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Citations for Bio-Rad :
9151 - 9200 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... 20 minutes) and the clear lysate was purified on HisTrap HP 1 ml affinity column (Cytiva) by FPLC system (NGC™, BioRad). The protein was eluted from the column using a linear gradient (Elution buffer ...
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bioRxiv - Biochemistry 2022Quote: An equivalent volume of ∼150 µg FBS was first diluted 1:2 with loading buffer (consisting of 2X Laemmli Sample Buffer (#1610737, Bio-Rad) plus a 5% (v/v ...
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bioRxiv - Microbiology 2023Quote: ... flash frozen in liquid nitrogen and treated with a lysis buffer (150mM NaCl, 50mM Tris pH7.5, 1% Triton X-100 (Bio-Rad #1610407), 1% SDS ...
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bioRxiv - Genetics 2023Quote: ... China) using TBE (65 mM Tris-HCl, 27 mM boric acid, 1 mM EDTA, pH 9; Bio-Rad, Hercules, CA, USA) as the buffer ...
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bioRxiv - Biochemistry 2024Quote: ... Gels were then stained with Coomassie Brilliant Blue G (80 mg dissolved in 1 L water with 30 mM HCl) and destained with water before visualising using a ChemiDoc MP Imaging System (Bio-Rad). For mNeonTP-loaded encapsulins ...
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bioRxiv - Synthetic Biology 2024Quote: The samples (1 µL) were subjected to SDS-PAGE and transferred to a nitro cellulose membrane (Bio-Rad Laboratories, CA, USA). The membrane was blocked using 3% skim milk in PBST (137 mM NaCl ...
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bioRxiv - Plant Biology 2024Quote: pTRV1 and pTRV2-Del/Ros-1 harboring the SlATG7- or SlATG2-derived fragments were introduced into Agrobacterium strain GV3101 using electroporation (BIO-RAD). A 5 mL culture was grown overnight at 28°C in a medium containing kanamycin ...
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bioRxiv - Biochemistry 2024Quote: ... Nanodisc formation was initiated by adding washed Bio-Beads (200 mg dry weight per 1 mL of the protein mixture; Bio-Rad), and the mixture was rotated for two hours at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: Proteins from SDS-PAGE gels were transferred onto a PVDF (polyvinylidene difluoride) membrane (pre-activated in methanol for 1 min) using Trans-Blot Turbo transfer system (Bio-Rad) according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2023Quote: ... 20 μL of PCR product was loaded and run on a 1% (weight/volume) agarose gel and imaged with the ChemiDoc Touch Imaging System (Bio-Rad).
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bioRxiv - Microbiology 2023Quote: ... brucei PFs were prepared in 1% Triton X-100 in PBS with 1X protease inhibitor cocktail (Bio-Vision) and mixed in 4X Laemmli buffer (Bio-Rad) with 710 mM β-mercaptoethanol and heated for 5 min at 95°C ...
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bioRxiv - Bioengineering 2024Quote: ... PCR products were mixed 1:6 with a loading dye and run on an agarose gel (1%) in Tris-acetate-EDTA buffer supplemented with 0.2 µG/mL of Ethidium Bromide (Biorad, Hercules, California). DNA was visualized under a short-wavelength UV light to confirm the lack of amplification for Heat-Killed Microbiome PMA-treated in comparison to the Heat-Killed Microbiome control (Supplementary Figure 2).
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bioRxiv - Physiology 2024Quote: ... was used to wash the membranes before incubating with goat anti-rabbit horseradish peroxidase conjugated secondary antibody at 1:5000 dilution (1706515, Bio-Rad) for 90 min at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... 1 µg of total RNA from each sample was converted to cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708841). Then qPCR reactions were set up using TaqMan® Fast Advanced Master Mix (ThermoFisher Scientific ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times with and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... After gel polymerization was performed pre-electrophoresis of empty gel in 5% acetic acid (running buffer) for 1-1.5h/150 V in reverse polarity of electrophoretic BioRad system (Bio-rad, USA). The running buffer was discarded ...
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bioRxiv - Developmental Biology 2024Quote: ... Proteins were separated on 10 % polyacrylamide gels and blotted onto a nitrocellulose membrane at 100 V for 1 h using the Mini Trans-Blot system (Bio-Rad). Membranes were blocked with 5 % milk powder in PTw (1x PBS ...
