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Citations for Bio-Rad :
2451 - 2500 of 2581 citations for Anti PAK3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... the blots were incubated in and hFAB™ Rhodamine Anti-Actin (BioRad #12004163 at 1:2000 dilution). All experiments were performed in triplicate.
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bioRxiv - Neuroscience 2023Quote: ... and mouse anti-MHC Class I RT1A (#MCA51G, 1:100, clone: OX-18, 1:100, Bio-Rad). Sequential imaging was performed on an Olympus FluoView confocal laser-scanning microscope (FV1200 ...
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bioRxiv - Immunology 2022Quote: ... plates were washed and incubated with the Goat Anti-Rabbit IgG (H + L)-HRP Conjugate (Biorad, #1706515) diluted 1:2000 in BSA 1%/PBST ...
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bioRxiv - Molecular Biology 2023Quote: ... only the expected terminated and anti-terminated band intensities were quantified using Image Lab software from BIORAD by selecting the terminated band ...
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bioRxiv - Cell Biology 2024Quote: ... Surface-bound and internalized HTR-D65 were detected with HRP Goat anti-Mouse IgG (H+L) (BioRad) and o-phenylenediamine dihydrochloride (OPD ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Neuroscience 2019Quote: ... Membranes received a final wash in PBST and the antigen-antibody complex was detected by enhanced chemiluminescence using a ChemiDoc MP Imaging system (Bio-Rad Laboratories, Hercules, CA). The bands in the experimental samples were background subtracted and quantified by mean optical density using computer-assisted densitometry with Image Lab software (v4.0.1 ...
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bioRxiv - Cancer Biology 2020Quote: ... Chemiluminescent detection was performed using film or a VersaDoc CCD camera with Clarity™ Western ECL Substrate and horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, Hercules, CA) the next day ...
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bioRxiv - Developmental Biology 2023Quote: ... The membranes were washed with TBST three times and then incubated with a secondary antibody for 1 h before using Luminol/enhancer solution and ClarityTM Western ECL Substrate (Bio-Rad Laboratories, Inc. US). Western blot images were scanned by using Gel Doc XR system (Bio-Rad Laboratories ...
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bioRxiv - Physiology 2024Quote: ... for 24h for paraffin embedding. Adjacent full footprint sections were immunostained for glucagon (in-house antibody; Dr. Michael Appel) and insulin (Bio-Rad cat. 5330-0104G) using a standard ABC peroxidase protocol (Vector Labs cat ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were stained with rat monoclonal anti-BrdU [clone BU1/75 (ICR1)] (BioRad Cat #MCA 2060, RRID: AB_323427), rabbit monoclonal anti-active Caspase-3 (clone C92-605 ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) (Bio-Rad, Hercules, CA) or anti-mouse IgG-HRP conjugate (1:3000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The membranes were washed extensively with PBS-T before incubated with goat anti-mouse-HRP (Bio-Rad Laboratories) or rabbit anti-sheep-HRP (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2019Quote: ... 5 min) and incubated for 1 h at room temperature with goat anti-rabbit IgG (H+L) (BioRad). Membranes were washed and signal was detected using the ChemiDoc XRS+ (Biorad ...
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bioRxiv - Immunology 2021Quote: ... diluted 1:15000 in blocking buffer) followed by a goat anti-rabbit-HRP conjugate (170-6515, Bio-Rad, Hercules ...
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bioRxiv - Bioengineering 2020Quote: ... Isolated EVs were stained with PE-labelled anti-human TNFR1 (Bio-Rad, cat #MCA1340PE, clone H398, lot 0407) and APC-labelled anti-mouse gp130 antibodies (R&D Systems ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000) (Bio-Rad, Hercules, CA) or anti-mouse IgG-HRP conjugate (1:3000 ...
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bioRxiv - Immunology 2022Quote: ... We used mouse anti-human HLA-ABC (W6/32) conjugated to Alexa Fluor® 647 (MCA81A647, Bio-Rad), which is known to also react with MHC-I of African Green monkey from which Vero E6 cells are derived(48 ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were subsequently incubated with 0.33 µg ml-1 goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at room temperature ...
