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Citations for Bio-Rad :
751 - 800 of 10000+ citations for Human Chemokine C X C Motif Receptor 5 CXCR5 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Triton-insoluble material was then pelleted at 15890 x g for 10 min and protein concentration measured with the DC assay (Bio-rad). For immunoblot analysis ...
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bioRxiv - Immunology 2022Quote: ... was centrifuged at 500 x g for 10 min Protein levels in the supernatant were measured by Bradford assay (Bio-Rad), and cytokine levels were measured using a custom multiplex ELISA (ThermoFisher Scientific).
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bioRxiv - Evolutionary Biology 2019Quote: ASM and AIM proteins were dissolved in 2X Laemmli buffer (95% buffer - 5% beta-mercaptoethanol) (BioRad). As observed by Ramos-Silva et al ...
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bioRxiv - Microbiology 2019Quote: ... Ten microliters of each sample and 5 μL of Precision Plus Protein Dual Xtra standard (BioRad) were loaded onto a 4-20% TGX precast gel (BioRad ...
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bioRxiv - Microbiology 2019Quote: ... Protein was applied at 1 ml/min to a 5 ml ceramic hydroxyapatite column (BioRad BioscaleTM) in the dialysis buffer ...
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bioRxiv - Microbiology 2021Quote: ... Protein was applied at 1 ml/min to a 5 ml ceramic hydroxyapatite column (BioRad BioscaleTM) in the dialysis buffer ...
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bioRxiv - Biochemistry 2022Quote: ... Protein lysate (40 mg per lane) was separated by SDS-PAGE (5-20% gradient, Bio-rad) at 125V (constant voltage ...
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bioRxiv - Cell Biology 2023Quote: ... Equal aliquots of protein were loaded onto 5-20% polyacrylamide gels (Bio-Rad, Hercules, CA, USA) run for 80 minutes at 50V then blotted onto PVDF membrane (Millipore ...
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bioRxiv - Genetics 2021Quote: ... unspecific binding sites were blocked over night at 4°C with 3% milk powder (Marvel) in Tris Buffered Saline solution (BioRad) including 1% Tween 20 (Sigma ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were cleared by centrifugation at 14,000×g for 30 min at 4°C and equalized using quick start Bradford 1x dye reagent (BioRad, 5000205). Ni-NTA resin was washed three times with lysis buffer containing 20mM imidazole pH 8.0 and 5mM β-mercaptoethanol while samples were prepared ...
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bioRxiv - Cell Biology 2022Quote: ... using the BioRad transfer system at 100 V for 60 min at 4°C or with Trans-Blot Turbo Mini PVDF Transfer Packs using Trans-Blot Turbo Transfer System (Biorad). The membrane was blocked for 30 min with the Odyssey Blocking Buffer (TBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD). For liquid cultures cells were diluted to OD600 0.15 and incubated at 30°C ...
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bioRxiv - Plant Biology 2019Quote: Proteins were separated on denaturing SDS-PAGE followed by an electrotransfer at 4 °C onto a PVDF membrane (0.2 µM, Bio-Rad). NRT2.1 was detected using three different anti-NRT2.1 antisera produced by Eurogentec against either the synthetic peptide TLEKAGEVAKDKFGK (anti-NRT2.1 19) ...
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bioRxiv - Cancer Biology 2019Quote: ... and extension at 72°C for 30 seconds using the CFX96 Real-Time PCR Detection System (Bio-Rad Laboratories, Inc.). Analysis and fold differences were determined using the comparative threshold cycle method ...
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bioRxiv - Microbiology 2019Quote: ... boiled (95°C, 10 min), centrifuged (1,000 × g, 2 min) and loaded on 10% or 4-20% SDS-PAGE gel (Bio-Rad). Following electrophoresis (60 min ...
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bioRxiv - Molecular Biology 2019Quote: ... Samples were boiled at 95 °C for 10 mins with 6×SDS sample buffer before loading onto 4–20% Tris-glycine gels (BioRad). Resolved proteins were transferred to nitrocellulose membranes using the Trans-Blot® Turbo system (BioRad ...
