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Citations for Bio-Rad :
9251 - 9281 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Equal amounts of proteins from lysates were separated by SDS-PAGE using Mini-PROTEAN® TGX™ 10% Precast Gels (Bio-Rad, Hercules, CA, USA) before transfer onto a Sequi-Blot PVDF membrane (Bio-Rad ...
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bioRxiv - Cancer Biology 2022Quote: ... and heated to 95°C for 10 minutes before being loaded into precast gels (Mini-PROTEAN® TGX™ Precast Protein Gels, BioRad, Cat. # 4561094, 4561096). The Precision Plus Protein™ Dual Color Standards (BioRad ...
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bioRxiv - Cell Biology 2019Quote: Reduced and heat-denatured protein extracts were separated by SDS-PAGE (4–20% Mini-4– 20% Mini-PROTEAN® TGX™ Gel, Bio-Rad, 456-1094) and then transferred onto 0.2 µm nitrocellulose membranes (Trans-Blot Turbo Transfer Pack ...
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bioRxiv - Molecular Biology 2020Quote: ... Supernatants were transferred to new tubes containing 87% glycerol (final concentration of 10%) and concentrations were adjusted using Bio-Rad protein assay (Bio-Rad, Hercules, California, United States).
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bioRxiv - Plant Biology 2021Quote: ... Protein concentrations were quantified using the Coomassie Blue dye binding method (Bradford, 1976) using a concentrated dye purchased from BioRad (500-0006, Hercules, CA, USA). AsA and DHA concentrations were determined by an ascorbate oxidase based method according to Lin et al ...
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bioRxiv - Neuroscience 2021Quote: ... or 40 μg (splenocytes of WT and Pik3cd−/− mice) of lysate were loaded onto 4-15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories B.V.). Transfer was performed onto Immobilon®-P PVDF Membranes (Merck KGaA) ...
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bioRxiv - Neuroscience 2020Quote: Immunoprecipitation of transfected COS-7 cells or C2C12 myotubes transduced with shVangl2 or shScr was performed as described previously (Strochlic et al., 2012) with SureBeads™ protein G magnetic beads (BioRad, catalog no. 161-4023).
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bioRxiv - Microbiology 2021Quote: ... The free RNA and RNA-protein complexes in gels were transferred onto a nylon membrane and cross-linked using GS Gene Linker™ UV Chamber (Bio-Rad Laboratories, Hercules, CA). The nylon membrane was incubated with 20 μg/mL of proteinase K (Ambion ...
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bioRxiv - Neuroscience 2022Quote: ... and protein standards were mixed with Laemmli buffer and loaded on 4-15% Criterion TGX Stain-Free protein gels in 1×□TGS running buffer (all from Bio-Rad, Marnes-la-Coquette, France) and transferred to a 0.20μm PVDF membrane with the Trans-Blot Turbo RTA Midi Transfer Kit (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: ... The samples were heated with Laemmli sample buffer with 2-mercaptoethanol at 95°C for 20 min and separated on SDS-PAGE using Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, USA).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Equal amounts of proteins were separated by SDS-PAGE and transferred to nitrocellulose membranes by using Trans-Blot Turbo™ Transfer Pack (Bio-Rad, Hercules, CA, USA). Western blot analysis was performed using primary antibodies against p-ERK (Cell Signaling Technologies Danvers ...
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bioRxiv - Neuroscience 2022Quote: For each lane, ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Microbiology 2023Quote: ... Aliquots of clarified lysate (3 x 106 parasite equivalents) were resolved by SDS-PAGE (Mini-PROTEAN®TGX protein gel; Bio-Rad, Hercules, California, USA), transferred to PVDF membrane (Immobilon®-FL ...
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bioRxiv - Molecular Biology 2023Quote: ... The secondary antibodies were detected via chemiluminescence using Westar Supernova (XLS3, 0020, Cyanogen) and the protein bands were visualized in a ChemiDoc XRS + imager (Bio-rad with image Lab software) (Ayagama et al. ...
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bioRxiv - Biochemistry 2023Quote: ... proteins were transferred to a nitrocellulose membrane for 55 min at 15V using a semi-dry electroblotting transfer unit (Bio-Rad, Hercules, CA, United States). Membranes were incubated for 1 hour with blocking buffer (1% dry skim milk ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were then transferred to the membranes by semi-dry Western blotting in a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories Inc., CA, USA). Next ...
