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Citations for Bio-Rad :
2351 - 2400 of 2581 citations for Anti PAK3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Goat anti-mouse IgG (H/L):HRP (1:1,000; multi species absorbed; Bio-Rad, STAR117P) and ECL western blot detection reagents (Cytiva ...
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bioRxiv - Plant Biology 2022Quote: ... the supernatant was incubated with anti-GFP (10 μg) immobilized on UNOsphere SUPrA beads (Biorad) at 4 °C for 2.5 hr ...
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bioRxiv - Microbiology 2024Quote: ... and anti-mouse IgG conjugated to horseradish peroxidase (HRP; Bio-Rad, Germany cat no. 1706516). Absorbance was measured at an optical density of 450 nm with a Multiskan FC plate reader (Waltham ...
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bioRxiv - Genetics 2023Quote: ... Then the blots were placed in Goat Anti-Rabbit IgG (H+L)-HRP (Bio-Rad) diluted 1:5,000 in block ...
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bioRxiv - Biophysics 2022Quote: ... UCP1 was detected using a mouse anti-pentahistidine tag:HRP 0.2 μL/mL (MCA5995P – Bio-RAD). VDAC1 Porin was used as a mitochondrial loading control and detected by a mouse anti-VDAC1 0.1 μL/mL (16G9E6BC4 - Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516, Bio-Rad; 1:2000).
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bioRxiv - Neuroscience 2023Quote: ... and then incubated with secondary anti-mouse-Starbright Blue-520 (Bio-Rad# 64456855; 1:2000) and anti-Rabbit-Starbright Blue-700 (Bio-Rad# 64484700 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-CD8 (2 µg/rat dissolved in saline solution, clone OX-8, Bio-Rad #MCA48G) or anti-rat TCRαβ antibody (2 µg/rat dissolved in saline solution ...
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bioRxiv - Cell Biology 2024Quote: ... followed by washing and incubation with HRP-conjugated goat anti-rabbit IgG Ab (Bio-Rad) for 1 hour at room temperature ...
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bioRxiv - Physiology 2024Quote: ... NE) and Starbright Blue 700 goat anti-mouse at 1:5,000 #12004159 from Bio-Rad Laboratories (Hercules ...
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bioRxiv - Microbiology 2024Quote: ... or with 0.1 µg/well of mouse anti-human IgG (CH2 domain) clone R10Z8E9 (BioRad) for Fc capture in carbonate buffer ...
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bioRxiv - Microbiology 2024Quote: ... Western blots were revealed using Goat anti-mouse IgG (H+L) HRP conjugate (Bio-Rad) and membranes were washed between the different incubations for 2 cycles of 10 minutes with TBS-T ...
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bioRxiv - Neuroscience 2021Quote: Western blotting experiments were performed on hippocampal tissue and specific bands were visualized with secondary HRP-conjugated antibodies using ChemiDoc™ Imaging Systems (Bio-Rad). The relative intensities of immunoblot bands were determined by densitometry with ImageJ software ...
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bioRxiv - Molecular Biology 2020Quote: ... The membrane was incubated with primary antibody and horseradish peroxidase–conjugated secondary antibody, and then proteins were detected using the Pierce™ ECL Western Blotting Substrate (Thermo, #32209) by BIO-RAD ChemiDoc™ XRS+ system ...
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bioRxiv - Biochemistry 2020Quote: ... The presence of the secondary antibody was confirmed using the Pierce™ECL Plus Western blotting substrate (ThermoScientific, USA) and viewed using the ChemiDoc (BioRad, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... Blotted membranes were washed three times with 0.05% Tween 20 in Tris-HCl buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against rabbit IgG (Bio-Rad Laboratories) at 1:10,000 dilution in Can Get Signal Solution 2 (TOYOBO ...
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bioRxiv - Neuroscience 2021Quote: ... The blotted membranes were rinsed three times with 0.05% Tween 20 in Tris-HCl-buffered saline (TBST) and incubated with horseradish peroxidase-conjugated goat polyclonal antibody against mouse IgG (Bio-Rad Laboratories) or guinea pig IgG (Bioss Inc. ...
