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Citations for Bio-Rad :
9301 - 9350 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... The quality of extracted DNA samples was also checked by loading one μl of DNA in 1% Agarose Gel (Bio-Rad, California, USA). The extracts were stored at -20 C until use.
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bioRxiv - Developmental Biology 2020Quote: ... protein samples were boiled with β-mercaptol and ran in SDS-PAGE gels casted from 30% Acrylamide/Bis solution 29:1 (Bio-rad, 1610156) using the Mini-PROTEAN® system (Bio-rad) ...
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bioRxiv - Genomics 2019Quote: ... the integrity and quantity of total RNA was confirmed by 1% agarose gel electrophoresis and the Experion™ automated electrophoresis system (Biorad Laboratories, Australia).
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bioRxiv - Physiology 2021Quote: ... Consistent protein loading and sufficient transfer of proteins was confirmed using 1× Amido Black stain and images were captured using a Versadoc System (Bio-Rad, Mississauga, ON). Membranes were blocked overnight at 4°C with Tris-buffered saline (TBS)/0.1% Tween 20 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2021Quote: ... and gene expression of MCL-1 in the parental and MCL-1 deleted cells were verified by qPCR performed using the iTaq™ Universal SYBR Green Supermix (BioRad, California, USA), according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... cDNAs were subjected to PCR analyses using specific primers (The primer sequences are listed in Table 1) on an amplification detection system (Bio-Rad, Hercules, USA). β-actin served as a housekeeping gene ...
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bioRxiv - Genetics 2021Quote: ... 1 mM dithiothreitol and 0.5% Tween 20] for 24 hours and its concentration was quantified by Bradford assay (Bio-Rad, Des Plaines, IL).
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bioRxiv - Immunology 2022Quote: ... and 1 µl of respective primer pairs (200 nM final concentration) were mixed and distributed into 96-well qPCR plate (Bio-Rad; cat. #12001925). Droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were boiled 1 min and subjected to SDS-PAGE using a 4-15% Mini-PROTEAN TGX Stain-Free Protein Gel (Bio-Rad Ref. 4568085). After SDS-PAGE ...
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bioRxiv - Cell Biology 2022Quote: 100 microlitres of the secreted fractions and 100 µl of 1:20 diluted (in PBS) lysates from the secretion assays were loaded into a bio-blot microfiltration blotting system (BioRad. Cat. Nº: 1706545). Samples were left to flow through a 0.45 µm nitrocellulose blotting membrane (Amersham Protran ...
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer: 2% Blotting Grade Blocker (Bio-Rad cat. # 1706404) and 2% heat-inactivated goat serum (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... The results were obtained from channels 1 (FAM) and 2 (HEX/VIC) and analysed with QuantaSoft™ Analysis Pro 1.0 (Bio-Rad Laboratories, Inc). All ddPCR reactions were prepared with QX200™ ddPCR™ EvaGreen® Supermix (2x).
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bioRxiv - Immunology 2024Quote: ... cells were lysed in buffer containing 1% NP-40 and proteins were separated using SDS-PAGE (Mini-PROTEAN® TGX™ Precast Gels, Bio-Rad), transferred to low fluorescence PVDF membranes (Bio-Rad) ...
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bioRxiv - Molecular Biology 2024Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) in cell lysate was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Catalogue #: 12004167; Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Immunology 2023Quote: IgE receptor cross-linking (IgECL) on HSMCs was accomplished through sensitization with 1 μg/mL chimeric human IgE anti-NP antibody (MCA333S, BIO-RAD, Hercules, CA) for 24 hours ...
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bioRxiv - Microbiology 2023Quote: The resulting amplicons and their corresponding lengths were visualized and documented via gel electrophoresis and analysis (Quantity 1 software, Bio-rad, CA, USA).
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bioRxiv - Molecular Biology 2023Quote: ... at room temperature for 1 h with slight shaking and SP-A-S protein or SP-A-RBD complexes were detected by adding HRP-conjugated Goat Anti-rabbit IgG (1:2000, Bio-rad, Hercules, USA) for an additional 1 h ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... RNA copy numbers were determined by ddPCR (see AAV virus quantification) using Taqman primers for the WPRE element (Sup. Table 1) and Rpp30 (Biorad, assay ID: dMmuCPE5097025) as housekeeping gene.
