Bio-rad, California). Lipoylated proteins were detected with rabbit anti-lipoic acid antibody (ab 58724 ...
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bioRxiv - Neuroscience 2023Quote: ... Free floating sections were blocked with 3% donkey serum and incubated overnight with rat anti-CD68 (1:200, Bio-Rad). Aβ deposition and CD68 staining were both developed with a Vectastain ABC kit and DAB reaction ...
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bioRxiv - Neuroscience 2022Quote: ... 10 µl of prepared sample containing 6.7 µl synaptosome lysate or 1-1.5 µl SV eluate was subjected to SDS-PAGE on 4-20% gradient gels (Criterion TGX, Bio-Rad) and transferred to a PVDF membrane using a semi-dry blotting apparatus ...
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bioRxiv - Molecular Biology 2022Quote: ... the pellets were re-dissolved in 1 M NaOH and quantified by Bradford assay (Quick StartTM Bradford Kit, BIO-RAD), and the supernatants containing cADPR were neutralized with Chloroform/Tri-n-octylamine mixture (volume ratio 3:1 ...
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bioRxiv - Plant Biology 2022Quote: ... 1 µg of total RNA were treated with iScript™ gDNA Clear cDNA Synthesis Kit (Bio-Rad; https://www.bio-rad.com/) for genomic DNA digestion and first-strand cDNA synthesis ...
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bioRxiv - Cancer Biology 2022Quote: ... A total of 1 µg RNA was used for cDNA synthesis with the IScript cDNA Synthesis Kit (Bio-Rad, 1708890), according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were thawed on ice and diluted 1:1000 in 1X Bradford reagent (BioRad, Hercules, CA, Cat. No. 500-0006). Standard curve was determined via serial dilutions of 1 mg/mL BSA diluted in H2O ...
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bioRxiv - Physiology 2024Quote: ... Total RNA (1 μg) was reverse transcribed to cDNA using an iScript cDNA synthesis kit (Cat no. 170884, BioRad, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... The mixture was then transferred to a 1 mm cuvette (ice chilled) for electroporation at 1.5 kV for 5 ms using an electroporator (Bio-Rad). Electroporated samples were mixed with 1 ml of SOC medium and transferred into a culture tube containing 1 ml of Brucella broth (BD) ...
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bioRxiv - Neuroscience 2024Quote: ... dissolved in 1X Tris-buffered saline (TBS) for 1 hour at room temperature prior to the addition of 0.05% Tween 20 (Bio-Rad) and primary antibodies overnight at 4°C ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were then washed with TBST and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Neuroscience 2024Quote: ... 72°C (30s) and 1 cycle of 72°C for 5 min (C1000 Touch Thermal Cycler, Bio-Rad, Hercules, CA). 2–5 μl of the PCR reactions were subjected to gel electrophoresis using a 3% agarose gel in tris-borate-ethylenediamine tetraacetic acid buffer ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were spun down at maximum speed for 1 minute and run on precast 4-20% gradient acrylamide gels (BioRad) in 1X Tris-Glycine buffer containing 0.1% SDS ...
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bioRxiv - Plant Biology 2024Quote: ... prior to bombardment with 10 mg 1 μm tungsten beads treated with 5 μg purified plasmid DNA in a Helios gene gun (BioRad) at 1,550 PSI ...
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bioRxiv - Microbiology 2024Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 hr at 4°C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 0.5 μM of gene-specific primers (Sup Table 1) in a CFX96 qPCR Real-Time PCR module with C1000 Touch Thermal Cycler (BioRad). After 10 minutes at 95°C ...
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bioRxiv - Developmental Biology 2024Quote: ... PVDF membranes were blocked for 1 hour in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4 °C with antibodies listed in Supplementary Table 2 in 5% BSA in TBS-T ...
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bioRxiv - Biophysics 2024Quote: ... The concentration of TP 70 was determined by measuring A230 (1 A230 unit = 240 pmol TP70) by SmartSpec Plus spectrophotometer (BIORAD). Aliquots were flash frozen and stored at −80°C.
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A synthetic elastic protein as molecular prosthetic candidate to strengthen vascular wall elasticitybioRxiv - Cell Biology 2023Quote: ... The membrane was rinsed three times with TBS-T and then incubated with goat anti-mouse IgG coupled to horse radish peroxidase (1/10,000, 1706516, Bio-Rad) in TBS-T for 1 h at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... HRP-conjugated secondary antibodies used were anti-rabbit and anti-mouse at a dilution of 1:2000 and immunoblots were developed using Chemidoc imaging system (ChemiDoc, BioRad).
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bioRxiv - Microbiology 2023Quote: ... or a median 750 ng (for HIV-1) of genomic DNA was combined with ddPCR Supermix for Probes (no dUTPs, BioRad), primers (final concentration 900 nM ...
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bioRxiv - Immunology 2023Quote: The previously obtained plasma (1 µl) and gut mucus (30 µl) were separated on a 4-15% Mini-PROTEAN TGX gel (BioRad) under non-reducing conditions ...
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bioRxiv - Molecular Biology 2023Quote: ... PVDF membranes were blocked for 1 hr in 5% non-fat milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T, Bio-Rad), then incubated overnight at 4°C with antibodies listed in Table S1 in 5% BSA in TBS-T ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Proteins of the parasite pellets were extracted using lysis buffer (1 mM DTT, 2X Laemmli buffer (Bio-rad, 161-0737) and protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2023Quote: ... in 1× TGX buffer and then transferred to nitrocellulose membrane using a Trans-Blot® Turbo™ Transfer System (BioRad). Subsequently ...
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bioRxiv - Molecular Biology 2022Quote: ... Amplification was performed in the presence of 1 mM of each oligonucleotide and iTaq Universal SYBR Green Supermix (Bio-Rad). Relative amounts of targets were calculated using the ddCT method ...
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bioRxiv - Immunology 2023Quote: ... Resultant cDNA at 1:100 dilution was subject to qPCR performed using iQ SYBR Green Supermix (BioRad, Cat. No. 1708880) on a QuantStudio 3 Real-Time PCR System (Applied Biosystems ...