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Citations for Bio-Rad :
2251 - 2300 of 2581 citations for Anti PAK3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... anti-rabbit (1:5000; #7074, Cell Signaling Technology or 1:10000, #12004162, Bio-Rad), actin (1:5000 ...
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bioRxiv - Neuroscience 2023Quote: ... mouse anti Human Protein Gene Product 9.5 (PGP9.5) (1:500, MCA4750GA mouse monoclonal, Biorad) for 36 h at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... either goat anti-mouse coupled with DyLight®800 (Bio-Rad, 1:10000 dilution) or goat anti-rabbit coupled with StarBright Blue 700 (Bio-rad ...
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bioRxiv - Microbiology 2023Quote: ... or goat anti-rabbit coupled with StarBright Blue 700 (Bio-rad, dilution 1:5000) and washed with PBST solution ...
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bioRxiv - Plant Biology 2024Quote: ... The goat anti-mouse IgG-HRP conjugate (cat#1706516, 1:4,000 dilution; BIO-RAD) and the secondary antibody were used to detect anti-GFP and anti-ACTIN.
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bioRxiv - Cell Biology 2024Quote: ... and HRP-conjugated donkey anti-mouse IgG (1:5000) (Bio-Rad, catalog number 1706516).
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bioRxiv - Cell Biology 2020Quote: ... Signals were developed by incubating with 1/5000 fold dilution of anti-rabbit or anti-mouse secondary antibodies for 1 h at room temperature and quantified using the Image Lab Software on a ChemiDoc XRS system (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Membranes incubated with HRP (horseradish peroxidase)–conjugated antibodies (HRP rabbit, 111 035 144; HRP mouse, 115 035 146) were developed using Clarity western ECL substrate solutions (Bio-Rad) with a ChemiDoc XRS+ imaging system (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Macrophages and T cells in aortic root were stained by incubating aortic root cross-sections with the primary antibodies to CD68 (Bio-Rad) and CD3 (Abcam) ...
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bioRxiv - Microbiology 2021Quote: ... the membranes were washed 3 times with 1 x TBS-T and antibody binding was detected in a BioRad Versa Doc System with the Quantity One software (BioRad, Germany) by chemiluminescence using the Clarity Western ECL Substrate (BioRad ...
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bioRxiv - Molecular Biology 2020Quote: ... A gate was established for each factor using negative control samples stained with mouse IgG1 negative control antibodies (BIO-RAD, MCA928) or polyclonal rabbit IgG (R&D Systems ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins of interest were detected using an HRP-conjugated secondary antibody and ECL substrates (Amersham) and visualized with the ChemiDoc MP system (Bio-Rad). Rabbit IgG heavy chains of anti-ATG12 antibody overlap with the ATG12-ATG5 conjugate ...
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bioRxiv - Cancer Biology 2019Quote: ... Appropriate secondary antibodies coupled to horseradish peroxidase were detected by enhanced chemiluminescence using Clarity™ Western ECL Blotting Substrate (BIO-Rad).
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... antigen retrieval was performed in trypsin (pH 7.8) or 10 mM sodium citrate buffer (pH 6) and then incubated with F4/80 antibody (1:50; MCA497, Bio-Rad) or UCP-1 antibody (1:500 ...
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bioRxiv - Physiology 2019Quote: ... paraffin-embedded sections were incubated at 4°C overnight with antibodies for F4/80 (MCA497G, Bio-Rad Laboratories, Hercules, CA, USA), HILPDA (sc-137518 HIG2 Antibody (C-14) ...
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bioRxiv - Neuroscience 2019Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Genetics 2019Quote: ... Global DNA methylation was detected using specific antibodies and was quantified colorimetrically by reading the absorbance at 450 nm in a microplate spectrophotometer (Bio-Rad Microplate Reader ...
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bioRxiv - Cancer Biology 2019Quote: ... transferred onto PVDF membrane and probed with primary antibody (Table 1) at a dilution of 1:1000 prepared in 5% blotting grade blocker (Bio-Rad) for 12-16 h at 4 °C ...
