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Citations for Bio-Rad :
3551 - 3600 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: cDNA synthesized from cells and animal tumors tissues was used for real-time PCR performed on iCycler (Bio-Rad) using a 2x SYBR Green master mixture (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... The isolated placental CD34+ single cell suspension was incubated with human CD34- phycoerythrin (PE) (Bio-Rad; dilution 1:25), human CD45-FITC (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... 5 μg of in vitro-transcribed RNA (2.5 μg of SNX27/LRFN2 RNA and 2.5 μg of the defective helper plasmid) was electroporated into 0.6×107 BHK-21 cells using a Gene Pulser II electroporation system (BioRad) in a gene pulser cuvette (0.2 cm gap) ...
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bioRxiv - Biochemistry 2020Quote: ... Onion epidermal cells were bombarded with 5 μg of each DNA construct using a helium biolistic gun (BIO-RAD). Treated epidermal cells were kept in the dark at room temperature for 16 h before analysis by confocal microscopy (Confocal Olympus FV 1000) ...
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bioRxiv - Microbiology 2020Quote: ... Protein concentrations in cell-free extracts and purified protein preparations were quantified using Bradford reagent (catalog # 5000006, Bio-Rad), with ovalbumin as a standard ...
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bioRxiv - Microbiology 2020Quote: ... and the images were analyzed and the signals quantitated after total cell protein normalization using the Image Lab 6.0.1 software (Bio-Rad).
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bioRxiv - Neuroscience 2022Quote: Cell and brain lysates were resolved using SDS polyacrylamide gel electrophoresis (PAGE) on 4-20% Mini-Protean Gel (BioRad), followed by immunoblotting for NMNAT2 (1:100 anti-NMNAT2 (B-10 ...
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bioRxiv - Genetics 2022Quote: ... The separated protein samples were transferred to the PVDF membranes using a Mini Trans-Blot Electrophoretic Transfer Cell (Biorad) at 100V for 1h ...
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bioRxiv - Immunology 2022Quote: ... Protein concentration in cell lysates was determined by using a standard Coomassie Bradford protein assay (Bio-Rad, Hercules, CA). For SDS-PAGE cell lysates were mixed with an equal volume of 2X SDS sample buffer (50 mMTris ...
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bioRxiv - Immunology 2022Quote: ... culture supernatants were collected and cells were directly lysed with 150 μl of 2× laemmli buffer (Bio-rad, 1610737). Proteins in culture supernatants and cell lysates were separated by SDS-PAGE and transferred onto PVDF membranes by using the Trans-Blot Turbo system (Bio-rad) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Then each pLC10 was introduced in the cells by electroporation using 0.1 cm cuvettes and 1.8 kV pulse (MicroPulser Electroporator, Biorad Spain). Transformants were selected on LB agar plates with kanamycin 250- 512mg/L or apramycin 30mg/L for each case ...
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bioRxiv - Microbiology 2019Quote: ... the infected cell lysates were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl ...
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bioRxiv - Microbiology 2019Quote: ... Protein lysates were separated in 10% SDS-PAGE gel (Transgen, Beijing, China) by Mini-PROTEAN tetra Cell system (BioRad) and transferred to PVDF membrane ...
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bioRxiv - Cancer Biology 2019Quote: ... each well was also photographed (bright-field) using a ZOE™ Fluorescent Cell Imager (BIO-RAD, Hercules, CA, USA).
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bioRxiv - Immunology 2019Quote: ... Cells were then stained for inflammasome activation using a caspase-1 FAM-YVAD-FMK FLICA probe kit (Bio-Rad). Cells were then processed for analysis by flow cytometry.
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bioRxiv - Genetics 2020Quote: ... Whole cell lysate protein samples (20-50 µg) were resolved on Criterion 4-20% gradient denaturing gels (Bio-Rad), transferred to nitrocellulose membranes (Bio-Rad ...
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Adipocyte vesicles: ‘all-in-one’ packages that stimulate tumor mitochondrial metabolism and dynamicsbioRxiv - Cancer Biology 2019Quote: ... Cells were lysed and protein concentration was determined using the DC™ Protein Assay from Biorad (Hercules, Ca, USA) for data normalization.
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bioRxiv - Biochemistry 2019Quote: ... Proteins in whole-cell lysates were separated on 4-15 % Mini-Protean TGX Precast gels (456-1085, Bio-Rad) and transferred to 0.2 μm Nitrocellulose membranes (1704158 ...
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bioRxiv - Molecular Biology 2020Quote: ... Equal mass of each cell extract (20 μg) was separated on a 4-20% Mini-PROTEAN TGX gel (BioRad) and then transferred to Immobilon P membrane (Millipore ...
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bioRxiv - Molecular Biology 2020Quote: ... The supernatant was then centrifuged through a 0.45-micron filter at 4,000 x g and the resulting cell extract was measured using the DC-Lowry assay (BioRad). One milligram of total cellular protein was then incubated with 30 μl bed volume of anti-V5 beads (Sigma) ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were then separated on 12 % polyacrylamide gels (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France) and transferred to 0.2 μm nitrocellulose blotting membrane (GE Healthcare ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were transferred to methanol-treated PVDF membranes using a Mini Trans-Blot Cell wet-transfer apparatus (BIO-RAD), according to manufacturer’s specifications at 400 mA for 1.5 hours ...
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bioRxiv - Microbiology 2020Quote: ... 15 % methanol (v/v) in distilled water) for 1 hour at 100 volts (Mini Trans-Blot Cell, Bio-Rad). The nitrocellulose membrane was blocked in blocking buffer (5% (w/v ...
