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Citations for Bio-Rad :
3651 - 3700 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... Donor CD103+ KLRG1-CD8 T cells from LP of transplanted tissue were identified following the gating strategy in FigureS11 and single cells were sorted into 96-well plates (BioRad) containing 5 µL capture buffer (20 mM Tris-HCl pH8 ...
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Inhibition of nucleotide synthesis promotes replicative senescence of human mammary epithelial cellsbioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4– 15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h ...
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bioRxiv - Molecular Biology 2020Quote: DNA isolated from cells by phenol:chloroform isolation and ethanol precipitation was cleaned on Micro Bio-Spin P-6 SSC columns (Bio-Rad) according to manufacturer protocol ...
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bioRxiv - Cell Biology 2019Quote: ... and 25-30 μg of protein were loaded on a 7.5% separating gel using Mini Trans-Blot Cell (Bio-Rad). Nitrocellulose membranes were stained with primary antibodies ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h and then incubated with primary overnight and secondary antibodies for 2 h ...
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bioRxiv - Biochemistry 2019Quote: Trypanosome whole cell protein lysates (2×106 cells per well) were separated using SDS-PAGE (10% gels) and transferred by semi-dry blotting (BioRad) for 45 min at 25 V to PVDF membranes ...
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bioRxiv - Microbiology 2019Quote: Approximately 1 × 105 bacterial cells were suspended in PBS containing 1% (w/v) PBS-BSA and incubated with fH (Biorad) at a final concentration of 5 μg/mL for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2019Quote: ... Whole-cell lysates were resolved by SDS–PAGE on 4–15% gradient gels and blotted onto nitrocellulose membranes (Bio-Rad). Membranes were blocked for 1 h in nonfat-milk ...
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bioRxiv - Microbiology 2019Quote: ... Approximately 2-10 μl of unmethylated DNA (>50 ng/μl) was added to cells and transferred to a 0.4 cm gap electroporation cuvette (Bio-Rad). Cells were exposed to 2.5kV for 4-6 msec ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were resuspended in 1X PBS and mechanically lysed by sonication before protein standardized by DC assay (BIO-RAD) and mixed with Western blot sample loading buffer (2X loading buffer (LI-COR) ...
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bioRxiv - Plant Biology 2020Quote: Transient expression in onion cells (bulb sliced to ~16 cm2) was conducted using the Biolistic PDS 1000/He Particle Delivery System (Biorad) (1100 psi ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were then transferred onto a PVDF membrane using a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad) per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The co-IP samples and input (1% of whole cell lysates) were boiled in 2× Laemmli sample buffer (Bio-Rad) supplemented with 2-mercaptoethanol (Bio-Rad ...
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bioRxiv - Bioengineering 2020Quote: ... DGF-298 electrocompetent cells were transformed with 300 ng of the purified PCR product (described above) by electroporation in a Genepulser XCell (Biorad) at 1800 V ...
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bioRxiv - Bioengineering 2020Quote: ... Samples were separated by SDS-PAGE using a Mini-Protein Tera Cell system (Bio-Rad Laboratories, Inc., Hercules, USA, CA). Proteins were visualized by CBB staining as described elsewhere.
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bioRxiv - Biochemistry 2020Quote: ... by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany). Proteins were transferred to a nitrocellulose membrane (Millipore ...
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... The quantification of protein content in exosome suspensions and whole-cell lysates was undertaken by a modified Lowry assay (DC Protein Assay, BIORAD). For immunoblotting ...
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bioRxiv - Cancer Biology 2021Quote: ... 48 cytokines were quantified in the cell supernatant using a Bio-Plex Pro Human Cytokine Screening Panel (Bio-Rad, #12007283) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... based on the manufacturer’s protocol and 30 μg of protein lysate of each sample was separated by electrophoresis in 7.5% or 12% SDS-PAGE gel and transferred into 0.45 μm PVDF transfer membrane using a Semi-Dry transfer cell (Trans-Blot SD, Bio-Rad). After transfer ...