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bioRxiv - Developmental Biology 2019Quote: ... monomeric ATP-bound 2 μM rabbit muscle actin was treated overnight at 4°C with analytical grade anion exchange resin (BioRad), hexokinase (Sigma-Aldrich) ...
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bioRxiv - Cancer Biology 2019Quote: ... The eluted DNA and 10% of respective input DNA were reverse cross-linked at 65°C overnight and used for the qPCR using SYBR Green qPCR mix and a CFX96 instrument (BioRad).
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bioRxiv - Genetics 2021Quote: ... temperature of hybridization 61°C) and fluorescent intensity was measured in a QX100 Droplet Reader (Bio-Rad Laboratories, Hercules, CA) and the signal data was analysed using QuantaSoft Analysis Pro (1.0.596 ...
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bioRxiv - Genetics 2021Quote: ... rotated at 4°C for 2h and then poured into a Econo-Column® Chromatography column (Bio-Rad, Hercules, CA). After extensive washing first with 80 mL of 20 mM Tris HCl [pH8@4°C] ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated at 30 °C and pictures were taken every day with the setting “Blots: Colorimetric” using a ChemiDoc (BioRAD).
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bioRxiv - Genetics 2020Quote: ... The reaction was incubated at 37°C for 15 min and stopped by the addition of 10 μl of TBE-UREA-sample buffer (BioRad). The samples were analysed on a 15% denaturing polyacrylamide gel in 1 × TBE buffer.
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bioRxiv - Molecular Biology 2020Quote: ... 95°C), and run (loaded in 2 wells, 20 μL per well) on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Molecular Biology 2020Quote: ... 2.5 mg/ml Orange G), denatured (2 min, 80°C) and run on a 10 well 10% TBE-urea gel (Biorad) (200 V ...
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bioRxiv - Bioengineering 2021Quote: ... The cell pellet was re-suspended in 10mL of fresh culture media (37°C) and cells density was calculated with a TC20 automated cell counter (BioRad). 100’000 to 150’000 cells were plated per 35mm glass-bottom dish and transfected the following day ...
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bioRxiv - Bioengineering 2020Quote: ... NJ) overnight at 4°C and visualized using the Bio-Rad ChemiDoc MP Imaging System (Bio-Rad Laboratories, Hercules, CA).
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bioRxiv - Biochemistry 2021Quote: ... pH 5.3 (murine P-domains) or pH 7.3 (GII.4 Saga P-domain) at 4 °C via Micro Bio-Spin 6 columns (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... Reactions were incubated for 15 min at 30 °C and then applied to a Bio-Gel A-1.5m (Bio-Rad) resin packed in a 0.7×15cm Flex-Column (Kimble) ...
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bioRxiv - Neuroscience 2021Quote: MV pellets were sonicated three times for 10 s at 20 Hz (Vibra cell VCX130) in 2% SDS and heated at 56°C in Laemmli loading buffer (Biorad). The protein content was measured using the Pierce 660 nm protein assay kit (Thermo Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... Extracts were stored at -20°C or immediately resolved on an 7.5% SDS-PAGE pre-cast gel (Bio-Rad, #4568023) and transferred onto a 0.2 μm nitrocellulose membrane (GE healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... with or without a C-terminal FLAG™-tag followed by a foldon trimerization domain and an octahistidine tag was heated to 95 °C in XT sample buffer (Biorad) for 5 minutes ...
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bioRxiv - Microbiology 2020Quote: Steady-state equilibrium binding of post-vaccination rabbit polyclonal serum was monitored at 25°C using a ProteOn surface plasmon resonance (BioRad). The purified recombinant Spike proteins were captured to a Ni-NTA sensor chip with 200 resonance units (RU ...
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bioRxiv - Neuroscience 2020Quote: ... The mixtures were incubated at 37°C in micro PCR tubes on a thermal cycler (BioRad DNA Engine PTC-200). At the indicated time points ...
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bioRxiv - Biophysics 2020Quote: ... before being transferred to Whatman 3 MM paper and dried (50°C, 2 hours) using a gel dryer (Model 583, BioRad) attached to a vacuum pump ...
