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Citations for Bio-Rad :
2901 - 2950 of 3002 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... Reagents on plates were gently mixed by rotational agitation for 10 min before measuring the absorbance at 570 nm using a microplate reader (Bio-Rad, 680 XR).
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bioRxiv - Biophysics 2021Quote: ... and 3.6 μL ddH2O in a 10 μL total reaction volume using Bio-Rad CFX Connect™ Real-Time PCR System (Bio-Rad, CA, USA). The 2-ΔΔCt method was used for the quantitative analysis ...
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bioRxiv - Molecular Biology 2021Quote: ... 3 uL of a 1:20 dilution of the cDNA was used as input into each 10 uL qPCR reaction using the SsoAdvanced™ Universal SYBR® Green Supermix (BioRad; Cat #: 1725271). mex-6 ...
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bioRxiv - Cancer Biology 2019Quote: ... Nuclear extracts containing the total of 40 µg protein were separated on a 10% Tris-SDS PAGE gel (Bio-Rad Mini-PROTEAN® TGX). Gels were transferred on nitrocellulose membranes using Bio-Rad TransBlot system ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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bioRxiv - Genetics 2021Quote: ... and purity assessed by 15% SDS-PAGE using Bio Rad Precision Plus Protein All Blue Standards (10-250 kDa) as molecular weight ladder (Bio-Rad; Hercules, CA, USA).
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bioRxiv - Microbiology 2021Quote: ... For 16S rRNA gene analysis the 20 μl dPCR pre-droplet mix consisted of 10 μl dPCR Supermix for Probes (Bio-Rad Laboratories, Munich, Germany), 1 µl 20x 16S SNP Primer mix (final concentrations 900 nM) ...
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bioRxiv - Plant Biology 2020Quote: ... 0.02 µg of cDNA was used in a 20 µl reaction containing 10 µl of SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad, US), 6 µM of forward and reverse primers and 3 µl of RNAse-free water ...
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bioRxiv - Microbiology 2020Quote: ... 10 μg of transcribed RNA were electroporated into 8×106 Vero cells using the Gene Pulser Xcell™ Electroporation Systems (Bio-rad, California, USA) under as described previously41 ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed 3X in PBST (10 min) and incubated for 1 h with horseradish peroxidase conjugated anti-rabbit (BioRad 170-6515, lot #: 64033820) or anti-mouse (GE Healthcare UK Limited NA931V ...
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bioRxiv - Microbiology 2020Quote: ... Cell suspensions were clarified by centrifugation (20 000 x g, 10 minutes, 4°C) and total soluble protein quantified58 using a kit (Bio-Rad Protein Assay Kit) and BSA as a standard.
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bioRxiv - Cancer Biology 2021Quote: Separation of 20 μg or 50 μg protein from cell lysate was performed on a 10% Mini-PROTEAN® TGX™ gel (BioRad, Portland, ME) and subsequently transferred onto Immunoblot PVDF 0.45μm membranes (BioRad ...
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bioRxiv - Microbiology 2022Quote: ... protein extracts were obtained from mock-or MAYV-infected HeLa cells or HDFs that were treated or untreated with PIM kinase inhibitors in Laemmli buffer with 10% Dithiothreitol (Bio-Rad, Hercules, CA, USA). Proteins were separated in SDS-PAGE ...
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bioRxiv - Biophysics 2022Quote: ... The conformational homogeneity of RNA sample was checked with an ENrich™ SEC 650 10 x 300 Column (Bio-Rad; column volume: 24 mL). RNA (20 μM ...
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bioRxiv - Genetics 2022Quote: ... the membranes were washed 3 times for 10 min each with TBST buffer at 24 °C and visualized using enhanced chemiluminescence reagents (Bio-Rad Laboratories, CA, USA). The images were captured using Image Quant LAS 4000 (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2021Quote: ... qPCRs were carried out using 10 ng of cDNA and 0.4 mM of primers and 2X SsoFastTM EvaGreen® Supermix (Bio-Rad, Hercules, CA, USA) in a total volume of 10 μL ...
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bioRxiv - Immunology 2020Quote: A Pre-packed HiLoad™ 16/60 Superdex™ 200 pg (Cytiva, catalog #28989335) on the NGC™ Quest 10 Plus Chromatography System by Bio-Rad was calibrated at room temperature using the HMW Gel Filtration Calibration Kit (Cytiva ...
