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Citations for Bio-Rad :
9201 - 9250 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... 4 ul of diluted cDNA (1:40 dilution rate) were used for each reaction with 5 ul SsoAdvanced Universal SYBR® Green Supermix (BIO-RAD) and 1 ul of primer mix (5uM) ...
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bioRxiv - Neuroscience 2022Quote: ... 1:4 & 1:8) and loaded in triplicates onto a nitrocellulose membrane assembled in a 96-well dot-blot apparatus (Biorad, Cat#1703938). The membrane was then stained with Ponceau (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... TMEM87A in buffer 1 was exchanged into soybean l-α-phosphatidylcholine (Soy PC, MillaporeSigma) with the addition of Biobeads SM2 (Bio-Rad) and an hour incubation at a protein:lipid ratio of 1:10 (corresponding to 0.4 mg purified TMEM87A and 4 mg of Soy PC lipid or 1:50 in buffer (5 mM HEPES pH 7.2 ...
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bioRxiv - Biophysics 2022Quote: ... Electrophoresis was carried out for 1 hour at 90 V using an electrophoresis system (mini–DNA SUB CELL, Bio-Rad, Mississauga, Canada). The DNA fragments were visualized in a Gel Doc XR+ Gel Documentation System (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... 40 cycles of 15 s 95°C and 1 min 60°C) was carried out using a CFX 96 (BioRad, Hercules, USA) qPCR machine ...
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bioRxiv - Neuroscience 2022Quote: ... macrophages were plated in a 24-well plate containing cover slips and incubated with the Magic Red cathepsin B substrate (1/250) (Bio-Rad, ICT938) for 2h ...
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bioRxiv - Neuroscience 2022Quote: ... then placed in 4% normal goat serum in 0.01M PBS blocking solution for 60 minutes with agitation followed by incubation with rat anti-CD68 (1:500; BioRad, cat no. MCA1957GA) and rabbit anti-MBP (1:1000 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Expression levels of ICAM-1 and IL6 mRNAs were quantified using SYBR Green-based primers and the iTaq master mix (Bio-Rad Inc) in the Bio-Rad CFX Real-Time PCR detection system (Bio-Rad Inc) ...
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bioRxiv - Immunology 2022Quote: The collected fractions were then pooled by the expected oligomeric state for both human milk and serum and again IgAs were captured on beads with a 1% blocking buffer background (Bio-Rad, Netherlands) and addition of the 7D8-IgA1 and 5D5v2-IgA1 at 0.2 μg/mL ...
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bioRxiv - Synthetic Biology 2019Quote: ... 3-HB and n-butanol were separated with 5 mM sulfuric acid as the mobile phase and one of two column conditions: (1) an Aminex HPX-87H or Fast Acids anion exchange columns (Bio-Rad Laboratories) at 35 or 55 °C and a flow rate of 0.6 ml min-1 or (2 ...
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bioRxiv - Microbiology 2020Quote: ... containing 2 g l-1 glucose and 1.32 mM K2HPO4 by treating prepared media with a universal chelator (Chelex® 100 Chelating Resin, Bio-Rad 1421253), filtering sterilizing away the Chelex resin ...
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bioRxiv - Microbiology 2021Quote: ... the gel was stained with 1 mg/mL ethidium bromide and visualized with the ChemiDoc XRS system (Bio-Rad Laboratories, CA, USA). Genetic similarities were measured using the Dice coefficient and the clustering was carried out by the unweighted-pair group method with arithmetic averages (UPGMA ...
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bioRxiv - Developmental Biology 2021Quote: ... Following 4x 10 min washes with PBS-T the membrane was incubated for 1 min with Clarity Western ECL Substrate (170-5060, Bio-Rad Laboratories) and imaged with Amersham™ Imager 600 (GE ...
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bioRxiv - Microbiology 2019Quote: ... and visualized with rabbit-α-FLAG (1:3,000) primary and goat-α-rabbit-HRP conjugated secondary antibodies on the ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... The separated protein was transferred to a nitrocellulose membrane in Towbin buffer 1× using the Mini Trans-Blot system (Biorad, United States) at 120mA for 120 minutes in ice cold conditions ...