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bioRxiv - Cancer Biology 2019Quote: RNA was isolated from cell lines or flash-frozen tissue as described above was reverse-transcribed using iScript (Bio-Rad). Real-Time PCR was conducted using VeriQuest SYBR Green qPCR Master Mix (Affymetrix) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were separated by 10% SDS-PAGE followed by electrophoretic transfer to polyvinylidene difluoride membranes (Bio-Rad, Hercules CA). Membranes were blocked with 5% skim milk in PBS containing 0.1% Tween 20 and incubated at 4°C overnight with the TRPM4 antibody ...
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bioRxiv - Cell Biology 2021Quote: ... Gels were Western blotted overnight with constant current of 200 mA using a Trans-Blot® Cell (Bio-Rad, USA). Membranes were destained from residual Coomassie traces by washes in methanol for several minutes ...
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bioRxiv - Microbiology 2020Quote: ... The slides were read under a fluorescence microscope and photographs were taken with a ZOE™ fluorescent cell imager (BioRad).
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bioRxiv - Microbiology 2021Quote: ... Lysates were run on 12% polyacrylamide gel and transferred to a nitrocellulose membrane using the Trans-blot SD semi-dry transfer cell (Biorad). To represent the same number of cells in each lane ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were in-gel rehydrated for 16 hrs and isoelectrically focused on 7 cm pH 3-10 IPG strips to 10,000 Vh on a Protean® IEF Cell (BioRad). After focusing ...
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bioRxiv - Microbiology 2019Quote: ... proteins were transferred to a polyvinylidene fluoride (PVDF) membrane using the Mini Trans-Blot Electrophoretic Transfer Cell system (Bio-Rad) per the manufacturer’s instructions and blocked with 100 mg/mL skim milk in 1x TBS/Tween 20 ...
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bioRxiv - Cancer Biology 2020Quote: HEL 92.1.7 cells were transfected by electroporation at various conditions using the Gene Pulser Xcell™ Electroporation System (BioRad, USA). In brief ...
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bioRxiv - Cancer Biology 2021Quote: ... RNP complexes were added to 200,000 cells in 200µL electroporation buffer (0.2 cm cuvette) and electroporated via the Gene Pulser Xcell™ Electroporation System (Bio-Rad) using exponential decay pulses (145V ...
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bioRxiv - Cell Biology 2021Quote: ... separated by SDS-PAGE (12% T, 1 mm, 200 V; BioRad mini protean III cell; BioRad Laboratories GmbH, München, Germany) and stained with Coomassie Brilliant Blue G-250.
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bioRxiv - Cell Biology 2020Quote: ... Each DNA construct was validated by sequencing and transfected into cells using an electroporator (GenePulser xCell, Bio-Rad— Hercules, CA). Clonal cell lines were created by selection and limiting dilution ...
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bioRxiv - Biochemistry 2022Quote: ... by loading samples onto a 0.5X TBE 5% polyacrylamide gel prepared for a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad) using ProtoGel acrylamide (National Diagnostics) ...
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bioRxiv - Immunology 2022Quote: Live CD3+CD8-CD4+ cells were sorted (for gating strategy see supplemental figure 1A) into 384-well hard shell plates (Biorad) with 5 μl of vapor-lock (QIAGEN ...
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bioRxiv - Immunology 2022Quote: Live CD3+CD4+CD25+CD127low cells were sorted from fresh SF (Supplementary figure 1a) into 384-well hard shell plates (Biorad) with 5 μl of vapor-lock (QIAGEN ...
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Construction of a constitutively active type III secretion system for heterologous protein secretionbioRxiv - Synthetic Biology 2022Quote: ... samples were separated by size on 12.5% SDS-PAGE gels run at 150V for 60 minutes using the Mini-PROTEAN Tetra Cell system (Bio-Rad). The proteins were transferred to a polyvinylidene fluoride membrane (PVDF ...
