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Citations for Bio-Rad :
2301 - 2350 of 2581 citations for Anti PAK3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... incubated with specific primary antibodies for 1 h and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies by using ClarityTM Western ECL Blotting Substrate (Bio-Rad). For pulldown assays between cell extract and purified Nyx and MAG mutants ...
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A novel Hsp90 phospho-switch modulates virulence in the major human fungal pathogen Candida albicansbioRxiv - Microbiology 2020Quote: ... The blots were probed for 1 hour at room temperature with α-CaHsp9031 diluted 1:10,000 in PBST with 0.1% milk and washed with 1x PBST prior to probing with α-rabbit antibody-HRP conjugate (BioRad #1705046) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2021Quote: ... The primary antibody was removed the next day and the membrane was incubated with secondary antibody at room temperature for 1 h. Enhanced chemiluminescence substrate (Clarity Western ECL Substrate, Catalog no. 1705060) from Bio-Rad Laboratories (Mississauga ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity-ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Molecular Biology 2020Quote: Western blot detection was performed by enhanced chemiluminescence using HRP-coupled secondary antibodies and the Clarity or Clarity Max substrates (Bio-Rad). The signal was digitized using Amersham 600RGB imager as 16-bit grayscale TIFF files ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at room temperature and subsequently probed with appropriate secondary antibodies coupled to Horseradish peroxidase for 1 h at room temperature followed by chemiluminescent detection with ECL reagent (Bio-Rad).
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bioRxiv - Bioengineering 2022Quote: ... Antibody in the hybridoma supernatant was purified by passing through a column of DEAE Affigel blue (Bio-Rad, Sydney, NSW, Australia), dialysed into a Tris-saline buffer (Tris 10 mM ...
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bioRxiv - Physiology 2022Quote: ... Secondary antibodies were applied at room temperature for one hour and the blot was visualized using a ChemiDoc MP System (Bio-Rad). RBM20 bands were quantified with Image Lab software and normalized to the γ-tubulin loading control.
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bioRxiv - Physiology 2022Quote: ... diluted 1:2500 in PBS-Tween 5% milk was used to detect primary antibodies bound to the blot by BIO-RAD ChemiDoc imaging system ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by incubation with appropriate horseradish peroxidase-conjugated secondary antibodies and detection using Clarity Western enhanced chemiluminescence substrate (Bio-Rad Laboratories).
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bioRxiv - Immunology 2022Quote: ... Blocking buffer was removed and 100 µL/well of samples and IFN-γ standards (recombinant bovine IFN-γ; Bio-Rad Antibodies) starting at 300 ng/mL followed by two-fold dilutions added ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was then incubated with an HRP-conjugated secondary antibody for two hours at room temperature and developed with ClarityTM Westrern ECL substrate (Bio-Rad). Images were acquired using the ChemiDocTM imaging system (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... then incubated at RT for 1 h with HRP-linked secondary antibodies (Bio-Rad 170-6515 and 172-1011, 1:5000), imaged via chemiluminescence on a ChemiDoc Imaging System (Bio-Rad) ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with an HPR-conjugated secondary antibody and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad). Signal intensities were measured in ImageLab Software ...
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bioRxiv - Biophysics 2024Quote: ... the membrane was incubated with an HPR-conjugated secondary antibody and visualized with enhanced chemiluminescence using ChemiDoc Imaging System (Bio-Rad).
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Pyruvate kinase M2 regulates Japanese encephalitis virus replication by interacting with NS1 proteinbioRxiv - Microbiology 2024Quote: ... the membrane was washed with 1X PBS and thereafter incubated with HRP-conjugated secondary antibody for 1 hour at room temperature and detected using ECL reagent (BioRad, USA) under chemiluminescence ...
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bioRxiv - Molecular Biology 2024Quote: ... Membranes were then incubated with appropriate secondary antibodies before developing with Perkin-Elmer ECL Western Lightning Plus solution and visualized using a Chemidoc imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... blots were incubated with secondary antibodies for 1 h and were consequently developed using Clarity Western ECL and Clarity Max Western ECL substrates (Bio-Rad). Protein bands were visualized on the ChemidocTM MP imaging system (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... Appropriate secondary HRP-linked antibody was incubated for 1 hour at room temperature and developed using Clarity Western ECL blotting substrate (BioRad, 1705061).
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bioRxiv - Cancer Biology 2023Quote: ... After blocking with 5% (w/v) BSA or milk the membrane was incubated with the corresponding primary and secondary antibodies (Bio-Rad). The specific bands were analyzed using Thyphoon or ChemiDoc Imaging Systems (Bio-Rad).
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bioRxiv - Biochemistry 2023Quote: ... The membranes were subsequently probed with a primary antibody against β-ACTIN as a loading control or stained with Bio-Safe Coomassie stain (Biorad), dried ...
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bioRxiv - Microbiology 2023Quote: ... Revelation was performed using a second antibody coupled to the HRP and the Clarity Western enhanced-chemiluminescence (ECL) substrate as describes by the manufacturer (Bio-Rad). Images were acquired and analyzed with the ImageQuant Las 4000 luminescent image analyzer.
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bioRxiv - Cell Biology 2023Quote: ... before being re-incubated with the secondary antibodies at room temperatures for 2 h and then subjected to the imaging of immunoblots by Bio-Rad instrument ...
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Comprehensive mutational analysis of the checkpoint signaling function of Rpa1/Ssb1 in fission yeastbioRxiv - Genetics 2023Quote: ... the membrane was blotted with the Chk1-pS345 antibody at the 1:3000 dilution for 3 h to detect the phosphorylated Chk1-Ser345 in ChemiDoc (Bio-Rad). The membrane was stripped ...
