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Citations for Bio-Rad :
2751 - 2800 of 3002 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 10 μg of total cell lysates proteins were separated by electrophoresis on 10% SDS polyacrylamide gels (previously prepared following the Bio-Rad protocol) and transferred to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Genetics 2021Quote: ... Equal amounts of proteins (40 μg) were loaded onto 10% Tris-glycine gel (CriterionTM XT precast gel) using XT Tricine Running Buffer (Bio-Rad, USA). After electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... and 10-14h after egg laying and reverse transcribed using oligo-dT primers with a One-Step RT kit (Bio-Rad 1725140). The coding sequence for each gene encoding a gene of interest was amplified from a cDNA template using Phusion High Fidelity DNA polymerase (Thermo Fisher F-530XL) ...
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bioRxiv - Neuroscience 2021Quote: ... was incubated at 65°C for 10 min and at 85°C for 1 min into a T100™ thermocycler (Bio-Rad). The heated lysate was subsequently used in cDNA synthesis with SuperScript™ III Reverse Transcriptase kit in a total volume of 20 μl following manufacturer protocol (Invitrogen™) ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were centrifuged at 20,000 x g for 10 min at 4°C and protein concentration in the supernatant was determined by Bradford assay (Bio-Rad, 5000202) and normalized across samples ...
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bioRxiv - Immunology 2021Quote: ... cDNA synthesis was performed using 3 ng (neutrophils) and 10 ng (monocytes) of total RNA and iScript™ Reverse Transcription Supermix (Bio-Rad). Relative quantitation of mRNA expression levels was determined with CFX96 Touch™ Real Time PCR using SsoAdvanced™ Universal SYBR Green Supermix and PrimePCR™ Primers (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... and purified from remaining salts using Dowex 50 W X 8 50-100 mesh (Bio-Rad, 10 x 1 cm, H+ form) equilibrated with water ...
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bioRxiv - Evolutionary Biology 2021Quote: ... 0.5 µL of each forward and reverse primer (10 µM) and 5 µL of SsoADV Universal SYBR® Green Supermix (Bio-Rad) were used ...
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bioRxiv - Immunology 2020Quote: ... Conjugate DNA was measured by qPCR amplification from 1 μL of lysate in a 10 μL reaction containing 5 μL of iTaq Universal SYBR Green Supermix (Bio-Rad, 1725125) and 5 pmol of each primer (Supplementary Table 1) ...
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bioRxiv - Immunology 2021Quote: ... development buffer was added to each well and the plates were read every 10 minutes using a Model 550 Microplate Reader (Bio-Rad, UK) until the positive control reached a predetermined OD405 that was consistent across plates ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by polyacrylamide gel electrophoresis (PAGE) on a 10% mini-PROTEAN® TGX™ precast gel (Bio-Rad, CA, USA) at 140 V and 300 mA for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting pellets were resuspended in homogenization buffer and centrifuged again at 9000 g for 10 min at 4°C and protein concentration was measured using the Quick Start Bradford Protein Assay Kit 1 (Bio-Rad 5000201). Mitochondrial respiration was measured in Mitochondrial Assay Solution (MAS ...
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bioRxiv - Cancer Biology 2022Quote: ... prestained protein ladder were resolved on 10% acrylamide gels by SDS-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose membrane (BioRad Cat. # 1620112) at 100 volts for 90 min.
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bioRxiv - Bioengineering 2022Quote: ... Samples (10-15ug protein/lane) were run by electrophoresis on pre-cast SDS-Page Stain Free Gels (Bio-Rad, Hercules, CA, USA). Separated proteins were transferred to low fluorescence PVDF membranes using the TransBlot Turbo (Bio-Rad ...
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bioRxiv - Developmental Biology 2022Quote: ... and 20 ng of cDNA (based on the original RNA concentration) were used for qPCR analysis in 10 ul reactions with SYBR Green (Bio-Rad, 1725124). Expression was calculated relative to the geometric mean of the two housekeeping genes.
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bioRxiv - Genetics 2022Quote: ... resuspended to 10 million cells/ml and sorted on a two-laser (466 nm and 561 nm) cell sorter (Bio-Rad S3) as previously described (55) ...
