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Citations for Bio-Rad :
2951 - 3000 of 3002 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Protein extracts were obtained from MAYV- or UNAV-infected HMC3 or HBEC-5i cells in Laemmli buffer with 10% dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were fractionated in SDS-PAGE ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... DNA was diluted to 10 ng/μl and 2 μl were used in each RT-qPCR reaction with 10 μl of iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA) and 400 nM of forward and reverse primers ...
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bioRxiv - Immunology 2023Quote: ... Leaves were kept in the dark for 10 min before luminescence was detected with a ChemiDoc™ XRS+ imagine system (Bio-Rad, Feldkirchen, Germany). Luminscence intensities/mm2 infiltrated leaf area of different combinations were evaluated using the Image Lab software (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 3.6 μL of ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The expression of the housekeeping gene GADPH (XM393605 ...
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bioRxiv - Genetics 2023Quote: ... washed twice with 20 ml wash buffer 1 (= lysis buffer + 20 mM imidazole) and then at room temperature packed into a 10 ml Polyprep Chromatography Column (Bio-Rad Laboratories, Feldkirchen, Germany). The protein was eluted from the column with 5 ml elution buffer 1 (= lysis buffer + 500 mM imidazole) ...
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bioRxiv - Biochemistry 2023Quote: ... The elutions were then concentrated on an Amicon spin column (10 kDa cutoff) and quantified by the DC assay (Bio-Rad, Hercules, CA, USA). The eluted protein was then diluted in PBS (pH 7.4) ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: Cell or tissue lysates (10–30 μg protein) or immunoprecipitated samples were resolved by SDS–PAGE and transferred to nitrocellulose membranes (Bio-Rad Laboratories, Hercules, CA). The membranes were incubated with antibodies against leptin (Cat# ...
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bioRxiv - Evolutionary Biology 2023Quote: ... real-time PCR was performed in a reaction volume of 20 μL comprising 10 μL Mastermix with SYBR green (Bio-Rad, Hercules, CA, USA), 0.6 μL each primer (1.2 μM) ...
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bioRxiv - Microbiology 2024Quote: ... 10*106 virions of IMP-1088 or DMSO released purified viruses were lysed in 4x Laemmli protein sample buffer (BIO-RAD, Cat. no. 1610747). Samples were electrophoresed on 4-20% precast polyacrylamide gel (BIO-RAD ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of protein (20 μg/lane) were resolved in 10% Bis-Tris Criterion™ XT Precast Gels and XT MOPS running buffer (BioRad, Cat# 1610799, USA) at 120 volts for 1.5 h using Criterion™ vertical electrophoresis cell (BioRad ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time quantitative polymerase chain reactions (RT-qPCR) were performed in 10 μl reactions containing Power SYBER Green PCR master mix (Bio-Rad; Cat #: 172-5121), 5 ng of cDNA ...
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bioRxiv - Molecular Biology 2024Quote: ... Protein samples (10 µg of protein per well) were separated on SDS-polyacrylamide gels (CriterionTM TGXTM Precast Gels, 4-15%; Bio-Rad, Hercules, CA, USA) using a Laemmli buffer system and then semidry transferred to nitrocellulose membranes by a Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... The collected protein samples were electrophoresed on 10% SDS-polyacrylamide gel (SDS-PAGE) and transferred to the polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). After blocking with 5% bovine serum albumin (BSA ...
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bioRxiv - Microbiology 2024Quote: ... A 40 µg of protein of CFS samples and 10 ng of purified rGdhA were resolved in a 4-20% SDS-PAGE (Bio-Rad, Hercules, CA; # 4561094) and immunoblotted with affinity purified anti-rabbit rGdhA antibody (0.3 µg/mL ...
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bioRxiv - Microbiology 2024Quote: ... The proteins were then separated by electrophoresis on a 10% SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Cat. No. 1620177). The membrane was blocked with 5% bovine serum albumin (B.S.A. ...
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bioRxiv - Microbiology 2024Quote: ... resolved on the 10% SDS PAGE and transferred to nitrocellulose membrane (Trans-Blot Turbo Midi Nitrocellulose Transfer Pack, Bio-Rad, 0.2 µm pore size) with the use of a Trans-Blot Turbo Transfer Starter System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: 20 µg of proteins were separated on a 10% polyacrylamide gel and transferred on nitrocellulose membranes (Amersham™ Protran®; #106000004) using Trans-Blot® Turbo™ (BioRad). Blocking was performed through a 1h incubation in 5% low fat milk in TBST ...
