Labshake search
Citations for Bio-Rad :
3251 - 3300 of 3545 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... whole cell lysates were incubated at 100°C for 10 minutes in 50 μl SDS loading buffer (950 μl Bio-Rad® Laemmli buffer ...
-
bioRxiv - Immunology 2021Quote: The total proteins extracted from A549 cells were fractionated in a DS-Sepharose column with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
-
bioRxiv - Immunology 2021Quote: The total proteins extracted from HFL1 cells were fractionated on DS-Sepharose columns with a BioLogic Duo-Flow system (Bio-Rad). About 40 mg of proteins in 40 ml of 10 mM phosphate buffer (pH 7.4 ...
-
bioRxiv - Immunology 2021Quote: ... Cell lysates were separated on 8-12% SDS-PAGE gel and transferred onto a PVDF membrane (Bio-Rad, Hercules, CA, USA). The membrane was incubated overnight with the respective primary antibodies at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... The protein contents of 100-µl cell samples were measured using the Bio-rad Protein Assay kit (Bio-rad, CA, USA). Uptake rates were calculated from retained activity and total added activity and expressed in pmol amino acid µg-1 of protein.
-
bioRxiv - Neuroscience 2021Quote: ... Gels were run for 2 hours and 10 min at 80V and transferred using the Trans-Blot SD Semi-Dry Transfer cell system (Bio-Rad). After transfer the LI-COR Revert 700 Total Protein Stain (LI-COR ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
-
bioRxiv - Microbiology 2020Quote: ... 20 μg of total cell lysates were resolved using electrophoresis on SDS-PAGE gels and transferred onto nitrocellulose membrane (Bio-Rad). The membrane was blocked in 5% skimmed milk in Tris-buffered saline with 0.05% Tween 20 (TBST ...
-
bioRxiv - Neuroscience 2021Quote: ... adult testes or hippocampus or cell pellets were homogenized in RIPA buffer and the protein concentration was determined using a Bradford Assay kit (Bio-Rad). For blot analysis ...
-
bioRxiv - Immunology 2022Quote: Synchronous stimulation of the RIG-I pathway in A549- or HepG2-based cell lines was carried out using the Gene Pulser Xcell modular electro-transfection system (Bio-Rad) and a ShockPod™ cuvette chamber (Bio-Rad) ...
-
bioRxiv - Neuroscience 2022Quote: Cell lysates prepared after sorting GFP+ cells into single tube were used in RT-qPCR analysis using SingleShot Two-Step RT-qPCR reagents (Bio-Rad) according to manufacture instruction ...
-
bioRxiv - Molecular Biology 2022Quote: ... The mixture was transferred to a pre-chilled 2 mm gap electroporator cuvette and cells were electroporated using the EC2 program (MicroPulser, Bio-Rad). Cells were then suspended in 3 ml 1/4 TSB (at room temperature ...
-
bioRxiv - Bioengineering 2022Quote: ... and run on the gels at 160 volts for 60 min in 1X TBE in a Mini-PROTEAN Tetra Cell (Bio-Rad). The gels were stained in 1X TBE containing 1X SYBR Safe (Thermo Fisher ...
-
bioRxiv - Biophysics 2022Quote: ... 5 µL of the soluble fractions of cell lysates were run on a 4-20% TGX Mini-Protean gradient gel (Bio-Rad) and stained using Gelcode Blue (Thermo Scientific) ...
-
bioRxiv - Cell Biology 2022Quote: ... Proteins from equivalent numbers of cells were then separated by SDS-PAGE and transferred to a nitrocellulose or PVDF membrane using Trans-Blot Turbo Transfer System or Mini Trans-blot cells (Bio-Rad). Membranes were blocked in 5% milk in TBST (137 mM NaCl ...
-
bioRxiv - Cancer Biology 2022Quote: ... 0.1% SDS) and transferred onto nitrocellulose membranes (Protran) with a Trans-Blot SD semi-dry or wet transfer cell (Bio-Rad). Proteins of interest were probed using the appropriate primary and Horse Radish Peroxidase (HRP)-conjugated secondary antibodies (Jackson Immunoresearch) ...
-
bioRxiv - Genomics 2022Quote: ... The nuclei pellet was resuspended in 1mL of cold DPBS followed by the quantification of nuclei using a Biorad TC20 Automated Cell Counter (Biorad #1450102).
