Labshake search
Citations for Bio-Rad :
1351 - 1400 of 3415 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2021Quote: ... Purified TREK-2 was then mixed with GUVs to a final concentration of ∼1 - 5 µg/ml and incubated overnight at 4 °C with 0.5mg/ml Bio-Beads (Bio-Rad) prior to use.
-
bioRxiv - Genomics 2019Quote: ... at 4 °C and transferred to a nitrocellulose membrane (0.45 μM) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current of 0.12 A for 1 □ hour ...
-
bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
-
bioRxiv - Genetics 2019Quote: ... chromosomal DNA was prepared in agarose plugs as described in (Bani Ismail et al. 2014) and run at 14 °C in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120° angle ...
-
bioRxiv - Genetics 2019Quote: ... Protein samples were denatured at 95°C for 5 min in appropriate loading buffer and loaded into precast SDS-PAGE gels (10% polyacrylamide; Biorad). Gels were run at 40 mA for 45 min in 1x running buffer (Fisher) ...
-
bioRxiv - Molecular Biology 2019Quote: ... containing 2μL of 20X reducing buffer at 95°C for 10 min and separated by SDS-PAGE short migration on 12% SDS-PAGE (Criterion XT Precast gels–BioRad). After a short migration ...
-
bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min at 100°C in SDS-PAGE sample buffer and separated on Tris-glycine 4-20% SDS-PAGE gels (BioRad). Detected protein bands for each blot were quantified using ImageJ software for gel densitometry measurements of NSP4:GAPDH.
-
bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
-
bioRxiv - Physiology 2019Quote: ... Protein lysates were prepared by centrifugation at 15,000g for 10min at 4°C and protein concentrations were calculated using Bradford assay (BioRad, UK) with BSA as a standard ...
-
bioRxiv - Cell Biology 2021Quote: Purified substrates and enzymes were centrifuged (16,000 g, 5 min, 4°C) before protein concentration was determined by Bradford assay (Bio-Rad) according to the manufacturer’s instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... Lysates were denatured by boiling in 1x Laemmli buffer at 95°C for 5 minutes and loaded on a 4–20% gradient gel (BioRad). PVDF membranes were used for proteins wet transfer (Bio-Rad) ...
-
bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
-
bioRxiv - Developmental Biology 2021Quote: ... was boiled at 95⁰C for 5 minutes prior to electrophoresis on a 4-20% gradient SDS-PAGE gel (BioRad). Membrane was blocked with 5% skim milk in TBST 1x on rocker for 1 hour ...
-
bioRxiv - Neuroscience 2021Quote: ... Samples (10 µg protein) were denatured at 95°C for 5 min in 4x Laemmli Sample Buffer (1610747, Bio-Rad) or Novex NuPAGE LDS Sample Buffer (NP0007 ...
-
bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
-
bioRxiv - Cell Biology 2020Quote: ... Lysates were cleared for 10 min at 14000 rpm at 4°C and protein concentration of the supernatant was determined by using Protein Assay (BioRad). The co-immunoprecipitation was performed using GFP trap® beads (Chromotek ...
-
bioRxiv - Biochemistry 2021Quote: ... The reaction mixture was incubated for 30 minutes at 30°C and stopped by adding 12 μl of Laemmli sample buffer (Biorad). After HMT reactions ...
-
bioRxiv - Microbiology 2021Quote: ... for 10 minutes at 37 °C and analyzed with Bio-Rad S3 Cell Sorter (Bio-Rad Laboratories Inc., California, USA) and ProSort™ Software using green fluorescence as a trigger.
-
bioRxiv - Immunology 2021Quote: Steady-state equilibrium binding of hCoV-2IG lots was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant SARS-CoV-2 RBD proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
-
bioRxiv - Genomics 2021Quote: ... centrifuged at 14,000 rcf for 20 min at 4°C and protein amounts in the supernatant determined using the Bradford assay (BioRad) according to the manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... The membrane was subsequently washed 3 times in TBS+0.05 % Tween-20 over 45 min and incubated with secondary antibodies for 1 h at RT (20 °C) after washing 3 times in TBS+0.05 %Tween-20 and developed by enhanced chemiluminescence (Bio-Rad) with Image Quant™ LAS 4000.
-
bioRxiv - Neuroscience 2021Quote: ... and equal protein amounts were boiled at 95°C for 10 minutes prior to being run on a gel (BioRad) at 120V ...
-
bioRxiv - Microbiology 2022Quote: ... as counterstain to denote stained areas and incubated at 37°C in a humidified chamber according to the manufacturer’s instructions (Bio-Rad kit 357-2112 ...
-
bioRxiv - Neuroscience 2022Quote: ... Samples were heated at 95°C for 5 minutes and loaded on 4-15% TGX gels (Bio-Rad, no. 4568083) in running buffer (25 mM Tris ...
-
bioRxiv - Cell Biology 2022Quote: ... Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) in exactly 2.4L of 0.5x Tris-borate/EDTA buffer in a Rotaphor apparatus (Biometra ...
