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Citations for Bio-Rad :
1101 - 1150 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Samples were heated to 50°C for 2 to 3 min before the addition of 40 μl molten CleanCut agarose (Bio-Rad 1703594), vortexed vigorously for 5 sec before pipetting in plug mould (Bio-Rad 1703713) ...
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated for 1-2 h at 4 °C and incubated overnight with pre-washed bio-beads (Bio-Rad Laboratories) at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... alum pellets were resuspended in sample loading buffer and boiled at 95 °C for ten minutes before being applied to a precast polyacrylamide gel (4–20% Mini-PROTEAN®, Bio-Rad). Proteins were then transferred to a nitrocellulose membrane using the Trans-Blot Turbo transfer system (Bio-Rad) ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Microbiology 2024Quote: ... The samples were run on a low constant voltage at 4°C for 6 to 8 h in Tris Tricine SDS Running Buffer (Bio-Rad, USA). The peptides were detected by Instant Blue (Expedeon Inc.) ...
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bioRxiv - Microbiology 2024Quote: ... The samples were run on a low constant voltage at 4°C for 6 to 8 h in Tris Tricine SDS Running Buffer (Bio-Rad, USA). The peptides were detected by Instant Blue (Expedeon Inc.) ...
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bioRxiv - Biophysics 2024Quote: ... This mixture was then incubated at 4 °C for 1 hour and further subjected to a 4-hour incubation with bio-beads (Bio-Rad, USA). After incubation ...
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bioRxiv - Immunology 2023Quote: ... Serially diluted recombinant human IgG1 or human IgG4 (BioRad, Hercules, CA) with unrelated specificities were used for quantification in separate wells on the same plate.
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bioRxiv - Genomics 2021Quote: ... The proteins were separated in the first dimension on 4-7 and 5-8 IPG strips (Bio-Rad). The strips were then loaded onto 8-20% gradient PAGE for separation on the second dimension ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were prepared to a final concentration of 5 μg/μl in 4x Laemmli buffer (Bio-Rad) containing 25 mM DTT ...
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bioRxiv - Physiology 2021Quote: ... Relative NKA and NHE protein abundance (n = 5 per treatment) was quantified using the Image Lab software (version 6.0.1; BioRad) and normalized by the protein content in each lane.
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bioRxiv - Microbiology 2020Quote: ... Soluble protein suspension was loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (5 mL; Bio-Rad Laboratories) connected to the NGC Scout Chromatography system (Bio-Rad Laboratories) ...
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bioRxiv - Genetics 2020Quote: ... The protein was pulled down by a disposable column loaded with 5 ml Nuvia IMAC resin (BioRad Lab) at 1 ml min-1 ...
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bioRxiv - Cell Biology 2022Quote: ... Total protein (5-10 ug) was loaded in 15 well pre-casted gradient gel (456-8086, Bio-Rad). After electrophoresis ...
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bioRxiv - Cell Biology 2023Quote: ... 20 μg of protein extracts were loaded and run on 5-20% gradient pre-cast gels (Bio-Rad) before being transferred onto a 0.45 μM nitrocellulose membrane (Amersham ...
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bioRxiv - Neuroscience 2023Quote: ... with 5% β-Mercaptoethanol then loaded on 4-20% Criterion TGX Precast Midi protein gels (Bio-Rad #5671094). The gels were run in TGS buffer (1x ...
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bioRxiv - Synthetic Biology 2024Quote: ... was added into (Ribosome purification: 5 mL; PURE proteins purification: 2-3 mL) Econo-Pac chromatography columns (Biorad). The column was washed with 50 mL deionized Millipore water and charged with 15 mL 0.1 N Nickel sulphate solution ...
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bioRxiv - Immunology 2024Quote: Samples were prepared in 1 x Laemmli buffer with or without 1 x dithiothreitol (Biorad). Samples were then heated for 10 min at 95 °C and loaded onto a precast NuPAGE™ 4-12 % Bis-Tris polyacrylamide gel in NuPAGE™ MES SDS running buffer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2020Quote: 10 μg of protein samples harvested from xenografted human cells were separated in pre-casted 4-15% gradient SDS gels (Biorad, #456-1084) and transferred to nitrocellulose membranes (Millipore #IPVH00010) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with fresh serum-free medium containing 10% CCK-8 for 1 hour at 37°C and then the absorbances at 450 nm were measured using a microplate reader (Bio-Rad, Hercules, USA).