-
bioRxiv - Microbiology 2022Quote: ... ∼2 μg purified PCR products were electroporated into 50 μL electrocompetent cells at 1.75 kV using 0.1 cm cuvettes (Bio-Rad cat. 1652089) and a MicroPulser electroporator (Bio-Rad cat ...
-
bioRxiv - Microbiology 2022Quote: ... The pellet obtained was dissolved in 10 μL of 20 mM Tris-HCl (pH 7.2) and 10 μL of Laemmli sample buffer (Mini-PROTEAN® Tetra Cell-Bio-Rad). The solubilized proteins were heated (95 °C ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 1 μg purified linear PCR products were added to the cell mixture and proceeded electro-transformation (Gene Pulser Xcell system (Bio-Rad) and Gene Pulser/Micropulser Electroporation Cuvettes ...
-
bioRxiv - Synthetic Biology 2022Quote: ... Obtained soluble and insoluble fraction (pellet of cell debris) was analyzed by SDS-PAGE using 4–20% Mini-PROTEAN TGX Precast Protein Gel (Bio-Rad) and Precision Plus Protein Dual Color Standard (Bio-Rad).
-
bioRxiv - Plant Biology 2023Quote: ... The recombinant plasmid was coated with 0.6 μm gold particles (1 μg DNA per 10 mg gold) and then introduced into onion epidermal cells using the particle bombardment method (PDS-1000/He particle delivery system, Bio-Rad). The epidermis was incubated on MS medium in darkness at room temperature (20–25 °C ...
-
bioRxiv - Plant Biology 2022Quote: ... Eight microliters of the micro-particle suspension (30 μl) were used for transformation of epidermal onion cells using the PDE-1000He particle gun (Bio-rad). Before transformation ...
-
bioRxiv - Microbiology 2023Quote: ... with 10 μg of plasmid DNA expressing LpAsp-GFP or LpCht-GFP as well as pTrex-n-eGFP using a Gene Pulser X cell electroporator (Bio-Rad) and cuvette (2 mm gap) ...
-
bioRxiv - Plant Biology 2023Quote: ... tabacum) BY-2 suspension-cultured cells and biolistic bombardment using a PDS-1000/He biolistic particle delivery system (Bio-Rad Laboratories) were performed as previously described (Banjoko and Trelease ...
-
bioRxiv - Molecular Biology 2022Quote: ... using 10 mM CAPS buffer (3-[cyclohexylamino]-1-propanesulfonic acid [pH 11]) in a Mini Trans-Blot Electrophoretic Transfer Cell tank (Bio-Rad) according to protocol provided by the manufacturer ...
-
bioRxiv - Molecular Biology 2022Quote: ... A final concentration of 0.1 μg/mL DAPI was then added for 10 mins and digital images were captured using ZOE Fluorescent Cell Imager (Bio-Rad). For negative controls ...
-
bioRxiv - Molecular Biology 2022Quote: ... 25 µg total RNA each from WT and rad6Δ cells was resolved on 15% TBE-Urea polyacrylamide gel (Bio-Rad, #3450091). The RNAs were then transferred onto positively charged nylone membrane (Sigma ...
-
bioRxiv - Microbiology 2022Quote: ... 30 μg of whole-cell lysate or IP elutions were resolved on 4 to 15% mini-PROTEAN TGX gels (Bio-Rad). Transfers to PVDF membranes (Bio-Rad ...
-
bioRxiv - Microbiology 2022Quote: All native electrophoresis gels were run in a Tetra Vertical Electrophoresis Chamber (Mini-PROTEAN® Tetra Cell, Bio-Rad, CA, USA). Recombinant PNPase (100 pmols ...
-
bioRxiv - Cell Biology 2022Quote: ... The resulting lysate was resolved on SDS-PAGE gel and protein was blotted to nitrocellulose membrane (Li-COR #926-31902) with mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for an hour in Odyssey Blocking Buffer (TBS ...
-
bioRxiv - Immunology 2022Quote: ... Even amounts of each protein sample were separated on a 4–16% Mini-PROTEAN TGX Stain-Free Precast Gels using Mini-PROTEAN Tetra Cell electrophoresis system (Bio-Rad). Upon completion of electrophoresis ...
