Labshake search
Citations for Bio-Rad :
1151 - 1200 of 3415 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... droplets were left at 4 °C for at least 30 minutes and read using the QX200 Droplet Reader (BioRad). ddPCR data was analyzed using QuantaSoft™ software ...
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bioRxiv - Molecular Biology 2022Quote: ... followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad). The membrane was probed using a T4 Polynucleotide Kinase (PNK ...
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bioRxiv - Cancer Biology 2022Quote: ... samples were heated for 5’ at 95°C in Lämmli buffer and protein lysates were resolved on polyacrylamide Mini-PROTEAN TGX gels (BioRad) and transferred onto nitrocellulose membrane by wet-transfer ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were then centrifuged at 12,000 rpm at 4°C for 20min and total protein extracts were boiled in Laemmli sample buffer (BioRad), separated by SDS-PAGE under reducing conditions and transferred to PVDF membranes (Millipore) ...
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bioRxiv - Microbiology 2020Quote: ... in 0.5×TBE buffer at 4 to 7 °C for 260 h using a CHEF Mapper System (Bio-Rad). Switching time was 1,200 to 4,800 s at 1.5 V/cm with an included angle of 120° ...
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bioRxiv - Molecular Biology 2020Quote: ... The immunoprecipitated proteins were eluted by heating at 100°C during 15 minutes in 4x Laemmli Sample Buffer (BIORAD). Immunoprecipitated protein complexes were subjected to Western blot analysis ...
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bioRxiv - Microbiology 2019Quote: ... the infected cell lysates were incubated for 5 min at 55°C in a S1000 thermal cycler (Bio-Rad). The total volume of each reaction was 30 μl ...
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bioRxiv - Biochemistry 2019Quote: ... Samples were run on 14% native polyacrylamide gels in TAE buffer at 4 ° C using a MiniproteanIII system (BioRad) set at 140 V ...
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bioRxiv - Microbiology 2020Quote: ... which was inoculated at 30°C for 1 hour and then photographed using a UV transilluminator (Bio-Rad, USA).
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bioRxiv - Microbiology 2021Quote: ... SOB motility plates (containing 10-4 M IPTG and 2.4 g/L MgSO4) at 37°C and pictures were taken using a Gel Doc (Biorad) imager at the beginning and the end of the linear swimming motility period (from 5 to 8 hours) ...
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bioRxiv - Cell Biology 2020Quote: Immunoprecipitated proteins were resuspended in SDS-PAGE sample buffer and heated 5 minutes at 95°C before loading onto a 7.5% acrylamide mini-Protean TGX gel (BioRad). After 7 minutes of electrophoresis at 100 V the gel was stained with Imperial Protein stain (Thermo ...
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bioRxiv - Genomics 2021Quote: ... They were then boiled for 5 minutes at 99°C after the addition of Laemmli buffer (Cat. #1410737, Biorad). Proteins were separated by SDS-PAGE and transferred to a membrane with a Trans Blot TURBO (Biorad) ...
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bioRxiv - Biochemistry 2021Quote: ... Plates were incubated ≈20-24 hours at 37°C and then photographed using a ChemiDoc XRS+ System (Bio-Rad). At least three independent transformants were used to perform replicates for phenotypic assays.
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bioRxiv - Biochemistry 2021Quote: ... the samples were gradually heated from 4 to 98°C with a rate of 0.2°C/10 s in the CFX96 Real-Time System C1000 Touch Thermal Cycler (Biorad). The fluorescence intensity was measured using an excitation wavelength of 470 nm while monitoring emission at 570 nm.
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bioRxiv - Biochemistry 2021Quote: ... The enriched protein were eluted by heating at 95°C for 30 minutes in 1X Laemmli sample buffer (BioRad) and processed to western blot ...
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bioRxiv - Genetics 2020Quote: ... 0.2 mM EDTA) at 95 °C for 30 min in a thermal cycler (BIORAD C100, BIORAD, Hercules, CA, USA), then 20 μl of neutralization buffer (40 mM TrisHCl ...
