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Citations for Bio-Rad :
3451 - 3500 of 3545 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Protein lysates from SH-SY5Y cells were quantified (bicinchoninic acid protein assay, Pierce) and then electrophoresed in 4—12% Mini-PROTEAN® TGX™ Precast Gels (Bio-Rad) in 1X TGS Tris/Glycine/SDS Buffer (10X TGS Buffer ...
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bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
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bioRxiv - Bioengineering 2021Quote: ... All plasmids in this study have been transformed into cells using electroporation with the Bio-Rad Micropulser™ (Bio-Rad Laboratories, Hercules, CA) and were created using Gibson Assembly[51] using the Gibson Assembly Master Mix (New England Biolabs ...
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bioRxiv - Biophysics 2021Quote: ... loaded them into polyacrylamide gel wells and ran them for approximately 45 minutes at 190 V in a mini-PROTEAN tetra vertical electrophoresis cell (Bio-Rad Laboratories, Inc). We transferred the protein bands onto PVDF membranes (170-4156 ...
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bioRxiv - Biochemistry 2020Quote: ... EV proteins were electrophoresed on a 12.5 % polyacrylamide gel (50V, 30 min) to obtain a single band (Mini-Protean II electrophoresis cell, BioRad, Marnes-la-Coquette, France). The gel was stained with Coommassie Blue and the single band of proteins was excised and subjected to in-gel digestion with bovine trypsin (Roche Diagnostics GmbH ...
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bioRxiv - Microbiology 2019Quote: ... Purified protein was denatured and separated by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) in a Criterion™ Cell and transferred to a nitrocellulose membrane in a Criterion™ Blotter (Bio-Rad) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Equal amounts of protein from cell lysates were separated by SDS PAGE and transferred onto a nitrocellulose membrane by a semi-dry transfer method (Trans Blot® SD Semi-Dry Transfer cell, Bio-Rad, USA). Membranes were blocked with 5 % milk solution before incubation with primary antibodies at 1:1000 dilution unless otherwise stated ...
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bioRxiv - Neuroscience 2020Quote: 15 μg of total protein extract from purified proteins were separated by native electrophoresis using a Tetra cell (Bio-Rad; Hercules, CA, USA), in 12% polyacrylamide separation gel and a 4% polyacrylamide stacking gel in non-denaturing conditions ...
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bioRxiv - Immunology 2019Quote: Intestinal epithelial cells lysate (20 µg per lane) were separated by SDS-PAGE through 4%-20% Mini-PROTEAN® TGX™gel (BIO-RAD), transferred to nitrocellulose membranes ...
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bioRxiv - Biochemistry 2020Quote: Protein lysate from DENV infected and non-infected Huh7 cells (10 µg of protein as measured using Bio-Rad protein assay dye reagent) was aliquoted and purified using methanol/chloroform precipitation ...
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bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: The protein extracts from the cells (40 μg) were subjected to electrophoresis in SDS-PAGE on 4–15% precast protein gels (Bio-Rad, Hercules, CA), separated and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: 2 μg of total protein derived from meiotic or post-meiotic germ cell populations was loaded onto a Mini-PROTEAN TGX gel (Bio-Rad, 456-8093) and separated by SDS-PAGE for 1 hour at 150V ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg of ΔORF3-E mNG RNA and 20 μg of N-gene RNA were electroporated into 8×106 Vero-ORF3-E cells using the Gene Pulser XCell electroporation system (Bio-Rad, Hercules, CA) at a setting of 270V and 950 μF with a single pulse ...
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bioRxiv - Microbiology 2022Quote: ... full-length S protein samples were prepared from cell pellets and resolved in 4-15% Mini-Protean TGX gel (Bio-Rad, Hercules, CA) and transferred onto PVDF membranes ...
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Genetics 2022Quote: ... and transferred to an Immun-Blot™ PVDF membrane using a Trans-Blot® SD semi-dry transfer cell (BioRad Inc., CA, USA). Membranes were blocked with 5% non-fat dry milk in PBST for 2h ...