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bioRxiv - Synthetic Biology 2022Quote: ... qPCR was performed to determine the copy numbers of the EcN fimA gene in 10 ng of fecal genomic DNA using iTaq Universal SYBR Green Supermix (Bio-Rad, USA). Standard curves were constructed by quantitatively testing 108 ...
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bioRxiv - Microbiology 2022Quote: ... the proteins were separated on a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and then transferred onto a polyvinylidene difluoride (PVDF) membrane (Bio-Rad, USA). The blots were blocked in 5% BSA in Tris-buffered saline with Tween 20 (TBST ...
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bioRxiv - Neuroscience 2022Quote: ... The diluted cDNA (1:10) was used as a template for real-time PCR (Bio-Rad CFX 96™ Real-Time system) with an SYBR® Green Supermix mixture following the manufacturer’s protocol (Bio-Rad) ...
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bioRxiv - Plant Biology 2023Quote: Equal amounts (10 µg) of nuclear proteins from different samples were separated using SDS– PAGE and electrotransferred to a nitrocellulose membrane (BioRad, 162-0115) for 1.5 h at constant 400 mA at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... and 0.01% Silwet L-77 (Lehle Seeds Inc) solution followed by incubation in the dark for 10 min and imaging using a ChemiDoc™ Touch System (Bio-Rad). Alternatively ...
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bioRxiv - Neuroscience 2022Quote: ... while brain cytokine analyses for Studies C and D were conducted using a custom 10-plex Bioplex (Bio-rad Laboratories, Hercules, CA) as previously described (Finnell ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (∼15 μg) were loaded and separated on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, Hercules, CA), and transferred to polyvinilidene difluoride membranes (PVDF ...
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bioRxiv - Bioengineering 2022Quote: ... were separated on a 4-10% SDS-polyacrylamide gel at 120 V for two hours using a PowerPac Basic setup (BioRad Laboratories Inc.). The proteins were transferred onto a 0.45 µm nitrocellulose membrane at 75 V for 90 min using a transfer assembly (BioRad Laboratories Inc.) ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein measurements were performed using a BCA kit (Pierce) and 150 µg was loaded on a 10-20 % tris/tricine SDS-page gels (Bio-Rad Laboratories) and blotted onto 0,2 µm nitrocellulose membrane (Bio-Rad Laboratories) ...
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bioRxiv - Plant Biology 2024Quote: ... 4 µL of 10-fold diluted cDNA was combined with 5 µL of iQ SYBR Green Supermix (Bio-Rad, Cat# 170-8880) and 0.5 µL of each 10 µM forward and reverse primers ...
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bioRxiv - Neuroscience 2024Quote: ... were resolved by SDS-PAGE on 4-15% Mini-PROTEAN TGX Stain Free gels (30 μL, 10 well comb, BioRad, Cat# 4568083) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The libraries were then diluted to 10 nM and further quantitated by qPCR on a CFX Connect Real-Time qPCR system (Biorad, Hercules, CA) for accurate pooling of barcoded libraries and maximization of the number of clusters in the flow cell.
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bioRxiv - Molecular Biology 2024Quote: ... 10% Acrylamide:Bis Acrylamide) for 1 hour and transferred to a nitrocellulose membrane (0.45µm) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current for 1 hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The remaining 30 μL sample was run on a 10% acrylamide SDS-PAGE gel at 100 V for 15 minutes and stained with Coomassie (Bio-Rad #P1610786) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were boiled for 10 minutes at 95 °C and loaded onto Mini-PROTEAN TGX™ Precast Gels (BIO-RAD:4569036), and electrophoresis was performed according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... SDS-PAGE was performed using 10 μg of NP-40 extract protein mixed with an equal volume of 2X Laemmli buffer (BioRad cat#: 1610737) supplemented with 5% 2-mercaptoethanol (without boiling) ...
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bioRxiv - Molecular Biology 2023Quote: ... The denatured lysate was then clarified by centrifugation (13200 rpm, 10 min at 4°C) and protein concentration was determined using the DC™ Protein Assay (Bio-Rad). Either equal amounts or a serial dilution of the lysates from various strains were resolved using SDS-PAGE and transferred onto a polyvinylidene difluoride membrane ...