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bioRxiv - Genetics 2019Quote: ... at 4° C with 100 V for 1 hour in 1 x Tris/Glycine Buffer (Bio-Rad, Cat#1610734) and 20% methanol (Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Lysates were cleared of debris by centrifugation at 15,000 x g for 15 min at 4°C and then normalized for protein content using Bradford assay (BioRad). Normalized protein lysates were fractionated by 10 % SDS/PAGE and transferred onto a PVDF membrane (GE Healthcare Life Sciences ...
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bioRxiv - Plant Biology 2022Quote: ... 4 °C) and the supernatant was taken up and the protein concentration was determined using the Bradford assay (Biorad).
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bioRxiv - Molecular Biology 2022Quote: ... followed by cross-linking using a UV Stratalinker and drying at 80°C on a Gel Dryer (Bio-Rad).
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bioRxiv - Synthetic Biology 2022Quote: ... The reaction was run for 7 h at 34 °C on a CFX96 real-time PCR detection system (Biorad) and monitored thanks to the SYBR Green II signal.
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bioRxiv - Molecular Biology 2023Quote: ... at 95°C for 10 min and separated by electrophoresis in a 4–12% SDS–PAGE gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Molecular Biology 2022Quote: ... Plates were incubated for 18 to 24 h at 37°C and imaged in a ChemiDoc Imaging System (BioRad) to detect RFP and GFP fluorescence (Cy3 and Cy2 channels ...
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bioRxiv - Microbiology 2022Quote: ... reagent in 0.2 mL PCR 8-well strips and heated to 100 °C for 10 min (T100; BioRad, Australia) and used immediately ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluent was isolated by centrifugation at 100 g for 4 min at 4°C (Bio-Rad 732-6204), buffer exchanged (Amicon Ultra 10 kDa MWCO UFC501024 ...
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bioRxiv - Physiology 2023Quote: ... and transferred at 4°C for 16 hrs at 30 V onto 0.45 µm supported nitrocellulose membrane (Bio-rad). Membranes were blocked in EveryBlot Blocking Buffer (Bio-rad ...
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bioRxiv - Cell Biology 2023Quote: ... boiled 10 min at 95°C and run in 4% - 15% Mini-Protean TGX Stain-Free gels (Bio-Rad) in non-reducing conditions (no β-mercaptoethanol nor DTT) ...
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bioRxiv - Microbiology 2023Quote: ... 1 minute extensions and a final hold temperature of 12 C using a C1000 Touch Thermal Cycler (BioRad #1851197). Samples were subsequently loaded into the QX200 Droplet Reader for quantification ...
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bioRxiv - Microbiology 2023Quote: ... The resulting mixtures were put at 37°C for 30min and transferred to 0.4 cm electroporation cuvettes (Gene Pulser/MicroPulser, BioRad), which were submitted to electroporation using 10ms pulses at 180V repeated three times at one-second intervals ...
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bioRxiv - Molecular Biology 2023Quote: ... Total proteins were centrifuged (15000 rpm at 4°C) and determined using protein assay dye reagent (Bio-Rad, 500006). Subsequently ...
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bioRxiv - Neuroscience 2023Quote: ... Protein extracts were denatured 5 min at 95°C in a Laemmli buffer (Bio-Rad, Marnes-la-Coquette, France) with β-mercaptoethanol (1/10 ...
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bioRxiv - Neuroscience 2023Quote: ... were centrifuged at 20,000 x g for 25 min at 4⁰C and supernatants were assayed for total protein (Bradford assay, BioRad). Aliquots of WCL (30 µg total protein ...
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bioRxiv - Developmental Biology 2023Quote: ... samples were heated to 95°C with 2X sample buffer for 5 mins before loading the samples directly to a 0.2μm nitrocellulose (BioRad, #1704159) membrane ...