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bioRxiv - Immunology 2023Quote: Eluted samples were run for about 1 cm on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (#4569034, Bio-Rad), and the gel was stained with Pierce™ Zinc Reversible Stain Kit (#24582 ...
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ENGRAILED-1 transcription factor has a paracrine neurotrophic activity on adult spinal α-motoneuronsbioRxiv - Neuroscience 2023Quote: ... Gels were run in MOPS buffer at 150 V for 1 hour and 15 min and the proteins transferred for 30 min at 100 V on Millipore PVDF membranes (BIORAD Criterion Blotter apparatus). Membranes were blocked for 1 hour at RT with 4% non-fat dry milk (NFDM ...
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bioRxiv - Plant Biology 2023Quote: ... Mixed samples were immediately centrifuged at 200 x g for 5 mins and 20 μl of the supernatant was transferred into 1 ml Bradford reagent (Bio-Rad, CA, USA) in 1.5 ml tubes ...
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bioRxiv - Cell Biology 2023Quote: ... overnight at 4°C in a humidified chamber with the following primary antibodies: Guinea-pig anti-Insulin antibody (Biorad 5330-0104G, 1:250). Following overnight incubation ...
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bioRxiv - Systems Biology 2023Quote: ... Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Biophysics 2023Quote: ... and 1 mM DTT) to a final KOAc concentration of 75 mM and incubated with hydroxyapatite Bio gel HTP (Bio-Rad #130-0402) for 45 min at 4 °C ...
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bioRxiv - Systems Biology 2023Quote: Proteins were transferred to a nitrocellulose membrane (Carl Roth, 200H.1) using a Trans-Blot® Turbo™ Transfer Starter System (BioRad, 1704150). Membranes were stained with Ponceau S (Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... We incubated the reaction for 1 hour at 50°C in a thermal cycler (Bio-Rad C1000 Touch Thermal Cycler, Bio-Rad, USA).
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bioRxiv - Evolutionary Biology 2024Quote: ... Eggs were subjected to experimental electroporation parameters (voltage = 50 V; pulse = 25 ms; number of pulses = 1) using the Gene Pulser Xcell Eukaryotic System (Bio-Rad, cat. 1652661). Immediately ...
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bioRxiv - Cell Biology 2024Quote: ... 60 μl 1x Laemmli buffer (diluted by mixing same volume of 1 x PBS with 2x Laemmli buffer, Bio-Rad, Catalog number: #1610737EDU) was added to the beads and incubated for 10 min at 70°C ...
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bioRxiv - Immunology 2024Quote: ... The suspended cells were then diluted 1:100 in PBS and counted using a TC20 automated cell counter (Bio-Rad Laboratories, Hercules CA). For the first vaccination with cda1Δ2Δ3Δ (given to CBA/J ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were separated by SDS-PAGE electrophoresis at 100 volts (V) for 1 h on 4–20% Criterion™ TGX Stain-Free™ Protein Gels (1656001, Bio-Rad) and 1x SDS-page running buffer (192 mM glycine ...
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bioRxiv - Plant Biology 2024Quote: ... Expression of Dicer-Like 2 (DCL2) (Glyma.09g025300) and Argonaute 1 (AGO1) (Glyma.09g167100) was assessed using iTaq Universal SYBR Green Supermix (BioRad, Hercules, CA, USA). To quantify F ...
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bioRxiv - Plant Biology 2024Quote: ... Diluted cDNA (1:50) was used as a template for quantitative PCRs using iTaq Universal SYBR Green Supermix (Bio-Rad, Hercules, CA, USA). Real-time detection of fluorescence emission was performed on a CFX384 Real-Time System (Bio-Rad ...
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bioRxiv - Developmental Biology 2024Quote: ... Goat anti-mouse antibody conjugated to horseradish peroxidase (HRP) was used as a secondary antibody (1:2000) for chemiluminescence detection (Clarity Western ECL Substrates, Bio-Rad, Hercules, CA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad, Hercules, CA, USA). After washing 3 column volumes with wash buffer (50 mM Tris ...
