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Citations for Bio-Rad :
3501 - 3545 of 3545 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: A multiplex biometric immunoassay containing fluorescent dyed microbeads was used to measure cytokines in the cell culture supernatant (Bio-Rad Laboratories, Hercules, CA, USA). The following cytokines were quantified ...
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bioRxiv - Cancer Biology 2019Quote: ... for approximately 45 min using the Mini-PROTEAN Tetra Cell and PowerPac™ 300 using tris-glycine SDS running buffer (all Bio-Rad, Watford, UK). Protein ladder SeeBlue™ Plus 2 Pre-stained Protein Standard (Invitrogen™ through Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2020Quote: The resuspended cDNA was divided into six aliquots and each aliquot was transformed into ElectroMax™ DH10B™ T1 phage resistant competent cells in a separate vial using a Gene pulsor Xcell™ electroporation system (Bio-Rad) as mentioned in the kit’s manual ...
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bioRxiv - Biochemistry 2020Quote: ... Native-SDS PAGE was carried out as follows: samples were subjected to a Bio-Rad Protean II xi slab cell (Bio-Rad, Richmond, CA, USA), with a discontinuous buffer system (Laemmli ...
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bioRxiv - Neuroscience 2022Quote: We prepared cellular extracts by a single-step lysis method.75 We suspended harvested cells in SDS-PAGE sample buffer [Laemmli sample buffer (Bio-Rad, Cat# 161-0737)] before boiling for 5 min ...
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bioRxiv - Immunology 2022Quote: ... Proteins were separated on 10%–12% SDS polyacrylamide gels by electrophoresis and transferred onto nitrocellulose membranes using a Trans-Blot SD Semi-Dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% skim milk for 1 h ...
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bioRxiv - Cell Biology 2019Quote: ... Separation in the second dimension was accomplished using an 10% SDS-PAGE in a Protean II xi 2-D Cell (Bio-Rad, Hercules, CA, USA) at 50 mA until the bromophenol blue reached the bottom of the gel ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Plant Biology 2020Quote: ... Proteins were transferred from polyacrylamide gel to polyvinylidine difluoride (PVDF) membranes as described by (Towbin et al., 1979) using a Trans-blot® electrophoretic transfer cell (Bio-Rad, city, country). The PVDF membrane with transferred proteins was blocked overnight at 4°C in blocking solution (5% (w/v ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentrations in cell lysates were determined by the Bradford method using the Bio-Rad protein assay dye reagent (Bio-Rad Laboratories, Hercules, CA) according to the instructions of the supplier.
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bioRxiv - Microbiology 2021Quote: ... 50 μl of cells were mixed with PCR products and transferred to a precooled 2 mm gap electroporation cuvette (Bio-Rad Laboratories, Hercules, CA). A BTX Gemini X2 instrument was used for electroporation with the following settings ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins or whole cell lysates were separated by SDS-polyacrylamide gel electrophoresis on a 4-20% gradient gel (Bio-Rad Laboratories, Hercules, CA) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Immunology 2020Quote: ... The cellular localization of EqPD-1-EGFP and EqPD-L1-EGFP was then confirmed using the ZOE Fluorescent Cell Imager (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2022Quote: ... 30 μl were loaded and resolved on 6% sodium dodecyl sulfate (SDS) gels and transferred onto nitrocellulose membranes with Mini-PROTEAN III and Mini Trans-Blot cells (Bio-Rad, Hercules, CA, USA), respectively ...
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bioRxiv - Microbiology 2022Quote: ... the red fluorescent intensities of the microchambers on each device were examined to determine the MIC using a fluorescent microscope (ZOE™ Fluorescent Cell Imager system; Bio-rad, Hercules, California). For slow growth samples where the MIC was not clear after 4 h incubation ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to PVDF transfer membrane (IPVH00010 Immobilon®-P; MilliporeSigmaTM, Burlington, USA) overnight at 10 mA in a cold room (Mini trans-blot cell; Bio-Rad, Hercules, USA). The membrane was blocked for non-specific sites and incubated with anti-HvHsfA6a in 1:2000 dilutions overnight at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Cell lysates were separated on an 8-12% SDS-polyacrylamide gel and blotted onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA), The membrane was incubated overnight at 4°C with primary Abs ...
