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Citations for Bio-Rad :
2401 - 2450 of 2581 citations for Anti PAK3 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... After 5 washes with PBS1X-Tween 0,05% an anti-mouse IgG (H + L)-HRP Conjugate (Biorad) diluted 1:2000 in PBS1X-Tween 0,05% was added for 1h at RT ...
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bioRxiv - Microbiology 2022Quote: ... Immunoblot detection of 6xHIS and 2A peptide tags were determined through anti-Histidine tag (AD1.1.10, Biorad) and anti-2A peptide (3H4 ...
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bioRxiv - Biochemistry 2022Quote: ... resolved by LDS-PAGE and observed by immunoblot using anti-Ube2N (Bio-Rad, AHP974, 1:1000) and detected by near-infrared detection (Odyssey ...
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bioRxiv - Cell Biology 2024Quote: ... The goat anti-rabbit IgG-HRP conjugate (catalog number #17-6515) was sourced from Bio-Rad in Mississauga ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 1 hr with horseradish peroxidase conjugated anti-rabbit (BioRad 170-6515, lot #: 64033820) or anti-mouse (GE Healthcare UK Limited NA931V ...
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bioRxiv - Microbiology 2023Quote: ... followed by 1:5,000 goat anti-mouse IgG (H+L)-HRP (Bio-Rad, catalog number 1706516) in 3% milk in PBS-T and developed as described above.
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bioRxiv - Microbiology 2023Quote: ... Samples were then stained for the intracellular expression of FITC-anti-CD3 (CD3-12; MCA1477F BioRad), R718-anti-Ki-67 (B56 ...
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bioRxiv - Neuroscience 2023Quote: ... The primary Ab was followed by a secondary goat anti-rabbit Ab conjugated to HRP (BioRad) or the WFA-biotin was reacted with streptavidin-HRP (Vector Laboratories) ...
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bioRxiv - Molecular Biology 2023Quote: ... goat anti-rabbit IgG (H + L)-HRP conjugate (170-6515, Bio-Rad, CA, USA; 1:2000), and goat anti-mouse IgG (H+L)-HRP conjugate (170-6516 ...
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bioRxiv - Cell Biology 2023Quote: ... Wash three times with TBST and incubated with HRP-conjugated goat anti-rabbit IgG (Bio-Rad).
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bioRxiv - Neuroscience 2023Quote: ... anti-CD31 (rat monoclonal clone ER-MP12, #MCA2388T, RRID: AB_1101899, BioRad Laboratory; 1:200 for IHC), anti-Nestin (chicken polyclonal ...
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bioRxiv - Developmental Biology 2023Quote: ... Membranes were incubated with horseradish-peroxidase-conjugated anti-rabbit (Bio-Rad, 1721019, 1:2000, RRID: AB_11125143) or anti-mouse (SouthernBiotech ...
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bioRxiv - Genomics 2024Quote: ... the membrane was incubated with 1:10,000 dilution of HRP-conjugated anti-rabbit IgG (BioRad, 1706515) for 1 h at room temperature ...
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bioRxiv - Physiology 2024Quote: ... the blots were washed and incubated with a 1:3000 dilution of goat anti-rabbit (BioRad) or anti-mouse secondary antibodies (Bio-Rad ...
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bioRxiv - Immunology 2019Quote: ... The calculation of IgG antibody concentration against each individual influenza virus strain rHA was performed by Bio-Plex Manager™ 6.2 software (Bio-Rad Co., CA).
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bioRxiv - Cancer Biology 2020Quote: ... Membranes were rinsed 3×10 minutes in TBST 0.1% then incubated with appropriate secondary antibody coupled to the horseradish peroxidase (Biorad, 1706515, 1706516; 1/5000) for 1 hour at room temperature under agitation ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated for 2 hours at room temperature with HRP-conjugated rabbit secondary antibody (Bio-Rad, USA, 170-6515; RRID: AB_11125142) for NALCN detection and HRP-conjugated mouse secondary antibody (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... Antibodies used are listed in Supplemental table 2 Immunoreactive proteins were visualized with Clarity Western ECL Substrate (Bio-Rad Laboratories, Hercules, CA, USA). The UVP ChemStudio (Analytik Jena US LLC ...