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bioRxiv - Synthetic Biology 2024Quote: ... followed by incubation at 37 °C for up to 7 hours on a real-time qPCR instrument (Biorad, CFX96). To measure the time-dependent change in fluorescence ...
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bioRxiv - Biochemistry 2024Quote: ... 0.1% SDS for 10 min at 60°C and images captured using a ChemiDoc MP Imaging system (BIO-RAD).
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bioRxiv - Immunology 2024Quote: ... The indicated mass of protein was boiled for 5 minutes at 95°C with 4x Laemmeli Buffer (BioRad #1610747) supplemented with 2.5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... by boiling for 10 min at 98°C before loading onto a 4-20% gel (Bio-Rad, Hercules, CA), then transferred to PVDF 0.22 m membrane (Bio-Rad ...
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bioRxiv - Molecular Biology 2023Quote: ... Pulse-field gel electrophoresis was carried out in a 0.9% agarose gel in 0.5× TBE at 14°C with a CHEF DR III from Bio-Rad with a constant switch time of 20 s during 24 h ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were centrifuged for 15 min at 15,000 x g at 4°C and the supernatant was quantified with Quick Start Bradford 1X Dye Reagent (BioRad), using an albumin curve to determine the concentration of protein to be placed ...
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bioRxiv - Cell Biology 2024Quote: ... and heated to 98°C for 5 min and transferred to Trans-Blot PVDF Transfer membranes (Bio-Rad, #1704157). Membranes were blocked for 1 h at room temperature with TBS-T (0.1% Tween 20 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 mins at 70°C and loaded onto 4-20% polyacrylamide gradient gels (Bio-Rad) for electrophoretic separation in Tris-glycine-SDS buffer (Bio-Rad) ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
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bioRxiv - Developmental Biology 2021Quote: ... membranes were incubated in 1:1000 rabbit anti-HSP90 antibody (CST #4874) and 1:5000 goat anti-rabbit HRP secondary antibody (Bio-Rad #170-5046). Signal was revealed using Clarity Max™ Western ECL Substrate (Bio-Rad #170-5062) ...
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bioRxiv - Plant Biology 2021Quote: ... The following day it was probed with 1:2000 anti-Myc primary antibody (rabbit polyclonal, Santa-Cruz, A14) and with 1:10000 mouse anti-rabbit HRP linked secondary antibody (Bio-Rad, 170-6515). Amersham ECL prime HRP substrate (GE-Lifesciences ...
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bioRxiv - Immunology 2022Quote: ELISA plates were coated with 50 µL/well of 5 µg/mL of mouse anti-bovine IFN-γ monoclonal antibody (mAb) (CC330, Bio-Rad Antibodies) by overnight incubation at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... a 1:1000 dilution of α-FLAG M2 monoclonal antibodies and/or 1:10,000 dilution of HRP-labeled α-rabbit secondary antibodies (BioRAD Catalog #170-6515) on nitrocellulose membranes ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were detected using anti-DYKDDDDK tag primary antibodies (Wako, 1E6, 1:5,000 dilution) combined with anti-mouse-HRP secondary antibodies (Bio-Rad, #1706516, 1:5,000 dilution). SigA proteins were detected using anti-SigA85 (1:10,000 dilution ...
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bioRxiv - Cell Biology 2019Quote: ... and the beads were washed with buffer #2 in Poly-Prep columns (Bio-Rad). Complexes were eluted in two fractions with buffer #2 supplemented with 2.5mM D-biotin ...
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bioRxiv - Cell Biology 2020Quote: ... and sets of gene-specific forward and reverse primers (ABCA1, CCL-2) (Bio-Rad) and subjected to real-time PCR quantification using the CFX384 Real Time PCR ...
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bioRxiv - Neuroscience 2021Quote: ... for 2 hours at room temperature and developed with ECL (Biorad 40-720-71KIT). Blots were quantified using FIJI software.