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bioRxiv - Biochemistry 2023Quote: ... Total protein concentrations of the cell extracts were determined using the Bio-Rad Protein Assay Kit according to the manufacturer’s instructions (Bio-Rad Laboratories, Hercules, CA, USA) using BSA as standard.
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bioRxiv - Cell Biology 2023Quote: ... the protein was transferred to the 0.2 μm nitrocellulose membrane using a semi-dry transfer system (#1703940, Trans-Blot® SD Semi-Dry Transfer Cell, Bio-Rad, Hercules, CA, USA) for 55 min at 25 V using 1x transfer buffer (Pierce Western Blot Transfer Buffer 10x ...
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were obtained from MAYV- or UNAV-infected HMC3 or HBEC-5i cells in Laemmli buffer with 10% dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were fractionated in SDS-PAGE ...
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bioRxiv - Cancer Biology 2023Quote: IRX4_PEP1 transfected PC3 cell protein lysate was pre-cleared with 25 μL of SureBeads TM Protein G magnetic beads (BIO-RAD, Catalog number – 1614023) for 1 hour at 4 ºC and the beads were removed three times using a magnetic stand ...
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bioRxiv - Biophysics 2023Quote: ... then transferred onto PVDF membrane (Hybond-LFP, Amersham BioSciences, Little Chalfont, Buckinghamshire, United Kingdom) by using a semi-dry blotter (Trans-Blot Cell, Bio-Rad, Hercules, CA, USA). To differentiate the truncated product from T2 ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µg of each cell protein lysate sample was separated using a hand-cast 5-15% gradient Tris glycine gel (Bio-Rad, Protocol Bulletin 6201) using a custom made 25-well comb.
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bioRxiv - Synthetic Biology 2023Quote: ... 1 μg of dsDNA were added to the competent cells and then transferred to chilled 2 mm gap electroporation cuvettes (Bio-Rad Laboratories GmbH, Germany). Electroporation transformation was done with a single pulse at 1.8 kV ...
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bioRxiv - Developmental Biology 2023Quote: ... resolved by SDS-PAGE and transferred to PVDF membranes in a Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad, Hercules, CA, USA). Unspecific binding to membranes was prevented by blocking in 5% bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples containing 30 μg of total protein were separated by SDS-PAGE for 30 min at 270 V using 4–20% gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Bioengineering 2023Quote: ... was subjected to SDS-PAGE using a 4-20% Mini-PROTEAN TGX acrylamide gel on a Mini-PROTEAN Electrophoresis cell (Bio-Rad Laboratories, CA, USA). Electrophoretically separated proteins were transferred to Immobilon-P PVDF membrane (25V/RT/overnight) ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was used as cell extract for further experiments after determination of the protein concentration using Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Cell Biology 2020Quote: ... Insert DNA was derived from total RNA extracted from U2OS cells and then reverse transcribed using an iScript™ Reverse Transcription Supermix (Bio-Rad Laboratories, Hercules, CA). A 140 bp region of alpha satellite sequence from chromosome 4 and a 349 bp region of HSATII from chromosome 7 were independently cloned into pTargeT™ DNA backbone via StrataClone TA cloning (Agilent Technologies ...
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bioRxiv - Bioengineering 2019Quote: Free FAM NHS ester remaining after purification was quantified by polyacrylamide gel electrophoresis (PAGE) run according to the Laemmli protocol51 (adapted in BioRad Mini-PROTEAN Tetra Cell manual). Briefly ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were electrophoresed in a Bio-Rad Criterion TM Vertical Electrophoresis Cell at 90-120V for 1-2 hours and then transferred to a PVDF Transfer Membrane (#88518, Bio-Rad, Hercules, CA, United States) using the Bio-Rad Trans-Blot Turbo Transfer System ...