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bioRxiv - Cell Biology 2020Quote: ... followed by 1◻h incubation at 4◻°C with HRP-conjugated goat anti-mouse (1:5000 dilution in 1% YE in PBS-T, BioRad #170–6516) secondary antibody ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatants were collected and stored at −80°C and cells were lysed in 1x Laemmli Buffer (Bio-Rad Laboratories, Inc., Hercules, CA, USA) for further analysis.
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bioRxiv - Neuroscience 2021Quote: ... and heated at 100 °C for 10 min before loading into 12% Mini-PROTEAN TGX Stain-Free gels (Bio-Rad Laboratories, Hercules, CA). Separated proteins were transferred onto nitrocellulose membranes (Bio-Rad Laboratories ...
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bioRxiv - Bioengineering 2021Quote: ... The obtained DNA extract (100 μL) was stored at −20 °C until further investigation and subjected to qPCR analysis using 96-well plates (Bio-Rad, Hercules, USA), the Thermocycler CFX connect system (Bio-Rad ...
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bioRxiv - Neuroscience 2019Quote: ... were heated at 99 °C for 3 minutes and subjected to 4-20% gradient SDS-PAGE prior to transfer to nitrocellulose membranes (Bio-Rad Laboratories, Inc.). Membranes were then blocked for 1 hour with 5% (w/v ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were recovered from the resulting BAL fluid by centrifugation (500 g, 20 min, 4°C) and counted using an automated cell counter (TC20; Bio-Rad, Hercules, CA). All animal care procedures and experiments were approved by the University of Arizona Animal Care and Use Committee (Approval #13-490).
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bioRxiv - Plant Biology 2021Quote: ... Quantitative real-time PCR (RT-qPCR) analysis was carried out using a CFX384 Real-Time System (C 1000 Touch Thermal Cycler, Bio-Rad, Hercules, California) using SensiFAST SYBR No-ROS kit (Bioline ...
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bioRxiv - Microbiology 2020Quote: Protein samples were separated on 12% gels and then transferred to nitrocellulose membranes (Hybond-C; Amersham Life Sciences, UK) using a semi-dry transfer apparatus (Bio-Rad Laboratories, USA). The membranes were blocked with 5% (w/v ...
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bioRxiv - Biophysics 2020Quote: ... placed on a heat block (+90 °C) for 10 min and sonicated before separation by SDS-PAGE (4-20% Mini-PROTEAN TGX Gels, Bio-Rad, 456-1096). Next ...
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bioRxiv - Cell Biology 2022Quote: ... in 4°C for 2-4 hours and the beads were then isolated and eluted through a Bio-Spin® Chromatography Column (Bio-Rad, #7326008) with elution buffer (5 0mM Tris-HCl pH 8 ...
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bioRxiv - Immunology 2020Quote: ... Size fractioned FBS-free medium from BNT162b1 RNA-transfected HEK293T cells was analysed by denaturing (95° C) and non-denaturating (no-heating) PAGE using 4–15% Criterion™ TGX Stain-Free™ Gel (Bio-Rad) and western blot ...
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bioRxiv - Molecular Biology 2019Quote: ... The lysate/bead mixtures were incubated at 4°C on a rotating platform for 2 hrs and subsequently poured into Poly-Prep® Chromatography Columns (Bio-Rad #7311550). Unbound fractions were collected through the columns and stored at −20°C ...
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bioRxiv - Molecular Biology 2020Quote: ... RPA incubation was done at 39 °C for 25 min with no agitation and using a PTC-0200 DNA Engine Cycler (BIO-RAD, United States) with no lid heating ...
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bioRxiv - Synthetic Biology 2019Quote: ... Plates were incubated overnight at 37°C then visualized by imaging with a Chemi-Doc camera and QuantityOne imaging software (Bio-Rad, Hercules, CA). E.coli containing pUC19 ...
