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Citations for Bio-Rad :
3151 - 3200 of 3545 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... IL-10 and MCP-1 in cell culture supernatants at 24 and 96 h post-infection on the Bioplex 200 (Bio-Rad). The Bioplex Manager 6.1 software was used for data analysis (Bio-Rad) ...
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bioRxiv - Cell Biology 2020Quote: ... 2 mM L-glutamine and counted in the presence of Trypan Blue using the TC20™ Automated Cell counter (Bio-Rad) (24h floating cell count) ...
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bioRxiv - Cell Biology 2020Quote: ... 2-20 μg protein per sample were separated for 2-4 h at 60 – 100 V using 8 % 26 well NuPAGE Bis-Tris Midi gels in MOPS or MES buffer (LifeTechnologies) and in Criterion cells (Bio-Rad) using respective adapters (LifeTechnologies) ...
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bioRxiv - Immunology 2021Quote: ... Macrophages and T cells in aortic root were stained by incubating aortic root cross-sections with the primary antibodies to CD68 (Bio-Rad) and CD3 (Abcam) ...
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bioRxiv - Genetics 2021Quote: 5 to 10 million cells were collected and suspended in the Laemmli buffer and separated on an SDS-PAGE gel (Bio-RAD). Following transfer to nitrocellulose membrane (GE Healthcare) ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were expressed in TOP10 competent cells (Themo Fisher Science) and were purified using a Nuvia IMAC Nickel-charged column (Bio-Rad) on an NGC Quest chromatography system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... Normalized volumes of samples from the different growth time points were applied on SDS-PAGE gels (10% acrylamide) and transferred to a nitrocellulose membrane using Mini Trans-Blot cells (Bio-Rad Laboratories Ltd. ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins on the gels were transferred onto PVDF membrane using BioRad Mini Trans-Blot system (BioRad, Mini Trans-Blot® Cell). The membranes were washed with pure water and blocked with 5% skimmed milk in 0.1% Tween-20/PBS (PBST ...
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bioRxiv - Microbiology 2020Quote: ... The supernatant was decanted and the cell pellet was washed twice with cold cPBS (PBS treated with Chelex-100 resin (Bio-Rad) for 2 h ...
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bioRxiv - Systems Biology 2022Quote: ... Drugs were then added to the plates and protein was harvested after 2 hours using the Bio-Plex Pro Cell Signaling Reagent Kit (BioRad 171304006M) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were transferred onto nitrocellulose membranes (Amersham Protran) for 30 min at 15V with a Trans-Blot SD semidry transfer cell (Bio-Rad) or in transfer buffer (25 mM Tris ...
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bioRxiv - Molecular Biology 2020Quote: ... 5.0 millions of stably transduced cells were electroporated with 50 μg plasmids by the Gene Pulser X cellTM Electroporation System (Bio-Rad) at 250 V and 1500 microfarads ...
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bioRxiv - Bioengineering 2020Quote: ... of the of the cell lysate were loaded and separated by SDS–PAGE before transfer to a nitrocellulose membrane (BioRad, #1620177). Membranes were incubated with primary mouse anti-EGFR antibody (BioLegend ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was transferred onto an Amersham Hybond-NX nylon membrane with a Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) by applying 20 V for 1 h and then fixed using the EDC-mediated (1-ethyl-3-(3-dimethylaminopropyl ...
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bioRxiv - Neuroscience 2019Quote: ... boiled for 1 min and an equivalent of ∼1.5 brains was immediately loaded onto a 10% acrylamide gel for SDS-PAGE at 80V (Mini-Protean Cell, BioRad, Hercules, CA). Separated proteins were blotted onto nitrocellulose membranes at 20 V for 6 minutes using an iBlot system (Invitrogen ...
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bioRxiv - Synthetic Biology 2019Quote: Gels loaded with whole-cell lysates or purified samples were Coomassie-stained using Bio-Safe™ Coomassie Stain (Bio-Rad Laboratories). For detection of iron-containing proteins ...
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bioRxiv - Microbiology 2019Quote: ... 250 µl packed cells were combined with 250 µl of 1 × Cytomix in a 2 mm transfection cuvette (Bio-Rad Laboratories). The precipitated and resuspended DNA was added to the cell suspension in the cuvette ...
