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Citations for Bio-Rad :
1301 - 1350 of 3415 citations for C Type Lectin Domain Family 2 Member B CLEC2B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... and secondary antibody (anti-rabbit HRP, 1:10000, Bio-Rad) before washed and imaged (GE Amersham Imager 600).
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bioRxiv - Cell Biology 2024Quote: ... Horseradish peroxidase-conjugated secondary antibodies (Bio-Rad, 1706515 or 1706516), diluted in TBS-Tween containing 0.5% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... a rat anti-mouse CD206 primary antibody (Bio-Rad, USA), or a rat anti-mouse F4/80 primary antibody (Abcam ...
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared at 12 000 rpm for 10 min at 4°C and protein concentration adjusted with Bradford assay (BioRad). The caspase-3/7 activity of the lysates or the plated cells was analysed using Apo-ONE ® Homogenous Caspase-3/7 Assay (Promega ...
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bioRxiv - Genetics 2021Quote: ... unspecific binding sites were blocked over night at 4°C with 3% milk powder (Marvel) in Tris Buffered Saline solution (BioRad) including 1% Tween 20 (Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were boiled at 95 °C for 5 mins with 6xSDS sample buffer before loading onto 4-20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... A 1:5 dilution from serum samples was combined with 4 °C Laemmli sample buffer (Bio-Rad Laboratories, Hercules, CA) and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were separated at 175 V for 3-4 hours at 4°C and then transferred to nitrocellulose membrane (BioRad) by using a Criterion blot cell (BioRad ...
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bioRxiv - Neuroscience 2021Quote: ... Proteins were heated at 60°C and loaded on Mini-Protean TGX precast gels (456–1084, 4-15 %, Bio-Rad Laboratories ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 14,000×g for 30 min at 4°C and equalized using quick start Bradford 1x dye reagent (BioRad, 5000205). Ni-NTA resin was washed three times with lysis buffer containing 20mM imidazole pH 8.0 and 5mM β-mercaptoethanol while samples were prepared ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant from the cell lysate was collected by centrifuging the cell lysate at 10,000 g for 10 min at 4°C and further diluted in lysis buffer to 8.0 ug/ul using a modified Bradford protein quantification assay (Bio-Rad), flash-frozen in liquid N2 ...
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bioRxiv - Cell Biology 2022Quote: ... using the BioRad transfer system at 100 V for 60 min at 4°C or with Trans-Blot Turbo Mini PVDF Transfer Packs using Trans-Blot Turbo Transfer System (Biorad). The membrane was blocked for 30 min with the Odyssey Blocking Buffer (TBS ...
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bioRxiv - Plant Biology 2019Quote: Proteins were separated on denaturing SDS-PAGE followed by an electrotransfer at 4 °C onto a PVDF membrane (0.2 µM, Bio-Rad). NRT2.1 was detected using three different anti-NRT2.1 antisera produced by Eurogentec against either the synthetic peptide TLEKAGEVAKDKFGK (anti-NRT2.1 19) ...
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bioRxiv - Cancer Biology 2019Quote: ... and extension at 72°C for 30 seconds using the CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.). Analysis and fold differences were determined using the comparative threshold cycle method ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were boiled at 95 °C for 10 mins with 6×SDS sample buffer before loading onto 4–20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Cancer Biology 2019Quote: ... The eluted DNA and 10% of respective input DNA were reverse cross-linked at 65°C overnight and used for the qPCR using SYBR Green qPCR mix and a CFX96 instrument (BioRad).
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bioRxiv - Biophysics 2021Quote: ... The lysate was centrifuged at 30,000 x g for 30 min at 4°C before being loaded into a gravity flow column (Bio-Rad) and purified by Ni-NTA resin (UBP Bio ...
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bioRxiv - Genetics 2021Quote: ... temperature of hybridization 61°C) and fluorescent intensity was measured in a QX100 Droplet Reader (Bio-Rad Laboratories, Hercules, CA) and the signal data was analysed using QuantaSoft Analysis Pro (1.0.596 ...
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bioRxiv - Genetics 2021Quote: ... rotated at 4°C for 2h and then poured into a Econo-Column® Chromatography column (Bio-Rad, Hercules, CA). After extensive washing first with 80 mL of 20 mM Tris HCl [pH8@4°C] ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 30 °C and pictures were taken every day with the setting “Blots: Colorimetric” using a ChemiDoc (BioRAD).
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bioRxiv - Genetics 2020Quote: ... The reaction was incubated at 37°C for 15 min and stopped by the addition of 10 μl of TBE-UREA-sample buffer (BioRad). The samples were analysed on a 15% denaturing polyacrylamide gel in 1 × TBE buffer.
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bioRxiv - Biochemistry 2020Quote: ... The lysate was centrifuged at 30,000 x g for 30 min at 4 °C before being loaded into a gravity flow column (Bio-Rad) and purified by Ni-NTA resin (UBP Bio ...
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bioRxiv - Bioengineering 2021Quote: ... The cell pellet was re-suspended in 10mL of fresh culture media (37°C) and cells density was calculated with a TC20 automated cell counter (BioRad). 100’000 to 150’000 cells were plated per 35mm glass-bottom dish and transfected the following day ...
