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Citations for Bio-Rad :
8951 - 9000 of 9407 citations for Mouse Interleukin 1 Family Member 10 IL1F10 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... Quantitative RT-PCR was carried out using the PerfeCTa SYBR Green Fastmix (Quantabio, 95071) and gene-specific primers (Table 1) on CFX96 system (Biorad). Relative gene expression was determined using the ΔΔCt method.
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bioRxiv - Genetics 2024Quote: ... followed by purification and size selection on a 1% agarose gel with 0.6 µg/mL ethidium bromide (BioRad Cat#1610433), selecting for the 7,961 bp band ...
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bioRxiv - Cancer Biology 2024Quote: ... The HRP-coupled secondary antibodies were used at a 1:10,000 dilution followed by development using ECL substrate from Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2024Quote: ... Membranes were stained with Ponceau S to visualize protein loading and VAChT was revealed with rabbit anti-VAChT (1:1000, SYSY, 139103) by ECL procedure (Clarity Western ECL, Biorad) on a chemiluminescence imager (ChemiDoc ...
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bioRxiv - Bioengineering 2024Quote: ... and polystyrene disposable cuvettes (Semi-Micro, 1 cm path, 1.5 mL, 12.5 mm square x 45 mm high; Bio-Rad, 2239955). The alginate and CaSO4 were mixed ...
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bioRxiv - Biochemistry 2024Quote: ... 50 µL of the cell-oligonucleotide mixture was transferred to a pre-chilled 1 mm electroporation cuvette (BioRad catalog #1652083) and transformed with 1.8 kV ...
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bioRxiv - Molecular Biology 2024Quote: ... 60 mM EDTA pH 7.0 containing 5% β-mercaptoethanol and 1 mg/ml zymolyase 100T) at 40 °C and aliquoted to plug molds (BioRad) to prepare 20 agarose plugs ...
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bioRxiv - Neuroscience 2024Quote: ... Proteins were electrotransferred onto nitrocellulose membranes using the trans-blot turbo transfer system (constant voltage: 25V, maximum amperage: 1 A; 30 min, Biorad) and membranes were blocked with Tris-buffered saline containing 0.1% Tween 20 (TBST) ...
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bioRxiv - Cell Biology 2024Quote: ... HRP-conjugated secondary antibodies were used at 1:5000 dilutions and chemiluminescence signal was produced using the Clarity western ECL substrate (BioRad) and detected using a ChemiDoc imaging system (BioRad) ...
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bioRxiv - Molecular Biology 2024Quote: ... The reaction was resolved by electrophoresis on a 6% non-denaturing polyacrylamide gel in TBE (100 mM Tris, 90 mM boric acid, 1 mM EDTA) buffer and visualised by ChemiDoc (BioRad) using SYBR Gold stain or imaging in the 6FAM ...
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bioRxiv - Physiology 2024Quote: ... Membranes were then washed three times and incubated with StarBright Blue 700 goat anti-rabbit IgG (1:3500; Bio-Rad) for 1 h ...
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bioRxiv - Cell Biology 2024Quote: ... blots were incubated in 1-shot ECL solution (Kindle Biosciences LLC) and imaged with a ChemiDoc MP Imaging System (BioRad).
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bioRxiv - Biophysics 2021Quote: To extract lipids, purified SaPgsA (5.82 mg ml-1, 200 μl) was mixed with 40 mg SM2 Biobeads (Bio-rad, USA) and gently shaking overnight at 4 °C to remove detergent ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.2–2.2 Mb, S. cerevisiae Ladder: Bio-Rad, 1703605; CHEF DNA Size Marker, 1–3.1 Mb, H. wingei Ladder: Bio-Rad, 1703667) and PFGE was performed using the CHEF Mapper XA System (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: ... For immunohistochemistry (IHC) experiments following primary antibodies were used: IgG anti-human CD3 antibody (1:200, clone CD3-12, Bio-Rad) and anti-PCNA antibody (1:1000 ...
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bioRxiv - Developmental Biology 2020Quote: ... Stain-Free gels were activated with UV light for 1 minute and imaged for total protein on gel using a ChemiDoc MP imager (Bio-Rad). Proteins were transferred to PVDF blotting membranes (Bio-Rad ...
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bioRxiv - Cell Biology 2019Quote: ... we used 1/20th volume of a cDNA reaction to program a PCR reaction using iQ SYBR Green Supermix (Bio-Rad). PCRs were amplified on a CFX96 Real-Time System (Bio-Rad ...
