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Citations for Bio-Rad :
1001 - 1050 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: Binding kinetic of the purified Fabs to full length PrP was monitored at 25 °C using ProteOn XPR36 surface plasmon resonance biosensor (Bio-Rad Laboratories). Mouse recPrP23–231 was immobilized on a carboxymethylated-dextran sensor chip (CM5 ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad) and visualized with GelCode Blue stain (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... Selected siRNAs were spotted onto a gridded MatTek dish (P35G-2-14-C-GRID) using a contact spotter (ChipWriter Pro-Bio-Rad Laboratories) resulting in a layout of 4 × 8 spots 40 ...
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bioRxiv - Microbiology 2022Quote: ... The melt curve analysis was performed at 65°C for 1 minute in CFX connects TM Real-time PCR detection system (Bio-Rad, USA). The primers used are listed inTable 1 ...
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bioRxiv - Neuroscience 2022Quote: ... and heated at 50 °C for 30 min prior to loading on a 10% criterion Tri-HCl gel (Bio-Rad, Hercules, CA). Samples were then transferred onto PVDF membranes (Millipore Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... The RPA mix was incubated at 39 °C when not mentioned otherwise and was monitored in real-time (Bio-Rad, CFX96 Touch). Optimized qeRPA reaction consists of one RPA pellet resuspended to a final volume of 100 μl with 300 nM primers ...
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bioRxiv - Microbiology 2021Quote: ... Samples were electrophoretically resolved on gelatin-containing gels at 125 V for 110 min at 4 °C using a Power Pack supply (Bio-Rad, USA) Next ...
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bioRxiv - Microbiology 2020Quote: ... The suspension was mixed on an orbital shaker for 60 – 90 minutes at 4 °C and then transferred to a glass column (BIO-RAD EconoColumn). Non-His-Tagged proteins were eluted with lysis buffer until the absorbance at 280 nm matched background levels utilizing 50 – 100 mL of lysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... the plates were incubated at 37°C for 24 h in ambient air and growth was assessed at OD595 using microplate reader (iMark, Bio-Rad, Japan). The lowest concentration that completely prevented bacterial growth was categorized as MIC.
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bioRxiv - Biochemistry 2020Quote: ... N-glycans were reduced with 1 M NaBH4 in 50 mM KOH for 3 h at 50°C then desalted and enriched offline using AG 50W-X8 (Bio-Rad, Australia) strong cation exchange followed by porous graphitized carbon (PGC ...
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bioRxiv - Molecular Biology 2022Quote: ... The synthetic ssDNA oligo was converted to dsDNA by primer extension reaction at 60° C using iProof DNA polymerase (Bio-Rad, USA). The resulting duplex was cloned between the EcoRI and HindIII sites of the pUC57 vector ...
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bioRxiv - Neuroscience 2019Quote: ... and samples were then transferred at 100 V at 4°C onto an activated 2 um polyvinylidene difluoride (PVDF) membrane (Bio-Rad 1620177), blocked with 3% BSA in 0.2% Tween-20 (Sigma P9614 ...
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bioRxiv - Microbiology 2019Quote: ... followed by 40 cycles at 95°C for 10 seconds and 60°C for 30 seconds in a Bio-Rad CFX96™ Real-Time System coupled to a C1000™ Thermal Cycler (BioRad). Expression of target genes was normalized by the β-actin endogenous transcript levels for each RNA prevenient from the culture conditions [47] ...
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bioRxiv - Developmental Biology 2019Quote: Oocytes were collected in 0.1% PVP in DPBS and boiled for 3 mins at 95°C in 1x Laemmli Sample Buffer (Bio-Rad, 161-0747) supplemented with β-mercaptoethanol ...
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bioRxiv - Microbiology 2019Quote: ... pH 8 for 1 h at 37°C and then loaded into the wells of 1% pulse-field certified agarose gel (Bio-Rad Laboratories) in 0.5X TBE (45mM Tris HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... The protein samples were combined with the 4x Laemli sample buffer (900 μl of sample buffer and 100 μl β-Mercaptoethanol), heated (95°C, 5mins) and run on SDS-PAGE (protean TGX pre-casted gradient gel, 4%-20%, Bio-rad) and transferred to the Nitro-Cellulose membrane (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... Final extension was at 72°C for 7min and the reaction was stopped by holding tubes at 4°C until removed from the thermal cycler (MyCycler™ Thermal Cycler BIORAD, USA). The PCR products were electrophoresed in a 1.5% agarose gel stained with SYBR Safe DNA Gel stain (Invitrogen ...
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bioRxiv - Bioengineering 2020Quote: ... the 96-well microplate was incubated at 37 °C for 1h and the end-point image was taken by ChemiDoc™ MP Imaging System (Bio-Rad).
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bioRxiv - Bioengineering 2020Quote: ... The DAMR systtem for one-pot RPA/CRISPR-Cas12a detection was incubated in PCR tubes at 37 °C and the real-time fluorescence signal was monitored by CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad). After reaction ...
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bioRxiv - Biochemistry 2021Quote: ... The membranes were incubated in the dark with the CSPD reagent for 30 min at 37 °C before imaging with ChemiDoc™ MP Imaging System (BIO-RAD). tRNA-Glu northern blot was synthesized by IDT and the sequence was listed below ...
