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Citations for Bio-Rad :
951 - 1000 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... plates were incubated at 25 °C for 30 h before imaging on a scanner and transilluminator (Bio-Rad Gel Doc XR+).
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bioRxiv - Physiology 2023Quote: ... Samples were run 16 h at 9 °C at 4 V with a 1–10 running ratio in TBE buffer using CHEF-DRII system (Bio-Rad). After the run ...
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bioRxiv - Neuroscience 2023Quote: ... The samples were boiled for 8–10 min at 95°C and loaded onto precast 12% Bis-Tris gels (Bio-Rad) with MES SDS running buffer (Bio-Rad ...
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bioRxiv - Plant Biology 2023Quote: ... extracted proteins were diluted in SDS loading dye and incubated at 72°C for 10 min and were loaded to 4-20% Mini-PROTEAN TGX gels (Bio-Rad). Immunoblotting was performed with antibodies ...
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bioRxiv - Microbiology 2023Quote: ... Plates were incubated at 30°C and the growth was monitored by image scan using the device ChemiDoc MP (Bio-Rad) every 24 hours ...
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bioRxiv - Biochemistry 2023Quote: ... boiled for 5 min at 98 °C in the presence or absence of β-mercaptoethanol and loaded onto a TGX mini protean precast gel (Biorad) for 50 min at 200V.
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bioRxiv - Biophysics 2023Quote: ... This culture was used to inoculate 1 L of medium containing 25 µg/ml kanamycin in a 2 L baffled Erlenmeyer flask and grown at 37 °C in a C76 shaking water bath until the optical density at 600 nm (OD600, SmartSpec Plus spectrophotometer, Bio-Rad) reached ∼0.5 – 0.6 ...
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bioRxiv - Biochemistry 2023Quote: ... This mixture was then incubated at 4°C for 2 h with gentle agitation before reconstitution was initiated by removing detergent by incubating with Bio-beads (Bio-Rad) at 4°C overnight with constant rotation ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were then clarified through centrifugation (14′000g, 20 min, 4°C) and then quantified using Bradford Reagent (Bio-Rad Laboratories) according to manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... Samples were boiled for 10 min at 94°C before loading on polyacrylamide gels (4-15% Bis-Tris Gel, Bio-Rad). Proteins were separated by gel electrophoreses at 80 V and transferred at 90 V for 2 h on nitrocellulose membranes (Precast Protein Gels ...
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bioRxiv - Plant Biology 2023Quote: ... 30 s 72 °C (40 cycles) on a CFX Connect Real-Time PCR Detection System (Bio-Rad Laboratories GmbH, Munich, Germany). The annealing temperature Tm was adjusted for each primer combination ...
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bioRxiv - Systems Biology 2023Quote: ... Cell pellets were lysed by heating (95°C for 15 minutes) in a reducing buffer consisting of 90% Invitrogen Laemmli buffer (Bio-Rad) and 10% NuPAGE (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... Target amplification and real-time signal collection were carried out at 66°C for 60 mins on CFX96 Touch Real-Time PCR Detection System from Bio-Rad.
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bioRxiv - Neuroscience 2024Quote: ... via wet transfer at 30V for 1 hour at 4 °C in 10% Methanol Tris/Glycine buffer using a Criterion™ Blotter (BioRad). Blots were all blocked for 1 hour at room temperature using Intercept (TBS ...
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bioRxiv - Neuroscience 2024Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4 °C and subsequently washed in TBST and incubated for 1 hours at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Plant Biology 2024Quote: ... and 500nmol of each primer and annealing temperature of 59 °C on a CFX Connect Real-Time PCR Detection System (Bio-Rad) at 95 °C for two minutes followed by forty cycles of 95 °C for 15 seconds and 59 °C for 30 seconds ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.5% of total input and 15% of total IP fraction were resolved on 10% SDS-PAGE gels and wet transferred onto a 0.45 µm nitrocellulose membrane for 2 h at 80 V and 4°C (Bio-Rad, Germany). The membrane was blocked for 1 h in 5% BSA in PBST (PBS + 0.2% Tween20) ...
