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Citations for Bio-Rad :
801 - 850 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation at 10,000xg for 10 minutes at 4°C and then boiled in 2x Laemmli Sample Buffer (Bio-Rad). After SDS-PAGE ...
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bioRxiv - Developmental Biology 2021Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixture was incubated for 30 minutes at 30°C and stopped by adding 12 μl of Laemmli sample buffer (Biorad). After HMT reactions ...
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bioRxiv - Microbiology 2021Quote: ... for 10 minutes at 37 °C and analyzed with Bio-Rad S3 Cell Sorter (Bio-Rad Laboratories Inc., California, USA) and ProSort™ Software using green fluorescence as a trigger.
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bioRxiv - Immunology 2021Quote: Steady-state equilibrium binding of hCoV-2IG lots was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant SARS-CoV-2 RBD proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
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bioRxiv - Cell Biology 2021Quote: ... The membrane was subsequently washed 3 times in TBS+0.05 % Tween-20 over 45 min and incubated with secondary antibodies for 1 h at RT (20 °C) after washing 3 times in TBS+0.05 %Tween-20 and developed by enhanced chemiluminescence (Bio-Rad) with Image Quant™ LAS 4000.
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bioRxiv - Microbiology 2022Quote: ... as counterstain to denote stained areas and incubated at 37°C in a humidified chamber according to the manufacturer’s instructions (Bio-Rad kit 357-2112 ...
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bioRxiv - Cell Biology 2022Quote: ... Electrophoresis was performed for 21 h at 14°C in 0.9% (wt/vol) Pulse Field Certified Agarose (Bio-Rad Laboratories) in exactly 2.4L of 0.5x Tris-borate/EDTA buffer in a Rotaphor apparatus (Biometra ...
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bioRxiv - Molecular Biology 2022Quote: ... The mixtures were incubated for 20 min at 37°C and loaded onto a Bio-Dot Microfiltration Apparatus (Bio-Rad) loaded with nitrocellulose (0.2 μm Amersham Protran ...
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bioRxiv - Immunology 2022Quote: ... and read fluorescence for 45 seconds at 60°C using the CFX384 Touch Real-Time PCR Detection System (Bio-Rad). The expression levels for each gene were then calculated per the 2-ΔΔCT equation [25].
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bioRxiv - Molecular Biology 2023Quote: ... PCR was achieved in 12 cycles and at optimized melting temperature (tm) of 68.2 °C thorough C1000 touch thermal cycler (Bio-rad). For single stranded PCR product (ssPCRP ...
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bioRxiv - Synthetic Biology 2022Quote: ... All reagents except the double-stranded beacon were first assembled in a total volume of 9 μL and run at 37 °C in a CFX96 real-time PCR detection system (Biorad). Both WT SpCas9 and SpCas9-NG are highly active at this temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... The reaction mixtures were held at 10°C until the PCR tubes were removed from the thermocycler (C1000 Touch, BioRad). After ...
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bioRxiv - Molecular Biology 2022Quote: ... The plates were incubated at 30°C and pictures were taken with the setting “Blots: Colorimetric” using a ChemiDoc (BioRAD).
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bioRxiv - Cell Biology 2022Quote: ... PIK3CA c.1633G>A (p.E545K) and PIK3CA c.1624G>A (p.E542K) point mutations were performed on the QX200 ddPCR system (Bio-Rad Laboratories) by using PrimePCR ddPCR Mutation assays according to manufacturer’s instructions (Bio-Rad Laboratories) ...
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bioRxiv - Developmental Biology 2022Quote: ... Protein lysates in Laemmli buffer were separated by electrophoresis on 12% SDS-PAGE gels and transferred for 2 hours at 4°C on to a PVDF membrane (BioRad). After blocking for an hour in 5% dry milk ...
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bioRxiv - Molecular Biology 2023Quote: ... plates were incubated for 4d at 30°C and photographed before washing using the ChemiDoc XRS+ molecular imager (Bio-Rad) under the blot/chemicoloric setting with no filter ...