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bioRxiv - Physiology 2019Quote: ... 1 cOmplete™ protease inhibitor cocktail EDTA free tablet) and protein concentrations determined using the DC protein assay (Bio-Rad, Hercules, CA). Oxygen Consumption Rate (OCR ...
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bioRxiv - Cell Biology 2021Quote: ... 12.5 ng of cDNA of each sample were pre-amplified using 1 μL of all primers mix (500 nM) in a T100 Thermal cycler (BioRad, Hercules, CA), using the following program ...
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bioRxiv - Cell Biology 2020Quote: ... and analyzed for the expression of the exosome production-related genes listed in Appendix Table 1 by RT-PCR on a CFX Connect Real-Time System (Bio-Rad Laboratories).
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bioRxiv - Physiology 2021Quote: ... were used for real-time PCR with gene-specific primers (Supplemental Table 1) and Tbp as a house keeping gene on a CFX96™ Real-Time PCR Detection System (Bio-Rad).
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bioRxiv - Immunology 2021Quote: ... Livers were paraffin-embedded and immunohistochemistry was performed on 5-micrometer sections using F4/80 antibody clone Cl:A3-1 (BioRad Cat. No. MCA497) and the VECTASTAIN Elite ABS HRP kit (Vector Laboratories ...
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bioRxiv - Immunology 2021Quote: ... the enzyme reaction was stopped with 50 μL of 1 M sulfuric acid per well and the absorbance was measured in Bio-Rad Model 550 microplate reader (Bio-Rad Laboratories). Sera were assayed in duplicates and antibody titer represents the last reciprocal serum dilution above blank.
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bioRxiv - Immunology 2019Quote: ... Upfront tagmentation of ~35,000 nuclei was performed for 1 h at 37°C in a C1000 Touch thermal cycler with 96-Deep Well Reaction Module (Bio-Rad, 1851197). Tagmented nuclei were mixed the master mix and loaded onto a Chromium Chip with Gel beads and partitioning oil on different wells ...
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bioRxiv - Neuroscience 2021Quote: Autaptic murine striatal neurons (DIV 15-18) were incubated with 1 μg/ml human GABAAR mAb or control antibody (anti-CD52, Bio-Rad, #HCA175) at 37°C for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were visualized with goat anti-mouse or goat anti-rabbit IgG secondary antibodies (1:5000) diluted in 2% Omniblot milk (AmericanBio) in 1X TBST using a chemiluminescence detection system (BioRad ChemiDoc MP).
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bioRxiv - Bioengineering 2022Quote: ... the cells were blocked with Bovine Serum Albumin (BSA) in DPBS for 30 min and then incubated with anti-His6 antibody (1:100, Bio-Rad, MCA1396) for 2 h at 25°C ...
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bioRxiv - Cell Biology 2022Quote: ... for 20 minutes treated with PBS containing 1 % w/v BSA and 0.1 % v/v Triton X-100 (Bio-Rad, München, Germany) for 30 minutes ...
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bioRxiv - Molecular Biology 2022Quote: ... RNA was quantified by nanodrop and cDNA was produced from 1□μg of purified RNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories). qPCR reactions were performed using the SsoFast Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... PCR products were run on a 1% Agarose gel containing .08% ethidium bromide and visualized using the ChemiDoc imaging system (BioRad, Hercules, USA). PCR amplicons were sanger sequenced (Psomagen ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were blocked using 5% BSA in PBS and incubated overnight at 4 °C with 1 or an appropriate combination of up to 3 of the following antibodies: rat anti-CD8 (1:500, catalog no. MCA609G; Bio-Rad Laboratories), rat anti-CD4 (1:1,000 ...
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bioRxiv - Molecular Biology 2022Quote: ... cDNA was diluted to 1:200 (snRNA KD) or 1:25 (motor neurons and EXOSC10 KD) and qRT-PCR was performed with Ssofast EvaGreen (Bio-Rad, 1725201) with the following cycling conditions ...