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bioRxiv - Evolutionary Biology 2020Quote: ... A temperature gradient of 25 to 70°C (heating rate 1°C per min) was applied using a MJ minicycler (Bio-Rad, Hercules, CA, USA). All experiments were performed triplicates.
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bioRxiv - Evolutionary Biology 2020Quote: ... touchdowns from 56 °C to 50°C for 1 min and 50 cycles with 95 °C for 30 s and 50 °C for 1 min in a CFX96 Touch Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Physiology 2019Quote: ... The remaining supernatant was diluted to 1.5 µg × µl−1^ total protein in lysis buffer and 4X Laemmli sample buffer (Bio-Rad Laboratories AB, Oslo Norway) containing 2-Mercaptoethanol ...
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bioRxiv - Plant Biology 2020Quote: Colonies from TAP agar plates were scraped and suspended in 600 μL liquid TAP media and adjusted to an optical density of 1 at 600 nm using a visible light spectrophotometer (SmartSpec 3000, Bio-Rad, Hercules, CA, USA). The Chlamydomonas suspension was used to inoculate TAP liquid media at a 1:250 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... The plate was sealed with a pierceable seal (4titude; Wotton, UK) for 3 s at 180°C by using a plate sealer (BioRad PX-1; CA, USA) and maintained at 4°C during the LC-MS measurement ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a cellulose acetate membrane with pores with a 0.2 μm diameter (Whatman ...
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bioRxiv - Microbiology 2020Quote: ... was performed to detect viral RNA using the CDC standard N2-based SARS-CoV-2 detection assay [35] that was validated for use with the qScript XLT 1-Step RT-qPCR ToughMix (Quantabio, Beverly, MA, USA) on a CFX96 real-time thermocycler (Bio-Rad, Hercules, CA, USA) using a 20 minute RT step and 45 cycle qPCR in a 20 μL reaction volume ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Biochemistry 2022Quote: ... the membrane visualization was facilitated by 1:1 chemiluminescence luminol enhancer solution and peroxide solution using the Bio-Rad imaging system (Molecular Imager® Chemidoc XRS and Bio-Rad imaging system).
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bioRxiv - Immunology 2022Quote: ... Amplification reactions contained a target specific fraction of cDNA and 1 μM forward and reverse primers in iQ™ SYBR® Green Supermix (Bio-Rad, 1708882). Fluorescence was monitored and analyzed in a CFX connect real-time PCR system (Bio-Rad) ...
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bioRxiv - Cell Biology 2022Quote: ... separated by 8% SDS-PAGE gels at 100V for 1 hour and then transferred to a 0.45 μm pore size nitrocellulose membrane (Bio-Rad, catalog number: 162-0115) at 0.2 A for 1.5 hours in an ice bath ...
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bioRxiv - Neuroscience 2020Quote: ... protein extracts were diluted into a final concentration of SDS 1% and were subjected to vacuum filtration through a 96-well dot blot apparatus (Bio-Rad Laboratories, Hercules, USA) containing a 0.2 μM cellulose acetate membrane (Whatman ...
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bioRxiv - Plant Biology 2021Quote: ... was performed with gene-specific primers (Supplementary Table 1) using EF1α as internal reference using the CFX Connect Real-Time PCR Detection System (Bio-Rad, Hercules, CA, USA) as previously described in (Zandalinas and Mittler ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... After overnight incubation membranes were washed three times with 1x TBST for ten minutes per wash and then incubated with a secondary goat anti-rabbit antibody (BioRad Cat#1706515, 1:10000) for one hour with rocking at room temperature ...
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bioRxiv - Immunology 2022Quote: ... cell debris was removed by centrifugation and clear supernatants were diluted 10-fold and subjected to anion exchange chromatography on gravity fed columns using AG-1 9 8 resin (formate form, 100–200 mesh size, Bio-Rad Laboratories, Hercules, CA). Fractions containing inositol mono ...