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Citations for Bio-Rad :
1601 - 1650 of 3415 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Molecular Biology 2023Quote: ... The proteins were eluted by heating the beads (95°C for 5 min.) in 70 μL of BME 4X Laemmli buffer (Biorad #1610747). Prior to loading on a 7.5% TGX SDS-PAGE gel (Biorad #5671024) ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by a 10 min final extension at 72°C before resolving on a 5% polyacrylamide tris-borate-EDTA (TBE) gel (Bio-Rad) at 35 V ...
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bioRxiv - Plant Biology 2024Quote: ... and 500nmol of each primer and annealing temperature of 59 °C on a CFX Connect Real-Time PCR Detection System (Bio-Rad) at 95 °C for two minutes followed by forty cycles of 95 °C for 15 seconds and 59 °C for 30 seconds ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were denatured at 95°C for 10 min and run on 4-20% Criterion TGX Precast Midi Protein gels (Bio-Rad). Resolved protein was transferred to nitrocellulose using Trans-Blot turbo transfer packs ...
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bioRxiv - Microbiology 2024Quote: ... 20µg of protein samples were heated in 1X Laemmli buffer at 95°C for 5 min and loaded on pre-cast 4-20% SDS-polyacrylamide electrophoresis gel (Bio-Rad). For the RIP experiment ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were centrifuged for 30 min at 20,000 x g at 4 °C and protein concentrations of the supernatants were measured with standard Bradford assay (Bio-Rad Laboratories). 25 or 50 µg of proteins were diluted in Laemmli buffer 3× (188 mM Tris-HCl ...
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bioRxiv - Microbiology 2024Quote: ... Supernatants were mixed with reducing SDS sample buffer and incubated for 5 min at 95°C prior to fractionation by SDS-PAGE analysis on 4–15 % Mini-PROTEAN TGX Stain-Free Protein Gels (Bio-Rad). Western Blot analysis was performed as described previously (15).
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bioRxiv - Biochemistry 2024Quote: ... The plates were incubated overnight at 37°C and were imaged the next day using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... Lysates were cleared by centrifugation (10 min at 14,000 x g, 4°C) and quantified by Bradford (Bio-Rad, cat#5000205). All samples were then boiled for 5 min in Laemmli sample buffer ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were boiled at 100°C for 5 min after addition of 50 µl of 4x Laemmli sample buffer (BioRad, 1610747) containing 2-Mercaptoethanol (BME) ...
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bioRxiv - Molecular Biology 2023Quote: ... containing 2-Mercaptoethanol at 95°C for 10 min and separated by electrophoresis in Any kDTM Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: ... Images were collected following 5 days of incubation at 30 °C with a Bio-Rad Chemidoc MP Imaging System (Bio-Rad) and analyzed using the ImageJ software (50).
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bioRxiv - Immunology 2024Quote: ... the proteins were transferred to a polyvinylidene difluoride membrane (110V, 1h, 4°C) using a Mini Trans-Blot cell apparatus (Bio-Rad). Non-specific binding sites were blocked using PBS/Tween 20 (0.05% Tween 20 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for either semi-dry or wet transfer (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: Samples were incubated at 95°C for 10 minutes and loaded onto SDS-polyacrylamide gels (Mini-Protean TGX gels 4-20%, Bio-Rad). Following gel electrophoresis ...
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bioRxiv - Cancer Biology 2024Quote: ... The insoluble material was separated at 13,000 rpm for 30 minutes at 4 °C and total protein concentration was measured on supernatants using Bradford reagent (Bio-Rad). Protein extracts were denatured with NuPAGE®LDS (Invitrogen ...
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bioRxiv - Plant Biology 2020Quote: ... and 2 μg of eluted RNA was used for cDNA synthesis employing iScript (Bio-Rad). Samples were analyzed using SYBR Green Supermix (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: Cell lysates were harvested by directly lysing the cells with 2 × sampling buffer (Bio-Rad). Proteins were separated by SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... 2 x 106 cKit enriched cells were resuspended in 150 µl Laemmli buffer (BioRad 1610737) supplemented with 1:10 β-mercaptoethanol ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The amplified samples were run and cut from 2% low-melt agarose gels (Bio-Rad) and followed by sRNA sequencing with NextSeq500 (Illumina ...
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bioRxiv - Neuroscience 2021Quote: ... or anti-rat Ly-6B.2 alloantigen (1:100, MCA771GA, Bio-Rad Laboratories, Hercules, CA) for neutrophils ...
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bioRxiv - Molecular Biology 2021Quote: Purified SARS-CoV-2 N protein was electrophoresed on 4-10% SDS-PAGE (Bio-Rad) and stained with Coomassie Brilliant Blue G-250 (CBBG-250) ...
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bioRxiv - Molecular Biology 2020Quote: ... The eluted fraction was precipitated with Ready Prep 2-D cleanup kit (Bio-Rad, USA). The precipitate was dissolved in O’Farrell lysis buffer and analyzed with 2D polyacrylamide gel electrophoresis ...
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bioRxiv - Plant Biology 2019Quote: ... with a 5μl reaction mixture containing 2.5μl 2× SYBR Green MasterMix (Bio-Rad, Hercules, USA), 0.25μL of both 10μM forward and reverse primers and 2μl cDNA (5ng/μl) ...
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Interaction of a sarcolipin pentamer and monomer with the sarcoplasmic reticulum calcium pump, SERCAbioRxiv - Biophysics 2019Quote: ... Detergent was slowly removed by the addition of SM-2 biobeads (Bio-Rad, Hercules, CA) over a 4-hour time course (final ratio of 25 biobeads ...
