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Citations for Bio-Rad :
2551 - 2600 of 3002 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... Protein samples were heated at 37°C for 5 min and 10 μl was loaded on SDS–polyacrylamide gel electrophoresis (PAGE) gels (4-20% Mini-PROTEAN TGX Stain-Free, Bio-Rad). After electrophoresis for 30 min at 200V ...
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bioRxiv - Biochemistry 2022Quote: ... was measured over temperatures ranging from 10 °C to 95 °C using a CFX Connect Real-Time PCR Detection System (Bio-Rad). Melting temperature (Tm ...
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bioRxiv - Biochemistry 2022Quote: ... The protein concentration was measured on Tecan (Schoeller) and approximately 20 μg of protein lysate was loaded on the 10-well protein gels (Mini-PROTEAN TGX, Bio-Rad) and run at 120 V ...
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bioRxiv - Biochemistry 2022Quote: ... Zero-minute timepoints were collected by independently adding 5 µL of each mixture to 10 µL 2X Laemmli Buffer (Bio-Rad) and boiling at 95 °C for 5 minutes ...
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bioRxiv - Bioengineering 2022Quote: The purified baculoviruses were electrophoresed on 10% sodium dodecyl sulfate-polyacrylamide gel (BIOTOOLS) and transferred to a low-fluorescence polyvinylidene fluoride (PVDF) membrane (Bio-Rad) through Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2022Quote: ... Ten to 30 μg of each sample were loaded onto 10% SDS-PAGE gels (TGX Stain-Free FastCast system, Bio-Rad), and ran at 230 V ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples were boiled with 1X Laemmli buffer containing 10% ß-mercaptoethanol before SDS-PAGE using Mini-PROTEAN TGX (Bio-Rad) pre-cast gels ...
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bioRxiv - Cell Biology 2022Quote: ... 5 µl HeLa lysate was preincubated with 1 µl of accessory protein for 10 min at room temperature in PCR plates (HSP9601, Bio-Rad). Reaction was started by adding 2 µl reaction mix ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein was resolved on 10% SDS–polyacrylamide gel electrophoresis gels and transferred onto PVDF membranes (Bio-Rad, catalog no. 1704156) by Trans-Blot Turbo (Bio-rad) ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... BP176-100) and run on a 10% SDS-PAGE using the TGX Stain-free FastCast Acrylamide Kit (Bio-Rad Cat# 1610183) and the Mini-PROTEAN system (Bio-Rad Cat# 1658006) ...
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bioRxiv - Genomics 2022Quote: ... and 20 ug of each sample were loaded on a 6% or 10% SDS-PAGE gel and transferred to a nitrocellulose membrane using Trans-Blot Turbo Transfer System (Bio-Rad). Western Blot was blocked using 5% ELK and incubated overnight with primary antibody mouse monoclonal anti-UHRF1 (Santa Cruz ...
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bioRxiv - Genetics 2022Quote: ... All samples were incubated for 10 min at room temperature in 96 well plates before imaging on a Gel doc system (Bio-Rad). The optical density (OD600 ...
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bioRxiv - Plant Biology 2022Quote: ... 0.01% [v/v] β-mercaptoethanol) was separated by 10% SDS-PAGE gel and blotted onto Immun-Blot PVDF membrane (Bio-Rad). D1 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were separated by SDS-PAGE on 10% or 4-20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... denatured for 10 min at 95 °C and loaded on a 10% SDS-polyacrylamide gel (SDS-PAGE) and separated for 45 min at 200 V (Bio-Rad). Samples were transferred to a nitrocellulose membrane (TransBlot Turbo ...
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bioRxiv - Plant Biology 2022Quote: ... Equal amount in µg from each the supernatant was loaded on a 10% (w/v) SDS-PAGE gel and transferred to a PVDF membrane (Bio-Rad). Puromycin incorporation was measured using an anti-puromycin antibody (Merk Millipore).