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bioRxiv - Neuroscience 2024Quote: ... was boiled for 10 min at 97°C and loaded into 8% SDS-PAGE gels with Precision Plus Protein Standard (Bio-Rad, 161-0374/161-0375). Separated proteins were transferred to a nitrocellulose membrane (0.2 μm ...
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bioRxiv - Pathology 2019Quote: ... Samples were mixed with SDS-loading buffer without heating and electrophoretically separated on a 4–20% Criterion™ TGX™ Precast Midi Protein Gel (Bio-rad, cat. number 5671094) and transferred to nitrocellulose membrane ...
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bioRxiv - Neuroscience 2020Quote: ... 30 μg of protein was heated in a 70°C water bath for 10 min before loading into a 4 – 20% Criterion TGX precast Midi protein gel (Bio-Rad, Hercules, CA, USA; Cat# 5671094) for SDS-PAGE followed by wet transfer to a nitrocellulose membrane ...
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bioRxiv - Biochemistry 2021Quote: The proteins extracted from Ni-NTA resin were subjected to SDS-PAGE on 4–15% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad, Hercules, CA, USA) using the standard procedure ...
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bioRxiv - Microbiology 2023Quote: ... TCA precipitated protein mixtures were dissolved in 30 µL 1 x Laemmli sample buffer and loaded on Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories, Germany). After short 3 cm SDS PAGE separation ...
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bioRxiv - Microbiology 2023Quote: PVC syringes were purified via co-immunoprecipitation of one of the PVC proteins with a gravity flow column (Bio-Rad Econo-column®; cat. no.: 7371512), used according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... the membrane was incubated with HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at 25°C before washing it six more times ...
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bioRxiv - Genomics 2023Quote: ... Citrate synthase activity was standardized against the protein concentration of each sample using the Bovine Serum Albumin Assay (500-0201, Bio-Rad Laboratories, Inc., Hercules, CA, USA) and reported as nmol min−1 mg−1 protein after comparison with a glutathione (GSH ...
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bioRxiv - Cancer Biology 2022Quote: ... followed by separation on 10% SDS-PAGE and the separated proteins were electrotransferred onto polyvinylidene fluoride (PVDF) membraneusing a Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories, Inc., India). The membranes were first blocked with 5% non-fat milk in TBST for 1hr at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... The membrane was then washed thrice for 5 mins in PBS-Tween 20 before incubation with an HRP-conjugated anti-mouse secondary antibody (1:1000) and streptactin antibody (specific to the standard protein marker – Bio-Rad, Cat# 1610376; 1:1000 dilution) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... Volumes containing 10 µg of protein were resolved using SDS-PAGE (NuPAGE 3-12% gels, Life Technologies or 10% acrylamide Tris-Glycine, Bio-Rad for desmin fibrillar aggregates) and transferred to a nitrocellulose membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... following gel electrophoresis proteins were transferred from the gel to a polyvinylidene fluoride (PVDF) membrane using a Trans-Blot Turbo Transfer System (BioRad, Trans-Blot turbo RTA transfer kit) using the Bio-Rad standard protocol (25 V,30 min) ...
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bioRxiv - Cell Biology 2021Quote: ... Resulting protein extracts were subjected to 10 % (w/v) SDS-polyacrylamide gel electrophoresis and transferred to PVDF membranes (Bio-Rad, Hercules, CA, USA, 0.2 μm pore size). Upon incubation with 5 % (w/v ...
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bioRxiv - Cell Biology 2019Quote: ... were suspended in 100μL DPBS and 1 μL of each primary antibody against the following proteins: FOXP3-PE (eBioscience, San Diego, CA, USA; 1:100) and CD3-FITC (MCA1774F; Bio-Rad, San Diego, CA, USA; 1:100). After incubation for 1 h at 23 ± 2 °C ...
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bioRxiv - Bioengineering 2023Quote: ... To confirm PEGylation the product and reactants were analyzed by SDS-PAGE using Mini-PROTEAN® TGX™ Precast protein gels (Bio-Rad Laboratories Inc., CA, USA).