-
bioRxiv - Molecular Biology 2022Quote: ... The mixtures were incubated for 20 min at 37°C and loaded onto a Bio-Dot Microfiltration Apparatus (Bio-Rad) loaded with nitrocellulose (0.2 μm Amersham Protran ...
-
bioRxiv - Biochemistry 2022Quote: ... Lysates were centrifuged at 14,000g for 15 min at 4°C and supernatant protein concentrations were determined by Bradford assay (Bio-Rad).
-
Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... The solution was centrifuged 16,000g for 10 minutes at 4°C and the supernatant containing the mitochondrial or cell lysate proteins quantified (Bio-Rad DC Protein Assay ...
-
bioRxiv - Immunology 2022Quote: ... and read fluorescence for 45 seconds at 60°C using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). The expression levels for each gene were then calculated per the 2-ΔΔCT equation [25].
-
bioRxiv - Genetics 2022Quote: ... Approximately 43µl of the newly formed droplet solution were transferred into a ddPCR 96-well plate and then sealed at 180°C for 5 seconds in the PX1 PCR Plate Sealer (BioRad). PCR was carried out with the following conditions ...
-
bioRxiv - Molecular Biology 2023Quote: ... PCR was achieved in 12 cycles and at optimized melting temperature (tm) of 68.2 °C thorough C1000 touch thermal cycler (Bio-rad). For single stranded PCR product (ssPCRP ...
-
bioRxiv - Neuroscience 2022Quote: ... Regional lysates were centrifuged at 21,000 x g for 20 minutes at 4 °C and the supernatant was removed and suspended in 4X laemmli buffer (BioRad) with 2-mercaptaethanol (BioRad ...
-
bioRxiv - Synthetic Biology 2022Quote: ... All reagents except the double-stranded beacon were first assembled in a total volume of 9 μL and run at 37 °C in a CFX96 real-time PCR detection system (Biorad). Both WT SpCas9 and SpCas9-NG are highly active at this temperature ...
-
bioRxiv - Molecular Biology 2022Quote: ... The reaction mixtures were held at 10°C until the PCR tubes were removed from the thermocycler (C1000 Touch, BioRad). After ...
-
bioRxiv - Molecular Biology 2022Quote: ... The plates were incubated at 30°C and pictures were taken with the setting “Blots: Colorimetric” using a ChemiDoc (BioRAD).
-
bioRxiv - Cell Biology 2022Quote: ... PIK3CA c.1633G>A (p.E545K) and PIK3CA c.1624G>A (p.E542K) point mutations were performed on the QX200 ddPCR system (Bio-Rad Laboratories) by using PrimePCR ddPCR Mutation assays according to manufacturer’s instructions (Bio-Rad Laboratories) ...
-
bioRxiv - Synthetic Biology 2022Quote: ... PFGE was undertaken by running samples on a 1.0% agarose gel in 0.5 X TBE solution at 14 °C on a Bio-Rad clamped homogeneous electric field apparatus (CHEF-DR III, BioRad). 6 V/cm were used with 15 h switch time of 60 s followed by 15 h 120 s at 120° ...
-
bioRxiv - Synthetic Biology 2023Quote: ... were cultured regularly in DMEM supplemented with 10% FBS (and 5% penicillin and streptomycinat 37°C with 5% CO2 34,35. Transfection was performed by electroporation using the Bio-Rad Gene Pulser Xcell system ...
-
bioRxiv - Molecular Biology 2023Quote: ... plates were incubated for 4d at 30°C and photographed before washing using the ChemiDoc XRS+ molecular imager (Bio-Rad) under the blot/chemicoloric setting with no filter ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The reaction was kept at 37°C and recorded using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). To prevent evaporation the plate was sealed with a transparent sticker ...
-
bioRxiv - Neuroscience 2022Quote: ... in nuclear and cytoplasmic reagents from the kit NE-PER as described by the manufacturer (Thermo Scientific).The cytoplasmic and nuclear supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
-
bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
-
bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
-
bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
-
bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
-
bioRxiv - Neuroscience 2022Quote: ... The supernatants were stored at −80°C until used and lysate protein concentration was determined using the DCTM Protein assay (Biorad).
-
bioRxiv - Biochemistry 2023Quote: ... and flash-frozen in liquid nitrogen for storage at −80°C The protein concentration was determined using the DC protein assay (BioRad) per the manufacturer’s instructions.
-
bioRxiv - Neuroscience 2023Quote: ... Proteins were separated by SDS-PAGE then transferred to nitrocellulose membrane at 4°C (0.45 μm pore size, Bio-Rad). Membranes were blocked overnight at 4° in Intercept tris-buffered saline (TBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... Lysates were cleared by centrifugation at 14,000 g for 15 min at 4°C and protein concentration was determined using the Bradford assay (Bio-Rad). Sodium dodecyl sulfate (SDS ...
-
bioRxiv - Microbiology 2023Quote: ... and 0.2M DTT) for 10 minutes at 95°C before loading in a 10% SDS-PAGE Gel (Bio-Rad 4561034). Sample proteins were separated in 1X SDS Running Buffer (25mM Tris ...