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bioRxiv - Microbiology 2022Quote: ... The pellet obtained was dissolved in 10 μL of 20 mM Tris-HCl (pH 7.2) and 10 μL of Laemmli sample buffer (Mini-PROTEAN® Tetra Cell-Bio-Rad). The solubilized proteins were heated (95 °C ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 μm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Microbiology 2022Quote: ... samples were incubated at 95°C for 5 or 10 min and then resolved on TGX Stain-free gel (Bio-Rad). The proteins were transferred onto 0.2 µm nitrocellulose membranes using Trans-Blot Turbo Transfer (Bio-Rad ...
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bioRxiv - Neuroscience 2022Quote: ... at either 95°C for 5 minutes or at 70°C for 10 minutes and resolved in a 4-15% Precast Gels (Bio-Rad, 12-well ...
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bioRxiv - Neuroscience 2022Quote: ... 200 µg of protein extracts were loaded and separated on a 10% SDS-PAGE gels and transferred onto PVDF membranes (Bio-Rad). Membranes were blocked with a 3% BSA in TBS-T (Tris-Buffered Saline with Tween-20 containing in mM ...
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bioRxiv - Microbiology 2023Quote: ... separated on a 10% SDS-PAGE and transferred to a PVDF membrane using the Trans-Blot® Turbo™ system (BioRad). His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr ...
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bioRxiv - Microbiology 2022Quote: ... qPCR was performed by mixing 5 μl of 5-times diluted cDNA with 10 μl of iTaq Universal SYBR Green mix (Bio-Rad), 3.6 μl of water and 0.8 μl of forward and reverse primers listed in Supplementary Table 1 ...
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bioRxiv - Molecular Biology 2023Quote: ... LAMP reaction products were analysed on 2% agarose gels (65V for 3 hr) stained with ethidium bromide (10 mg/mL) and viewed on Gel Doc XR Imaging System (BioRad, USA).
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bioRxiv - Physiology 2022Quote: 20 µg of protein were loaded and resolved on 10% TGX fast cast gels (cat#1610173, Bio-Rad, Hercules, California, USA) for 30 min at 250 V ...
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bioRxiv - Molecular Biology 2022Quote: ... A final concentration of 0.1 μg/mL DAPI was then added for 10 mins and digital images were captured using ZOE Fluorescent Cell Imager (Bio-Rad). For negative controls ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 10 min and loaded into a 4-15% gradient Tris-glycine gel (Biorad 4561084) immersed in Tris-glycine-SDS running buffer (Biorad 1610772) ...
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bioRxiv - Molecular Biology 2022Quote: ... the membranes were incubated with anti-mouse or –rabbit secondary antibodies tagged with horseradish peroxidase (1/10 000, Bio-Rad Laboratories) for one hour at room temperature and visualized by chemiluminescence (Amersham ECL prime western blotting detection reagent ...
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bioRxiv - Plant Biology 2022Quote: ... Quantitative real-time PCR was performed with 10-fold diluted cDNA using The SsoAdvancedTM Universal SYBR® Green Supermix (Bio-Rad). The mean and standard error were determined using three biological replicates with three technical replicates each ...
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bioRxiv - Cancer Biology 2024Quote: ... and 500 ng RNA was reverse transcribed into cDNA in a 10 μl reaction using the iScript cDNA synthesis kit (Bio-Rad). cDNA was then diluted 1:10 and 4 μl was used for quantitative RT-PCR using the LuminoCt SYBR Green master mix on a Roche LightCycler 480 machine ...
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bioRxiv - Biochemistry 2024Quote: Rabbit RyR1 was purified following a previously described procedure 55 and reconstituted into amphipol A8-35 by mixing at 1:10 (w/w) with RyR1 followed by overnight detergent removal with BioBeads (Bio-Rad). For the Nb selection ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were cleared by centrifugation (10 min at 14,000 x g, 4°C) and quantified by Bradford (Bio-Rad, cat#5000205). All samples were then boiled for 5 min in Laemmli sample buffer ...