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bioRxiv - Microbiology 2024Quote: ... Then 300 ng of plasmids were transformed into 100 μl of competent cells using the bacteria program on the MicroPulser Electroporator (Bio-Rad, CA, USA). After 1 h recovery culture at 37°C in 500 μl of LB ...
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bioRxiv - Microbiology 2022Quote: ... The PCR products (700 ng) were introduced into electrocompetent cells of JW0452 carrying pKD46 by electroporation using a Gene Pulser XcellTM (BioRad, Hercules, CA, USA). The occurrence of recombination at the expected site was confirmed by PCR using primers CAT-584 and BW25113_2815723f (Table 2) ...
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bioRxiv - Genetics 2024Quote: ... The percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7; Bio-Rad, Hercules, CA, USA) using the following reaction mixture ...
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bioRxiv - Cancer Biology 2023Quote: ... 10-20 µl of the cell lysates were separated on 4-15% Mini-PROTEAN® TGX™ Stain-FreeTM Protein Gels from Bio-Rad and transferred to methanol-soaked polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: Total protein lysates were prepared from the cells followed by protein quantification using the protein DC assay kit (Bio-Rad, Cat # 5000-001) as described previously (28) ...
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bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... cervicornis sperm samples from 2015 were brought to a final concentration of 3 x 107 cells ml-1 after the addition of Cell Suspension Buffer and 2% agarose using the Bio-Rad CHEF Genomic DNA Plug Kits (Bio-Rad, Hercules, CA). Genomic DNA plugs were processed according to the manufacturer’s protocol and stored at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... 5 µg in vitro transcripts were transfected into 5,000,000 cells resuspended in 400 µL cytomix containing 2 mM ATP and 5 mM glutathione using a Gene Pulser Electroporator (Bio-Rad, Munich, Germany). Cells were immediately transferred into 12 mL fresh medium and seeded in 96-well plates at density of 20,000 cells/well for replication assays and 12 mL/10cm dish for virus production.
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration in cell lysates were measured using a RC DC Protein Assay Kit (cat. no. 50000119; Bio-Rad Laboratories, Hercules, CA, USA). Immunoblotting was carried out using standard procedures ...
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bioRxiv - Cancer Biology 2023Quote: ... The cell suspension then was divided equally and heated for 3 min at indicated temperatures on T100™ Thermal Cycler (Bio-Rad Laboratories). Cells were snap-frozen and thawed twice and centrifuged at 17.000 g for 15 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resultant homogeneous single-cell suspensions were embedded in agarose and transferred into plug molds (Bio-Rad CHEF Mammalian Genomic DNA plug kit). Plugs were allowed to solidify at 4°C and were then incubated with Proteinase K (QIAGEN ...
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bioRxiv - Cell Biology 2023Quote: ... 10 µL of the first PCR amplification product was then used for analysis on a 2% ethidium bromide-stained agarose gel (120 minutes at 80V in a Mini-Sub® Cell GT gel chamber connected to a PowerPac™ HC (Bio-Rad) power supply ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspension was transferred to a 0.2 cm gap cuvette and immediately electroporated by GENE PULSER II (Bio-Rad, Hercules, CA, USA) at 0.45 kV and 50 μF ...
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bioRxiv - Cell Biology 2022Quote: ... Supernatants were collected and stored at −80□°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
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bioRxiv - Bioengineering 2023Quote: ... at 100V with a constant current for 60 mins at 4°C in a Bio-Rad Mini-Transfer Cell (Bio-Rad, Cat# 1703930) according to the manufacturer’s specifications ...
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bioRxiv - Molecular Biology 2023Quote: ... or both at the University of Georgia (Athens, Georgia) Flow Cytometry Core Center using a Bio-Rad S3e cell sorter (Bio-Rad, Irvine, California).