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bioRxiv - Microbiology 2020Quote: Cell lysates in 1X RIPA buffer were mixed with an equal volume of 2X Laemmli sample buffer (Bio-RAD, containing 50 µl/ml 2-mercaptoethanol) followed by boiling for 5 minutes ...
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bioRxiv - Plant Biology 2022Quote: The GFP and DAI-GFP plasmids were transiently introduced into onion epidermal cells using a helium-driven particle accelerator (PDS/1000; Bio-Rad Laboratories, Hercules, CA, USA). The particle bombardment experiments were performed as described by Arimura et al ...
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bioRxiv - Cancer Biology 2019Quote: ... Equal amounts of whole-cells and tissue extract (20-60 µg) were separated on 15% SDS-polyacrylamide gel and transferred onto nitrocellulose membranes (Bio-Rad Laboratories, Mississauga, ON, Canada). The membranes were first blocked with 5% milk [w/v] in TBS/0.05% Tween 20 [v/v] for 60 min at room temperature and subsequently blotted overnight at 4º C with primary antibodies (see Table 2) ...
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bioRxiv - Neuroscience 2019Quote: ... or BIC (20 μM) were added to the culture and cells were harvested with a 1:1 mixture of 2X Laemmeli buffer (BioRad, 1610737, 1:20 beta-mercaptoethanol) and radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Genetics 2020Quote: ... Sections of the central retina (containing the optic nerve) were examined and photographed under the ZOE™ Fluorescent Cell Imager (Bio-Rad, Hercules, CA, USA). The ImageJ software was used for measuring the thickness of the retinal layers at 200 μm intervals ...
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bioRxiv - Immunology 2021Quote: ... For T84 cells: 250 ng of total RNA was reverse transcribed into cDNA using the iScript™ cDNA Synthesis kit (BioRad Laboratories, Catalog number 1708891). The reaction contained a mixture of 1 μl Reverse Transcriptase ...
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bioRxiv - Microbiology 2022Quote: ... samples were separated on 8-12% SDS-PAGE gels and transferred to nitrocellulose membranes using semi-dry transfer (BioRad Trans-Blot Semi-Dry Transfer Cell). Membranes were blocked in 5% non-fat milk in Tris-buffered saline with 0.1% Tween-20 (TBST ...
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bioRxiv - Neuroscience 2023Quote: ... from cells or brain tissue were separated on SDS polyacrylamide gels (4–15% Mini-PROTEAN® TGX Stain-Free™ gels, Bio-Rad, Hercules, USA) and transferred onto a membrane (nitrocellulose or PVDF ...
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bioRxiv - Cancer Biology 2023Quote: Proteins from cell extracts were separated by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) on 4-20% gradient gels (Bio-Rad #4561095; Hercules, CA, USA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Cancer Biology 2023Quote: ... the formazan dye produced by viable cells was quantified by measuring the absorbance at 490 nm on the Bio-Rad Model 680 microplate reader (Bio-Rad Laboratories, Hercules, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... The same amount of cell lysates or immune precipitates was resolved by 10% SDS-PAGE and transferred to the PVDF membrane (Bio-Rad Laboratories, Hercules, CA, USA) by electrophoresis ...
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bioRxiv - Systems Biology 2021Quote: ... plates were centrifuged for 10 min at 2000g and heated at 95C for 30 min (cell culture) or 60 min (tissue) in a thermal cycler (Biorad S1000 with 384-well reaction module) at a constant lid temperature of 110 °C ...
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bioRxiv - Cancer Biology 2020Quote: ... blots were developed using an ECL Ultra kit (New Cell & Molecular Biotech Co. Ltd, China) and images were captured using a ChemiDocTM XRS + Imager (Bio-Rad Laboratories, Inc., Hercules, CA, USA).