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Citations for Bio-Rad :
2851 - 2900 of 3002 citations for 9 10 12 13 tetrahydroxyoctadecanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Surface biotinylation samples were heated to 50°C for 10 minutes before separation using 4-20% polyacrylamide gradient gels (Bio-Rad, Hercules, CA). After separation ...
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bioRxiv - Cell Biology 2021Quote: ... Proteins were separated by electrophoresis on a 10% SDS polyacrylamide gel and visualised with Silver Stain Plus Kit (Bio-Rad Laboratories AB, Sweden) according to manufacturer’s modified silver stain protocol.
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bioRxiv - Immunology 2020Quote: ... Reactions were performed in triplicates in a final reaction volume of 10 μl containing 5 μl of iQ™ SYBR® Green Supermix (Biorad, France), 4 μl of cDNA diluted 1:15 in DEPC-water and 0.5 μl of each forward and reverse primers at a final concentration of 0.5 μM (Table S2 ...
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bioRxiv - Cell Biology 2019Quote: ... A WT culture synchronized for 5% ring stages was loaded with 100 µg vector in transfection buffer via electroporation (310 V, 950 µF, 10 ms; Bio-Rad gene-pulser) as described (Ngwa et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... and equal amounts of total protein (10 to 20 µg) were loaded on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad). All samples for a participant were loaded in adjacent lanes on the same gel ...
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bioRxiv - Pathology 2019Quote: ... DNA from each specimen was pre-amplified for 10 cycles before subsequent droplet generation using the QX200 Droplet Generator (Bio-Rad Laboratories, USA)[25] ...
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bioRxiv - Biochemistry 2020Quote: Protein lysate from DENV infected and non-infected Huh7 cells (10 µg of protein as measured using Bio-Rad protein assay dye reagent) was aliquoted and purified using methanol/chloroform precipitation ...
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bioRxiv - Biochemistry 2021Quote: ... protein samples were boiled for 5 min in 1x Laemmli sample buffer and separated on an SDS-PAGE using Mini-PROTEAN® TGXTM Precast Protein Gels or 10–20% Mini-PROTEAN® Tris-Tricine Gels (Bio-Rad). Blotting was performed on a nitrocellulose membrane (GE Healthcare ...
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bioRxiv - Neuroscience 2021Quote: ... total and surface labeled proteins were separated using a 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to a PVDF membrane (Bio-Rad, Feldkirchen, Germany). The membrane was blocked for 1 h in PBS-T supplemented with 5% skim milk and afterward incubated with 1:600 primary c-Myc-tag Polyclonal antibody (sc-789 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cDNA was diluted 1:40 in water before using 1 μL as template in 10 μL qPCRs with iTaq™ Universal SYBR Green Supermix (Catalog# 1725121, Bio-Rad, USA) and 400 nM each primer ...
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bioRxiv - Microbiology 2019Quote: ... All qPCR reactions were performed in volumes of 20 μL composed of 10 μL of IQ™ SYBR® Green Supermix (Bio-Rad), 0.2 μL of each primer at 50 μM ...
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bioRxiv - Immunology 2021Quote: ... in a 1-to-1 ratio and separated by gel electrophoresis in a 10% Mini-PROTEAN TGX Precast gels (Bio-Rad Watford, UK). After gel electrophoresis ...
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bioRxiv - Microbiology 2020Quote: ... and OD600 were measured from bacterial cultures diluted 1:10 using a Synergy H1 plate reader (Bio-Tek, Winooski, WI) and a Bio-Rad spectrophotometer (Bio-Rad, Hercules, CA), respectively ...
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bioRxiv - Molecular Biology 2021Quote: PCR reactions were set up using the following volumes: 10 µL 2x ddPCR Supermix for probes (no dUTP) (BioRad Laboratories© 186-3024), 5 µL of 20plex oligo mix ...
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bioRxiv - Microbiology 2022Quote: ... 10 μg of protein was loaded from each sample into separate wells of a 4-15% polyacrylamide gel (Bio-Rad Laboratories, 456-8084). Electrophoresis was performed in 1x Tris/Glycine Buffer (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates (32 μg proteins/lane) were loaded to and separated on 10% SDS-PAGE gels and electrophoretically transferred to PVDF membrane (1620264, Bio-Rad, Hercules, CA). The membranes were blocked in 5% BSA/TBST (0.1% Tween-20 ...