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bioRxiv - Zoology 2019Quote: ... Equal amounts of total protein per sample were separated by SDS gel electrophoresis on a 12.5% polyacrylamide gel [39] using a Mini-PROTEAN II Electrophoretic Cell (Bio-Rad, USA). Wet-transfer of the proteins from the gel to a polyvinylidene difluoride transfer membrane (GE Healthcare ...
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bioRxiv - Cancer Biology 2019Quote: ... they were transferred from the gel to a PVDF membrane using a Mini Trans-Blot® Electrophoretic Transfer Cell (Bio-Rad) and applying 250 mA ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were then washed three times with DI water and stained with 2 ml hematoxylin (Bio-Rad Laboratories, Hercules, CA, USA) for 15 min at room temperature ...
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bioRxiv - Synthetic Biology 2019Quote: ... Samples with thereby prepared extracts equivalent to ∼ 108 cells per lane were loaded on SDS-PAGE gels (Miniprotean system, Bio-Rad). Following electrophoresis ...
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Transcriptomic and morphological response of SIM-A9 mouse microglia to carbon nanotube neuro-sensorsbioRxiv - Bioengineering 2020Quote: Total RNA from SIM-A9 cells with varying treatment conditions was reverse transcribed to cDNA libraries using the iScript cDNA Synthesis Kit (Bio-Rad) with a 1 μg RNA input ...
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bioRxiv - Genomics 2020Quote: ... were seeded per well in 6-well plates (2 ml per well). 24 hrs later cells were transfected with siRNA (Supp. Table 9) using SilentFect (Bio-Rad) in biological triplicates ...
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bioRxiv - Microbiology 2020Quote: ... The gel was blotted on a PVDF membrane using a semi-dry transfer unit (Trans-Blot Semi-Dry Electrophotetic transfer cell, Bio-Rad) at 25V for 45 minutes ...
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bioRxiv - Genomics 2021Quote: ... qRT-PCR was performed using the TaqMan Fast Advanced Cells-to-CT kit with the FX96 Real-Time PCR Detection System (Bio-Rad) with the TaqMan probes listed in table S15 ...
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bioRxiv - Microbiology 2021Quote: ... anti-FLAG (Cell Signalling Technologies, 2908S), anti-SARS-CoV-2-ORF8 (MRCPPU, University of Dundee, DA088, [37]) and anti-Tubulin (BioRad, MCA77G). Donkey anti-rabbit IRDye 800 and goat anti-rat IRDye 680 (Li-cor Biosciences ...
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bioRxiv - Microbiology 2021Quote: ... The solution was then pelleted to remove cell debris and the supernatant was mixed 1:1 with 2x Laemli sample buffer (Bio-Rad) and beta-mercaptoethanol ...
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bioRxiv - Neuroscience 2021Quote: ... Equal volumes (30 μg) of cell lysates from each condition were resolved by AnykD Mini-PROTEAN TGX precast gels (#456-9035, BIO-RAD) or 7.5% Mini-PROTEAN TGX precast gels (#456-8024 ...
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bioRxiv - Neuroscience 2020Quote: ... N2a cells were harvested at the same time points for reverse-transcriptase (RT) PCR generation using iScript cDNA Synthesis kit (Bio-Rad), and quantitative PCR (QPCR ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples (20 µg/lane) were electrophoresed on a 6–12% SDS polyacrylamide gel in a Mini-PROTEAN Tetra Cell (Bio-Rad) and subsequently transferred to a nitrocellulose membrane (Sartorius AG ...
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bioRxiv - Cancer Biology 2020Quote: ... and viability was monitored regularly using the Trypan Blue exclusion test on a TC20 automated cell counter (Bio-Rad, Hercules, CA). Once the cells reached ∼95% confluence ...
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bioRxiv - Cancer Biology 2020Quote: ... and growth factors in culture supernatants of control or R1881 treated LNCaP cells was determined using the Bioplex Pro Human Cytokine 17-plex assay system (Bio-Rad) according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were individually partitioned into the droplets together with beads with barcodes and RT mix using ddSEQ Single-Cell Isolator (Bio-Rad). The cell lysis and RT took place in each droplet ...
