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Citations for Bio-Rad :
9051 - 9100 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... Products were separated by electrophoresis on a 0.7% agarose gel run in 1× TAE buffer at 55 V in the cold room and imaged on a ChemiDoc Touch instrument (Bio-Rad).
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bioRxiv - Biophysics 2020Quote: ... supplemented with DTT (0.1 M endconcentration) and then subjected to SDS-PAGE on 12% or 4–20% gradient gels (mini-PROTEAN TGX; Bio-Rad). Proteins were transferred to PVDF membranes (TransBlot® TurboTM LF PVDF ...
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bioRxiv - Developmental Biology 2020Quote: ... USA) and 1 μg of RNA was reverse transcribed by using 5X iScript Reverse Transcription Supermix (Bio-Rad, Mississauga, ON, Canada) according to the manufacturer’s instructions.
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bioRxiv - Molecular Biology 2021Quote: ... The formaldehyde crosslinks were reversed by heating the samples for 1 h at 70 °C and RNA was extracted using PureZOL™ (BioRAD) as described in the RNA extraction section ...
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bioRxiv - Microbiology 2019Quote: ... Expression of AcrIIA1 mutants was confirmed by harvesting 1 OD600 unit of cells and resuspending in 200 µl 1X Laemmli Sample Buffer (Bio-Rad) followed by SDS-PAGE and immunoblotting as described above ...
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bioRxiv - Microbiology 2020Quote: The quality of genomic DNA and the PCR products were checked on 1% (w/v) agarose gel made by dissolving 1.5 g of agarose (Bio-Rad, SA) in 150 ml of 1X Tris-acetate-ethylenediaminetetraacetate (TAE ...
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bioRxiv - Microbiology 2020Quote: ... The PCR products were identified via 1% agarose gel electrophoresis and visualized in a Gel Doc system (BioRad, Hercules, CA, USA).
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bioRxiv - Microbiology 2020Quote: ... A total of 100μL competent cells were incubated in a 1mm cuvette with 1–8μg DNA for 20 min on ice prior to pulsing in a GenePulser Xcell™ electroporator (Bio-Rad) with time constant and voltage settings at 5 ms and 1200V ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were incubated for one hour either with a 1:5 dilution of Rat anti Human CD3:Alexa Fluor®647 (0.05 mg/mL) (clone CD3-12; Bio-Rad) or with undiluted CD8 (AM22 ...
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bioRxiv - Molecular Biology 2020Quote: ... cDNA was generated using 1 µg total RNA and random hexamers with SuperScriptTM III Reverse Transcriptase and qPCR performed with iQTM Supermix (Bio-Rad) using a CFX96 Real-Time Thermocycler (Bio-Rad ...
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bioRxiv - Genomics 2021Quote: ... The indexed amplicons were mixed based in relative fluorescence units and run on a 1% agarose gel with Sybr Safe and gel extracted using a freeze and squeeze column (Bio-Rad). The product was quantified using KAPA Library Quant kit (KAPA Biosystems).
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bioRxiv - Genetics 2020Quote: ... After washing and incubation with a secondary antibody and 1 × TBST washes the membrane was coated with chemiluminescent substrate (Clarity Max ECL substrate, Bio-Rad) and imaged on Chemidoc MP (Bio-Rad).
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bioRxiv - Genetics 2020Quote: ... 1 μL diluted cDNA (5 ng) in a total volume of 5 μL using a CFX384 Real-Time System (Bio-Rad). For each experimental sample (four source colonies ...
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bioRxiv - Physiology 2021Quote: ... cDNA was diluted 1:5 and then 2μl was added to 5ul SYBR green PCR mixture (BioRad, 1725271, Hercules, CA, USA), 2.4ul water and 1.25 pmol primer mix ...
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bioRxiv - Biochemistry 2021Quote: ... both used at a concentration of 1:200 were followed by a one-hour incubation with secondary Goat anti-(Rabbit IgG-HRP conjugate) (#1706515, Bio-Rad) at a concentration of 1:8000 at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated for 1 h at 4°C with 0.5 mL Profinity IMAC resin (Bio-Rad Laboratories, Hercules, CA) in a buffer containing 20 mM MOPS-Na (pH 7.4) ...
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bioRxiv - Biochemistry 2021Quote: ... 12 μL of precleared samples were added to 12 μL of beads in PBS-casein (PBS containing 1% casein, Bio-rad Laboratories ...