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bioRxiv - Neuroscience 2024Quote: ... at 95°C for 10 mins before being loaded into 4-20% Mini-PROTEAN® TGXTM Precast Protein Gels (15 wells, 15uL; Bio-Rad, CA, USA) and subject to electrophoresis on a Bio-Rad Mini-PROTEAN® Tetra Cell system (180 V ...
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bioRxiv - Cell Biology 2020Quote: ... SDS-solubilized proteins (0.2-0.3 OD600 cell equivalents) were resolved by 10% SDS-PAGE prior to transfer to nitrocellulose membranes using the Trans-Blot Turbo™ system (Bio-Rad Laboratories, Hercules, CA) and blocked with Western Blocking Reagent (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Each 20 μL qPCR reaction was prepared in UV-treated UltraPure nuclease-free water containing 10 μL of EvaGreen® Supermix (Bio-Rad Laboratories, Hercules, CA), 0.5 μL of each primer (forward and reverse ...
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bioRxiv - Neuroscience 2019Quote: ... Reactions were carried out in triplicate on 96-well plates with 10 µL reactions per well using a CFX96 Touch™ Real-Time PCR Detection System from Bio-Rad (Hercules, CA, USA). Cycling conditions for qPCR reactions were as follows ...
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bioRxiv - Microbiology 2020Quote: ... Amount 40 µg of total protein was run in 10% polyacrylamide gel at the same electrophoresis condition and then the gel was stained in coomassie blue solution (Bio-Rad Laboratories, Berkeley, USA; #1610786).
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bioRxiv - Genetics 2022Quote: ... Real time (RT)-qPCR was carried out in a Bio-Rad® CFX-96™ thermocycler in 10 μl final volume reaction using SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad) and 50 pmol of each forward and reverse primer (Table S1) ...
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bioRxiv - Neuroscience 2021Quote: ... and half of each biotinylated fraction were separated into 10% SDS-PAGE gels electrophoresis and transferred to nitrocellulose membranes (Bio-Rad Laboratory, Hercules, CA, USA). Subsequently ...
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bioRxiv - Microbiology 2020Quote: ... pelleted by centrifugation and resuspended in RPMI medium containing 10% foetal bovine serum where cell count and viability was estimated (BioRad, TC20™ Automated Cell Counter) before plating for experiments.
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of proteins from lysates were separated by SDS-PAGE using Mini-PROTEAN® TGX™ 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were transferred to new tubes containing 87% glycerol (final concentration of 10%) and concentrations were adjusted using Bio-Rad protein assay (Bio-Rad, Hercules, California, United States).
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bioRxiv - Biochemistry 2023Quote: ... The resin was recovered and washed twice with 10 column volumes of S100 Lysis Buffer on a gravity flow column (Bio-Rad Laboratories Inc., Hercules, CA). The S100 extract was eluted from DEAE resin with 5 ml of S100 Elution Buffer (250 mM KHPO4 (pH 6.5),1x Mini protease inhibitor cocktail tablet (Roche ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was washed three times for 10 mins with TTBS and once for 10 mins with TBS before developing it with the Clarity Western ECL Substrate (Bio-Rad Laboratories, cat. #170-5061) and visualizing it using the Bio-Rad ChemiDoc MP Imaging system ...
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bioRxiv - Cell Biology 2023Quote: ... The membrane was washed again with 1x PBS-T (0.075 %) 3 times 10 minutes each and incubated with 1:1 ECL chemiluminescence solution (Clarity Western ECL, #170-5061, Bio-Rad Laboratories, Hercules, CA, USA). Signal was detected using an Amersham AI600 imager (Supplementary Table 5).
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bioRxiv - Cancer Biology 2023Quote: ... 20 μg of total proteins were resolved on 10% polyacrylamide gels (except for 6% gels used for PERK detection) and blotted onto PVDF membranes (#1620177, Bio-Rad Laboratories, Hercules, CA, USA). The membranes were blocked with 5% not-fat dry milk or bovine serum albumin (#A7906 ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 μg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Molecular Biology 2023Quote: ... The same amount of cell lysates or immune precipitates was resolved by 10% SDS-PAGE and transferred to the PVDF membrane (Bio-Rad Laboratories, Hercules, CA, USA) by electrophoresis ...