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bioRxiv - Microbiology 2020Quote: ... MNV or HAV RNA recovered by the ISO 15216-1 and PGM-MB or HAV antibody methods was quantified using the One-Step dd-RT-PCR Advanced Kit for Probes (Bio-Rad Laboratories, Ltd.) according to manufacturer’s instructions and as described previously (23 ...
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bioRxiv - Cell Biology 2020Quote: ... Appropriate secondary antibodies coupled to horseradish peroxidase were detected by enhanced chemiluminescence using ClarityTM Western ECL Blotting Substrate (Bio-Rad, Hercules, CA, USA). Resulting protein bands were quantified and normalised to each lane’s loading control using the ImageStudio software (LI-COR).
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bioRxiv - Microbiology 2022Quote: ... were used as secondary antibodies for 1 hour at the room temperature and revealed with the addition of Clarity Western ECL reagent (Bio-Rad Laboratories Ltd). ChemiDoc MP Imaging System (Bio-Rad Laboratories Ltd ...
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bioRxiv - Plant Biology 2021Quote: ... A second polyclonal antibody conjugated with alkaline phosphatase was used for immunodetection (1:1000) at wavelength 405 nm (BioRad Microplate Reader model 680) via the substrate p-nitrophenyl-phosphate ...
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bioRxiv - Biochemistry 2021Quote: ... Immunocomplexes were visualized with horseradish peroxidase-conjugated secondary antibodies and detected with a Clarity Western ECL substrate (Bio-Rad #170-5061, Hercules, CA), and images were acquired on a chemiluminescent imager (Bio-Rad Chem-Doc Imaging System).
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bioRxiv - Developmental Biology 2019Quote: ... and incubated overnight at 4° with primary antibodies diluted in blocking solution as follows: mouse α-V5 (1:1000, SV5-Pk1, Bio-Rad #MCA1360); mouse α-SP56 (1:200 ...
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bioRxiv - Microbiology 2019Quote: ... 20 µg of capture antibody for each cytokine/chemokine type was coupled to 1.25×107 Bio-Plex Pro™ Magnetic COOH Beads (BioRad, Catalog# MC10053-01) using Bio-Plex Amine Coupling Kit (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... labelled with viability stain and surface antibodies as above and fixed and permeabilised using Leucoperm A and B reagents (Bio-Rad, Watford, UK). Cells were labelled intracellularly with antibodies (Table S2 ...
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bioRxiv - Cell Biology 2021Quote: ... Immunocomplexes were visualized with horseradish peroxidase-conjugated secondary antibodies and detected with a Clarity Western ECL substrate (Bio-Rad #170-5061, Hercules, CA), and images were acquired on a chemiluminescent imager (Bio-Rad Chem-Doc Imaging System).
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bioRxiv - Cell Biology 2021Quote: ... Appropriate secondary antibodies coupled to horseradish peroxidase were detected by enhanced chemiluminescence using ClarityTM Western ECL Blotting Substrate (Bio-Rad, Hercules, CA, USA). Resulting protein bands were quantified and normalised to each lane’s loading control using the ImageStudio Lite software (LI-COR) ...
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bioRxiv - Genetics 2022Quote: ... membranes were incubated with horseradish peroxidase-conjugated antirabbit (1: 2500, 170-6515) or antimouse (1: 5000, 170-6516) IgG secondary antibodies (BioRad, Hercules, CA, USA). Proteins were detected using the SuperSignal chemiluminescent substrate kit (Pierce).
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bioRxiv - Immunology 2022Quote: ... Both anti-IL-9 and the isotype antibody were purified from the culture supernatant of hybridoma cells using the Proteus protein purification spin kit (Bio-Rad, Hercules, CA). Anti-IL-13 is an IgG1 antibody (55 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Plates were washed again three times with TBS-T followed by the addition of primary antibodies (neat antivenom and normal horse IgG [BioRad] as negative control) in duplicate ...
