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Citations for Bio-Rad :
3601 - 3650 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2023Quote: ... and transferred onto a nitrocellulose membrane using a semi-dry blot (25V, 0,22A, 50 min, Trans-Blot SD Semi-Dry Transfer Cell, #1703940, Biorad) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... and transferred to nitrocellulose membrane by semi-dry blotting using the Trans-Blot Semi-Dry Transfer Cell (Bio-Rad) at 100 V for 2 h ...
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bioRxiv - Microbiology 2023Quote: ... Lysed cells were resolved on a 4% to 20% Mini-Protean TGX precast protein SDS-PAGE gel (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... 25 μL of cells were added to an electroporation cuvette (0.1 cm gap, Bio-Rad, catalog no. 165-2089), and electroporated using the Ec1 setting (1.8 kV ...
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bioRxiv - Neuroscience 2023Quote: Cortical and hippocampal brain tissue sections were lysed using the Bio-Plex cell lysis kit (Bio-Rad Laboratories #171304011) and protein concentrations were determined using a Pierce BCA Protein Assay (Thermo Fisher #23225) ...
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bioRxiv - Bioengineering 2022Quote: Total RNA was extracted and purified from RPE tissue punches using Single Shot Cell Lysis Kit (Bio-Rad Laboratories). cDNA synthesis was performed from 1.5 μg RNA using RT² First Strand Kits (Qiagen ...
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bioRxiv - Biochemistry 2022Quote: ... whole cell lysates were separated by SDS-PAGE and transferred to 0.45 um Immun-Blot PVDF membranes (BIO-RAD). Membranes were then blocked in 5% milk/TBST for 1 hour at room temperature and incubated with primary antibodies at 4 °C overnight ...
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bioRxiv - Immunology 2023Quote: ... RNA from the barcoded cells for each sample was reverse transcribed in a C1000 Touch Thermal cycler (Bio-Rad), and libraries generated according to the manufacturer’s protocol with no modifications (14 cycles used for cDNA amplification) ...
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bioRxiv - Microbiology 2023Quote: ... Infected cells were then washed three times with PBS and lysed in 1x Laemmli Sample Buffer (Bio-Rad #1610747) containing 100mM DTT ...
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bioRxiv - Cancer Biology 2023Quote: ... and equal amounts of cell extracts were separated by SDS 12% PAGE and transferred to nitrocellulose membrane (Bio-Rad). Samples were then analyzed by Western blotting using a rabbit-raised anti-p53 polyclonal antibody (CM1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell lysates (50 µg) were loaded into 4–20% PROTEAN® TGX™ Precast Protein Gels (BioRad, Cat# 5678081). After overnight transfer to nitrocellulose membranes ...
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bioRxiv - Microbiology 2023Quote: ... using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Plasmid sequences were confirmed by sanger sequencing.
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bioRxiv - Microbiology 2023Quote: ... Each 100 μl aliquot was transformed with 0.1 pmol of EZ-Tn5
Tnp Transposome using 2 mm electroporation cuvettes (Cell Projects, EP-102) and electroporation program Ec2 (V = 2.5 kV) with MicroPulser Electroporator (Biorad). Each reaction was allowed to recover in 500 μl SOC (2% tryptone ... -
bioRxiv - Microbiology 2023Quote: ... A total of 50 μL of cells was mixed with plasmids and then subject to electroporation (Bio-Rad, USA) at 2.5 kV ...
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bioRxiv - Synthetic Biology 2023Quote: ... After an incubation not exceeding five minutes the chilled DNA/cell suspension was filled into a pre-chilled 0.2-cm gap cuvette (BioRad) and electroporated using a Gene Pulse Controller electroporation system (Bio-Rad ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were subsequently transformed by electroporation with pTS055(ThrBF267D) (0.1 cm Gene Pulser Cuvette #165-2089 and Micropulser, BioRad). Cells were recovered in SOC medium with kanamycin and 1 µM aTc for Cas9 induction at 30°C for 2 h and streaked out on to LB agar plates with kanamycin ...
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bioRxiv - Cancer Biology 2023Quote: ... MDA-MB-231 cells were transfected with the CapG-GFP construct and its mutants using Transfectin (Biorad, Hercules, CA) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... pAc5.1-dTRPA1-A and pAc5.1-EGFP were transiently co-transfected into control or dAGPS stable cells using TransFectin Lipid Reagent (BioRad). After a 24 h incubation ...