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bioRxiv - Genomics 2021Quote: ... To confirm that the cells were lysed we took an aliquot and stained them with Trypan Blue (Bio-Rad) to visualize under the microscope ...
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bioRxiv - Cancer Biology 2021Quote: ... Whole cell lysates were separated through 7.5% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (Bio-Rad Laboratories, Inc.). Membranes were blocked with 5% milk powder in 0.1% Tween 20 in 1x Tris-Buffered Saline (TBST ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells resuspended in PBS+/+ were transfected with 100-150 μg DNA constructs (Gene Pulser Xcell System; Bio-Rad; 220V ...
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bioRxiv - Genomics 2020Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Immunology 2020Quote: ... Mono-Mac-6 effector cells previously stimulated with recombinant human IFN-ɣ (Bio-Rad, Hercules, CA, USA; cat. PHP050) at 0.2 µg ml-1 for 3h at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... whole pieces of mouse jejunum were homogenized with a bead beater in Bio-Plex Cell Lysis buffer (Bio-Rad). Protein concentrations were determined using the DC Protein Assay (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... Cells and plasmid DNA in solution were transferred into 0.2 cm generic electroporation cuvettes (Biorad Gene Pulser; Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: Total protein concentration of each cell lysate sample was determined by Bradford assay with Protein Assay Dye (Bio-Rad) on a UV/Visible spectrophotometer (Ultrospec 2100 pro ...
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bioRxiv - Neuroscience 2022Quote: ... GFP+ cells were captured into single tube with 100 μl lysis buffer containing Proteinase K and DNase (Bio-Rad). The sorted cells were then incubated for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... the gel was transferred onto a nitrocellulose membrane using Trans Blot Sd Semi-Dry Transfer Cell (BioRad Cat#1703940) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... The protein concentrations of culture medium and cell lysate were determined by the Bradford assay (Bio-Rad, Hercules, CA). Culture medium or cell lysate samples were resuspended in SDS sample buffer containing β-mercaptoethanol and incubated at 95°C for 5 min ...
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bioRxiv - Microbiology 2022Quote: ... Virus was then inactivated and whole cell lysates taken by the addition of 2× Laemmli buffer (Bio-Rad #161073) + 2-mercaptoethanol (Bio-Rad #1610710 ...
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bioRxiv - Plant Biology 2022Quote: ... Protein extracts were separated on homemade 4-20% gradient SDS polyacrylamide gels and blotted to a nitrocellulose membrane (Amersham Protran 0.2 μm NC) using a Trans-Blot SD Semi-Dry Transfer Cell (BioRad). Blotting efficiency was assessed by staining total protein with Ponceau S ...
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bioRxiv - Physiology 2022Quote: ... proteins were separated by running the gel in an electrophoresis chamber (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad) at 200 V for 30 min ...
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bioRxiv - Molecular Biology 2022Quote: ... and then imaged under the green channel of a ZOE Fluorescent Cell Imager (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Neuroscience 2022Quote: ... Gel electrophoresis and membrane transfer for Western blotting was accomplished using the Mini-PROTEAN Tetra Cell system (BioRad, 1660828EDU) with 2x Laemmli sample buffer and 2-Mercaptoethanol (BME [BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... and 0.0002% bromophenol blue at 95°C for 5 minutes and separated in pre-casted 12% or 7.5% Tris-Glycine polyacrylamide gel using the Criterion cell system (Biorad). Electrophoresis was conducted at 80 V in a Tris-Glycine buffer (25 mM Tris ...
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bioRxiv - Microbiology 2023Quote: ... Competent cells were subjected to electroporation using a pulse of 2.5 kV (MicroPulser, Bio-Rad Laboratories, Hercules, CA, USA), followed by recovery in 2 mL of ½ YTSS medium for 12 hours at 30 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... Electroporation was performed in 0.1 cm (The Cell Projects) electroporation cuvettes using a Gene Pulser II Electroporator (Bio-Rad) using the following parameters – 12.5 kV/cm ...
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bioRxiv - Cancer Biology 2023Quote: Plasmid DNA and siRNA oligonucleotides were transfected into U373 cells using electroporation with a GenePulser Xcell (Bio-Rad Laboratories). Electroporation was performed according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... cell media was replaced with 200 uL growth assay media (180 uL DME + 20 uL Alamar Blue (BioRad BUF012A), and incubation was continued for 4 hr at 37°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins in cell lysates were heated at 95[ for 5 minutes in Laemmli samples buffer (Bio-Rad, Hercules, CA), resolved by 10% SDS-PAGE ...
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bioRxiv - Bioengineering 2024Quote: ... Transformation of yeast cells by electroporation was carried out as in [27] with a Gene Pulser electroporator (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... The resolved proteins were transferred onto nitrocellulose membranes (Amersham) through a Trans-Blot semi-dry transfer cell (Bio-Rad). Membranes were subsequently blocked in Tris-buffered saline plus 0.1% Tween (TBS-T ...
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bioRxiv - Biochemistry 2023Quote: ... and cells were lysed in 350 μl/well lysis buffer from the AurumTM total RNA Mini Kit (Biorad, 7326820). The lysates were immediately frozen at −80 °C until RNA extraction ...
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bioRxiv - Microbiology 2023Quote: ... SDS PAGE was run in a Mini-Protean Tetra Vertical Electrophoresis Cell (Bio-Rad Laboratories, Incs., Hercules, CA, USA) using a 17% polyacrylamide gel and the PageRuler prestained protein ladder (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... 25 µl cell lysate and 50 µg protein for tissue lysates was loaded on 4-15% (Bio-Rad, 5678084) and 10% TGX Stain-Free Protein Gels (Bio-Rad ...