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bioRxiv - Cell Biology 2023Quote: ... the membranes were washed three times (10 min each) and probed by incubation for 1 h with the appropriate secondary antibodies conjugated with horseradish peroxidase (Bio-Rad). The membranes were then washed three times (15 min each at room temperature) ...
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bioRxiv - Cell Biology 2023Quote: ... HRP-conjugated secondary antibodies associated signal was detected with ECL system and an enhanced chemiluminescence system (ChemiDoc Touch System, Bio-Rad). Quantification of the corresponding signal obtained was done using Image lab software.
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bioRxiv - Cancer Biology 2023Quote: ... Membranes were washed a further 3 times in TBS-T and bound antibodies were visualised using the Clarity Western ECL substrate (BioRad; #1705061) with a ChemiDoc MP system (BioRad) ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of proteins (20–100μg as optimal for each antibody) for each sample was loaded into 4-15% Mini-PROTEAN TGX Stain-Free Protein Gels (BioRad; #4568084) for electrophoresis ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibody was washed away with PBS-T and stained proteins were detected by chemiluminescence (Clarity western ECL, Bio-rad). The western blot signal was acquired using Amersham Image Quant8000.
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bioRxiv - Neuroscience 2023Quote: ... The antigen–antibody complexes were detected with SuperSignal West Pico PLUS Chemiluminescent Substrate (ThermoScientific) using the ChemiDoc XRS+ Imager System (Bio-Rad). Blots were exposed for 3 seconds ...
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bioRxiv - Pathology 2023Quote: ... HRP- conjugated secondary antibody and ECL chemical luminescence reagents were used to detect immunoblot signal in the Gel-doc system (Bio-Rad).
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bioRxiv - Cancer Biology 2023Quote: ... membranes were incubated with HRP-linked secondary antibodies and developed with Clarity or ClarityMax chemiluminescent substrates using a ChemiDoc XRS+ imager (Bio-Rad). (See Supplementary Methods Table 3 for antibody information).
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bioRxiv - Cell Biology 2023Quote: ... the membrane was incubated with primary antibodies at 4°C overnight and subsequently incubated with HRP-conjugated secondary antibodies at room temperature for 1 h and all images were acquired using a ChemiDoc XRS+ system (Bio-Rad). Mouse anti-GAPDH antibody was used at a 1:2000 dilution with TBST containing 3% BSA (Sigma) ...
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bioRxiv - Cancer Biology 2023Quote: ... PVDF membranes were washed three times with tween 20-containing tris buffered saline before incubation with HRP-conjugated secondary antibodies (Bio-Rad). Images were detected using a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Cell Biology 2023Quote: ... followed by washes at room temperature (1 x 15 min, 2 x 5 min) and incubation with HRP-conjugated secondary antibodies (Bio-Rad) for 1 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Neuroscience 2024Quote: ... The membranes were then incubated with respective primary and secondary antibodies before image acquisition using the ChemiDoc MP imaging system (Bio-Rad). Densitometry analysis was conducted using ImageJ and protein expression levels were normalized to α-tubulin loading control.
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bioRxiv - Cell Biology 2024Quote: ... Proteins were visualized using horseradish-peroxidase-conjugated secondary antibodies followed by chemiluminescence detection with ECL (Clarity Western ECL substrate, Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... CD206 was detected using rat anti-mouse CD206 (dilution 1/150, Bio-Rad, cat. MCA2235GA) and Alexa Fluor® 546 goat anti-rat antibody (dilution 1/250 ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rat Ly-6B.2 alloantigen (1:100, MCA771GA, Bio-Rad Laboratories, Hercules, CA) for neutrophils ...
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bioRxiv - Molecular Biology 2020Quote: ... and Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad, Cat NO.: 170-6516), respectively ...
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bioRxiv - Microbiology 2021Quote: ... and subsequently with Goat Anti-Mouse IgG (H + L)-HRP Conjugate (Bio-Rad, Cat. 1706516). The blot was developed with Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The slides were air dried and incubated with anti-rabies nucleocapsid conjugate (Bio-Rad, France) for 35 min at 37°C in a humid chamber and further washed with phosphate buffered saline (PBS ...
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bioRxiv - Cell Biology 2021Quote: ... Blots were then incubated in goat anti-rabbit IgG-AP conjugate (Bio-Rad, 170-6518) diluted 1:10,000 in block solution for 1 hour ...
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bioRxiv - Cancer Biology 2021Quote: ... Acid-treated fiber spreads were incubated with 1/1000 rat anti-BrdU mAb (Bio-Rad) for 1.5 hour to detect CldU ...
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bioRxiv - Microbiology 2020Quote: ... Detection of GFP was carried out with a rabbit polyclonal anti-GFP antiserum (Bio-Rad) and anti-rabbit IgG HRP-linked antibody (Cell Signalling Technology) ...
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Bat influenza vectored NS1-truncated live vaccine protects pigs against heterologous virus challengebioRxiv - Microbiology 2020Quote: ... 50 μl of horseradish peroxidase-conjugated goat anti-pig IgG (diluted 1:10000, Bio-Rad) or anti-pig IgA (diluted 1:10000 ...
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bioRxiv - Bioengineering 2019Quote: ... A 1:5000 dilution of the “Immun-Star Goat Anti-mouse-HRP conjugate” (1705047, Biorad) was used as secondary antibody under the same conditions ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... or goat anti-rabbit IgG-HRP (1:10000; #170-6515, Bio-Rad Laboratories; RRID: AB_11125142) secondary antibodies for 1h at RT ...