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bioRxiv - Cell Biology 2020Quote: ... fixed in 4% paraformaldehyde for 2 h at 4 °C and embedded in PBS containing 3% low-melting point agarose (Bio-Rad, California, USA). The agarose gels containing the fixed organoids were processed in a standard automated tissue histology processor ...
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Gatekeeper helix activates Golgi SM protein Sly1 and directly mediates close-range vesicle tetheringbioRxiv - Cell Biology 2020Quote: ... The resulting mixtures were dialyzed (20 kDa cutoff) for ∼18h at 4°C in the dark against 250 volumes of FB160M1 containing 2 g BioBeads SM2 (Bio-Rad, Hercules, CA) per 2 mL of RPL mixture ...
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bioRxiv - Molecular Biology 2019Quote: ... The samples were boiled (95°C) and 18 µL were loaded onto a 4-20% Mini-PROTEAN®TGX™ 12 well-gel (Biorad®). After transfer onto a PVDF (Amersham Hybond ...
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bioRxiv - Bioengineering 2020Quote: ... Membranes were incubated with primary antibodies overnight at 4°C and for 1 h at room temperature (RT) with secondary horseradish peroxidase conjugated antibodies (Bio-Rad, 1:3000). Membranes were then developed using the Clarity Western ECL Substrate (Bio-Rad ...
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bioRxiv - Plant Biology 2020Quote: ... were centrifuged at 5000 x g for 15 min at 4°C and resolved by SDS-PAGE prior to transfer to a PVDF membrane (BioRad, Hercules, CA, USA). The membrane was then incubated with SnTox3 primary antibodies ...
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bioRxiv - Physiology 2021Quote: ... The solution with MSP1E3D1 was mixed at 4°C for 10 min before addition of 130 mg of Biobeads SM2 (Bio-Rad, Hercules, CA). Biobeads (washed into methanol ...
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bioRxiv - Biochemistry 2020Quote: Surface biotinylation samples were heated to 50°C for 10 minutes before separation using 4-20% polyacrylamide gradient gels (Bio-Rad, Hercules, CA). After separation ...
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bioRxiv - Neuroscience 2019Quote: ... RNA probes were then alkaline hydrolyzed (80mM NaHCO3, 120mM Na2CO3) for 60°C for 15 minutes and purified using a microcolumn (Bio-Rad 732-6223). Probe integrity was assessed by agarose gel and kept at −80°C when not in use.
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bioRxiv - Bioengineering 2021Quote: ... samples of 5-10 μg protein were reduced with 50 mM dithiothreitol (DTT) for 15 minutes at 90°C and mixed with 4× sample loading buffer (Bio-Rad, Munich, Germany). Protein separation was carried out via SDS-PAGE in 12% polyacrylamide gels (Mini-PROTEAN® TGX™ Precast Gels ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated briefly at 90°C and then transferred to a Trans-Blot Turbo mini nitrocellulose membrane using Trans-Blot Turbo Transfer system (Bio-Rad, Hercules, CA). The membrane was incubated overnight in antibody buffer (50mM tris-HCl ...
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bioRxiv - Molecular Biology 2020Quote: ... Sample DNA was digested at 37°C for 15 minutes and loaded in ddPCR DG8™ Cartridge with QX200™ droplet generation oil (Bio-Rad). Droplets were made using QX200™ droplet generator (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... The plates were incubated at 37°C for different time points (0,4hr,12hr,24hr,72hr) and was measured on an ELISA reader (Bio-Rad, Hercules, CA, USA) at a wavelength of 570 nm and O.D was recorded.
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bioRxiv - Biochemistry 2022Quote: REST-FL was purified in two-step chromatography and carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2022Quote: ... carried out in a cold room at 4°C with a chromatography system (NGC™ Chromatography System Quest™ 10 Plus, Bio-Rad).
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bioRxiv - Biochemistry 2024Quote: ... The fluorescence signal was measured at 30 s intervals at 37°C using a CFX96 touch real-time PCR system (Bio-Rad, CA, USA).
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bioRxiv - Microbiology 2024Quote: ... Samples were heated for 10 min at 70°C and then loaded on a 12% Mini-protean TGX gel (Bio-Rad Cat# 4561044). The gels were run for approximately 1 h at 100 V ...