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bioRxiv - Neuroscience 2021Quote: ... to a final concentration of 10 mM and shielded from light for 30 minutes.We performed an automated detergent compatible protein assay (DCA) (Bio-Rad, Hercules, CA, Cat. #5000111), normalization (to 1 μg/μL ...
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bioRxiv - Bioengineering 2021Quote: ... incubated for 5 min at 95°C and used as template in the qPCR reaction in a final 10−4 dilution with the iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, Hercules, CA). The qPCR was run in a StepOnePlus Real-Time PCR System (Applied Biosystems ...
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bioRxiv - Bioengineering 2021Quote: All analyses were performed using auto-sampling a 10 microliter to injected in an Aminex HPX-87H column (Bio-Rad Laboratories, Hercules, CA, USA), analysed in a refractive index detector (RID-10A ...
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bioRxiv - Biophysics 2021Quote: ... We electroporated the cells with two pulses (exponential decay pulse mode, 1500 V, 24 µF) separated by 10 s (Gene Pulser Xcell Electroporation System, Bio-Rad Laboratories, Inc., CA). We added 12 mL of growth media supplemented with 15 µg/mL G418 and 50 µg/mL hygromycin and put 0.5 mL of the electroporated cells per well into a 24-well plate ...
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bioRxiv - Cancer Biology 2022Quote: ... Cell concentration was calculated by adding 10 µL of cell suspension to chip for Biorad TC20 Automated Cell Counter (Bio-Rad Laboratories, Richmond, CA).
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bioRxiv - Systems Biology 2019Quote: ... cDNA was diluted 2X before use and qRT-PCR reactions were prepared in a 10 μl volume using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) and 400 nM specific primers targeting the gene of interest (SLC10A2-F [5’-TGGGTTTCTTCCTGGCTAGACT-3’] ...
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bioRxiv - Developmental Biology 2020Quote: Three technical replicates of 10 ul qPCR reactions per sample were prepared using Bio-Rad SsoAdvanced universal SYBR green master mix (Bio-Rad, Hercules, CA 1725271) and were run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1855195) ...
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bioRxiv - Physiology 2019Quote: ... Defined amounts of mRNAs at 10-fold dilutions were amplified via real-time PCR using iTaq™ Universal SYBR® Green One-Step kit (Bio-Rad, Italy) using the following RT-PCR conditions ...
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bioRxiv - Immunology 2019Quote: ... a real-time PCR reaction mixture was prepared containing 10 µL of SsoAdvanced™ SYBR® Green Supermix (Bio-Rad, cat. no. 172-5265), 1 µL each of 5 µM gene-specific forward and reverse primer pair ...
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bioRxiv - Cancer Biology 2019Quote: ... 50ug protein was separated on 10% ECL gels (GE, Pittsburgh, PA) and transferred to nitrocellulose using the Trans-Blot® System (Bio-Rad, Hercules, CA). Antibodies are listed in Supplemental Table S10.
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bioRxiv - Cell Biology 2019Quote: ... Separation in the second dimension was accomplished using an 10% SDS-PAGE in a Protean II xi 2-D Cell (Bio-Rad, Hercules, CA, USA) at 50 mA until the bromophenol blue reached the bottom of the gel ...
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bioRxiv - Genetics 2020Quote: Cell viability was measured after collecting conditioned medium from BLRR cells by adding 1/10 volume of alamarBlue reagent (Bio-Rad, Hercules, California, USA) to samples followed by incubation at 37°C with 5% CO2 for 1 h ...
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bioRxiv - Synthetic Biology 2021Quote: ... diluted 1:200 in 10 mM Tris-HCl pH 7.5 and 9 μL loaded (along with 1 μL 1 M DTT and 10 μL 2X Laemmli Buffer) into an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-rad). For Western blot ...
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bioRxiv - Neuroscience 2021Quote: ... total protein was extracted and 15-20µg from each sample was separated on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Biorad, Hercules, CA., USA) adjacent to the Li-COR Chameleon ladder (Li-Cor ...
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bioRxiv - Immunology 2021Quote: ... IL-6, TNF-α, IL-8, IP-10, MCP-1, MIP-1α, MIP-1β, IL-1RA, G-CSF and Eotaxin; Bio-Rad Laboratories Inc.) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... a real-time PCR reaction mixture was prepared containing 10 µL of SsoAdvanced™ SYBR® Green Supermix (Bio-Rad. Cat. No. 172-5265), 1 µL each of 5 µM gene-specific forward and reverse primer pair ...