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bioRxiv - Molecular Biology 2024Quote: ... The membrane was then incubated overnight at 4°C with a 1:1000 concentration of anti-6×His tagged monoclonal antibody produced in mouse (ABD Serotec Biorad, UK). Then ...
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bioRxiv - Biochemistry 2024Quote: ... The plates were incubated overnight at 37°C and were imaged the next day using a ChemiDoc Imaging System (Bio-Rad).
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bioRxiv - Molecular Biology 2023Quote: ... containing 2-Mercaptoethanol at 95°C for 10 min and separated by electrophoresis in Any kDTM Mini-PROTEAN TGX precast gel (Bio-Rad) and transferred to a PVDF membrane (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were then transferred to a PVDF membrane at 400mA for 2 hours at 4°C in 1x Tris/Glycine buffer (1610771, Bio-Rad). Membranes were then blocked in casein blocking buffer (PI37528 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were boiled for 10 minutes at 95°C before separation on a gradient of 4-20% SDS-PAGE gels (Bio-Rad). Samples were then transferred to nitrocellulose membrane using standard methods for either semi-dry or wet transfer (Bio-Rad) ...
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bioRxiv - Biochemistry 2024Quote: Samples were incubated at 95°C for 10 minutes and loaded onto SDS-polyacrylamide gels (Mini-Protean TGX gels 4-20%, Bio-Rad). Following gel electrophoresis ...
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bioRxiv - Immunology 2021Quote: ... Proteins were also run on a sodium dodecyl sulphate (SDS) polyacrylamide gels (5–20% gradient; Bio-Rad) to check for purity39,40 ...
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bioRxiv - Developmental Biology 2019Quote: PVDF membranes carrying proteins were incubated in the TBST buffer with 5% nonfat dry milk (BIO-RAD, Blotting-Grade Blocker ...
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bioRxiv - Neuroscience 2020Quote: ... Denatured protein lysates (5 μg) were loaded in 4–20% Criterion TGX Precast Gels (Bio-Rad, 5678095) and transferred to Immobilon-FL PVDF membrane (0.45 μm pore ...
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bioRxiv - Genetics 2023Quote: Western blot – The different protein lysates were fractionated on 5-12% SDS-PAGE precast gels (Bio-Rad) and transferred onto Trans-Blot Turbo Mini 0.2 µm PVDF membranes using the Trans-Blot Turbo Transfer System (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: 25µg of protein lysates were denatured at 95℃ for 5 minutes in 4x Laemmli Sample Buffer (BioRad) containing β-Mercaptoethanol (Millipore Sigma) ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Immunology 2021Quote: Recombinant human GFAP protein were run on 12% SDS-PAGE and transferred onto membrane via Trans-Blot Turbo Transfer System (BIO-RAD). Membrane was incubated in blocking buffer (PBS ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second serum panel (n=29 ...
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bioRxiv - Molecular Biology 2019Quote: The SPR studies to determine conformation binding of the human chimeric 1A8 and 7C8 mAbs were carried out with the ProteOn XPR36 protein interaction array system (Bio-Rad) based on SPR technology ...
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bioRxiv - Microbiology 2022Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 29 sera collected from individuals 1 month after BA.5-bivalent-booster of Pfizer or Moderna vaccine ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were collected from human or mouse brain homogenates after centrifugation and the protein concentration was measured by Bradford Assay (Bio-Rad). Equal amounts of proteins were run on a 4-12% gradient SDS-PAGE gel (Invitrogen ...
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bioRxiv - Microbiology 2023Quote: ... This panel had been tested negative for SARS-CoV-2 nucleocapsid protein expression using Bio-Plex Pro Human IgG SARS-CoV-2 N/RBD/S1/S2 4-Plex Panel (Bio-rad). The second panel consisted of 20 sera from individuals who were previously infected by SARS-CoV-2 vaccinated with 2-4 doses of parental mRNA vaccine ...