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bioRxiv - Neuroscience 2024Quote: ... the membranes underwent three times with PBS-T and were then subjected to incubation with horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, CA, USA) for 1 h at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins bound by both primary and secondary antibodies were visualized by chemiluminescence after incubating the blot with Clarity Max Western ECL Blotting Substrate (Bio-Rad, Hercules, CA). Blot images were acquired on a ChemiDoc gel imaging system (Bio-Rad ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with horseradish peroxidase (HPR)-conjugated secondary antibody and visualized by chemiluminescence using a horseradish peroxidase substrate (Bio-Rad, Cat. 170-5061). Quantification was performed using ImageJ.
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bioRxiv - Cancer Biology 2023Quote: ... the membranes were incubated with respective horseradish peroxidase (HRP) conjugated secondary antibodies for two hours and imaged using ChemiDoc (Bio-Rad, California, USA) after reacting with HRP substrate ...
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bioRxiv - Neuroscience 2024Quote: ... Primary antibodies were diluted in blocking buffer (Iba1, 1:1000, Wako cat no 019-19741; CD68, 1:400, Bio-Rad cat no MCA1957) and incubated overnight at 4°C with mild agitation ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... resolved by SDS-PAGE and observed by immunoblot using rabbit anti-Ube2N (Bio-Rad, AHP974, 1:1000).
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bioRxiv - Microbiology 2021Quote: ... followed by secondary HRP- conjugated goat anti-mouse IgG(H+L) (1:10,000 dilution; Bio-Rad, USA), and then developed using the enhanced chemiluminescence procedure (Pierce ...
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bioRxiv - Microbiology 2021Quote: ... and then incubated for at least 16 hours at 4°C with either mouse monoclonal anti-His (1:2,000; AD1.1.10, Biorad) or mouse anti-RNA pol (1:5,000 ...
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bioRxiv - Cell Biology 2020Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse (#176516; 1:10,000 for Western blot) was from Bio-Rad. Alexa Fluor-conjugated goat antibodies against mouse IgG (1:500 for IF ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were washed before incubation with 1:2,500 goat anti-mouse StarBright™ Blue 700 (Bio-Rad).
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bioRxiv - Neuroscience 2021Quote: Membranes were subsequently probed with horseradish peroxidase (HRP)-conjugated goat anti-mouse or -rabbit IgG (Bio-Rad), diluted 1:5000 in 5% (w/v ...
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bioRxiv - Bioengineering 2019Quote: ... Blots were incubated the following day with 0,33μg/ml Goat-anti-rabbit or -mouse HRP (Bio-Rad) in blocking buffer for 1h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Germany) and lysosomes with rat monoclonal anti-Cd68 (clone FA-11, #MCA1957, Bio-Rad, Hercules, CA, USA). In the second set ...
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bioRxiv - Developmental Biology 2021Quote: After affinity purification anti-GFP beads were resuspended in 15 μl of 4× Laemmli sample buffer (Biorad), boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad) ...
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bioRxiv - Immunology 2021Quote: ... They were incubated overnight at 4°C with primary Ab (anti-bovine MHCII clone MCA5655 from BioRad, anti-bovine pancytokeratine clone BM4068 from Acris ...
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bioRxiv - Cell Biology 2022Quote: ... The anti-FLAG immunoprecipitates were subjected to gel electrophoresis and visualized by Oriole fluorescent gel staining (BioRad). Specific bands were excised and digested in gels with trypsin ...
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bioRxiv - Neuroscience 2023Quote: ... Western blots were performed using anti-PAR (clone #19, custom designed at Bio-Rad, AbD Serotec GmbH), anti–HK-1 (Cell Signaling ...
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bioRxiv - Immunology 2024Quote: ... Approximately 600 RUs of anti-human IgG (Cytiva) were immobilized on a GLC Sensor Chip (Bio-Rad) using amine-coupling ...