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bioRxiv - Biochemistry 2022Quote: REST-FL was purified in two-step chromatography and carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... 10 ug of protein was loaded onto precast gel (4561095, 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels, Bio-Rad, USA) and resolved at 200 volts for 30-35 minutes ...
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bioRxiv - Physiology 2022Quote: Protein samples from the left ventricular heart wall or from injected Xenopus oocytes were separated by 10% SDS-PAGE and transferred onto a PVDF membrane (BioRad, Hercules, CA, USA). The primary ...
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bioRxiv - Neuroscience 2022Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min prior to loading into into a 4-15% Criterion TGX precast Midi protein gel (Bio-Rad, Cat#5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Plant Biology 2022Quote: ... was loaded onto a 10% SDS-PAGE gel and separated at 100 V for 2 h using a Mini Protean® Tetra Cell system (Bio-Rad, USA). Proteins were electro-transferred onto Immobilon TM PVDF membranes (Millipore ...
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bioRxiv - Microbiology 2023Quote: ... 5 µg (PrrF1, PrrF2) or 20 µg (PrrH) of total RNA was electrophoresed on a 10% denaturing urea TBE gel (Bio-Rad, Hercules, CA). The RNA was then transferred to a BrightStar-Plus Positively Charged Nylon Membrane (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... Ten-fold diluted cDNA samples (2 μl) were added to a mixture (10 μl total volume) containing iQ™ SYBR® Green Supermix (5 μl; Bio-Rad) and the primers of interest at a final concentration of 10 μM ...
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bioRxiv - Physiology 2022Quote: ... Primers flanking the target site were used to amplify the target region of the gene in a 10 µL polymerase chain reaction (PCR) containing: 5 µL 2 × SuperMix (Bio-Rad, Hercules, CA), 1 µL genomic DNA ...
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bioRxiv - Biochemistry 2022Quote: ... and 20 μL of the sample was mixed with 5 μL of a 5X Laemmli sample buffer containing 10% β-mercaptoethanol (Bio-Rad Laboratories). The sample was boiled for 5 min at 95 °C and loaded into a 12% polyacrylamide gel ...
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bioRxiv - Cell Biology 2024Quote: ... boiled for 5 min at 95° and resolved on 10 % or 7.5 % Mini-PROTEAN TGX Precast Gels (Bio-Rad, 4561033, 4561036, 4561023, 4561026). The SDS-PAGE was performed at 100 V for 5 minutes and then 120 V for 1 hour 15 minutes using SDS running buffer (30 g Tris ...
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bioRxiv - Microbiology 2024Quote: ... The copy number of specific transcripts was determined by analysing mouse cDNA in a 10 μl reaction mix consisting of 1x iQ SYBR® Green Supermix (Bio-Rad, USA), 0.2 μM of each primer and 2 μl of template cDNA on a CFX Connect Real-Time System (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Supernatant was collected and samples were run on 10% or 15% SDS-PAGE and analyzed by western blot using Clarity Western ECL Blotting Substrates (Bio-Rad Laboratories Inc.) and imaged using a ChemiDoc MP (Bio-Rad Laboratories Inc.) ...
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bioRxiv - Cancer Biology 2023Quote: ... Three ddPCR reactions were set up for each of the mRNA fusion assays with the following volumes: 10 µL 2x ddPCR Supermix for Probes (no dUTP) (PN 186-3023, Bio-Rad, Hercules, CA), 1 µL 20x mRNA Fusions Assay ...
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bioRxiv - Microbiology 2023Quote: Some of the affinity chromatography-purified proteins were run through a Superdex200 Increase 10/300 GL column integrated in an NGC chromatography system (Bio-Rad, CA, USA) to obtain a profile of the content in different species (aggregates ...
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bioRxiv - Genetics 2023Quote: ... was equilibrated in Milli-Q purified H2O for 10 min and then placed in the Bio-Dot Microfiltration Apparatus (Bio-Rad, 170-6545) according to manufacturer’s instructions ...
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bioRxiv - Physiology 2023Quote: ... 25 µg of total protein was loaded into precast 10 % BIO-RAD Mini-PROTEAN® TGX Stain-FreeTM SDS-polyacrylamide gel (Bio-RAD Laboratories), then electrophoretically transferred to 0.2 µm nitrocellulose membrane (Trans-Blot® TurboTM Transfer Pack ...