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bioRxiv - Biochemistry 2021Quote: ... were co-electroporated with 1.5 x 107 KH2 ES cells at 400 V and 125 μf using two pulses in a Gene PulserII (Bio-Rad, Hercules, CA). Recombination between FRT sites such that the entire plasmid is inserted within the PGK promoter and the ATG initiation codon upstream and in frame with the hygromycin resistance gene was used to select correctly targeted cells ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Neuroscience 2024Quote: ... The slides were then incubated for 60 minutes at room temperature in blocking buffer (PBS with 5 % goat serum [Abcam #ab7481] and 0.3 % Triton X-100 [Bio-Rad Laboratories #161-0407]). After removing the blocking buffer and re-applying PAP pen ...
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bioRxiv - Cell Biology 2022Quote: ... Triton X-100 (Bio-Rad) used at 1% for 10 min at RT allowed cell permeabilization and a solution of bovine serum albumin (BSA ...
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bioRxiv - Cell Biology 2019Quote: ... lysates were centrifuged at 16,000 x g to pellet cellular debris and protein concentration was determined by BCA quantification assay (Bio-Rad, Hercules, CA, USA). For each sample ...
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bioRxiv - Plant Biology 2021Quote: ... Membranes were washed 3 x 10min in TBS + Tween 20 before proteins were detected by Immuno-Star luminol-peroxide (Bio-Rad, #170-5070). Bio-Rad Chemi-doc was used to visualize and quantify proteins with densitometry.
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bioRxiv - Immunology 2023Quote: ... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
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bioRxiv - Developmental Biology 2021Quote: ... Additional pre-absorption controls were performed in which the anti-IL-6 antibody was incubated overnight at 4 °C with a 10 fold molar excess of recombinant chicken IL-6 (1.67 μM; Bio-Rad Laboratories, Inc.) before immunolabeling fixed sections of chick ocular tissues ...
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bioRxiv - Neuroscience 2021Quote: ... Total RNA was purified with DNase and incubated at 37°C for 30 minutes using a thermocycler (Bio-Rad, Hercules, CA, USA). cDNA was synthesized using an iScript kit (Bio-Rad) ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by 40 cycles of denaturation at 95°C for 10 seconds and extension at 60°C for 30 seconds on the Bio-Rad CFX-96 qPCR machine (Bio-Rad, UK). Data were analysed with CFX-manager software v.3.1 ...
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bioRxiv - Zoology 2019Quote: ... followed by extension at 72°C for 10 min in a T100™ Thermal Cycler (Bio-Rad Laboratories, Inc. Hercules, CA, USA). PCR products were run against a Genescan™ 500 LIZ™ internal size standard on an ABI 3130 Genetic Analyzer (Applied Biosystems ...
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bioRxiv - Synthetic Biology 2020Quote: ... and MgCl2 [12.5 mM]) and annealed by cooling the solution from 95 to 12 °C over 30 min in a thermal cycler (Bio-Rad, T100). Detailed descriptions for the assembly of individual gate complexes are described in the supplementary information ...
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bioRxiv - Biophysics 2020Quote: ... 50 µl of a 10− 6 M biotinylated and 10−7 M dye-coupled oligonucleotide solution were co-incubated to anneal the oligonucleotides at 80 °C using a Thermocycler (BioRad, C1000 ThermoCycler), followed by sample cooling to 4 °C with -1 °C/min ...
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bioRxiv - Genomics 2021Quote: ... at 4°C in 1×TAE buffer (40 mM Tris-acetate, 1 mM EDTA) using 0.55% Certified Megabase agarose gel (Bio-Rad, 161-3109). Short-range PFGE ran at 4.2 or 4.5 V/cm with a pulse time from 40 to 70 s for 24 h ...
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bioRxiv - Immunology 2021Quote: ... The cells were centrifuged at 300G for 7 minutes at 4°C and the cell pellet was resuspended then counted with the BioRad cell counter (Bio-Rad, TC20).