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bioRxiv - Cell Biology 2021Quote: ... Samples were transferred onto 0.2 µm (Aβ) or 0.45 µm (NUO-2) nitrocellulose membranes (BioRad) for 40 minutes at 70V ...
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bioRxiv - Biophysics 2021Quote: ... we added 60 mg Bio-beads SM-2 (Bio-Rad, Hercules, CA, USA, No. 1523920) and dialyzed the samples in detergent overnight at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted proteins were mixed with a premade 2× Sample Buffer (Biorad, Hercules, CA, USA), omitting β-mercaptoethanol (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The cell monolayers were then blocked with blocking buffer (2% Blotting-Grade Blocker [Bio-Rad] supplemented with 2% goat serum [Gibco] in PBS-Tween ...
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bioRxiv - Biochemistry 2022Quote: ... Mass calibration was done by using 2 mg/ml BSA standard (Bio-Rad, cat.5000206). PBS pH 7.4 was used as isocratic buffer ...
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bioRxiv - Physiology 2021Quote: ... The ddPCR reaction mixture consisted of 10 microliters of a 2× EvaGreen Supermix (Bio-Rad), 2 microliters of primers ...
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bioRxiv - Microbiology 2021Quote: ... Detergent was removed by incubation with 0.6 g/ml Bio-Beads SM-2 (Bio-Rad) at 4 °C for 2 hours ...
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bioRxiv - Bioengineering 2021Quote: ... samples were mixed 1:1 with 2x Laemmli sample buffer with 2-mercaptoethanol (Bio-Rad) and boiled for 10min ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 mM CaCl2) and blotted directly onto 0.45 μm pore size nitrocellulose membranes (Bio-Rad) using a BioDot SP slot-blot apparatus (BioRad ...
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bioRxiv - Molecular Biology 2019Quote: ... set at 2 copies (CNV2) on the Bio-Rad QX200 ddPCR system (Bio-Rad, CA) as per Codner and colleagues (Codner et al. ...
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bioRxiv - Biochemistry 2019Quote: ... Unilamellar proteoliposomes were spontaneously generated by removing the detergent using SM-2 Biobeads (Bio-Rad). The average radii of the prepared proteoliposomes for the proton transport assays and CD measurements were 76.87±2.29 nm and 31.46±1.68 nm ...
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bioRxiv - Cancer Biology 2020Quote: ... and transferred onto .2 um nitrocellulose membranes using the semi-dry turbo transfer system (Biorad). One hour incubation in 5% NFDM in tris-buffered saline .1% tween20 (TBST ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 μl of cDNA were analyzed using a CFX Connect real-time PCR machine (BioRad) with SensiFAST SYBR Lo-ROX Kit (Bioline) ...
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bioRxiv - Cancer Biology 2021Quote: ... Lysates were mixed 50:50 with 2X Laemmli Buffer and 2-mercaptoethanol (Bio-Rad Laboratories). Samples were run on a SDS–PAGE gel and transferred to nitrocellulose membranes before being blocked in a 10% skim milk powder/PBS solution for 60 min ...
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bioRxiv - Biochemistry 2021Quote: ... Bio-Beads SM-2 Resin and Bio-Gel P-6 were obtained from Bio-Rad Laboratories Inc ...
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bioRxiv - Cell Biology 2021Quote: For the generation of 600 pL (CV<2%) droplets at ~1,800 Hz a QX200 (BioRad) fluoro-oil flow rate of 165 μL/min was used with a total ...
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bioRxiv - Biochemistry 2020Quote: ... Detergent was slowly removed by the addition of SM-2 biobeads (Bio-Rad, Hercules, CA) over a 4-hour time course (final weight ratio of 25 biobeads to 1 detergent) ...
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bioRxiv - Genetics 2019Quote: ... Cellular proteins were denatured using Laemmli sample buffer supplemented with 2-Mercaptoethanol (BioRad, #1610747, #1610710) and heated at 95⁰C for 10 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... Beads were then washed three times with IP buffer before adding 2× Laemmli buffer (Biorad) and boiling at 95_°C for 5min ...
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bioRxiv - Biophysics 2022Quote: ... Detergents were removed by incubation with 100 mg/ml Bio-Beads SM-2 (Bio-Rad) for 1h at 23°C ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... using 1-2 ng cDNA per sample complemented with iQ SYBR®Green Mastermix (BioRad) and specific forward and reverse primers at a concentration of 0.5 µM each ...
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bioRxiv - Molecular Biology 2023Quote: ... Supernatants were collected and phosphorylated 2-(3H) deoxyglucose was separated by Poly-Prep columns (BioRad). The final eluates were mixed with Ultima Gold Scintillation fluid at a 1:7 Ratio in scintillation tubes and analysed with a beta counter.
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DNA Recognition and Induced Genome Modification by a Hydroxymethyl-γ Tail-Clamp Peptide Nucleic AcidbioRxiv - Genetics 2023Quote: ... 12.5 μl of 2 × ddPCR™ supermix for probes (no dUTP) (Bio-Rad, Hercules, CA), 0.225 μl forward primer (100 μM) ...
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bioRxiv - Genomics 2023Quote: ... 3-2) Protein Enrichment: The serum samples were processed with a ProteoMiner kit (Bio-Rad) and the protein concentration was determined using the BCA and fluorescence assays ...