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bioRxiv - Immunology 2019Quote: ... Upfront tagmentation of ~35,000 nuclei was performed for 1 h at 37°C in a C1000 Touch thermal cycler with 96-Deep Well Reaction Module (Bio-Rad, 1851197). Tagmented nuclei were mixed the master mix and loaded onto a Chromium Chip with Gel beads and partitioning oil on different wells ...
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bioRxiv - Cell Biology 2019Quote: ... The reaction was quenched by incubation with 1 mM TCEP for 20 minutes at 37°C and 60% of the eluate was run on an SDS-PAGE gel (Mini-Protean TGX, 4-15%, Bio-Rad #4561084). The gel was stained with colloidal Coomassie (Bio-Rad #1610803 ...
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bioRxiv - Bioengineering 2022Quote: ... pH 8.3 at 100 V for 2 h at 2-8 °C using PowerPac Basic setup (Bio-Rad Laboratories Inc., Hercules, CA). Post-electrophoresis ...
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bioRxiv - Neuroscience 2022Quote: ... while brain cytokine analyses for Studies C and D were conducted using a custom 10-plex Bioplex (Bio-rad Laboratories, Hercules, CA) as previously described (Finnell ...
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bioRxiv - Cell Biology 2022Quote: The GST-FERRY interactor screen was performed at 4 °C in gravity flow filter columns (Poly- Prep® Chromatography Column, Bio-Rad). 500 μl Glutathione Sepharose 4B (GE Healthcare ...
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DNA Recognition and Induced Genome Modification by a Hydroxymethyl-γ Tail-Clamp Peptide Nucleic AcidbioRxiv - Genetics 2023Quote: ... Droplets were allowed to rest at 4 °C for at least 30 min after cycling and were then read using the QX200 ™ Droplet Reader (Bio-Rad). Data were analyzed using QuantaSoft™ software ...
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bioRxiv - Cancer Biology 2023Quote: ... the slides were stained in different cycles overnight at 4°C with the following antibodies: anti-CD3 (clone CD3-12; #MCA1477, Bio-Rad, USA), anti-CD206 (clone E6T5J ...
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bioRxiv - Molecular Biology 2023Quote: ... the sample boiled for five minutes at 95 °C and size-separated by SDS-PAGE using a 4–20% Mini-PROTEAN® TGX™ Gel (Biorad). Proteins were transferred onto a nitrocellulose membrane (Biorad ...
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bioRxiv - Biochemistry 2022Quote: ... adjusted to equal concentration for each sample and boiled at 95°C for 10 minutes in Laemmli buffer (Bio-Rad #161-0747).
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated to 50°C for 2 to 3 min before the addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexed vigorously for 5 sec before pipetting in plug mould (Bio-Rad 1703713) ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated for 1-2 h at 4 °C and incubated overnight with pre-washed bio-beads (Bio-Rad Laboratories) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... alum pellets were resuspended in sample loading buffer and boiled at 95 °C for ten minutes before being applied to a precast polyacrylamide gel (4–20% Mini-PROTEAN®, Bio-Rad). Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were washed 3 times with ice-cold PBS and boiled at 90 °C in 2x Laemnli sample buffer (Bio-Rad 1610737) for 5 minutes the following day for elution.
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bioRxiv - Biophysics 2023Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Genetics 2023Quote: ... and 0.2 μl of 10X NEB 2.1 buffer were mixed gently in a PCR tube and incubated at 25°C for 15 minutes in a Thermal Cycler (Bio-Rad T100) and then 1 μL ssDNA repair template [IDT] and Phenol red (0.3 μL ...
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bioRxiv - Cancer Biology 2023Quote: Pellets of 1×106 treated cells were incorporated in 1% low melting agarose at 50°C and cast into molds (Biorad, Grabels, France) avoiding air bubbles ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Genomics 2021Quote: ... The proteins were separated in the first dimension on 4-7 and 5-8 IPG strips (Bio-Rad). The strips were then loaded onto 8-20% gradient PAGE for separation on the second dimension ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were prepared to a final concentration of 5 μg/μl in 4x Laemmli buffer (Bio-Rad) containing 25 mM DTT ...
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bioRxiv - Physiology 2021Quote: ... Relative NKA and NHE protein abundance (n = 5 per treatment) was quantified using the Image Lab software (version 6.0.1; BioRad) and normalized by the protein content in each lane.
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bioRxiv - Microbiology 2020Quote: ... Soluble protein suspension was loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (5 mL; Bio-Rad Laboratories) connected to the NGC Scout Chromatography system (Bio-Rad Laboratories) ...
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bioRxiv - Genetics 2020Quote: ... The protein was pulled down by a disposable column loaded with 5 ml Nuvia IMAC resin (BioRad Lab) at 1 ml min-1 ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein extracts were loaded and run on 5-20% gradient pre-cast gels (Bio-Rad) before being transferred onto a 0.45 μM nitrocellulose membrane (Amersham ...
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bioRxiv - Neuroscience 2023Quote: ... with 5% β-Mercaptoethanol then loaded on 4-20% Criterion TGX Precast Midi protein gels (Bio-Rad #5671094). The gels were run in TGS buffer (1x ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: 10 μg of protein samples harvested from xenografted human cells were separated in pre-casted 4-15% gradient SDS gels (Biorad, #456-1084) and transferred to nitrocellulose membranes (Millipore #IPVH00010) ...