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bioRxiv - Biochemistry 2021Quote: ... and increasing concentrations of NONO(53-312) were prepared and incubated at 4°C for 1 hour before loaded onto a Dot Blot apparatus (Bio-rad) containing a nitrocellulose membrane (top ...
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bioRxiv - Cell Biology 2022Quote: ... The cDNA was diluted 1:20 prior to detection of GADD34 pre-mRNA transcripts using iTaq Universal SYBR Green (Bio-Rad). In short ...
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bioRxiv - Bioengineering 2021Quote: ... Acrylamide/bis solution (30%) 29:1 and natural polypeptide sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) standard were purchased from Bio-Rad. Agarose was purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... the integrity of bacmid DNA was analyzed by digestion with restriction enzyme XhoI and separation in 1% PFGE agarose (Bio-Rad) gels and 0.5×TBE buffer by pulsed-field gel electrophoresis at 6 V/cm ...
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bioRxiv - Cancer Biology 2021Quote: ... VNR was detected on cells fixed in cold methanol by CD51/61 immunocytochemistry (clone 23C6, 1:400; Bio-Rad, Oxford, UK). Resorption tracks on dentine discs were visualised by staining with 0.5% toluidine blue under reflected light ...
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bioRxiv - Cell Biology 2021Quote: ... but we have observed better suppression of autofluorescence at 1%.) Cells were further quenched and washed with 50 mM glycine (Bio-Rad) in DPBS (DPBS-G ...
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bioRxiv - Microbiology 2021Quote: ... incubated with specific primary antibodies for 1 h and detected with horseradish peroxidase (HRP)-conjugated secondary antibodies by using ClarityTM Western ECL Blotting Substrate (Bio-Rad). For pulldown assays between cell extract and purified Nyx and MAG mutants ...
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bioRxiv - Microbiology 2020Quote: The supernatants from the different lysis buffer extractions described above were diluted 1:4 in 4x Laemmli sample buffer (Bio-Rad) supplemented with beta-mercaptoethanol following manufacture’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... 10.5 μl template DNA and 1 μl respective primer pairs (200 nM final concentration, supplementary table 1) were mixed and distributed into a 96-well qPCR plate (Bio-Rad). The plate was incubated 10 min at room temperature to digest the target DNA before droplets were generated in an Automated Droplet Generator (Bio-Rad) ...
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bioRxiv - Microbiology 2020Quote: ... Gel was stained with SYBR Gold (diluted 1:10000 in TBE buffer) for ∼30min in the dark and imaged on a ChemiDoc MP Imaging System (Bio-Rad).
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bioRxiv - Biochemistry 2020Quote: ... in TBST/5% wt/vol non-fat milk (1 h, RT), washed (5x, TBST) followed by addition of ECL substrate (Bio-Rad) and imaged (ChemiDoc ...
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bioRxiv - Biochemistry 2020Quote: ... the anti-rabbit secondary antibody was incubated for 1 h and chemiluminescence was measured using a ChemiDoc XRS+ imaging system (Bio-Rad) after development in chemiluminescence reagent (Westar Nova 2011 ...
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bioRxiv - Molecular Biology 2021Quote: ... Digitalized images of the destained SDS-PAGEs were analyzed by the Quantity One 1-D Analysis Software (Bio-Rad, Berkeley, US) to measure the band densities at each lane of all biological replicates ...
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bioRxiv - Immunology 2020Quote: ... at 1:20,000 dilution in PBS with 1% (w/v) skimmed milk and 0.1% (v/v) Tween-20 or goat anti-porcine IgA HRP (Bio-Rad Antibodies) at 1:20,000 dilution in the same diluent.
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bioRxiv - Immunology 2021Quote: Protein analysis was performed using Bioplex assays (27-Plex, CXCL-1-, CXCL-2-, CXCL-5-, CCL22-single plex) (Bio-Rad Laboratories) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2021Quote: ... The primary antibody was removed the next day and the membrane was incubated with secondary antibody at room temperature for 1 h. Enhanced chemiluminescence substrate (Clarity Western ECL Substrate, Catalog no. 1705060) from Bio-Rad Laboratories (Mississauga ...
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bioRxiv - Genetics 2020Quote: ... Collected protein samples were boiled with 1× Laemmli buffer before SDS-PAGE procedure with Mini-PROTEAN TGX Precast Gel (Bio-Rad) in Tris/Glycine Buffer (Bio-Rad) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were added to the control wells without cells (Lanes 1 and 12) ranging from 0.125 – 2 mg/ml (BioRad 500-0202) and treated with formalin and NaOH at the same concentrations as the cells ...