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bioRxiv - Immunology 2019Quote: ... Immunoreactive bands were visualized using horseradish peroxidase-conjugated goat anti-rabbit IgG (Bio-Rad #170-6515; 1:3000), followed by ECL detection (GE Life Sciences) ...
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bioRxiv - Neuroscience 2019Quote: ... goat anti-rat (1:1000; Alexa Fluor 568, #A11077) and V5-tag:AlexaFluor-647 (1:200; Bio-Rad MCA1360A647).
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bioRxiv - Bioengineering 2021Quote: ... Blots were subsequently incubated with 1:2000 dilution of goat-anti-rabbit or mouse horseradish peroxidase (Bio-Rad) in blocking buffer for 1 h at RT ...
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bioRxiv - Pathology 2019Quote: ... membranes were incubated with Goat Anti-Mouse IgG (Heavy + Light)-HRP conjugate (170-6516, 1:1,000; Bio-Rad) overnight and following PBS-T washing detected using the LAS-500 Imaging System (General Electric).
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bioRxiv - Cell Biology 2020Quote: ... anti-α-tubulin YL1/2 from rat (MCA77G, Bio-Rad AbD Serotec, at 1:2000 dilution for IF), anti-rabbit Alexa Fluor 488 (A11008 ...
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bioRxiv - Microbiology 2022Quote: ... The membrane was incubated with Horseradish peroxidase-conjugated (HRP-conjugated) anti-rabbit (Catalog no. 1721019; Bio-Rad, India) or anti-mouse (Catalog no ...
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bioRxiv - Microbiology 2022Quote: ... Anti-FLAG clone 1/27 was coupled to Affi-Gel 10 resin following the manufacturer’s instructions (Bio-Rad). A549 cells were inoculated at an MOI of 0.2 with S009 PB2-FLAG or S009 PB2-627K-FLAG in a 10 cm dish ...
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bioRxiv - Immunology 2023Quote: ... to block Fc receptors and stained with biotinylated anti-mouse F4/80 (clone: Cl:A3-1, cat:MCA497B, BIO-RAD), PerCP-Cy5.5-conjugated streptavidin (551419 ...
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bioRxiv - Immunology 2023Quote: ... SIgA was detected by HRP-conjugated streptavidin using biotinylated mouse anti-pig secretory component (clone K60 1F1, BioRad) diluted 1:2 000 ...
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bioRxiv - Developmental Biology 2022Quote: ... The secondary incubation was performed with goat anti-rabbit IgG-HRP conjugate (1:3000 dilution, Bio-Rad #1706515) or anti-mouse IgG-HRP conjugate (1:3000 dilution ...
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bioRxiv - Cancer Biology 2023Quote: ... the membrane was incubated with secondary goat-anti-rabbit IgG (Bio-Rad Laboratories, CA, USA; dilution 1:3000) or goat anti-mouse IgG (Bio-Rad ...
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bioRxiv - Cell Biology 2024Quote: ... and the goat anti-mouse IgG-HRP conjugate (catalog number #A-10668) was also acquired from Bio-Rad in Mississauga ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were incubated in 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 6% milk in PBS-T ...
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bioRxiv - Molecular Biology 2019Quote: ... 1% Tween-20) before incubation at room temperature for 30 minutes with a Horse Radish Peroxidase (HRP) conjugated secondary antibody (Bio-Rad 1706516 at 1:10,000) in 5% powdered milk (Bio-Rad 170-6404 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked in serum and then incubated in primary antibody for 2 hr at RT (CD-68: 1:500, Bio-Rad clone FA-11, MCA1957GA), and then processed for secondary and HRP reagent using a Vectastain ABC kit according to the manufacturer’s instructions (Rat IgG ...