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bioRxiv - Bioengineering 2021Quote: ... The HRP conjugated secondary antibodies were visualized by using an enhanced chemiluminescence detection kit and ChemiDoc Touch Imaging System (Image Lab, Bio-Rad).
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bioRxiv - Cell Biology 2021Quote: ... Membranes were washed 3 times for 5 min with TBST after secondary antibody incubation and chemiluminescence signal was detected using Clarity Western ECL Substrate (Bio-Rad). All relevant information on antibodies used in this study are listed in Table S2.
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bioRxiv - Neuroscience 2021Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Cell Biology 2021Quote: ... Secondary antibody ALP conjugate (1:40,000 dilution) was also used and its blots were analysed on Gel doc system (BioRad, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... was used for chemiluminescent detection of the secondary antibody and all blots were imaged with a ChemiDoc XRS+ System (Bio-Rad).
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bioRxiv - Systems Biology 2020Quote: ... following the incubation of the membranes with the appropriate HRP-conjugated secondary antibodies and the Clarity Western ECL Substrate (Bio-Rad). The antibodies used in this study are ...
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bioRxiv - Neuroscience 2021Quote: ... blots were probed with HRP conjugated secondary antibodies and developed with Chemiluminescent substrate (Thermo) on a ChemiDoc Imaging System (Bio-Rad). Band intensity was measured with ImageJ (NIH).
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bioRxiv - Microbiology 2020Quote: ... Lysates were collected then Gag was immunoprecipitated using an α-RSV antibody and were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and quantified using a PhosphorImager (Bio-Rad). After 35S-Met/Cys labeling for 2.5-hours ...
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bioRxiv - Cell Biology 2021Quote: ... the membranes were washed 3 times for 5 min and incubated with the secondary antibodies (1:10000, IgG-HRP, Bio-Rad) for 45 min at RT ...
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bioRxiv - Microbiology 2022Quote: ... Immunoblots were probed with appropriate secondary antibodies conjugated with HRP and imaged with a ChemiDoc XRS digital imaging system (Bio-Rad).
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bioRxiv - Cell Biology 2022Quote: ... The membranes were incubated with appropriate primary and secondary antibodies listed in Table 2 in 5% milk and developed using the Clarity Western enhanced chemiluminescence substrate system (1705060, Bio-Rad) in a Xograph Compact X5 processor ...
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bioRxiv - Immunology 2020Quote: ... HRP conjugated antibodies were detected using SuperSignal West Dura Chemiluminescent Substrate (Thermo Scientific and ChemiDoc XRS and Image Lab 5.1 (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Signal detection was done with HyGLO™ quick spray chemiluminescent HRP antibody detection reagent (Denville Scientific) and Chemidoc Touch Imaging System (BioRad) and quantified by ImageJ software.
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bioRxiv - Neuroscience 2021Quote: ... analyzed by immunoblotting with the indicated antibodies and visualized using enhanced chemiluminescence (Pierce) on a Bio-Rad Versadoc 4000 (Bio-Rad).
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bioRxiv - Neuroscience 2020Quote: ... the membranes were incubated with horseradish peroxidase-conjugated secondary antibodies and later analyzed using a ChemiDoc™ Imaging System (Bio-Rad). Data is presented as ratio of phosphorylated/total protein normalized internally to each uninfected condition.
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bioRxiv - Cancer Biology 2021Quote: ... Blots were incubated with the appropriate secondary antibodies for 1 h at room temperature and visualized using the Chemi-Doc XRS+ system (Bio-Rad). Primary antibodies used were anti-BCL-XL (abcam ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed with TBS-T and incubated with HRP-linked secondary antibodies prior to detection with Clarity ECL chemiluminescence reagent (Bio-Rad). All blots were imaged on a Bio-Rad ChemiDoc-Touch system ...
