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Citations for Bio-Rad :
3101 - 3150 of 3545 citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... 1.0 µg purified plasmid DNA was added to 0.2 ml aliquot of the above-prepared fusobacterial cells in a pre-cooled corvette (0.1 cm electrode gap, Bio-Rad) and left on ice for 10 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The protein concentration of crude cell extracts was measured using the Bradford method with BSA as a standard (Bio-Rad). The reaction mixture contains 8 μg total proteins and 4 μg nitrocefin (Calbiochem ...
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bioRxiv - Microbiology 2022Quote: ... 100-1000 ng) was added to 50 µl of electrocompetent cells and transferred to a 0.2 cm electroporation cuvette (Bio-Rad). Bacteria were electroporated using a Bio-Rad Gene Pulser (25 µF ...
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bioRxiv - Cancer Biology 2023Quote: ... an equal volume of the cell suspension was added to the chamber of the counting slide (3 technical replicates) and counted with the TC20 automated cell counter (BioRad).
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bioRxiv - Microbiology 2023Quote: ... Samples equivalent to 0.1 OD600 nm units of cells were resolved on a 4-20% SDS-polyacrylamide gel (Bio-Rad), and immunoblotted with purified NB13 (16 µg/mL) ...
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bioRxiv - Neuroscience 2023Quote: ... Protein samples were loaded on a 12 % SDS-PAGE gel in the Mini-PROTEAN Tetra Cell (Bio-Rad, 165-8000). Protein was then transferred to a 0.2 µm nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2023Quote: ... A single GFP+ J-Lat 11.1 cell (marking a Tat/Rev msRNA+ cell) was sorted directly into each well of a 96 well white PCR plate (BioRad) containing the RT-LAMP master mix ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were pelleted by centrifugation at 500 rpm for 30 s and resuspended in 4x laemmli-sample buffer (Bio-Rad). Oocyte and embryo samples were boiled at 95 °C for 5 min and separated by SDS-PAGE using Any kD precast Polyacrylamide Gels (Bio-Rad) ...
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bioRxiv - Microbiology 2023Quote: ... pLC10 was introduced into bacterial cells by electroporation using 0.1 cm cuvettes / 1.8 kV pulse following manufacturer recommendations (MicroPulser Electroporator; Bio-Rad Laboratories). Transformants were selected on LB agar plates supplemented with kanamycin 250– 512 µg/mL or apramycin 30-50 µg/mL ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was extracted from sorted cardiac cells or from cardiac tissues (atria or ventricle) using the Aurum total RNA fatty and fibrous tissues kit (Biorad) and following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Protein denaturation before separation was done by treating the cell lysates for 3 min at 95°C in presence of 1x XT Sample Buffer (BioRad) and 1x XT reducing agent (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were lysed and protein reduced by mixing 25μl of sample to 4.75 μl Laemmelli buffer (Bio-Rad #161-0737) and 0.25ul of beta-mercaptoethanol (Bio-Rad #161-0710 ...
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bioRxiv - Cell Biology 2023Quote: cDNA was prepared after extracting total RNA from young adult worms or cell extracts using the iScript cDNA Synthesis Kit (BioRad) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... 2 µg total DNA was added to the cell suspension which was subsequently transferred to an ice-cold 0.1-cm electroporation cuvette (BioRad; #1652089), and electroporated at 0.85 kV/25 µF twice with 5 secs between pulses ...
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bioRxiv - Bioengineering 2023Quote: ... was electroporated (pulsed twice using a square wave of 850 volts for 25 milliseconds) into BHK-21 cells using a Gene Pulser Xcell electroporator (BioRad); transfected cells were cultured in a T175 for 48h at 37 °C ...
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bioRxiv - Developmental Biology 2022Quote: ... was added to cells and incubated for 3 min prior to sorting into 384-well plates (Bio-Rad Laboratories, Inc) prefilled with lysis buffer using the Sony LE-SH800 cell sorter (Sony Biotechnology Inc) ...
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bioRxiv - Developmental Biology 2023Quote: ... was added to cells and incubated for 3 min prior to sorting into 384-well plates (Bio-Rad Laboratories, Inc) prefilled with lysis buffer using the Sony LE-SH800 cell sorter (Sony Biotechnology Inc) ...