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bioRxiv - Molecular Biology 2023Quote: ... through semi-dry transfer at 25 V for 30 min using Trans-BLOT SD Semi-dry Transfer Cell (BioRad). Tris-glycine buffer containing 20% methanol was used for transfer ...
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bioRxiv - Genomics 2023Quote: ... 10 μL of the mixture was then loaded onto a Dual-chamber Cell Counting Slide (Bio-Rad, Cat # 1450011), and the cells were counted using a Bio-RAD TC20 Automated Cell Counter ...
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bioRxiv - Microbiology 2023Quote: ... We added 1.0 mg of various CRISPRi plasmids to the 0.2 ml fusobacterial competent cells in a chilled cuvette (0.1 cm electrode gap, Bio-Rad), allowing it to rest on ice for 10 minutes ...
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bioRxiv - Molecular Biology 2024Quote: ... and equal amounts of cell extracts were separated by SDS 12% PAGE and transferred to nitrocellulose membrane (Bio-Rad). Samples were then analyzed by Western blotting using a rabbit-raised anti-p53 polyclonal antibody (CM1 ...
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bioRxiv - Immunology 2024Quote: ... the cells were adjusted to 7.5×105 cells/mL and stimulated with recombinant human interferon gamma (IFNγ, cat. no. PHP050, BioRad) at 0.2 μg/mL for 3 h at 37°C to activate the phagocytic ability of MonoMac-6.10 Meanwhile ...
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bioRxiv - Cell Biology 2024Quote: ... Cell lysates were analyzed with SDS–PAGE using 4-15% Mini-PROTEAN TGX Stain-Free Gels (4568083 Bio-Rad). Proteins were transferred to a nitrocellulose membrane (1704271 ...
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bioRxiv - Microbiology 2024Quote: Total bacterial DNA was extracted from LB broth cell pellets by InstaGene Matrix (Bio-Rad, Marnes-la-Coquette, France), and real-time ZKIR PCR was performed using Rotor-Gene Q (Qiagen ...
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bioRxiv - Molecular Biology 2024Quote: ... The proteins were transferred onto PVDF membranes using the Mini Trans-Blot Electrophoretic Transfer Cell (Bio-Rad, 170-3930) at 80 V ...
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bioRxiv - Molecular Biology 2024Quote: ... proteins were transferred onto a polyvinyldiene fluoride (PVDF) membrane in a Mini Trans-Blot cell (Bio-Rad, CA, USA) at a constant current of 315 mA on ice for 90 min ...
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bioRxiv - Genetics 2024Quote: ... Thirty micrograms of denatured cell lysate proteins were loaded for SDS-PAGE electrophoresis (4–20% TGX gel, Bio-Rad). Primary antibodies include ALDH3B2 (Proteintech ...
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bioRxiv - Genetics 2024Quote: ... were loaded and run on a mini gel system (Mini-PROTEAN Tetra Vertical Electrophoresis Cell, Bio-Rad REF-1658004) at 200V for 35-45 minutes ...
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bioRxiv - Immunology 2024Quote: ... pre-soaked in transfer buffer (20 mM Tris, 190 mM glycine, 20% Methanol, pH 8.3) using a Mini-Transfer cell (BioRad) for 1.5 hours (100 V constant ...
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bioRxiv - Bioengineering 2020Quote: ... A 10 μL cell-free sample was injected into a BioRad chromatographic column (Aminex HPX-87H, 7.8 mm 300 mm, Biorad, USA). The HPLC was equipped with a P680 pump ...
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bioRxiv - Biochemistry 2021Quote: ... Whole cell lysate samples containing 4 μg of protein were fractionated using a 4–20% SDS-PAGE gel (Bio-Rad). Proteins were transferred to a 0.2 μm nitrocellulose membrane using a Trans-Blot Turbo transfer system (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell viability was determined at indicated time points by adding 1/10 volume AlamarBlue solution (Biorad, Hercules, CA, United States) to each well ...