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bioRxiv - Cell Biology 2020Quote: ... The samples were then subjected to SDS-PAGE at a constant voltage of 100 V for 1 hour at room temperature and transferred to nitrocellulose membrane (BIO-RAD) at a constant voltage of 30 V at 4°C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... The product yield for each condition was estimated by resolving 10% of the reaction on a 1% EtBr-agarose gel and quantifying the band intensity of the resulting amplicon with ImageLab software (Bio-Rad). The 1.6-kb amplicon was gel-extracted using the QiaQuick Gel Extraction Kit (Qiagen ...
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bioRxiv - Cell Biology 2020Quote: ... Signals were developed by incubating with 1/5000 fold dilution of anti-rabbit or anti-mouse secondary antibodies for 1 h at room temperature and quantified using the Image Lab Software on a ChemiDoc XRS system (Bio-Rad).
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bioRxiv - Cell Biology 2020Quote: ... Reverse transcription was performed with 1 μg total RNA using iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad 170-8841). cDNA equivalent to 20 ng of total RNA was then amplified in duplicate using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 172-5274 ...
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bioRxiv - Cell Biology 2020Quote: ... They were immediately transformed by bombardment with plasmid-coated 1-μm gold particles with a helium-driven particle accelerator (PDS-1000/He; Bio-Rad) using 1350 psi rupture disks and a vacuum of 28 inches of mercury ...
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bioRxiv - Developmental Biology 2021Quote: ... and OPN) and control (HPRT) transcripts were amplified (Supplemental Table 1) using SsoAdvanced Universal SYBR Green Supermix (Biorad, Hercules, CA, USA). Established TaqMan assays were used to determine the expression of Lgr6 and TBP intra-well controls ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated by electrophoresis at 70V for 15 minutes followed by 100V for approximately 1 hour using 10x Tris/Glycine/SDS running buffer (BioRad, 1610772).
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bioRxiv - Microbiology 2019Quote: ... The copy number of a host gene RPP30 was determined using 1 μL of HEX-labeled PrimePCR RPP30 (Bio-Rad, 10031243) in the reaction to calibrate the amount of DNA input ...
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bioRxiv - Molecular Biology 2021Quote: ... and transferred to nitrocellulose membrane (Whatman Protran BA85, cat. No. 10401196) (25 V, 1 A, 3 0min using Trans Blot Turbo transfer system (Bio-Rad). Membrane was blocked with 5% milk and probed with anti-mAID (1:5,000 ...
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bioRxiv - Molecular Biology 2020Quote: ... The deamination reaction was then carried out by incubating in a thermal cycler for four cycles of 5 minutes at 70°C followed by 1 hour at 60°C and then desalted with Micro Bio-spin 6 chromatography columns (Bio-Rad). RNA was desulphonated by adding an equal volume of 1 M Tris (pH 9.0 ...
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bioRxiv - Molecular Biology 2020Quote: Following electrophoretic separation, oligonucleotide-protein complexes were transferred by electro-blotting (330 mA, 1 h) onto specific positively charged nylon membranes (Bio-Rad) and detected using an anti-digoxigenin antibody conjugated with alkaline phosphatase.
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bioRxiv - Genomics 2020Quote: ... The indexed amplicons were mixed based in relative fluorescence units and run on a 1% agarose gel with Sybr Safe and gel extracted using a freeze and squeeze column (Bio-Rad). The product was quantified using Kapa Illumina Quant kit.
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Histone deacetylase inhibitors butyrate and bufexamac inhibit de novo HIV-1 infection in CD4 T-cellsbioRxiv - Microbiology 2020Quote: ... supplemented with 1 mM DTT for reduction of disulfide bonds and protein concentration was determined by Quick-start Bradford assay (Bio-Rad). Proteins were alkylated with 5.5 mM chloracetamide (45 min ...
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bioRxiv - Cancer Biology 2019Quote: ... cDNA was produced from 1 μg of purified RNA using the iScript Reverse Transcription Supermix for RT-qPCR (Bio-Rad Laboratories). qPCR reactions were performed with the SsoAdvanced Universal SYBR Green Supermix (Bio-Rad Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: ... secondary antibodies were then incubated for 1 hour at room temperature followed by detection of bands by chemiluminescence in ChemiDoc XRS+ (Bio-Rad).
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bioRxiv - Neuroscience 2019Quote: ... 0.02% NaN3 and 1 mM DTT at pH 7.4 at 37°C in a thermal cycler (BioRad DNA Engine PTC-200). In some cases ...