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bioRxiv - Systems Biology 2022Quote: ... Protein extracts derived from OBC1 cells were electrophoretically transferred onto nitrocellulose membranes using a Trans-blot Turbo transfer system (up to 25V, 7min) (BioRad). Membranes were probed with primary antibodies at 1:1000 dilution in 5% nonfat milk or BSA according to manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... and cDNA (1.0 μL) of cell or EV were prepared in 20 μL reaction and run on CFX96 qPCR machine (Bio-Rad). Gene expression level were first normalized to housekeeping gene GAPDH and then calculated with ΔΔCt method ...
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bioRxiv - Microbiology 2022Quote: ... The proteins were transferred to polyvinylidene difluoride membrane (Milipore, Billerica, MA, USA) using a semi-dry chamber (Trans blot SD semi-dry transfer cell, Biorad) and blocked with 4% semi-skimmed milk in PBS (blocking buffer ...
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Proteolytic cleavage of the extracellular domain affects signaling of parathyroid hormone receptor 1bioRxiv - Pharmacology and Toxicology 2022Quote: ... Lysates were cleared by centrifugation and run on 10% SDS-polyacrylamide gels in a Mini-PROTEAN 3 cell apparatus (Biorad). Proteins were electroblotted onto Immobilon P membranes (Millipore ...
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bioRxiv - Cancer Biology 2022Quote: ... snap frozen tumoroid cell pellets were lysed in Phosphatase-substituted RIPA buffer and run on a 10 % precast gel for P53 detection (BioRad). Protein levels of P53 (1:1.000 ...
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bioRxiv - Cell Biology 2022Quote: ... The whole cell lysate and crude cytoskeletons were resolved on 4-20% Mini-PROTEAN tris-glycine polyacrylamide gels (Bio-Rad), and Western blots were stained with 1:5000 diluted anti-beta-actin mouse monoclonal antibody (Cell Signaling Technology ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast concentration was adjusted to 4 × 108 cells/ml and added to the same amount of 1.2% low melting agarose gel (Bio-Rad) solution ...
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bioRxiv - Microbiology 2020Quote: ... Protoplast suspensions (2 × 108 cells/ml) containing 0.6% low melting agarose gel were added to 50-well dispensable mold plates (Bio-Rad). Plugs were immersed in 10 ml of NDS (1% N-lauroyl sarcosinate sodium salt solution ...
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bioRxiv - Cell Biology 2021Quote: ... cells were lysed and normalised for protein concentration using the Bio-Rad Protein Assay Kit (Bio-Rad Laboratories, Inc. # 5000001) and a spectrophotometer ...
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bioRxiv - Neuroscience 2021Quote: ... We ran 40 µg of protein on 10% gels using the Mini-PROTEAN Tetra Cell western blotting system (Bio-Rad). Anti-NaV1.1 (Ab5204a ...
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bioRxiv - Biophysics 2021Quote: ... 100 ng of pTrcHis plasmid (with or without transcribed complementary target sequences) were added to 50 μl of competent cells in a 0.2 cm-gap Gene Pulser® electroporation cuvette (BioRad) on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... proteins were transferred to 0.45 μM nitrocellulose membranes using Trans-Blot® Turbo™ Transfer System Cell system (Bio-Rad). The membrane was then blocked in 5% nonfat dry milk TBS 0.05% Tween 20 and incubated with primary antibody overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Cell lysates were prepared using an iScript qRT-PCR sample preparation reagent (50 μL/well) (Bio-Rad Labs, California, CA) [31 ...
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bioRxiv - Molecular Biology 2022Quote: ... was performed the next day by using a constant amperage of 50 μA per strip in the Protean IEF Cell equipment (BioRad). Next ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins from cell culture were extracted by scraping the culture wells in the presence of RIPA buffer (BioRad, Hercules, CA) with protease inhibitor and phosphatase inhibitor (St ...