-
bioRxiv - Systems Biology 2022Quote: ... Equal amounts of each protein sample were separated on 4% to 16% Mini-Protean TGX stain-free precast gels using the Mini-Protean Tetra cell electrophoresis system (Bio-Rad). Upon the completion of electrophoresis ...
-
bioRxiv - Biophysics 2022Quote: ... Both this sample and the total cell protein sample were mixed 1:1 with 2x loading buffer: 50% 4x Laemmli Sample Buffer (Bio-Rad), 30% 100 mM DTT ...
-
bioRxiv - Cell Biology 2022Quote: ... Cells were grown at 30°C for 3-6 days and images captured using a Chemidoc XRS+ imager (BioRad, Hercules, CA). All images were evenly adjusted in Photoshop (Adobe Systems Incorporated ...
-
bioRxiv - Molecular Biology 2024Quote: Cell cultures at the log phase of growth were collected and checked for cell number and viability using TC20 automated counter (Bio-Rad). Samples with >90 % viability were processed in order to prepare single cell suspensions and diluted into the target numbers plus the multiplet rate (1000 cells plus 0.8 % per library) ...
-
bioRxiv - Bioengineering 2023Quote: ... For detection, we used an anti-rabbit HRP-coupled secondary antibody (1:5000, Cell Signalling) and Western ECL substrate (#1705061, Bio-Rad. As a loading control ...
-
bioRxiv - Molecular Biology 2024Quote: ... Total protein content of cell free BALF was determined using the DC Protein Assay Kit (Cat # 5000111; BioRAD, Inc, Hercules CA) with bovine serum albumin as a standard according to the manufacturer’s instructions.
-
bioRxiv - Molecular Biology 2024Quote: ... a total of 20 µg whole cell lysate was resolved in a 10 % precast Mini-PROTEAN TGX Stain-Free gel (Bio-Rad). At the end of the run ...
-
bioRxiv - Neuroscience 2024Quote: ... The samples were separated on a 15% Tris-Glycine gel with a 4% stacker using a Tris/Glycine/SDS buffer and transferred overnight onto an Immobilon-FL Transfer Membrane using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked for one hour (0.5% Non-Fat Dry Milk in PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... the proteins were blotted onto methanol-activated polyvinylidene difluoride (PVDF) membranes (0.2 µm) with a Trans-Blot SD Semi-Dry Transfer Cell system (Bio-Rad Laboratories). After blotting ...
-
bioRxiv - Cell Biology 2023Quote: ... proteins incubated with Mal-B were electrotransferred to PVDF membranes using a Trans-blot SD semi-dry transfer cell (Bio-Rad). Biotin incorporation was assessed by incubating blots with HRP-streptavidin (1:1000 v/v ...
-
bioRxiv - Genetics 2024Quote: ... Forty micrograms of whole-cell lysates were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad). The membranes were blocked with 5% nonfat dry milk in Tris-buffered saline with 0.05% Tween 20 (TBST ...
-
bioRxiv - Cell Biology 2024Quote: Proteins were wet-transferred to polyvinylidene difluoride (PVDF) membranes at 100 V for 1 h using a Mini Trans-Blot Cell (Bio-Rad). Membranes were blocked with either 5% milk (CD63 detection ...
-
bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
-
bioRxiv - Microbiology 2023Quote: ... run on a 12% SDS‒PAGE gel and transferred onto a PVDF membrane using a Trans-Blot Turbo electrophoretic transfer cell (Bio-Rad) at 25 V for 30 min ...
-
bioRxiv - Microbiology 2024Quote: ... 1×107 BHK21 cells were electroporated with approximately 2 µg of in vitro transcribed RNA using a GenePulser Xcell electroporator (Bio-Rad) to generate P0 virus stocks.
-
bioRxiv - Systems Biology 2023Quote: ... by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) using a Mini-Protean® Tetra Cell system (BioRad, Neuberg, Germany, 1658005EDU).
-
bioRxiv - Cell Biology 2023Quote: ... and transferred to a nitrocellulose membrane (Maine Manufacturing, LLC) using a Trans-Blot SD Semi-Dry Transfer Cell apparatus (Bio-Rad). Primary antibodies were a rabbit monoclonal anti-HA (Cell Signaling ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The purity and size of the proteins were validated on an SDS-PAGE gel (Mini-PROTEAN TGX and Mini-TETRA cell, Bio-Rad). Purified proteins were stored at 4° C.