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bioRxiv - Microbiology 2021Quote: ... for 1 h at room temperature and subsequently probed with appropriate secondary antibodies coupled to Horseradish peroxidase for 1 h at room temperature followed by chemiluminescent detection with ECL reagent (Bio-Rad).
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bioRxiv - Microbiology 2021Quote: ... Each well was then coated with 150 µl of a blocking buffer consisting of PBS and 40 mg ml-1 blotting-grade blocker (Bio-Rad). The plates were incubated with the blocking buffer for 1 hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... This solution was injected into a microfluidic drop maker (device 1) along with Biorad droplet generation oil for Evagreen (Biorad #1864005) with 0.5% v/v TEMED (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... Fluorescent secondary antibodies (1:15,000) were incubated for 1 hour at room temperature prior to fluorescence detection using a ChemiDoc™ Imaging System (Bio-Rad). Quantification of band densitometry was performed using the FIJI ImageJ software (51).
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bioRxiv - Biophysics 2022Quote: ... Resulting membranes were resuspended in 100 μL solubilization buffer (1xPBS, 1% DDM, EDTA-free protease inhibitors) and total protein concentration was quantified using DC protein assay (Bio-Rad). For each condition ...
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bioRxiv - Bioengineering 2022Quote: ... ethidium bromide at a final concentration of 0.46 μg mL−1 was used and the DNA was visualized under UV light using a GelDoc XR+ system (Bio-Rad).
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bioRxiv - Cancer Biology 2022Quote: ... 0.1% SDS) and transferred onto nitrocellulose membranes (Protran) with a Trans-Blot SD semi-dry or wet transfer cell (Bio-Rad). Proteins of interest were probed using the appropriate primary and Horse Radish Peroxidase (HRP)-conjugated secondary antibodies (Jackson Immunoresearch) ...
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bioRxiv - Genetics 2022Quote: ... Membranes were washed three times with PBS-T for 5 min followed by 1 minute incubation with ECL detection reagent (Cytiva, RPN2106) and imaged with ChemiDoc MP Imaging System (Bio-Rad). Membranes were washed twice in TBS and stripped before reprobing according to stripping reagent manufacturer’s protocol (Thermo Scientific ...
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bioRxiv - Genomics 2022Quote: ... and incubated at 65°C for 3 mins before running 1% agarose-Etbr gel electrophoresis to detect the cleavage efficiency by ChemiDoc system (Bio-Rad).
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bioRxiv - Immunology 2022Quote: ... then blocked for 1 to 3 hr at RT with 50 μL/well of blocking buffer (HN: 5% Blotting Grade Blocker, Bio-Rad cat ...
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bioRxiv - Immunology 2022Quote: ... and primers to the editing locus producing amplicons of <500bp (1× assay, 900-nM primers and 250-nM probe) using ddPCR supermix for probes (no deoxyuridine triphosphate [dUTP]) (Bio-Rad). Reference reactions were simultaneously performed using a 5’ HEX labeled probe/ primer combination targeting the houskeeping gene B2M ...
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bioRxiv - Plant Biology 2022Quote: ... SybrGreen real-time quantitative PCR experiments were performed with a 1:20 dilution of cDNA using a CFC384 Real-Time System (Bio-Rad). Data were analyzed with the comparative 2ΔΔCt method using the geometric mean of Ef1α (5’-CTG GAG GTT TTG AGG CTG GTA T-3’ and 5’-CCA AGG GTG AAA GCA AGA AGA-3’ ...
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bioRxiv - Microbiology 2022Quote: ... Plates were then washed six times with PBS-T and incubated with horseradish peroxidase-conjugated goat anti-rabbit IgG (H + L) (1:2000) (Bio-Rad) diluted in 2% horse serum/PBS for 1 hr at room temperature with agitation ...
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bioRxiv - Microbiology 2022Quote: ... and the RNA profile was measured by absorbance (254 nm) across the gradient in real time using an EM-1 Econo UV Monitor (Bio-Rad).
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bioRxiv - Microbiology 2022Quote: ... and probed in triplicate by qPCR for the presence of the 1 kb capsule fragment using iTaq DNA polymerase (BIO-RAD) and primer pair DDL34-DDL35 ...