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bioRxiv - Microbiology 2019Quote: ... Alternatively broken cyst walls either before or after digestion with trypsin were reconstituted in 1× reducing SDS/PAGE loading buffer and run on a 4–20% precast polyacrylamide TGX gel (Bio-Rad). Bands stained by colloidal Coomassie blue were excised and washed with 50 mM NH4HCO3/acetonitrile (ACN) ...
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bioRxiv - Genomics 2019Quote: Differentiating and IFC-305 treated HepaRG cells cultured for 1 week were trypsinized and counted using TC20 automated cell counter (Bio-Rad) with dual chamber slides and trypan blue (Bio-Rad 1450003) ...
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bioRxiv - Microbiology 2019Quote: ... The libraries were quantified and checked for amplifiable adapters using the Library Quantification DNA standards 1-6 (Kappa Biosystems, Wilmington, USA) with the SsoFast EvaGreen qPCR supermix (Bio-Rad) using 10 μL EvaGreen master mix ...
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bioRxiv - Neuroscience 2019Quote: ... incubated overnight with primary antibody in 5% milk in TBST at 4°C and subsequently washed in TBST and incubated for 1 hr at room temperature with HRP-linked secondary antibody (Bio-Rad) in 5% milk in TBST ...
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bioRxiv - Microbiology 2019Quote: ... 250 µl packed cells were combined with 250 µl of 1 × Cytomix in a 2 mm transfection cuvette (Bio-Rad Laboratories). The precipitated and resuspended DNA was added to the cell suspension in the cuvette ...
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bioRxiv - Zoology 2019Quote: ... 0.5 ml of the heptane phase were mixed with ethanol in a 1:3 ratio (v/v) and the absorbance at 233 nm was measured on a SmartSpec Plus spectrophotometer (Bio-Rad) with extraction blanks used as references ...
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bioRxiv - Developmental Biology 2019Quote: ... and incubated with 1:200 rabbit-anti NF-B p65 polyclonal antibody (Pierce) (2 h) and 1:5000 Goat anti-rabbit IgG (H+L)-HRP conjugate (Bio-Rad) (1 h) ...
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bioRxiv - Cancer Biology 2020Quote: ... Aqueous droplets with a 20-µl volume containing final concentrations of 1× Droplet Digital PCR Supermix for probes (Bio-Rad Laboratories) and 100 ng of the genomic DNA template were generated ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The membranes were then incubated with HRP-conjugated secondary antibodies (1:5000, anti-Gt, #61-1620, Invitrogen; anti-Rb, #170-5046, Bio-Rad; or mouse IgG kappa binding protein ...
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bioRxiv - Molecular Biology 2019Quote: ... qPCRs were performed using 2ul of 1:100 diluted first PCRs as a template with qPCR primers (Table S4) and iTaq Universal SYBR Green Supermix (Bio-Rad) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2019Quote: ... A one in 10 dilution of cDNA was used for qPCR reaction mixture with final concentration of 1× SsoAdvanced universal SYBR super mix (Bio-Rad) and 1×PrimePCR assays for the designated target ...
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bioRxiv - Neuroscience 2021Quote: ... of each sample was loaded on an 8-16% (for TSPO, 3b-HSD, and StAR) or 4-20% (for mERα, caveolin-1, and PKA) SDS precast gels (Bio-Rad) and separated by electrophoresis ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies (Supplementary Table 1) overnight at 4°C and visualised with HRP-conjugated anti-IgG secondary antibodies and enhanced chemiluminescence substrates (Bio-Rad). The expression of PDHX or calnexin was used as a loading control.
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bioRxiv - Neuroscience 2021Quote: ... for 10 minutes followed by 15 minutes of tris-buffered saline with 0.25% Triton X-100 then blocked for 1 hour in tris-buffered saline with Tween 20 solution at 0.05% (BioRad #161-0781) supplemented with sterile 2% Normal Donkey serum (Abcam #ab138579 ...
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bioRxiv - Biophysics 2022Quote: ... 1 mg of post-thrombin digested claudin-4 in DDM was treated with 4 mg of amphipol (1:4 w/w) for 2 hours before removing detergent via addition of 400 mg SM-2 biobeads (Bio-Rad). Excess cCpE was added to each claudin-4 sample at a molar ratio of 1:1.5 ...
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bioRxiv - Biochemistry 2021Quote: ... The melting curves were measured at 1°C/min at the FRET channel in triplicate measurements and the data analyzed with CFX Manager™ (BioRad).
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bioRxiv - Biochemistry 2021Quote: ... The cells were replenished with compounds every 24 h and counted on days 1-9 using automated cell counter (Bio-Rad). KYSE-520 cells were plated onto 96-well plates (500 cells per well ...