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Citations for Bio-Rad :
3801 - 3850 of 4215 citations for 4F2 Cell Surface Antigen Light Chain SLC7A5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 μl of this cell suspension was then placed on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) TGY2X pad prepared on a cover glass using a gene frame (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... and 5 h later cells were stained with mAbs against CD4-PerCP-Cy5.5 (as in Table S2) and CD8β-FITC (clone PPT23, Bio-Rad). The cells were fixed and permeabilized with BD Cytofix/Cytoperm (BD Biosciences ...
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bioRxiv - Molecular Biology 2023Quote: Preparation of the electrocompetent DH5α cells and electroporation were done following the instruction manual of the micropulser electroporator (Bio-rad). After electroporation ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were then isolated on an iodixanol gradient and counted after addition of trypan blue using a TC20 automated cell counter (Biorad). 5.000-50.000 counted nuclei were transferred to a new tube and were centrifuged at 500g before proceeding with the transposition reaction ...
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bioRxiv - Cell Biology 2023Quote: ... Cell pellets were resuspended by addition of ice-cold PBS supplemented with 2% FBS and counted using a TC-20 cell counter (BioRad). 2.5-5x105 cells were harvested ...
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bioRxiv - Cell Biology 2023Quote: The protein concentrations of the cell lysates were determined using a Bio-Rad Protein Assay Dye Reagent (Bio-Rad, 5000006). Volumes of lysates containing the same amount of total protein were heated to 95°C for 5 minutes after addition of 1/4 volume of 4x SDS-PAGE Loading Solution (250 mM Tris ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF membrane (Carl Roth, T830.1) by using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad). The membrane was blocked with 5 % BSA (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of the cell-oligonucleotide mixture was transferred to a pre-chilled 1 mm electroporation cuvette (BioRad catalog #1652083) and transformed with 1.8 kV ...
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bioRxiv - Biochemistry 2024Quote: ... Cell lysates (30 μg) were separated on precast 4-15% or 7.5% SDS-polyacrylamide Criterion gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were resuspended in FACS buffer and acquired with a 5-laser Bio-Rad ZE5 flow cytometer (Bio-Rad). FACS data were analyzed using FlowJo version 10.9 software (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: T cells were washed once with ice-cold PBS and immediately lysed with 4x laemmli buffer (Bio-Rad, cat. 1610747). Cell lysates were separated by SDS-PAGE and blotted with anti-Kcnn4 antibody (cat ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Cell Biology 2024Quote: ... The cell lysates were fractionated by SDS-PAGE on a 10% gel or a 4-15% gradient gel (Bio-rad) and the separated proteins were transferred to a polyvinylidene difluoride membrane (Merck) ...
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bioRxiv - Cell Biology 2024Quote: ... and approximately 1 μg of plasmid DNA was electroporated into the cells by pulsing twice at 1000 V with the Xcell gene pulser (Biorad). The cells were incubated with subsequent addition of healing solution (2 mM CaCl2 ...
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bioRxiv - Synthetic Biology 2024Quote: ... 10 million induced cells from each track were washed and labelled for HA and c-Myc detection as described above and sorted on an S3e cell sorter (BioRad) to isolate cells that show HA detection ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... The gel was transferred to a 0.22 μm nitrocellulose membrane (AmershamTM Protran, G10600080) through Mini Trans-Blot Electrophoretic Transfer Cell (BioRad, 1703930) for 60 min at 100 V ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples from LNCaP cells used to measure luciferase activity (described in Fig. 4A) were loaded into 4% to 15% SDS-PAGE (Bio-Rad) and subjected to electrophoretic analysis and subsequent blotting ...
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bioRxiv - Cancer Biology 2021Quote: The prepared whole cell lysates were used to conduct gel electrophoresis followed by transfer to a polyvinylidene fluoride membrane (Bio-Rad). The membrane was blocked in 5% milk solution in Tween-PBS (PBS with 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Cell sorting was performed on the Bio-Rad Sorter S3e instrument with the Software ProSort 1.5 (Bio-Rad, Hercules, California, USA).