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bioRxiv - Genetics 2022Quote: ... Blots with HRP-linked secondary antibodies were additionally incubated for 1-5 minutes with Clarity or Clarity Max ECL Western Blotting substrates (Bio-Rad). Blots were then imaged on a ChemiDoc MP Imaging System and analyzed with Bio-Rad Image Lab software (v5.2.1).
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bioRxiv - Cell Biology 2019Quote: ... Protein bands were visualized via enhanced chemiluminescence (Pierce ECL Western blotting substrate) using horseradish peroxidase-conjugated secondary antibodies and imaged using the ChemiDoc system (Bio-Rad). The quantifcation of the band was performed with ImageLab software (Bio-Rad ...
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bioRxiv - Plant Biology 2019Quote: ... was used at a dilution of 1:1.000 and it was visualized by incubation with goat anti-mouse IgG secondary antibodies conjugated to horseradish peroxidase (1:10.000) followed by a chemiluminescence reaction (Clarity ™ Western ECL Substrate, BIO-RAD).
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bioRxiv - Plant Biology 2020Quote: ... The membrane was incubated with secondary HRP-conjugated antibodies and signals were detected using a chemiluminescent method (ChemiDoc XRS, Bio-Rad).
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bioRxiv - Immunology 2019Quote: ... NK cells were pre-incubated with activating IB4 antibody (IgG2a, gift from Dr. Fabio Malavasi, University of Torino) blocking CD38 (clone AT13/5, Bio-Rad), CD31 (clone WM59 ...
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bioRxiv - Cancer Biology 2019Quote: ... membranes were incubated with secondary antibodies for one hour at room temperature and developed using Clarity ECL Detection Kit (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Secondary antibodies were chosen according to the species of origin of the primary antibodies and detected by an enhanced chemiluminescence system (Bio-Rad). Densitometric analysis of the relative expression of the protein of interest versus the corresponding control (β-actin or total STAT3 ...
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bioRxiv - Cell Biology 2021Quote: ... The peroxidase-conjugated secondary antibody was incubated and reacted using ECL kits (Cytiva).The signal was detected using ChemiDoc XRS + (Bio-Rad). The membrane was stripped using stripping buffer (strong ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblots were probed with appropriate secondary antibodies conjugated with HRP and imaged with a ChemiDoc XRS digital imaging system (Bio-Rad).
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bioRxiv - Cancer Biology 2020Quote: ... Primary and secondary antibodies were used as per the manufacturers’ instructions and membranes were imaged on the ChemiDoc MP (Bio-Rad) after incubation in Clarity Western ECL Substrate (Bio-Rad) ...
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bioRxiv - Immunology 2021Quote: ... 7 μm cryosections were methanol fixed for 20 minutes on ice and stained with an antibody specific to CD68 (clone: ED1, Bio-Rad). Animal protocols were approved by the University of Virginia Institutional Animal Care and Use Committee.
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bioRxiv - Biophysics 2019Quote: ... was used and the signal from HRP-conjugated secondary antibodies was detected with an ECL system and an enhanced chemiluminescence system (ChemiDoc Touch System, Bio-Rad). Quantification of the mean band intensity relative to the loading control Glyceraldehyde 3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with a secondary antibody and 1 × TBST washes the membrane was coated with chemiluminescent substrate (Clarity Max ECL substrate, Bio-Rad) and imaged on Chemidoc MP (Bio-Rad).
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bioRxiv - Microbiology 2020Quote: ... Secondary antibodies HRP-conjugated for mouse (CST 7076) or rabbit (CST 7074) were used with Clarity-ECL chemiluminescence reagent (Bio-Rad) for detection ...
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bioRxiv - Biochemistry 2021Quote: ... Antigen-primary antibodies complexes were detected with secondary antibodies conjugated to horse radish peroxidase and developed using a chemiluminescent reagent (Clarity ECL, Bio-Rad). Images of the WBs were acquired using a ChemiDoc MP System (Bio-Rad ...