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bioRxiv - Neuroscience 2021Quote: ... We obtained immunofluorescence labelling of microglia using an Iba-1 antibody (Wako, Richmond, USA) with a dilution of 1:200 co-stained with CD68 (BioRad, California, USA) with a dilution of 1:100 ...
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bioRxiv - Cancer Biology 2020Quote: ... Each PVDF-membrane piece (per antibody) was separately imaged via the auto-exposure function of the ChemiDoc-MP imaging system (Bio-Rad Laboratories). Supplementary-Fig.S4B legend describes the WB-signal quantification in detail ...
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bioRxiv - Cancer Biology 2020Quote: ... Bound IgG was visualised with horseradish peroxidase-conjugated secondary antibodies and the Clarity™ Western ECL Substrate (Cat no. 1705061, Bio-Rad, Gladesville ...
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bioRxiv - Microbiology 2021Quote: ... Goat anti-rabbit IgG HRP conjugated secondary antibody was used to detect the protein bands by chemiluminescence (Clarity ECL substrate, Cat no.170-5061, Biorad, California, USA).
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bioRxiv - Cell Biology 2022Quote: ... To verify the generation of single chain antibodies we prepared samples of whole and reduced antibodies with NuPAGE LDS Sample Buffer (4x) and run them in NuPAGE™ 7% Tris-Acetate Protein Gels (Bio-Rad) in denaturing ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Antibody binding in immunoblot analyses was detected using horse radish peroxidase-conjugated secondary antibodies and visualized using enhanced chemiluminescent chemistry (BioRad Clarity™) and visualized via Bio-Rad Chemidoc ...
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bioRxiv - Cancer Biology 2019Quote: ... Lysates were precleared with control antibody plus 30 µL of 1:1 Protein-A/G magnetic beads (BIO-RAD, Hercules, CA, USA) for 1 h at 40C ...
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bioRxiv - Microbiology 2019Quote: ... and visualized with rabbit-α-FLAG (1:3,000) primary and goat-α-rabbit-HRP conjugated secondary antibodies on the ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies were added according to manufacturer’s protocol and membranes were imaged using chemiluminescence reagent Clarity Western ECL Substrate (BioRad laboratories, Hercules, CA) and a BioRad ChemiDoc XRS system ...
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bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were visualized with goat anti-mouse or goat anti-rabbit IgG secondary antibodies (1:5000) diluted in 2% Omniblot milk (AmericanBio) in 1X TBST using a chemiluminescence detection system (BioRad ChemiDoc MP).
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bioRxiv - Plant Biology 2022Quote: ... Blots were incubated with α-TPLATE appendage antibodies (rabbit) (Dejonghe et al, 2019) and imaged on a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Cancer Biology 2024Quote: ... Complete antibody conjugation was validated by running both conjugated and un-conjugated paired antibodies on a 4-15% SDS-PAGE gel (Bio-Rad #4561083) under non-reducing conditions ...
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bioRxiv - Neuroscience 2023Quote: ... Antibody binding was detected using chemiluminescence ECL Prime Western Blotting Substrate (Cytiva) and images were acquired on ChemiDoc Touch (Bio-Rad Laboratories). The acquired images were processed and quantified using Image Lab software (Bio-Rad laboratories ...
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bioRxiv - Biochemistry 2023Quote: ... Each membrane was then incubated with the appropriate HRP-conjugated secondary antibodies in TBS-T and visualized by using the Enhanced chemiluminescence substrate (BioRad 170-5060) and ChemiDoc (BioRad).
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bioRxiv - Cancer Biology 2023Quote: ... The membrane was washed as previously mentioned and the antigen-antibody complex was revealed by Clarity Enhanced Chemiluminescence Blotting Substrate (Bio-Rad, #1705060) using the iBright™ FL100 Imaging System (ThermoFisher) ...