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bioRxiv - Neuroscience 2020Quote: ... Equal amount of proteins from each lysate were subjected to polyacrylamide gel (4-10 %) electrophoresis (PAGE) in Mini-PROTEAN Tetra cell apparatus (Bio-Rad). PAGE separated proteins were blotted onto a PVDF membrane in a semi-dry Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Microbiology 2021Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 μg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates were normalised after Bradford protein quantification and resolved on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad), transferred to nitrocellulose and blocked in 5% milk in PBS-T (PBS + 0.05% Tween 20) ...
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bioRxiv - Microbiology 2020Quote: ... The proteins of an 0.1 OD600 equivalent quantity of cells were separated by SDS-polyacrylamide denaturing gel electrophoresis using precast TGX 4-15% gradient gels (Bio-Rad). The proteins were then transferred to a 0,2µm nitrocellulose membrane using Trans-Blot Turbo RTA Transfer Kit ...
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bioRxiv - Cell Biology 2020Quote: Extracellular medium and cell lysates were spotted on nitrocellulose membranes (0.45 μm) using Bio-Dot Microfiltration Apparatus (Bio-Rad, California, USA) (manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... ∼500bp fragments of sRNA factors were amplified from cDNA of ISE6 cells using primers listed in Table S1 using iProof High- Fidelity Master Mix (Bio-Rad) and the amplicons were treated with XhoI to insert them in the XhoI site of pLitmus28i (NEB) ...
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bioRxiv - Genetics 2022Quote: Template cDNA pools were generated from 1 µg RNA using cells grown under standard glucose conditions (as above) by using iScript (Bio-Rad) RT with first-strand synthesis priming from a mixture of random hexamers and oligo-dT ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates were resolved by SDS-PAGE (Sodium Dodecyl Sulfate PolyAcrylamide Gel Electrophoresis) using 4-20% precast gel (Biorad, Hercules, CA). Proteins were transferred by semi-dry transfer onto a PVDF (polyvinylidene fluoride ...
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bioRxiv - Cell Biology 2022Quote: ... 192 mM glycine and 20% of methanol) for 15 min before assembly in the Mini Trans-Blot transfer cell (BioRad 1703935). The transfer was done at 4°C for 16 h at 110 mA constant current ...
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bioRxiv - Biochemistry 2022Quote: ... and purified by vertical gel electrophoresis on a 6% (60:1 Acrylamide/Bisacrylamide) native gel column using a 491 Prep Cell (Bio-rad) run at 10W and 4°C ...
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bioRxiv - Microbiology 2022Quote: ... 20% (v/v) methanol) at a constant amperage of 300 mA for 90 min using a Mini Trans-Blot Cell (Bio-Rad). The membranes were incubated in NRA blocking buffer (INNOVAX ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell lysates (10-50 μg) were subjected to SDS-polyacrylamide gel electrophoresis on a 4-20% precast gradient gels (BioRad, 5678094) and proteins were transferred to nitrocellulose membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2021Quote: ... Equivalent protein samples (cell lysates and DRG fractionized membrane and cytosol) were size separated using 10% or 4-20% SDS-PAGE gels (Bio-Rad), transferred to Immun-Blot PVDF membranes (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: Total protein extracts (5 × 106 cells) were separated by SDS-PAGE (10%) and immunoblotted on TransBlot Turbo Midi-size PVFD Membranes (Bio-Rad) [55] ...
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bioRxiv - Neuroscience 2020Quote: ... 40 μg protein of N13 cell lysates was loaded to 7.5% Mini-PROTEAN® TGX Stain-Free™ Protein Gels (BioRad) and transferred to nitrocellulose membrane using the Trans-Blot® Turbo™ RTA Mini Transfer Kit (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... Flow cytometric staining and analysis was done as described [8] and acquired on ZE5 cell analyzer (Bio-Rad, Hercules, CA, USA). Analysis was performed using Flow Jo software.
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bioRxiv - Biochemistry 2020Quote: ... steroid concentrations or their ratios were normalized to the total protein content of the incubations as determined in the supernatant after cell lysis using the DC Protein Assay (Bio-Rad).