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bioRxiv - Microbiology 2024Quote: ... Each well was then blocked with 150 µL of blocking buffer consisting of 1×PBS + 4% (w/v) nonfat milk (Bio-Rad). The plates were blocked for 1 h at room temperature and then washed five times with PBST ...
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bioRxiv - Cancer Biology 2024Quote: ... Secondary antibodies were applied for 1 hour at room temperature (1:5,000; CST, #7074) before detecting protein bands at the BioRad ChemiDoc MP Imaging System using chemiluminescence (BioRad, 170-5061).
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bioRxiv - Genetics 2024Quote: ... cDNA was diluted 1:4 with water and 2ul used as template for qPCR using 250nM primers using the SsoFast EvaGreen Supermix (BioRad 1725200) on an Applied Biosystems ViiA 7 Real Time PCR System ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples from the co-IP and were separated on 8% SDS-PAGE gels for 1 h at 120 V and transferred overnight at 20 V on to PVDF membranes (Bio-Rad). Blots were probed with rabbit anti-NMIIB/Myh10 (1:2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... The HRP-linked secondary antibodies (CST: 7076S) were used by 1:4000 dilution and the blots were visualized with the ECL reagents (Bio-Rad).
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bioRxiv - Physiology 2024Quote: ... Blotted bands were detected using HRP conjugated secondary antibodies goat anti-rabbit conjugated with HRP (#1706515, Bio-Rad, 1:15, 000). ImageJ was used to calculate the fluorescence density of each band ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 ng template library DNA[21] in a total reaction volume of 20 µL using T100 Thermal Cycler (BIO-RAD). The PCR amplification cycle was as follows ...
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bioRxiv - Cancer Biology 2024Quote: ... was added to each sample and heated at 95 °C for 1 hour in a thermal cycler (S1000 with 384-well reaction module, Bio-Rad) set to a constant lid temperature of 110 °C ...
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bioRxiv - Biophysics 2024Quote: ... the GRB2 protein was eluted by linearly increasing the concentration of Imidazole from 20 to 500 mM with a flow rate of 1 ml/min in buffer A using Econo Gradient Pump (BIO-RAD). The fractions containing the target protein were concentrated to 5 ml with Amicon Ultra-15 10 kDa (Merck) ...
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bioRxiv - Neuroscience 2024Quote: ... Viability of magnetically-captured cells (eluted into a new tube away from the magnet) was assessed by trypan blue staining (1:2 dilution sample in trypan blue; BioRad 1450013). Cell viability was quantified as the proportion of cells that did not take up the dye ...
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bioRxiv - Biochemistry 2024Quote: ... RNA concentration was measured and 1 μg was used to synthesize cDNA using iScript™ Reverse Transcription Supermix (Bio-Rad, 1708891) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... After final wash and supernatant removal, 1x Loading Buffer (40 μL, 3:1 Lysis Buffer:4x Laemmli Loading Buffer (Bio-Rad)) was added to the sample and the sample boiled (10 min ...
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bioRxiv - Microbiology 2024Quote: ... The cell mixture was transferred to a 1 mm electroporation cuvette and pulsed using a Gene Pulser Xcell Electroporation System (Bio-Rad) using 2.1 kV/cm ...
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bioRxiv - Molecular Biology 2024Quote: ... was added to all samples and the products were separated on 15% polyacrylamide denaturing urea gels (19:1 acrylamide-bisacrylamide, Bio-Rad). The gels were fixed in fixing solution (40% methanol ...
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bioRxiv - Plant Biology 2020Quote: ... The cDNA was used as template for real-time PCR (primers in Supplemental table 1) using CFV384 Touch™ Real-Time PCR Detection System (Bio-Rad). Housekeeping genes (AT4G34270 ...
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bioRxiv - Biophysics 2021Quote: The removal of DDM in the protein solutions was done by fixing the protein concentration at 1 mg/ml and using 30 mg of BioBeads SM2 (Bio-Rad, CA) per mg of protein ...
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bioRxiv - Neuroscience 2021Quote: ... slices were bombarded with biolistic particles created by coating 1.6 μm gold particles (Bio-Rad, #165-2262; ∼1 μg DNA/mg gold) with constructs in mammalian expression plasmids ...