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bioRxiv - Synthetic Biology 2023Quote: ... The reaction was kept at 37°C and recorded using a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). To prevent evaporation the plate was sealed with a transparent sticker ...
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bioRxiv - Molecular Biology 2023Quote: ... Binding was performed with gentle mixing for 1 hour at 4°C and loaded onto a Poly Prep Chromatography Column (BioRad). The loaded resin was then washed 3-4 times with 6 ml Wash Buffer (50 mM sodium phosphate ...
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bioRxiv - Microbiology 2023Quote: ... and 0.2M DTT) for 10 minutes at 95°C before loading in a 10% SDS-PAGE Gel (Bio-Rad 4561034). Sample proteins were separated in 1X SDS Running Buffer (25mM Tris ...
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bioRxiv - Molecular Biology 2023Quote: ... These mixtures were then heated up to 95 °C for 5 minutes and slowly cooled to room temperature at the rate of 1 °C per minute using a thermal cycler (BioRad).
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bioRxiv - Immunology 2023Quote: ... Protein denaturation before separation was done by treating the cell lysates for 3 min at 95°C in presence of 1x XT Sample Buffer (BioRad) and 1x XT reducing agent (BioRad) ...
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bioRxiv - Biochemistry 2022Quote: ... The PVDF membranes were then incubated with HRP-coupled secondary antibodies (1:10,000, CST) for 2 h at 25°C and visualized by ChemiDoc XRS (Bio-Rad) instrument.
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bioRxiv - Microbiology 2022Quote: Real-time-PCR was performed with Maxima SYBR Green qPCR Master Mix (Applied Biosystem, #4309155) at an annealing temperature of 60°C on a CFX96 touch Real-Time PCR machine (Biorad) using the following primers:
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bioRxiv - Cancer Biology 2023Quote: ... held at 4 °C) was performed in a C1000 Touch Thermal Cycler with 96-Deep Well Reaction Module (Bio-Rad). After RT reaction ...
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bioRxiv - Genetics 2023Quote: ... DNA was resolved by electrophoresis in a 0.7% agarose gel which was dried for two hours at 50°C using 583 gel dryer model from Bio-Rad. Dried gel was hybridized in Church-Gilbert solution (1% BSA ...
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bioRxiv - Biochemistry 2023Quote: ... Blue Native PAGE gel experiments were performed in a cold room (4 °C) using 10% precast gels (BioRad Mini-PROTEAN) and run at 170 V for 55 minutes ...
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bioRxiv - Systems Biology 2023Quote: ... and heated at 95°C for 60 minutes in a thermal cycler (Bio-Rad S1000 with 384-well reaction module) at a constant lid temperature of 110°C ...
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bioRxiv - Bioengineering 2023Quote: ... 200 ng of purified RNA was immediately reverse transcribed at 37 °C for one hour in an S1000 Thermal Cycler (BioRad) via the OmniScript RT Kit (Qiagen ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were stained with primary antibody in blocking buffer at 4°C overnight: anti-NP (1:5000, OBT1555, Bio-Rad), anti-PA ...
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bioRxiv - Neuroscience 2023Quote: ... using pre-treated (95°C for 10 min to reduce adherence) PCR tubes and optical flat caps (Bio-rad, Belgium). The final reaction volume was 20 µL (10 µM of miniMeCP2 or 20 µM of miniMeCP2T158M) ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples were boiled 5min at 90°C and 10µl was loaded on a 4-20% gradient SDS-PAGE gel (Biorad #4561096). Gel was stained with Coomassie brilliant blue R.
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bioRxiv - Microbiology 2023Quote: Protein samples were centrifuged (150,000 × g for 10 min at 4°C) and loaded onto an Enrich SEC 650 10 × 300 column (Bio-Rad) using the AKTA explore system (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... the plates were developed at 100 °C for 20 min and further analyzed with the ChemiDoc-System (Bio-Rad, USA).