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bioRxiv - Cancer Biology 2022Quote: ... FCS and 5 μM EDTA (pH 8.0) (hereafter called FACS buffer) and total cellularity was determined using an TC20 automated cell counter (Bio-Rad). Pellets of 2-5×106 cells were collected for subsequent DNA and protein analysis ...
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bioRxiv - Biophysics 2022Quote: ... 200 ng plasmid expressing Casπ and sgRNA (ccdB-targeting or non-targeting) were electroporated into 50 μL competent cells with 0.2 cm cuvette (BIO-RAD) under 2.5 kV using Eppendorf eporator (Eppendorf) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 µL plasmid was added to 100 µL electrocompetent cells in a chilled Gene Pulser/MicroPulser electroporation cuvette with 0.1 cm gap (Bio-Rad) and incubated on ice for 1 min ...
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bioRxiv - Systems Biology 2023Quote: ... the cells were stained for CD2 expression (using the staining method described above) and analyzed by flow cytometry (BioRad ZE5).
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bioRxiv - Neuroscience 2023Quote: ... and SDS-PAGE was performed on 20 µg of cell lysate per sample on Tris-glycine-SDS polyacrylamide gels (4561095, Biorad) then transferred to PVDF (1620177 ...
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bioRxiv - Microbiology 2023Quote: ... 390 μl of cells were mixed with 10 μg of in vitro transcribed RNA and added to a 2 mm electroporation cuvette (BioRad). BHK-21 cells were electroporated with 1 pulse at 1.2 kV ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were run on 12 % SDS-PAGE gels and transferred to 0.45 µm pore nitrocellulose membranes (Bio-Rad 1620251) using a wet transfer system (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA from the barcoded cells for each sample were subsequently reverse-transcribed in a C1000 Touch Thermal cycler (Bio-Rad) and all subsequent steps to generate single-cell libraries were performed according to the manufacturer’s protocol with no modifications (13 cycles were used for cDNA amplification) ...
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bioRxiv - Cell Biology 2023Quote: ... 150ul of the suspension was filtered through a 40μM cell strainer at 40G for 5 minutes to remove clumped cells and subsequently used for analysis on the ZE5 flow cytometer (Biorad). Analysis of gene expression was analyzed using the Easyflow MATLAB script (https://antebilab.github.io/easyflow/) ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA was separated in 1.45 L of 1x TBE at 2.0 V/cm for 20–22 hours with buffer circulation in a Sub-cell GT electrophoresis system (Bio-Rad). DNA was vacuum-transferred to Hybond-XL (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... 0.5 μl of this cell suspension was then placed on a 1.5% (w/v) low melting agarose (LMA; Bio-Rad) TGY2X pad prepared on a cover glass using a gene frame (Thermo Scientific) ...
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bioRxiv - Immunology 2023Quote: ... and 5 h later cells were stained with mAbs against CD4-PerCP-Cy5.5 (as in Table S2) and CD8β-FITC (clone PPT23, Bio-Rad). The cells were fixed and permeabilized with BD Cytofix/Cytoperm (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from vehicle (DMSO)-treated and 1 μM CHIR-treated cells was extracted using an Aurum Total RNA Kit (BioRad) and stored at -80°C ...
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bioRxiv - Molecular Biology 2023Quote: Preparation of the electrocompetent DH5α cells and electroporation were done following the instruction manual of the micropulser electroporator (Bio-rad). After electroporation ...
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bioRxiv - Bioengineering 2023Quote: ... The eluate was quantified from 20ul aliquots that were mixed with trypan blue and counted twice using the TC20 automated cell counter (BioRad). Cells were pelleted and resuspended in 1 mL of Cytofix/Cytoperm solution (Becton Dickinson ...
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bioRxiv - Cell Biology 2023Quote: ... For each sample 20 μg of K562 cell extracts were loaded on 6-18% hand-casted acrylamide SDS-PAGE gradient gel (40% Acrylamide/bis-Acrylamide solution, BioRad). After separation by electrophoretic run ...