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bioRxiv - Developmental Biology 2020Quote: ... 1-20μg of tissue protein to be analyzed were combined with equal parts 2X Laemmli sample buffer (Bio-Rad, catalog 161-0737) containing 10% β-mercaptoethanol ...
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bioRxiv - Physiology 2020Quote: ... Gene expression was assessed using commercially available Taqman primer/probe sets (Thermo Fischer) or specific oligonucleotide pairs (Table 1) and SYBR® Green supermix (Bio-Rad). Relative expression of the target genes was calculated with the comparative Ct method [43] using glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Immunology 2021Quote: ... in 0.1% BSA in 0.1% TBST and subsequently imaged using HRP-conjugated anti-rabbit antibodies (Bio-Rad Cat#172-1019, 1:10000) and Western Lightning Plus-ECL (PerkinElmer Cat#El103001EA ...
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bioRxiv - Microbiology 2021Quote: ... Gels were stained with ethidium bromide (0.5 mg mL-1) and photographed using the Quantity One electrophoresis analysis system (Bio-Rad, CA, USA). Gel images were digitally normalized to a single DNA marker to reduce gel-to-gel banding pattern variability ...
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bioRxiv - Biochemistry 2021Quote: ... Gel electrophoresis was performed on a 1% agarose gel and imaged using Molecular Imager® Gel Doc™ XR (Bio-Rad, USA).
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bioRxiv - Systems Biology 2021Quote: Samples were normalized to 1 mg/mL protein in 0.1% Azo by the Bradford assay (Bio-Rad, Hercules, CA, USA, Cat# 5000006) using bovine serum albumin to generate a standard curve ...
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bioRxiv - Plant Biology 2020Quote: ... or 1 μl (for the rest) of a 1/2 dilution of the cDNA was added to a reaction containing 1X of SsoFast EvaGreen (Bio-Rad, USA), 0.5 μM Forward and Reverse primers (Table 2) ...
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bioRxiv - Plant Biology 2020Quote: ... we used 2 µl of a 1/5 dilution of the cDNA obtained as above in a reaction containing 5 µl of SsoFast EvaGreen (Bio-Rad, USA), 0.5 µl of Forward primer (10 µM ...
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were washed three times in TBST and incubated with horseradish peroxidase (HRP)-conjugated goat anti-rabbit (Bio-Rad 1,706,515, 1:10,000) secondary antibodies for 2 h at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Ribonucleoprotein (RNP) complexes were made at a 9:1 ratio of sgRNA:Cas9 (90pmol:10pmol) in Gene Pulser® Electroporation Buffer (Bio-Rad, 1652677) and incubated for 10 min at RT ...
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bioRxiv - Cell Biology 2021Quote: ... First-strand cDNA was synthesized from 1 μg of total RNA using iScript Reverse Transcription Supermix for RT-PCR (Bio-Rad Laboratories) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... All qPCR amplifications were performed in duplicate and each contained 15 µL total volume of 1 × SsoAdvanced Universal SYBR Green Supermix (Bio-Rad, USA), 0.3 µM of each primer ...
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bioRxiv - Cancer Biology 2021Quote: ... 60 of High Capacity NeutrAvidin slurry (Thermo PI29204) were washed three times with 1 mL 2X RIPA/1mM EDTA buffer in 2-mL chromatography columns (Bio-Rad 7326008) attached to a vacuum manifold (Promega A7231) ...
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bioRxiv - Microbiology 2022Quote: ... The melt curve analysis was performed at 65°C for 1 minute in CFX connects TM Real-time PCR detection system (Bio-Rad, USA). The primers used are listed inTable 1 ...
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bioRxiv - Microbiology 2022Quote: ... Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was detected by using a 1:1,000 anti-GAPDH hFAB™ Rhodamine antibody (Bio-Rad, Hercules, CA) in 5% milk TBST (Tris buffer saline plus Tween-20) ...
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bioRxiv - Microbiology 2022Quote: ... 300 nM concentration of each primer targeting the viral DNA substrate (5’- AGCGTGGGCGGGAAAATCTC-3’) and the indicated target DNA (table 1) and 1X iTaq Universal SYBR Green Supermix (Bio-Rad Laboratories). The qPCR cycling conditions for quantifying INS activity included an initial incubation at 95°C for 3 minutes ...