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bioRxiv - Immunology 2023Quote: ... Blots were probed using primary antibodies in PBST (1X DPBS with 0.05% Tween-20 (BioXtra, catalog #P7949) with 5% Blotting Grade Blocker (Bio-Rad, catalog #1706404) for approximately 16 hours (overnight ...
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bioRxiv - Microbiology 2023Quote: ... The horse-radish peroxidase conjugated to the secondary antibody was detected by incubating the membrane with 2 mL chemiluminescence substrate (Bio-Rad, 1705062) for five minutes and then imaged using a ChemiDoc XRS+ imager (Bio-Rad) ...
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bioRxiv - Neuroscience 2023Quote: ... the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies and later analyzed using a ChemiDocTM Imaging System (Bio-Rad, California, USA).
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bioRxiv - Genetics 2024Quote: ... membranes were washed three times with PBST and incubated with the appropriate StarBright Blue Fluorescent secondary antibodies (Bio-Rad 12005866 or 12004161) for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: Serum SARS2-specific binding antibody assays were performed using the Bio-Plex Pro Human SARS2 IgG 4-Plex Panel serology assay (Bio-Rad 12014634) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... CD45-APC and Bu1-PerCP-Cy5.5 were generated by conjugation of the antibody with the respective Lynx Rapid conjugation kit (Bio-Rad, Feldkirchen, Germany). Samples were analyzed on a FACS Canto II and FlowJo 10.9 software (BD ...
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bioRxiv - Molecular Biology 2019Quote: ... Both blots were incubated with goat anti-rabbit IgG-HRP conjugate (Bio-Rad, Hercules, CA, USA) diluted 1:2500 into TBS-T containing 5% nonfat milk at room temperature for 1 h followed by four 10-min washes with TBS-T ...
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bioRxiv - Bioengineering 2020Quote: ... cells were washed in PBS and were treated with anti-mouse SR-A1 (Bio-Rad Laboratories). For flow cytometric analysis of single cell suspensions prepared from harvested organs (mouse studies) ...
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bioRxiv - Neuroscience 2021Quote: ... or Goat Anti-Rabbit IgG (H L)-HRP Conjugate (1:1000; Bio-Rad; Catalog # 172-1019) in TBS-T for 1 hr at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... HRP-conjugated Goat Anti-Rabbit IgG (H+L) (1:1000, 170-6515, Bio-Rad, CA, USA) or Goat-anti-Mouse IgG (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... and 1: 25000 diluted secondary HRP conjugated Goat anti-mouse IgG (H+L) (BioRad, Mississauga, Canada). The blot was developed using Clarity Western ECL substrate (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... The plates were washed and incubated with mouse anti-His (Bio-Rad MCA-1396; 1:1000) followed by horseradish peroxidase (HRP)-conjugated goat anti-mouse secondary antibody (MerckMillipore AP124P ...
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bioRxiv - Neuroscience 2020Quote: ... Membranes were washed in TBS and further incubated with an HRP-labelled anti-rabbit IgG (BioRad) for 2h at room temperature ...
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bioRxiv - Physiology 2019Quote: ... ACPA was detected using the BioPlex® 2200 System anti-CCP reagent kit (Bio-Rad, US) and cutoff levels used were according to the manufacturer’s instructions ...
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CXCR4-binding PET tracers link monocyte recruitment and endothelial injury in murine atherosclerosisbioRxiv - Pathology 2020Quote: ... Macrophage accumulation in atherosclerosis was assessed by staining with anti-CD68 mAb (FA-11, Bio-Rad) and MOMA-2 (Bio-Rad) ...
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bioRxiv - Bioengineering 2019Quote: ... and a 1:10000 dilution of ‘Immun-Star Goat Anti-rabbit-HRP conjugate’ (170-5046, Biorad) as secondary antibody ...
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bioRxiv - Genetics 2021Quote: ... rat anti CD68 (1:200; clone FA-11/MCA1957, Bio-Rad Laboratories Inc, Hercules, CA, USA); rabbit anti LAMP1 (1:200 ...