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bioRxiv - Cancer Biology 2023Quote: ... Nuclei were then isolated on an iodixanol gradient and counted after addition of trypan blue using a TC20 automated cell counter (Biorad). 5.000-50.000 counted nuclei were transferred to a new tube and were centrifuged at 500g before proceeding with the transposition reaction ...
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bioRxiv - Developmental Biology 2024Quote: ... the absolute number of NEUROG2-mCherry KI gene copies per cell was quantified and normalized to RPP30 (Bio-Rad, 10031243). 20 ng of genomic DNA was used in 20 µl PCR reaction containing 900 nM of the forward and reverse NEUROG2-mCherry KI and RPP30 primers ...
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bioRxiv - Cell Biology 2024Quote: ... The cell lysates were fractionated by SDS-PAGE on a 10% gel or a 4-15% gradient gel (Bio-rad) and the separated proteins were transferred to a polyvinylidene difluoride membrane (Merck) ...
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bioRxiv - Plant Biology 2024Quote: ... Proteins were transferred from gels to the PVDF membrane using the Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad). Membranes were incubated in 5% nonfat dried milk in 1X TBST (Tris-buffered saline ...
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bioRxiv - Neuroscience 2024Quote: ... The gel was transferred to a 0.22 μm nitrocellulose membrane (AmershamTM Protran, G10600080) through Mini Trans-Blot Electrophoretic Transfer Cell (BioRad, 1703930) for 60 min at 100 V ...
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bioRxiv - Cell Biology 2024Quote: ... and approximately 1 μg of plasmid DNA was electroporated into the cells by pulsing twice at 1000 V with the Xcell gene pulser (Biorad). The cells were incubated with subsequent addition of healing solution (2 mM CaCl2 ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto a 0.45 µm PVDF membrane (Carl Roth, T830.1) by using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad). The membrane was blocked with 5 % BSA (w/v ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of the cell-oligonucleotide mixture was transferred to a pre-chilled 1 mm electroporation cuvette (BioRad catalog #1652083) and transformed with 1.8 kV ...
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bioRxiv - Biochemistry 2024Quote: ... Cell lysates (30 μg) were separated on precast 4-15% or 7.5% SDS-polyacrylamide Criterion gels (Bio-Rad, Hercules, CA) and transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2024Quote: ... proteins were transferred onto a nitrocellulose membrane using the Mini Trans-Blot® Cell and Criterion™ Blotter (Bio-Rad). Proteins on the membrane were stained with Ponceau S buffer ...
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bioRxiv - Synthetic Biology 2024Quote: ... Protein purity and size were validated on an SDS-PAGE gel (Bio-Rad Mini-PROTEAN TGX and Mini-TETRA cell). Purified proteins were stored at –80°C.
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bioRxiv - Synthetic Biology 2024Quote: ... 10 million induced cells from each track were washed and labelled for HA and c-Myc detection as described above and sorted on an S3e cell sorter (BioRad) to isolate cells that show HA detection ...
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bioRxiv - Immunology 2024Quote: ... Stained cells were resuspended in FACS buffer and acquired with a 5-laser Bio-Rad ZE5 flow cytometer (Bio-Rad). FACS data were analyzed using FlowJo version 10.9 software (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: T cells were washed once with ice-cold PBS and immediately lysed with 4x laemmli buffer (Bio-Rad, cat. 1610747). Cell lysates were separated by SDS-PAGE and blotted with anti-Kcnn4 antibody (cat ...
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bioRxiv - Cancer Biology 2021Quote: Protein samples from LNCaP cells used to measure luciferase activity (described in Fig. 4A) were loaded into 4% to 15% SDS-PAGE (Bio-Rad) and subjected to electrophoretic analysis and subsequent blotting ...
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bioRxiv - Cancer Biology 2021Quote: The prepared whole cell lysates were used to conduct gel electrophoresis followed by transfer to a polyvinylidene fluoride membrane (Bio-Rad). The membrane was blocked in 5% milk solution in Tween-PBS (PBS with 0.1% Tween 20 ...
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bioRxiv - Biochemistry 2021Quote: ... Cell sorting was performed on the Bio-Rad Sorter S3e instrument with the Software ProSort 1.5 (Bio-Rad, Hercules, California, USA).