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Lysyl oxidase regulates epithelial differentiation and barrier integrity in eosinophilic esophagitisbioRxiv - Cell Biology 2023Quote: ... Immunoblots were detected with an appropriate horseradish peroxidase (HRP)-conjugated secondary antibody (1:2000; NA934 or NA 931; Amersham BioSciences, Buckinghamshire, UK) by ECL detection (Bio-Rad Laboratories, Hercules, CA, USA). β-Actin served as a loading control.
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bioRxiv - Genetics 2019Quote: ... Global DNA methylation was detected using specific antibodies and was quantified colorimetrically by reading the absorbance at 450 nm in a microplate spectrophotometer (Bio-Rad Microplate Reader, Bio-Rad Laboratories, Redmond, WA, USA). The percentage of methylated DNA was proportional to the measured optical density (OD ...
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bioRxiv - Cancer Biology 2022Quote: ... membranes were incubated in horseradish peroxidase conjugated secondary antibodies for 1 hour at room temperature and finally developed using Bio-Rad ChemiDoc Imaging System (Bio-Rad, Hercules, CA, USA, cat# 12003154) using ECL substrates (ThermoFisher ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary horseradish peroxidase-coupled antibodies (1:3000, Pierce, α-mouse #1858413, α-rabbit #1858415) were applied prior to ECL-based chemiluminescence (Biorad #1705061 and ThermoFisher #34094) detected using a Fusion Fx instrument (Vilber Lourmat) ...
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bioRxiv - Cell Biology 2019Quote: ... Immunostaining for the vitronectin receptor (VNR) was with anti-CD51/61 (clone 23C6, 1:400; Bio-Rad, Oxford, UK) and for cathepsin K with a rabbit polyclonal antibody (3368-100 ...
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bioRxiv - Developmental Biology 2021Quote: ... Sections were incubated overnight at 4 °C with rabbit anti-chick IL-6 (Bio-Rad Laboratories, Inc., Hercules, CA) diluted 1:20 in incubation buffer ...
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bioRxiv - Microbiology 2019Quote: ... Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Biochemistry 2020Quote: ... His-tagged protein was detected using a mouse anti-6×His IgG conjugated to horseradish peroxidase (MCA1396P; Bio-Rad), and the blot was developed using Pierce™ ECL Western Blotting Substrate (Thermo Fisher Scientific).
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bioRxiv - Microbiology 2020Quote: Immunoreactive bands were visualized by incubation with horseradish peroxidase-conjugated goat anti-rabbit immunoglobulins (1:5000, BioRad 170-6515) or goat anti-mouse immunoglobulins (1:5000 ...
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bioRxiv - Microbiology 2020Quote: ... membranes were blocked overnight in PBST supplemented with 5% non-fat dry milk and incubated with antiserum (1:5,000) or vaginal washes (1:500) from test or control mice followed by goat anti-mouse IgG (BioRad) or IgA (SouthernBiotech ...
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bioRxiv - Neuroscience 2021Quote: ... membranes were incubated for 1 h at room temperature with peroxidase-conjugated anti-mouse (1:3000; BioRad, Hercules, CA) or anti-rabbit (1:3000 ...
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bioRxiv - Immunology 2021Quote: ... plates were washed with PBS-T and mouse anti-monkey IgA (catalog number MCA2553, Bio-Rad, Hercules, CA, USA) was added for 1 hour followed by washing with PBS-T ...
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bioRxiv - Developmental Biology 2020Quote: Streptavidin and anti-GFP beads after affinity purification were resuspended in 15 μl of 4× Laemmli sample buffer (Biorad), boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad) ...
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bioRxiv - Genomics 2019Quote: ... All blots were visualized using a goat anti-rabbit IgG:alkaline phosphatase fusion protein at 1:3,000 dilution in conjunction with BCIP/NBT color development (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... or by anti-Smc3 K112/K113 Acetylation (1:1,000, gift from Dr. Katsuhiko Shirahige) in combination with goat anti-mouse HRP (1:10,000) (Bio-Rad) and ECL prime (GE Healthcare ...