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bioRxiv - Developmental Biology 2022Quote: Cells in monolayer were fixed in 4% PFA for 20 minutes at 4°C and blocked for 30 minutes in 10% donkey serum (BioRad) and 0,1% Triton X-100 (Sigma-Aldrich) ...
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bioRxiv - Microbiology 2022Quote: ... centrifuged for 20 minutes at 4°C and supernatants mixed 3:1 with 4x Laemmli sample buffer (Bio-rad 1610747). Samples were heated at 95°C for 5 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... boiled at 99 °C for 10 min and supernatants were loaded on 4–12% Criterion XT Bis–Tris precast gel (Biorad). The gel was fixed with 40% Methanol and 10% acetic acid and then stained for 1 hour using colloidal coomassie dye G-250 (Gel Code Blue Stain ...
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bioRxiv - Molecular Biology 2019Quote: ... Plates were incubated overnight at 30 °C and imaged the following day using a Gel Doc EZ Gel Documentation System (BioRad) and Image Lab (BioRad ...
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bioRxiv - Microbiology 2020Quote: ... Real-time PCR was performed using SYBR Green (Applied Biosystem – 4309155) and primers listed in Table S1 at an annealing temperature of 60°C on a CFX96 thermal cycler (Biorad). Generated data were analysed using the CFX Manager Software (Biorad).
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bioRxiv - Microbiology 2020Quote: ... it was activated in an oven at 120°C and imaged using a gel doc XR image system (Bio-Rad).
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bioRxiv - Biophysics 2019Quote: ... Reactions were incubated at 30°C for 60 minutes and then analyzed by denaturing TBE-Urea PAGE with fluorescence detection (ChemiDoc MP, BioRad).
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bioRxiv - Microbiology 2019Quote: ... The drips were allowed to dry and the plates were incubated at 37°C for 16 h and imaged using the fluorescein setting on a ChemiDoc MP Imaging machine (Biorad).
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bioRxiv - Microbiology 2020Quote: ... The samples were heated at 90°C for 10 minutes and loaded onto a 4-20% precast polyacrylamide gel (BioRad). The gel was stained with SimplyBlue SafeStain (Invitrogen ...
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bioRxiv - Genomics 2019Quote: ... at 4 °C and transferred to a nitrocellulose membrane (0.45 μM) by semidry transfer (BioRad Trans-Blot SD transfer cell) at a constant current of 0.12 A for 1 □ hour ...
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bioRxiv - Microbiology 2021Quote: ... for 60 min at 37°C followed by purification on a Micro-Bio Spin P-6 Gel Column (Bio-Rad). 1 pmol of 5’-end-radiolabeled primer was used for each extension reaction ...
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bioRxiv - Genetics 2019Quote: ... chromosomal DNA was prepared in agarose plugs as described in (Bani Ismail et al. 2014) and run at 14 °C in a CHEF DR-III apparatus (BioRad) using the field 6V/cm at a 120° angle ...
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bioRxiv - Molecular Biology 2019Quote: ... containing 2μL of 20X reducing buffer at 95°C for 10 min and separated by SDS-PAGE short migration on 12% SDS-PAGE (Criterion XT Precast gels–BioRad). After a short migration ...
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bioRxiv - Microbiology 2019Quote: ... Samples were boiled for 10 min at 100°C in SDS-PAGE sample buffer and separated on Tris-glycine 4-20% SDS-PAGE gels (BioRad). Detected protein bands for each blot were quantified using ImageJ software for gel densitometry measurements of NSP4:GAPDH.
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bioRxiv - Microbiology 2019Quote: ... The lysate was used to bind to 200ul of IgG Sepharose beads (Amersham Biosciences) for 2h at 4°C using a disposable chromatography column (BioRad). The IgG-Sepharose column was washed with 35 ml of protein A binding buffer (10 mM Tris–HCl ...
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bioRxiv - Cell Biology 2020Quote: ... The embryos were mounted on a 2 % agarose pad and heat-shocked at 30°C for 1h (Thermocycler Bio-Rad). After 2h recovery at 20°C ...