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bioRxiv - Biochemistry 2023Quote: Standard protocols were adopted for SDS-PAGE in 10% isocratic or 5%-15% gradient polyacrylamide mini-gels (Bio-Rad Laboratories S.r.l., Segrate, Italy), and for Western blotting by electro-transferring proteins from gels to PVDF membranes (0.2 μm pores ...
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bioRxiv - Developmental Biology 2023Quote: Proteins from worm lysates or coIP experiments were separated on 10% or 4-20% gradient Mini-PROTEAN TGX Precast Gels (Bio-Rad Laboratories, Inc.) at 300V ...
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bioRxiv - Cell Biology 2022Quote: ... in 1× TBS for 5–10 min at room temperature to visualize DNA and mounted with FluoroGuard anti-fade reagent (Bio-Rad, Hercules, CA).
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bioRxiv - Genomics 2023Quote: ... 4 μl of 1:10 dilution of cDNA was mixed with SsoAdvanced Universal SYBR Green Supermix (cat no 1725271, Bio-Rad, Hercules, CA) and 500 nM each primer up to a total volume of 10 μl and amplified in a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Synthetic Biology 2023Quote: ... The centrifuged samples (10 μL sample volume) were loaded into a 4-15% gradient Mini-Protean TGX precast protein gel (Biorad, CA, Cat # 4561086) and run at 140 V ...
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bioRxiv - Physiology 2023Quote: ... heated (70°C for 5 min) and loaded (10 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min ...
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bioRxiv - Plant Biology 2023Quote: ... reactions were prepared in a 10 µL final volume containing 5 µL of 2x SsoAdvanced™ Universal SYBR® Green Supermix (Bio-Rad), 0.125 µL of each specific primer pair at 250 nM ...
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bioRxiv - Physiology 2024Quote: ... and loaded (crude homogenate: 10 µg per lane; membrane: 2 µg per lane) onto a 7.5% polyacrylamide mini gel (Bio-Rad, Hercules, CA, USA). Proteins in the samples were separated by polyacrylamide gel electrophoresis at 200 V for 40 min and the separated proteins were transferred onto a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2024Quote: ... samples were placed on a heat block (+90°C) for 10 minutes and separated by SDS–polyacrylamide gel electrophoresis (4–20% Mini-PROTEAN TGX gels, Bio-Rad, 456-1096). Proteins were transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Genomics 2024Quote: ... 10 ng of purified genomic DNA was added to a reaction consisting of 10 µL of ddPCR Supermix for Probes (Bio-Rad, Cat #1863024), 1 µL of MseI (New England Biolabs ...
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bioRxiv - Cell Biology 2024Quote: RNA isolation and Northern blot analysis from human cultured fibroblasts were performed as previously described[82] using 10 micrograms of RNA per lane and analyzed by phosphorimager quantitation (Bio-Rad, Hercules, CA) of the resulting autoradiograph of the Northern blot (Bio-Rad ...
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bioRxiv - Bioengineering 2024Quote: ... were separated on a 4–10% gradient sodium dodecyl sulfate-polyacrylamide gel at 120 V for 90 min using a PowerPac Basic setup (Bio-Rad Laboratories, Inc.). Next ...
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bioRxiv - Cell Biology 2024Quote: ... 15 μl of homogenized lysate was used for SDS–polyacrylamide gel electrophoresis (Mini-PROTEAN® TGX™ Precast Gels, 10 wells, Bio-Rad). Proteins were transferred to a Hybond nitrocellulose membrane (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 3-10 μg total protein was loaded and run on AnykD™ Criterion™ TGX™ Precast acrylamide protein gels (Biorad cat#5671125) then transferred to nitrocellulose (Biorad cat#1704159) ...
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bioRxiv - Microbiology 2024Quote: ... followed by real-time RT-PCR targeting highly conserved regions of the IAV matrix (M) (10) using iTaq Universal Probes One-Step Kit (Bio-Rad, 1725141, USA) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: ... 10-20 µg of total protein lysate was loaded into a 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Biorad 4561095). Gels were transferred into PVDF membranes and blocked for 1 hour at room temperature in 5% BSA ...
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bioRxiv - Microbiology 2024Quote: ... Reactions were performed in a volume of 20 µL consisting of 10 µL of 2x iTAQ universial SYBR Green supermix (Bio-Rad Laboratories, USA), 2.0 µL DNA template ...