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bioRxiv - Systems Biology 2020Quote: ... 20 µg of each extract was analyzed by electrophoresis in 10% polyacrylamide gels and transferred to polyvinylidene difluoride membranes using Trans-Blot SD Semi-Dry Electrophoretic Transfer Cell (Biorad). After 1 h incubation at room temperature in Odyssey Blocking Buffer (TBS ...
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bioRxiv - Cancer Biology 2021Quote: ... whole cell lysates were separated on 8% sodium dodecyl sulfate (SDS) polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes (BioRad). The membranes were blocked and incubated overnight at 4 °C with a mouse monoclonal anti-FGFR1 antibody (1:100 ...
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bioRxiv - Cell Biology 2020Quote: ... The measurements were performed using the blue laser (488 nm) of the S3e Cell Sorter (Bio-Rad GmbH; Munich, Germany). The cell cycle phases were determined with the FlowJo (FlowJo ...
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bioRxiv - Immunology 2021Quote: ... samples were transferred to FACS polystyrene tubes using a disposable bacterial filter (50 μm, Sysmex) and macrophages were sorted into 100 μL of PBS using a S3TM Cell Sorter (BioRad). Isolated cells were verified by fluorescence microscopy and differential interference contrast.
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bioRxiv - Developmental Biology 2020Quote: ... and electrophoresed at 100-200 V for one to two hours in a Mini-PROTEAN Tetra Vertical Electrophoresis Cell (BIORAD). Proteins were transferred onto PVDF membranes (TransBlot Turbo Mini PVDF Transfer Packs ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... supplemented with 210 mM NaCl) to 200 µL of cell suspension and transferring into 0.4 cm gap electroporation cuvettes (Bio-Rad). The cells were electroporated at 350 µF/400 V using a Bio-Rad Gene Pulser II electroporation system ...
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bioRxiv - Microbiology 2022Quote: ... 1 µg of plasmid was added to electrocompetent GBS cells and transferred to a cold 1 mm cuvette (Fisher or BioRad). Electroporation was carried out at 2.5 kV on an Eppendorf eporator ...
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bioRxiv - Molecular Biology 2020Quote: ... cell viability was determined by measuring the absorbance at 490 nm using a 550 BioRad platereader (Bio-Rad, Hertfordshire, UK). At least triplicates were done for each condition to get statistical significance ...
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bioRxiv - Molecular Biology 2020Quote: ... ½ of a confluent T75 flask of WT F1 2-1 MS2129 female ESCs were electroporated with 40μg of PciI linearized PCR2.1-Puro XistΔE targeting plasmid (800v, 0.2ms, 4mm cuvette, Biorad X-Cell electroporation module) and plated at varying dilutions on 10cm plates of confluent irradiated DR4 feeders ...
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bioRxiv - Molecular Biology 2020Quote: ... were electroporated with 40μg of Not1 linearized paa2Δ1.7 plasmid DNA (800v, 0.2ms, 4mm cuvette using a Biorad X-Cell electroporation machine) and plated on confluent irradiated DR4 feeders ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were loaded at 100000 cells/lane and separated by electrophoresis under denaturing conditions using the Mini-Protean IITM system (BioRad) for 50 minutes at 180 V ...
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bioRxiv - Molecular Biology 2020Quote: Whole-cell lysates were run on 10 or 15% SDS-polyacrylamide gels and transferred to nitrocellulose membrane (1620097, Bio-Rad) by standard methods ...
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... cell were lysed with modified RIPA buffer and EP300 was co-immunoprecipitated using magnetic Sure-bead Protein G (Bio-Rad)-coupled p300 (clone 2033 ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA from the barcoded cells for each sample was subsequently reverse-transcribed in a C1000 Touch Thermal cycler (Bio-Rad) and all subsequent steps to generate single-cell libraries were performed according to the manufacturer’s protocol with no modifications ...
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bioRxiv - Plant Biology 2019Quote: ... The embedded cells were then lysed and DNA was separated using a CHEF Mapper® XA System (BioRad, Munich, Germany) essentially as previously described (Swiatek et al. ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Cancer Biology 2019Quote: ... total RNA was extracted from dissociated single cells using QIAGEN RNease Mini Kit and cDNA was prepared using 1µg RNA with iScript cDNA Synthesis Kit (Bio-Rad). SYBR green-based QPCR was performed using murine primers listed as below (GLI1 ...