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bioRxiv - Bioengineering 2020Quote: ... NJ) overnight at 4°C and visualized using the Bio-Rad ChemiDoc MP Imaging System (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Biochemistry 2020Quote: ... Reactions were incubated for 15 min at 30 °C and then applied to a Bio-Gel A-1.5m (Bio-Rad) resin packed in a 0.7×15cm Flex-Column (Kimble) ...
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bioRxiv - Cell Biology 2021Quote: ... 25 µg of protein lysate was heat-denatured at 95°C for 5 minutes in Laemmli sample buffer (Bio-Rad), and then separated using a 4 to 12% Bis-Tris polyacrylamide gel (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were stored at -20°C or immediately resolved on an 7.5% SDS-PAGE pre-cast gel (Bio-Rad, #4568023) and transferred onto a 0.2 μm nitrocellulose membrane (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: Steady-state equilibrium binding of post-vaccination rabbit polyclonal serum was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant Spike proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
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bioRxiv - Neuroscience 2020Quote: ... The mixtures were incubated at 37°C in micro PCR tubes on a thermal cycler (BioRad DNA Engine PTC-200). At the indicated time points ...
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bioRxiv - Neuroscience 2020Quote: ... 15 min at +4°C) and the supernatant was used to measure the protein concentrations using the Lowry method (Biorad DC protein assay ...
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bioRxiv - Developmental Biology 2022Quote: Cells in monolayer were fixed in 4% PFA for 20 minutes at 4°C and blocked for 30 minutes in 10% donkey serum (BioRad) and 0,1% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... 7.5 mg of proteins was diluted in ChIP buffer supplemented with 0.01% SDS and precleared for 1 h at 4 °C with 50 μl of SureBeads Protein A Magnetic Beads (BioRad) and 100 μg bovine serum albumin (BSA) ...
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bioRxiv - Biochemistry 2022Quote: ... for 20 min at 4 °C) protein concentrations in supernatants collected were determined using a Bradford protein assay (Bio-Rad). Remaining supernatants were mixed with sample buffer at 3:1 v /v ratio ...
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were eluted by heating the beads at 95°C for 5 min in Laemmli Sample Buffer (1610747, Bio-Rad) with 50 mM DTT followed by magnetic separation ...
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bioRxiv - Neuroscience 2020Quote: ... 0.02% bromphenol blue) for 15min at 50°C and 5μg of protein were separated on a 4-20% SDS PAGE (Biorad, #4561096) and blotted onto nitrocellulose membranes by wet transfer ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Molecular Biology 2019Quote: ... Plates were incubated overnight at 30 °C and imaged the following day using a Gel Doc EZ Gel Documentation System (BioRad) and Image Lab (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed using SYBR Green (Applied Biosystem – 4309155) and primers listed in Table S1 at an annealing temperature of 60°C on a CFX96 thermal cycler (Biorad). Generated data were analysed using the CFX Manager Software (Biorad).
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bioRxiv - Microbiology 2020Quote: ... it was activated in an oven at 120°C and imaged using a gel doc XR image system (Bio-Rad).
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bioRxiv - Cancer Biology 2021Quote: ... centrifuged for 20 minutes at 13,000 rpm at 4°C and the protein concentration in the supernatant was measured with an DC protein assay (BioRad). Samples were separated by SDS-PAGE ...
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bioRxiv - Neuroscience 2022Quote: ... heated to 95°C for 5 min before being separated by SDS-PAGE with Criterion Precast Gels (4 –15% Tris-HCl; BioRad) and transferred onto Immun-Blot PVDF 0.2 µm (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... Lysates were then denatured in 2X Laemmli at 95°C for 5 minutes and run in Mini-PROTEAN Precast Gels (BioRad) and transferred onto membranes using Trans-Blot Turbo ...
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bioRxiv - Biophysics 2019Quote: ... Reactions were incubated at 30°C for 60 minutes and then analyzed by denaturing TBE-Urea PAGE with fluorescence detection (ChemiDoc MP, BioRad).
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bioRxiv - Developmental Biology 2019Quote: ... Homogenates were centrifuged (11,000 rpm, 10 min, 4°C) and resultant supernatant assayed using a DC Protein Assay Kit (Bio-Rad). A total of 20 μg of sample was loaded per well onto 12% SDS-PAGE gels and subsequently transferred overnight at 4°C to Immun-Blot LF PVDF membranes (Bio-Rad) ...
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bioRxiv - Biochemistry 2020Quote: ... The samples were heated at 95°C for 5 min and separated on a 12% Bis-Tris SDS-PAGE gel (Biorad).
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bioRxiv - Molecular Biology 2020Quote: ... was used at a dilution of 1:10000 for overnight at 4°C and probed for protein using ECL chemiluminescence kit (BioRad). HPLC purified protein was taken as control ...
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bioRxiv - Biochemistry 2020Quote: ... The lysates were cleared for 10 min at 21,000 × g at 4°C and Bio-Rad protein assay reagent (Bio-Rad) was used to determine the protein concentrations of lysates ...
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bioRxiv - Microbiology 2019Quote: ... The drips were allowed to dry and the plates were incubated at 37°C for 16 h and imaged using the fluorescein setting on a ChemiDoc MP Imaging machine (Biorad).
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...