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Citations for Bio-Rad :
3351 - 3400 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Cytoplasmic extract prepared from the harvested cells was used for immunoprecipitation (IP) using ProteinG magnetic beads (Biorad). Beads were first equilibrated with IP buffer (25 mM Tris-Cl ...
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bioRxiv - Biophysics 2023Quote: ... Concentrated conditioned media normalized to cell numbers were loaded on 4-15 % SDS-PAGE gels (Bio-Rad) and ran at a voltage of 180V under room temperature for electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... 10 µl of normalized cell culture was spotted on 0.2 µm pore-size nitrocellulose membrane (Bio-Rad) and allowed to dry ...
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bioRxiv - Cell Biology 2023Quote: ... 800 μL of ‘rolled cells’ were gently transferred to a 4 mm-gap electroporation cuvette (BioRad; #1652088). Electroporation was then performed using the following settings ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed twice and resuspended in wash buffer for data acquisition at Ze5 cytometer (BioRad).
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bioRxiv - Cancer Biology 2022Quote: ... for 5 minutes followed by visualization under the ZOE™ Fluorescent Cell Imager at 590nm (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... ∼3 million cells were harvested and proteins were extracted by boiling in 2x Laemmli sample buffer (BioRad). Protein levels were measured by Western blotting with different antibodies (Rabbit anti- BRWD3 ...
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bioRxiv - Systems Biology 2023Quote: ... then cells stained for analysis by flow cytometry 9 or 10 days after dCas9 infection (BioRad ZE5). In some cases ...
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bioRxiv - Systems Biology 2023Quote: ... Time points were measured every 2-3 days by flow cytometry analysis of >10,000 cells (BioRad ZE5). For experiment with BRM014 (MedChemExpress #HY-119374) ...
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bioRxiv - Genetics 2023Quote: ... and then transferred onto a 0.45-µm nitrocellulose membrane in a Mini Trans-Blot Cell (Bio-Rad) containing 25 mM Tris ...
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bioRxiv - Cancer Biology 2023Quote: Cells were lysed in sample buffer (2× Laemmli sample buffer, No. 1610737, Bio-Rad, Hercules, California, USA). Whole-cell lysates were subjected to sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... Human AAVS constructs were transfected into CHO cells using the Gene Pulser MXcell Electroporation System (Bio-Rad). Transfected cells were selected for with 10µg/mL puromycin or 1mg/mL neomycin ...
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bioRxiv - Physiology 2023Quote: ... each fiber was carefully submerged in 3 µL of lysis buffer (SingleShot Cell Lysis kit, Bio-rad), containing proteinase K and DNase enzymes to remove unwanted proteins and DNA ...
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bioRxiv - Plant Biology 2023Quote: ... samples were subjected to a Bio-Rad Mini-Protean III slab cell (Bio-Rad, Richmond, CA, USA), with a discontinuous buffer system (Laemmli et.al 1970 ...
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bioRxiv - Microbiology 2024Quote: ... The protein concentration in the cell lysates was determined using a DCTM protein assay kit (BioRad, 5000111). The same amounts of proteins were run on 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (BioRad ...
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bioRxiv - Molecular Biology 2023Quote: ... Cell debris was pelleted by microcentrifugation at 4°C and protein levels quantified using Bradford Assays (BioRad) as previously described (38) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with cold PBS and crosslinked in a GS Gene Linker (Bio-Rad Laboratories) using 0.8 J/cm2 of 254 nm UV light ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell protein content was determined with the Bio-Rad protein quantification reagent (Bio-Rad Laboratories, Inc.). Caspase-3 activity was assessed in duplicates via a fluorogenic assay using the Ac-DEVD-AMC-specific caspase-3 substrate (Calbiochem ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in the cell lysate was quantified using the Bradford protein assay (#500-0006, Bio-Rad). 30 μg of each sample was boiled with 4x Laemmli Sample Buffer containing 10% 2-mercaptoethanol (#1610747 ...
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bioRxiv - Molecular Biology 2023Quote: ... 20% v/v methanol)) using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, CA, USA) apparatus {20V ...
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bioRxiv - Bioengineering 2024Quote: ... of dimensions (8.6 X 6.7 cm) using a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) at 100V with a constant current for 60 mins at 4°C ...
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bioRxiv - Developmental Biology 2023Quote: ... Proteins were transferred to a PVDF membrane using a Mini Trans-Blot® Cell (Bio-Rad Laboratories) at 100V for 2h ...
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bioRxiv - Microbiology 2023Quote: ... 7 µg of each cell extract was loaded on a 12% stain-free SDS-PAGE gel (BioRad) and separated by gel electrophoresis at 175 V for 45 min ...
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bioRxiv - Evolutionary Biology 2024Quote: ... we analyzed the fluorescence intensity of the nuclei using an S3e Cell Sorter (BIO-RAD, Hercules, CA). The cell sorter excited the PI with a 488 nm laser and detected its emission with a 615/25 nm band pass filter ...
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bioRxiv - Cancer Biology 2024Quote: ... the cells were imaged using a fluorescence microscopy (ZOE™ Fluorescence Imaging, Bio-Rad, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2024Quote: Protein estimation was performed in cell lysates by BCA protein assay kit (Bio-Rad, Hercules, CA, USA). For Western blots ...