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bioRxiv - Neuroscience 2024Quote: ... pH 8.0, 150 mM NaCl, 1% NP-40 [United States Biological, 19628], 0.5% deoxycholate [Sigma-Aldrich, D6750], 0.1% SDS [Bio-Rad, 1610302]) containing protease inhibitor cocktail and phosphatase inhibitors (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
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bioRxiv - Genetics 2024Quote: ... at 75 V for 15 min and 100 V for 1 hr 15 min using 1X Tris/Glycine/SDS running buffer (BioRad Cat. # 1610772). Following electrophoresis ...
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bioRxiv - Neuroscience 2024Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... applying a constant current of 300 mA at 4°C for 1 hour using a Mini Trans-Blot® Cell (Bio-Rad). For the immunodetection of specific protein targets ...
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bioRxiv - Developmental Biology 2023Quote: ... Transfer buffer was prepared as a 1X final solution by mixing 7:2:1 of ddH2O: methanol: 10X Tris/Glycine Transfer Buffer (Bio-Rad #1610734). Following transfer ...
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bioRxiv - Immunology 2024Quote: ... A 3.3 mm DBS punch from each sample was combined with 250 µl elution buffer (Tris-buffered saline, 1% casein blocker) (Bio-Rad Laboratories) in a 96 well plate ...
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bioRxiv - Biochemistry 2024Quote: ... were annealed at a ratio of 3:1 molar ratio Incubated at 93°C for 3 minutes and slowly cooling down in C1000 Touch™ Thermal Cycler (Bio-Rad). The pre-annealed RNA-TS (32 µM ...
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bioRxiv - Microbiology 2023Quote: ... Tissues were rinsed in DBPS and moved to semi-solid media (6:4 ratio of 1% agarose (BIO-RAD, Hercules, CA, USA) in DPBS:DMEM with 10% FBS) ...
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bioRxiv - Bioengineering 2023Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... and 95 % humidity to let them attach and recover to a concentration of 1×106 cells/m to 20 million cells counted using an automated Cell Counter System (Countess TC20, Biorad, Hercules, CA) which assessed cell viability via trypan blue exclusion ...
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bioRxiv - Physiology 2023Quote: ... The blots were washed four times with 0.1% tween TBS before being incubated for 1 h at room temperature with goat Anti-rabbit IgG HRP conjugated secondary antibody (Bio-Rad, USA) in 0.1% tween TBS buffer containing 3% BSA (Bovogen ...
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bioRxiv - Pathology 2023Quote: ... Heparin Sepharose 6 Fast Flow beads (1 ml) (Healthcare Life Sciences) were loaded into disposable Bio-Spin® chromatography columns (Bio-Rad) and packed with 2 ml of equilibration buffer (0.15 M NaCl in 25 mM HEPES ...
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bioRxiv - Neuroscience 2023Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS solution with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Biochemistry 2023Quote: ... Secondary antibody incubation was done at room temperature for 1h (HRP-conjugated goat anti-rabbit IgG, 1:2000 dilution, Bio-Rad, 403005). Membranes were washed with Tris-buffered saline containing 0.05% (v/v ...
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bioRxiv - Bioengineering 2023Quote: ... before injecting into 1 mL Hitrap Heparin HP column pre-equilibrated with Wash Buffer C operated in the BioLogic DuoFlow™ FPLC system (Bio-rad). The protein was eluted from the column by running a gradient flow rate that exchanges Wash Buffer C and Elution Buffer D (2000 mM NaCl ...
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bioRxiv - Bioengineering 2022Quote: ... Levels of inflammatory cytokines were quantified using Bio-Plex Pro Human Inflammation Panel 1 37-plex (#171AL001M) magnetic bead-based assay (Bio-Rad Laboratories) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... Gel demonstrating protein purity was generated using by loading 1 μg of each protein onto a Mini-PROTEAN TGX 4-20% gradient gel (BIO-RAD, 4561096) and then staining with Coomassie blue.
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bioRxiv - Biophysics 2023Quote: ... The mixture was incubated for 1-2 h at 4 °C and incubated overnight with pre-washed bio-beads (Bio-Rad Laboratories) at 4 °C ...