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bioRxiv - Cell Biology 2019Quote: ... IL-10 and MCP-1 in cell culture supernatants at 24 and 96 h post-infection on the Bioplex 200 (Bio-Rad). The Bioplex Manager 6.1 software was used for data analysis (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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bioRxiv - Cell Biology 2020Quote: ... 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies) and in Criterion cells (Bio-Rad) using respective adapters (LifeTechnologies) ...
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bioRxiv - Genetics 2021Quote: 5 to 10 million cells were collected and suspended in the Laemmli buffer and separated on an SDS-PAGE gel (Bio-RAD). Following transfer to nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in TOP10 competent cells (Themo Fisher Science) and were purified using a Nuvia IMAC Nickel-charged column (Bio-Rad) on an NGC Quest chromatography system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Normalized volumes of samples from the different growth time points were applied on SDS-PAGE gels (10% acrylamide) and transferred to a nitrocellulose membrane using Mini Trans-Blot cells (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was decanted and the cell pellet was washed twice with cold cPBS (PBS treated with Chelex-100 resin (Bio-Rad) for 2 h ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) or in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... 5.0 millions of stably transduced cells were electroporated with 50 μg plasmids by the Gene Pulser X cellTM Electroporation System (Bio-Rad) at 250 V and 1500 microfarads ...
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bioRxiv - Bioengineering 2020Quote: ... of the of the cell lysate were loaded and separated by SDS–PAGE before transfer to a nitrocellulose membrane (BioRad, #1620177). Membranes were incubated with primary mouse anti-EGFR antibody (BioLegend ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was transferred onto an Amersham Hybond-NX nylon membrane with a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) by applying 20 V for 1 h and then fixed using the EDC-mediated (1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Neuroscience 2019Quote: ... boiled for 1 min and an equivalent of ∼1.5 brains was immediately loaded onto a 10% acrylamide gel for SDS-PAGE at 80V (Mini-Protean Cell, BioRad, Hercules, CA). Separated proteins were blotted onto nitrocellulose membranes at 20 V for 6 minutes using an iBlot system (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: Gels loaded with whole-cell lysates or purified samples were Coomassie-stained using Bio-Safe™ Coomassie Stain (Bio-Rad Laboratories). For detection of iron-containing proteins ...
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bioRxiv - Microbiology 2019Quote: ... 250 µl packed cells were combined with 250 µl of 1 × Cytomix in a 2 mm transfection cuvette (Bio-Rad Laboratories). The precipitated and resuspended DNA was added to the cell suspension in the cuvette ...
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bioRxiv - Zoology 2019Quote: ... Equal amounts of total protein per sample were separated by SDS gel electrophoresis on a 12.5% polyacrylamide gel [39] using a Mini-PROTEAN II Electrophoretic Cell (Bio-Rad, USA). Wet-transfer of the proteins from the gel to a polyvinylidene difluoride transfer membrane (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... they were transferred from the gel to a PVDF membrane using a Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) and applying 250 mA ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were then washed three times with DI water and stained with 2 ml hematoxylin (Bio-Rad Laboratories, Hercules, CA, USA) for 15 min at room temperature ...
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bioRxiv - Synthetic Biology 2019Quote: ... Samples with thereby prepared extracts equivalent to ∼ 108 cells per lane were loaded on SDS-PAGE gels (Miniprotean system, Bio-Rad). Following electrophoresis ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: Total RNA from SIM-A9 cells with varying treatment conditions was reverse transcribed to cDNA libraries using the iScript cDNA Synthesis Kit (Bio-Rad) with a 1 μg RNA input ...
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bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
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bioRxiv - Microbiology 2020Quote: ... The gel was blotted on a PVDF membrane using a semi-dry transfer unit (Trans-Blot Semi-Dry Electrophotetic transfer cell, Bio-Rad) at 25V for 45 minutes ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was performed using the TaqMan Fast Advanced Cells-to-CT kit with the FX96 Real-Time PCR Detection System (Bio-Rad) with the TaqMan probes listed in table S15 ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... The solution was then pelleted to remove cell debris and the supernatant was mixed 1:1 with 2x Laemli sample buffer (Bio-Rad) and beta-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... Equal volumes (30 μg) of cell lysates from each condition were resolved by AnykD Mini-PROTEAN TGX precast gels (#456-9035, BIO-RAD) or 7.5% Mini-PROTEAN TGX precast gels (#456-8024 ...