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bioRxiv - Biochemistry 2024Quote: ... at 20V for 1h in a semi-dry Trans-Blot SD cell system (Bio-Rad, Hercules, CA). Following the blockade of the membranes using TBS-T (20 mM Tris ...
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bioRxiv - Microbiology 2020Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentration of cell lysates was determined by using the 595 nm Protein Assay Dye Reagent (Bio-Rad). Western blots were carried out after 9% SDS-PAGE ...
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bioRxiv - Cell Biology 2019Quote: ... Cell lysates were cleared with centrifugation and protein concentrations were measured with Protein Assay kit (Bio-Rad, #5000001). 25-50 μg protein were loaded to 10-well SDS-PAGE gradient gel (Bio-Rad 456-1094) ...
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bioRxiv - Cell Biology 2020Quote: ... Whole-cell lysates were separated on SDS-PAGE on 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2020Quote: ... Protein concentrations in the cell lysates were estimated using the Quick Start™ Bradford Dye Reagent (Biorad# 5000205) followed by blocking of free thiol groups in HEN buffer containing 0.2% MMTS and 2.5% SDS at 50°C for 30 minutes ...
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bioRxiv - Microbiology 2020Quote: ... Whole cell lysates were resolved by SDS-PAGE in 4-20% gradient Mini-PROTEAN TGX gels (Bio-Rad) and transferred to 0.2 μm pore size nitrocellulose membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2019Quote: ... Total cell lysates were separated on 8-12% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). Membranes were blocked with TBST with 5% (w/v ...
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bioRxiv - Biophysics 2020Quote: HEK293T cells cultured in 12 cm dishes were transfected with 5 μg plasmid using Transfectin lipid reagent (Biorad) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... was carried out for 90 min at 25 W in a Mini Trans-Blot Electrophoretic Transfer Cell (BioRad). Membranes were blocked for 3 h with 5% w/v nonfat dry milk in 0.05% Tween 20 in PBS (PBS-T ...
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bioRxiv - Molecular Biology 2020Quote: ... Whole cell lysates were loaded into 4-20% Criterion TGX Precast 18 well gels (Bio-Rad, cat # 5671094) and separated by electrophoreses at 120 V for 1 hr ...
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bioRxiv - Molecular Biology 2020Quote: ... 0.1% SDS) and transferred onto nitrocellulose membranes (Protran) with a Trans-Blot SD semidry transfer cell (Bio-Rad). Total proteins were revealed by Pierce® Reversible Stain (Thermo Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... After 2 weeks cells were stained by the sulforhodamine B and photographed by E-Gel Imager (Bio-Rad). The cloning numbers in each group were counted to calculate the inhibition rate.
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bioRxiv - Molecular Biology 2021Quote: ... Gel electrophoresis was performed at 4°C using Wide Mini-Sub Cell GT Horizontal Electrophoresis System (BioRad, 1640301) at 14 V (approximately 300 mA ...
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bioRxiv - Bioengineering 2019Quote: ... PAGE separation was carried out in 1 mm vertical mini gel format (Bio-Rad Mini-PROTEAN Tetra Cell) with a discontinuous buffer system under denaturing conditions ...
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bioRxiv - Developmental Biology 2019Quote: ... the single cell suspension prepared above were immediately counted using a hemocytometer (TC20, Bio-Rad, Hercules, CA, USA) and the cell concentrations were adjusted to 1000 cells/μl prior to barcoding ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were harvested and proteins resolved by 10% SDS-PAGE and transferred to PVDF membrane (BioRad, Hercules, CA). Non-specific binding sites were blocked with 5% (w/v ...
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bioRxiv - Microbiology 2019Quote: RNA was extracted from infected cells at various time intervals after infection using Total RNA mini kit (Biorad). For RT-qPCR ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of whole cell extracts and tissue lysates were determined using a Bradford Protein assay (Bio-Rad). 50μg of sample proteins per well were subjected to SDS-PAGE and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cell debris were spun down and the supernatant loaded onto a 1D SDS min-gel (BioRad Laboratories, US). We visualized the gel by Coomassie staining and excised the region corresponding to the molecular weight of Tdrd7 protein for subsequent MS-Western analysis.
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bioRxiv - Cancer Biology 2021Quote: 2 ⨯ 106 CAR T cells collected after 24-day expansion were resuspended with 1 ⨯ Laemmli sample buffer (BIORAD) containing 2.5% β-mercaptoethanol before sonication with 50% amplitude in Sonic Dismembrator (FisherBrand) ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by electrophoresis at 17 V for 45 min at 4 °C (Mini-Sub® Cell GT, BIORAD). Slides were transferred to DNA precipitation solution for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2021Quote: ... Cell lysates and virion lysates were resolved on 8 to 16% Mini-Protean TGX precast gels (Bio-Rad), transferred onto nitrocellulose membranes (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... or 12% SDS polyacrylamide gel electrophoresis (SDS-PAGE) using Mini- PROTEAN® Tetra Cell (Bio-Rad, CA, USA). After migration ...