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bioRxiv - Bioengineering 2024Quote: ... The samples were then loaded onto the gel and run on ice for 45 min at 150 V using 10 µL Precision Plus Protein Standards All Blue (Bio-Rad) as ladder ...
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bioRxiv - Bioengineering 2024Quote: ... 1x Dream Taq buffer A and 1 μL sample DNA in a total reaction volume of 10 uL using CFX96 Touch Real-Time PCR Detection System (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Biophysics 2024Quote: ... Liposomes were also prepared as described previously (76) and were incubated with 10% (v/v) Chelex beads (Bio-Rad, Hercules, CA) overnight at 4 °C to remove residual Ca2+ ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 2-Mercaptoethanol at 95°C for 10 min and separated by electrophoresis in Any kDTM Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... to saturate the N-terminal metal binding site,10 prior to buffer exchange using a centrifugal buffere exchange device (Bio-Spin, Bio-Rad) into 200 mM ammonium acetate supplemented with 2 x CMC of C10E5 ...
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bioRxiv - Biophysics 2024Quote: ... The DNA strands at 1 mM concentration were incubated with 10 mM of BG-GLA-NHS and reactions were cleaned up using a Bio-Spin P-30 column (Bio-rad).
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bioRxiv - Biochemistry 2024Quote: ... were resolved by SDS-PAGE (10% acrylamide) made in-house and visualized by in-gel fluorescence on a ChemiDoc MP Imaging System (Bio-Rad). The images were processed using Image Lab software (version 6.1.0).
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated on 10% or 4-20% CRITERION TGX gels under reducing conditions and transferred to Immun-Blot PVDF membranes (Bio-Rad). The membranes were blocked with 5% BSA or 5% nonfat milk in TBS-T (0.1% Tween20 ...
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bioRxiv - Cancer Biology 2024Quote: ... 40 cycles of 95 °C for 5 s and 60 °C for 10 s using a CFX Connect Real-Time PCR System (Bio-Rad). The relative expression levels of mRNA were calculated using the 2−ΔΔCt method.
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bioRxiv - Cancer Biology 2024Quote: ... 30 µg of protein lysate were then run per lane on a 10% polyacrylamide gel (Bio-Rad Laboratories, Hercules, CA, USA) and transferred to a PVDF membrane (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2024Quote: Samples in 1X Laemmli loading buffer (generally representing 1-2 µL of replication reaction or 9 µL of a plasmid pulldown sample) were resolved on 10% or 4-15% acrylamide Mini-PROTEAN or Criterion TGX precast gels (Bio-Rad) and transferred to polyvinyl difluoride (PVDF ...
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bioRxiv - Molecular Biology 2024Quote: ... or immunoprecipitated samples were resolved by SDS-PAGE (6% to 10%) and transferred onto methanol-treated immun-blot PVDF membrane (Bio-Rad). The membranes were incubated overnight at 4°C with corresponding primary antibodies (antibody information ...
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bioRxiv - Molecular Biology 2024Quote: ... a total of 20 µg whole cell lysate was resolved in a 10 % precast Mini-PROTEAN TGX Stain-Free gel (Bio-Rad). At the end of the run ...
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Plant Biology 2024Quote: ... Real-time quantitative PCR analysis was performed in a final volume of 10 μl in 96-well plates using the CFXDuet system (Bio-Rad). Amplification specificity was evaluated by post-amplification dissociation curves ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 10 min final extension at 72°C before resolving on a 5% polyacrylamide tris-borate-EDTA (TBE) gel (Bio-Rad) at 35 V ...
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bioRxiv - Molecular Biology 2024Quote: ... the membranes were washed 3 times for 10 minutes each and then developed with the Clarity or Clarity Max ECL Western Blotting Substrates (Bio-Rad). The chemiluminescence detection was performed using the Chemidoc MP system (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... Equal amounts of protein lysates (20 µg) were separated in 10% SDS-PAGE gels and transferred to PVDF membranes (Bio-Rad). The membranes were incubated with specific primary antibodies (1:1000 ...