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bioRxiv - Plant Biology 2021Quote: ... qPCRs were carried out using 10 ng of cDNA and 0.4 mM of primers and 2X SsoFastTM EvaGreen® Supermix (Bio-Rad, Hercules, CA, USA) in a total volume of 10 μL ...
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bioRxiv - Biochemistry 2021Quote: ... Premade 4-20% TGX gels with either the 10-well 50 μL or 15-well 15 μL formats (Bio-Rad cat. # 4561094 and 4561096) were used in all instances ...
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bioRxiv - Synthetic Biology 2021Quote: ... A 250 μL suspension of cells and 500 ng of double-digested (XbaI and KpnI) vector plasmid were incubated for 5–10 min on ice in a 4-mm Gene Pulser®/MicroPulser™ cuvette (BioRad, Hercules, CA). GenePulser XCellTM (BioRad ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Neuroscience 2021Quote: Each sample was run in three technical replicates per gene in a 10 uL qPCR reaction using Bio-Rad SsoAdvanced universal SYBR green master mix (Bio-Rad, Hercules, CA 1725271). Samples were run on a CFX96 Touch Real-Time PCR Detection System (Bio-Rad 1855195) ...
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bioRxiv - Immunology 2021Quote: ... supernatants or virions were separated on 10% SDS polyacrylamide gel under reducing conditions and transferred to a nitrocellulose membrane with a Trans-Blot Turbo System (Bio-Rad, Hercules, CA, USA). Filters were saturated for 1 h with 5% nonfat dry milk in TBST (TBS with 0.1% Tween 20 ...
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bioRxiv - Genomics 2021Quote: When cDNA was used as a template the 20 μl ddPCR mix consisted of 10 μl ddPCR Supermix for Probes (Bio-Rad Laboratories, Munich, Germany), 1 μl 20x UBC primer and probe mix (Table 3 ...
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bioRxiv - Biochemistry 2022Quote: Protein overexpression and purification were analysed via 10% SDS-PAGE and imaged via ChemiDoc™ MP Imaging System (Bio-Rad; Fig. S1 and S4). Immunoblotting was performed to confirm purification results with primary anti-6xHis polyclonal mouse antibody (Beyotime® ...
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bioRxiv - Neuroscience 2022Quote: ... was prepared by mixing the following reagents in a total volume of 20 µl: 2x QX200 ddPCR EvaGreen supermix (Biorad, catalog #1864036, 10 µl), forward and reverse primers (10 µM ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... An equal amount of total protein was resolved by a 10% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). Membranes were blocked and incubated with either anti Phospho-(Ser/Thr ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to PVDF transfer membrane (IPVH00010 Immobilon®-P; MilliporeSigmaTM, Burlington, USA) overnight at 10 mA in a cold room (Mini trans-blot cell; Bio-Rad, Hercules, USA). The membrane was blocked for non-specific sites and incubated with anti-HvHsfA6a in 1:2000 dilutions overnight at 4 °C ...
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bioRxiv - Genetics 2024Quote: ... The cDNAs were individually diluted 10-fold for use as templates for subsequent qPCR with iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories, USA, 1725124). Mouse Gapdh was used as an internal control ...
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bioRxiv - Cell Biology 2024Quote: ... The resolved proteins were transferred on to a 0.22 µ nitrocellulose membrane (#10600004, Cytiva, US) for 60 min at 10 V using the Trans-blot semi-dry transfer apparatus (Bio-Rad Laboratories Inc., US). The blots were blocked with 4 % skimmed milk (#2325 ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were separated using 10% SDS page and blotted on a nitrocellulose membrane using the Trans-Blot Turbo Transfer System (Bio-Rad Laboratories, Inc., Austria). The membrane was probed with the anti pY-4G10 (mouse ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed 3x in PBST (10 min) and incubated for 1 hr with horseradish peroxidase conjugated anti-rabbit (BioRad 170-6515, lot #: 64033820) secondary antibodies (1:10000 ...
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Paralog editing tunes rice stomatal density to maintain photosynthesis and improve drought tolerancebioRxiv - Plant Biology 2022Quote: ... coated with 5 mg of plasmid DNA for editing rice STOMAGEN were divided equally among 10 macro-carriers and used for bombardment with a Bio-Rad PDS-1000 He biolistic device (Bio-Rad, Hercules, CA, USA) at 650 psi ...
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bioRxiv - Neuroscience 2023Quote: ... the incubated membranes were washed again three times with 1x TBST for 10 minutes each at RT and visualised with Clarity Western ECL Substrate (BIO-RAD, Cat. #170-5060).