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bioRxiv - Bioengineering 2024Quote: Protein expression was confirmed by SDS-PAGE analysis using 10% precast gels (Mini-PROTEAN TGX Precast Protein Gels, Bio-Rad, Cat# 4561033). The gels were run for 55-minutes at 150V and stained with G-250 Coomassie stain (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... and 10 µg were then loaded and separated on precast 4–20% polyacrylamide Mini-PROTEAN TGX gels (Bio-Rad Laboratories, Hercules, CA). The proteins were then transferred to PVDF membranes (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted protein was subjected to an additional purification step using fast protein liquid chromatography (FPLC) and a Superdex 200 Increase 10/300 GL column (Cytiva, 28990944) in Chelex 100-treated (Bio-Rad, 1422832) dialysis buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: Equal amounts (10 μL) of the eluted solution from the streptavidin pulldown were loaded in a 4-20% polyacrylamide gel (Bio-RAD 4561096) and separated with SDS-PAGE at 100 V for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: The histones containing residue-specific acetylations or the nucleosomes used for the acetyltransferase activity assay were electrophoresed in a 10–20% sodium dodecyl sulfate polyacrylamide gel (SDS-PAGE) (DRC, NXV-396HP20) and transferred onto a nitrocellulose membrane (BIO-RAD, 1620112) at 20 V for 10 min by the semi-dry method ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were centrifuged 10 minutes at 13,000 x g and the supernatant was quantified using a DC assay kit (Bio-Rad, Hercules, California). 200 µg of total protein were diluted into a total of 1.5 mL Array Buffer and rocked over the pre-blocked array membrane containing various total RTK antibodies overnight at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were spun at 15,000 x g for 1 min in a tabletop centrifuge to pellet cell debris and the supernatant was diluted 1:10 in sterile ddH2O for a 96-well microplate Bradford microassay (Bio-Rad 5000201) to estimate the amount of leaked protein ...
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bioRxiv - Plant Biology 2022Quote: ... Leaves were kept in the dark for 10 min before luminescence was detected with a ChemiDoc XRS+ imaging system (BioRad, Feldkirchen, Germany). Luminescence intensities mm-2 were evaluated using the Image Lab software (BioRad ...
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bioRxiv - Genetics 2023Quote: ... Expression was measured with 20 ng of cDNA (calculated based on starting RNA quantity) in 10 μL reactions with SYBR Green (Bio-Rad, 1725124). Relative expression for knock-down was calculated using the Delta-Delta Ct method with Gapdh as the control reference ...
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bioRxiv - Cancer Biology 2023Quote: ... Between 40mg and 120mg of each protein sample was boiled in Laemmli buffer with 10% β-Mercaptoethanol and equal percentages of each sample were run on 4-15% polyacrylamide gels (BioRad, 4561086). Resolved proteins were wet-transferred to nitrocellulose membranes (Amersham) ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 μL of cDNA (10 ng of starting RNA) was amplified per reaction using the iTaq Universal SYBR Green Supermix (Bio-Rad, #1725124) and the Bio-Rad CFX96 qPCR system.
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bioRxiv - Biochemistry 2023Quote: ... 14 μL of Novex Hi-Density TBE Sample Buffer was added to the samples and separated in a 10% polyacrylamide non-denaturing gel (Mini-PROTEAN TGX, BIO-RAD #4561023) using 1X TBE running buffer for 30-35 min at 200 V ...
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bioRxiv - Biochemistry 2023Quote: ... cDNA was diluted 1:10 in nuclease free water prior to qPCR analysis using a CFX384 real-time PCR system (Bio-Rad, USA). Reaction mixtures containing 5 μl PowerUp SYBR Green Master Mix (Applied Biosystems ...
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bioRxiv - Biochemistry 2022Quote: ... adjusted to equal concentration for each sample and boiled at 95°C for 10 minutes in Laemmli buffer (Bio-Rad #161-0747).
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bioRxiv - Cancer Biology 2022Quote: ... The beads were washed three times with 1X PBST and resuspended in 1X Laemmli sample buffer 10% BME (Bio-Rad 161-0747).
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bioRxiv - Evolutionary Biology 2023Quote: ... purified protein fractions were separated with 10%–20% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotter (ATTO ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and 10 µl of protein sample was loaded in each well of precast Mini-PROTEAN TGX 4-20% gradient Gel (BIO-RAD 4561096). Following SDS-PAGE ...