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bioRxiv - Immunology 2024Quote: 96-well flat-bottom NUNC Maxisorp plates were coated overnight at 4 °C with 0.5 mg/mL recombinant HBsAg (BIO-RAD). Plates were washed with PBS/T ...
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bioRxiv - Bioengineering 2023Quote: ... and the supernatant was incubated at 95°C for 10 minutes in the presence of Laemmli sample buffer (Bio-Rad). Proteins (20 μg per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... DNA was then eluted and reverse-crosslinked by incubating the beads at 95°C for 10 min in presence of 100 µl of 10 % Chelex resin (BioRad), treated with Proteinase K (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... 95 °C) and 1.25 µg sample was resolved on a 4-12% acrylamide Criterion XT Bis-Tris gel (Bio-Rad) in MOPS buffer at 100 V for 20 min and subsequently at 150V for 190 min on ice along with PageRuler™ Plus Prestained Protein Ladder (Thermo Scientific ...
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bioRxiv - Microbiology 2024Quote: ... the samples were boiled at 95°C for 10 minutes and resolved on a TGX stain-free gel (Bio-Rad) for SDS-PAGE analysis ...
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bioRxiv - Molecular Biology 2024Quote: ... and heat denatured at 95°C for 10 min before loading onto a 4-15% Tris-glycine gel (Bio-Rad) and electrophoresed at 125 V ...
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bioRxiv - Microbiology 2023Quote: ... cells and plasmids in 10% glycerol were first heat-shocked at 56°C for 2 min followed by immediate electroporation in 0.1-cm cuvette (Bio-Rad) using electroporation program of 2.5 kV and time constant of 2.3-2.5 ms on the Bio-Rad Micropulser ...
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bioRxiv - Bioengineering 2024Quote: ... Samples in 8-well tubes were heat-shocked for three minutes at 72 °C (C1000 Touch Thermal Cycler, Bio-Rad), placed on wet ice for two minutes ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were heated from 35 °C to 79°C at a rate of 0.3 °C per 15 s in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad). Fluorescence intensity was determined using excitation (λem=525/20 nm ...
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bioRxiv - Cell Biology 2024Quote: ... eluate was incubated at 70 °C then run on 4-12% NuPAGE gell and transferred to LF PVDF membrane (BioRad). Western-blot analysis were done with the next antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Membranes were blocked with 5% non-fat dry milk and incubated with primary antibodies overnight at 4 °C and then incubated with host species-matched HRP-conjugated secondary antibodies (BioRad) for 1 hour at room temperature ...
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bioRxiv - Genetics 2024Quote: ... chromosomal DNA was prepared in 1.3% of low-melting point agarose plugs as described in 74 and run at 14 °C with 1X TBE buffer in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120°angle for 48 h ...
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bioRxiv - Biochemistry 2024Quote: ... denatured for 10 min at 96°C before it was separated at a 4-20 % PROTEAN TGX stain-free gel (BioRad) at 200 V for 30 min ...
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bioRxiv - Plant Biology 2023Quote: ... Linearized plasmid was mixed with 250 μL of cells at 15°C in a 0.4 cm gap electroporation cuvette and transformed immediately by electroporation using a Gene Pulser II (Bio-Rad) set to 800V and 25 μF ...
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bioRxiv - Immunology 2021Quote: Concentration of 27 inflammation-related human proteins were measured in cell culture supernatants by using Human 27-plex BIORAD cytokine panel (Cat. No.171-A1112, BIORAD). Standard curves were generated for all 27 cytokine standards with eight 2-fold serial dilutions starting from 32 ng/ml ...
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bioRxiv - Cell Biology 2021Quote: ... except that blocking was done with phosphate-buffered saline plus 0.1% Triton X-100 (PBS-T) containing 5% nonfat dry milk (Biorad Blotting grade Blocker) and 10 mM NaN3 ...