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bioRxiv - Biochemistry 2023Quote: ... BioRad Bradford Assay was carried out and 10 µg of each sample was applied in SDS-PAGE gels and then transferred to a PVDF membrane (Bio-Rad, 0.45 µm pore size) by electro-transfer (30 V constant overnight ...
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bioRxiv - Neuroscience 2023Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad, cat. no. 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Bioengineering 2023Quote: ... The qPCR reactions were performed in a total volume of 20 µL with 10 µL of iTaq™ Universal SYBR® Green Supermix (Bio-Rad Laboratories Inc.), 0.7 µL of the forward primer (350 nM) ...
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bioRxiv - Neuroscience 2024Quote: ... was boiled for 10 min at 97°C and loaded into 8% SDS-PAGE gels with Precision Plus Protein Standard (Bio-Rad, 161-0374/161-0375). Separated proteins were transferred to a nitrocellulose membrane (0.2 μm ...
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bioRxiv - Molecular Biology 2024Quote: ... were resolved on a 4-15% or 10% acrylamide gel and transferred onto a PVDF membrane (Invitrogen iBlot 2 or Bio-Rad Trans-Blot Turbo system). Membranes were blocked with 5% milk (standard protein detection ...
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bioRxiv - Molecular Biology 2020Quote: ... The PCR amplification conditions were initial denaturation at 95°C for 10 min followed by 40 cycles of 95°C for 10 s and annealing at 58-65 °C for 30 s (Bio-Rad CFX Connect Real-Time PCR) followed by melt peak analysis ...
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bioRxiv - Microbiology 2020Quote: ... The reaction mixture had a volume of 20◻μL and comprised of 10◻μL of ddPCR supermix for probes (BioRad Laboratories, Hercules, CA, USA), 1◻μL of cDNA template ...
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bioRxiv - Microbiology 2020Quote: ... 8.4 μL of PCR Probe water and 10 μL of the 2x commercial reaction mixture (SYBR® Green Master Mix, Bio-Rad. A LightCycler96® (Roche) device was used and PCR conditions applied were ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min before loading into a 4 – 20% Criterion TGX precast Midi protein gel (Bio-Rad, Hercules, CA, USA; Cat# 5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Cancer Biology 2019Quote: ... were separated on 10% or 4-20% gradient SDS–PAGE gels and wet-transferred to PVDF using the mini-PROTEAN® TGX™ system (Bio-Rad, Hercules, CA, USA). Membranes were pre-blocked with 5% low-fat dry milk in TBS-T and incubated with a rabbit polyclonal anti-KHSRP antibody (Sigma-Aldrich Cat# HPA034739 ...
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bioRxiv - Microbiology 2022Quote: ... Amplification of targeted genes was carried out in duplicate 20 μL reactions containing 10 μL of SsoFast™ EvaGreen® Supermix (Bio-Rad Laboratories, Hercules, CA), 2 μL of DNA template ...
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bioRxiv - Plant Biology 2022Quote: ... 20 pmol of each primer in a total reaction volume of 10 μL on a C1000™ Thermal Cycler with a CFX384™ Touch Realm-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) with the following conditions ...
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bioRxiv - Microbiology 2024Quote: ... Protein was transferred to a PVDF membrane (hydrated in methanol and prewet in 1X Transfer Buffer) between blotting paper stacks in the Trans-blot Turbo (Bio-Rad; 1.6 A for 10 min). The membrane was cut at the 70 kDa ladder marker ...
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bioRxiv - Microbiology 2021Quote: ... 10 or 1 µl of total lysate and 10 or 1 µg of OMVs were separated on Criterion™ TGX Stain-Free™ any kD™ gel (Bio-Rad, Hercules, California, USA) for Coomassie staining or Western Blot ...
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bioRxiv - Immunology 2021Quote: ... with 0.5 μl of b-mercaptoethanol per well and heated at 70°C for 10 minutes before separation on a polyacrylamide gel (Bio-Rad Mini-PROTEAN TGX Gel 4-15%) and transferred to a PVDF membrane ...
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bioRxiv - Cell Biology 2021Quote: ... Resulting protein extracts were subjected to 10 % (w/v) SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA, 0.2 μm pore size). Upon incubation with 5 % (w/v ...