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bioRxiv - Synthetic Biology 2024Quote: ... Percentage of targeted alleles within a cell population was measured with a Bio-Rad QX200 ddPCR machine and QuantaSoft software (v.1.7; Bio-Rad, Hercules, CA, USA) using the following reaction mixture ...
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bioRxiv - Developmental Biology 2020Quote: ... and cells resuspended in PBS were transfected with 75μg DNA constructs (Gene Pulser Xcell System; Bio-Rad; 250 V, 500 μF, 0.4-cm cuvettes). Cells were subsequently plated on MEF feeder layers (DR4 MEFs for puromycin or neomycin selection ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were separated in 12.5 % sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE; 100V for 90 minutes) in Mini-PROTEAN Tetra Vertical Electrophoresis Cell (Bio-Rad, Hercules, CA, USA) and transferred into polyvinylidene difluoride (PVDF ...
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bioRxiv - Physiology 2019Quote: ... Second-dimension sodium dodecyl sulphate-polyacrilamide gel electrophoresis (SDS-PAGE) was done on 12.5% Tris-HCL Precast Gels using a Criterion electrophoresis cell (Bio-Rad Laboratories, Hercules CA, USA), run at 80V for 20 min before switching to 100V for approximately 2 h ...
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bioRxiv - Bioengineering 2019Quote: ... Equal volumes of the cell suspension (RT) and the agarose solution (75°C) were mixed and immediately transferred to plug molds (Bio-Rad Laboratories, Cat. # 1703713), ∼100 μl mixture per plug ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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bioRxiv - Molecular Biology 2021Quote: ... the cell lysates were separated by 15% SDS-polyacrylamide gel electrophoresis and electro-blotted onto a Immun-Blot PVDF Membrane using a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 2% skimmed milk and probed with antibodies ...
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bioRxiv - Plant Biology 2020Quote: ... or self-made gel and subjected to a sodium dodecyl sulfate-polyacrylamide gel (SDS-PAGE) using a Mini-PROTEAN® Electrophoresis cell (Biorad, Cat No 1658002EDU). Gels were run at 130 V for approx ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Cancer Biology 2021Quote: Separation of 20 μg or 50 μg protein from cell lysate was performed on a 10% Mini-PROTEAN® TGX™ gel (BioRad, Portland, ME) and subsequently transferred onto Immunoblot PVDF 0.45μm membranes (BioRad ...
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bioRxiv - Cell Biology 2022Quote: ... Equal concentrations of samples (15μg for whole cell lysates and 5μg for nuclear fractions) were resolved on Criterion gels (Bio-Rad, catalog number 5671093 and 5671094) and transferred to nitrocellulose (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were obtained from mock-or MAYV-infected HeLa cells or HDFs that were treated or untreated with PIM kinase inhibitors in Laemmli buffer with 10% Dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were separated in SDS-PAGE ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were solubilized in Laemmli sample buffer and heated at 95°C for 10 min to denature proteins and separated via SDS-PAGE for 40–60 min at 270 V using 4–20% stain-free gradient gels on Criterion electrophoresis cell (Bio-Rad Laboratories, Richmond, CA). Thereafter ...
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bioRxiv - Cancer Biology 2022Quote: Equal amounts of cell lysate were resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Blocking was performed for 60 min with 5% nonfat dry milk in Tris-buffered saline and Tween 20 ...
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bioRxiv - Neuroscience 2021Quote: ... QPCR was performed to determine Piezo2 knockdown effects by transfection of piezo2-shRNA into N2A cells using IQ Syber Green supermix (Bio-rad, Hercules, CA, USA) on a Bio-rad CFX96 Real-time PCR Machine and specific intron-spanning primers to quantify the cDNA levels of Piezo2 (Table 1) ...
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bioRxiv - Cell Biology 2019Quote: Sequence-confirmed constructs were linearized and transformed into the macronucleus of WT cells by biolistic bombardment using a PDS-1000 particle bombarder (Bio-Rad, Hercules, CA, USA) (Bruns and Cassidy-Hanley ...