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Citations for Bio-Rad :
8901 - 8950 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... Protein concentration of the supernatant was determined using the BCA protein assay (Pierce™) and 15 µg was loaded on the gel (3450124, Bio-rad), which was next turbo transferred on nitrocellulose membrane (1704159 ...
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bioRxiv - Immunology 2022Quote: ... Peak fractions pooled and the concentrations of conjugated mi3 particles were determined using a Bio-Rad Protein Assay (Bio-Rad, Hercules, CA). Conjugated nanoparticles were characterized by electron microscopy imaging and SEC as shown in Figure S1C-E ...
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bioRxiv - Molecular Biology 2022Quote: ... Either 50 μg or 25 μg (puromycin blots only) of total protein was separated by SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620177) for blotting ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies used in this study were anti-myelin basic protein (MBP, rat, monoclonal, clone 12, Bio-Rad, cat# MCA409S, 1:1000), anti-Neurofilament 200 (NF200 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... the whole cell membrane pellets were resuspended in buffer and protein concentration was estimated by dye binding (Coomassie Brilliant Blue R-250; Bio-Rad, USA). The binding reaction was carried out in a final volume of 0.1 ml of Na+-containing buffer (20 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2023Quote: ... Equal amounts of protein extract (20–30 μg) were resolved by SDS-PAGE (4–15% gradient precast TGX polyacrylamide gel, Bio-Rad Laboratories), followed by standard western blot procedure ...
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bioRxiv - Molecular Biology 2023Quote: ... The high molecular weight protein GCN1 was transferred using the Trans-Blot Turbo Transfer System (Bio-Rad, Cat# 1704150 and Cat# 1704158). Subsequently ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins (30 μg/lane) were separated on an SDS-polyacrylamide gel and transferred to a polyvinylidene difluoride (PVDF) membrane (Bio-Rad Laboratories). The membrane was immunoblotted for CSF1R (#MAB3291 ...
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bioRxiv - Neuroscience 2022Quote: ... Protein samples (∼15 μg) were loaded and separated on 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE, Bio-Rad, Hercules, CA), and transferred to polyvinilidene difluoride membranes (PVDF ...
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bioRxiv - Microbiology 2022Quote: ... Laemmli buffer was added to samples.10 μL of samples were loaded onto 12 % Mini-P ROTEAN® TGX™ Precast Protein Gels (Biorad). Silver staining was carried out at room temperature for 1 h and gels were developed for 2 to 3 mins ...
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bioRxiv - Neuroscience 2022Quote: ... ∼20 μg protein were run on a 4-12Bis-Tris Criterion XT Precast Gel (#3450123, Bio-Rad (Bio-Rad, Hercules, CA, USA) next to the Precision Plus Protein Dual Color Standard (catalog#161-0374 ...
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bioRxiv - Physiology 2022Quote: ... Proteins were visualized by the enhanced chemiluminescence technique (ECL, Amersham, Munich, Germany) and developed using ChemiDoc™ Imaging System (BioRad, Feldkirchen, Germany).
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bioRxiv - Biophysics 2022Quote: ... Samples were centrifuged for 2 min at 12,000 x g before being separated on 8–16% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad) at 160 V for 60 min ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total protein amount of 100 μg for each sample was separated on 12% Criterion gels (Bio-Rad Laboratories, Hercules, Ca, USA), following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Protein lysates were boiled in loading buffer [1:9 ratio of 2-mercaptoethanol:4x Laemmli Sample Buffer (Cat.#1610747; Bio-Rad Laboratories)] for 10 min ...
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bioRxiv - Microbiology 2024Quote: ... SigA proteins were detected using anti-SigA85 (1:10,000 dilution) combined with anti-rabbit-HRP secondary (Bio-Rad, #1706515, 1:5,000 dilution) antibodies ...
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bioRxiv - Physiology 2024Quote: ... An equal mass of protein was separated via polyacrylamide gel electrophoresis using Criterion TGX Stain-Free midi gels (Biorad 5678084 or 5678085) or NuPAGE Bis-Tris gels (ThermoFisher ...
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bioRxiv - Genetics 2024Quote: ... The pulled down proteins were separated by Sodium dodecyl sulfate Poly-Acrylamide Gel Electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride membranes (Bio-Rad Laboratories). Membranes were incubated with anti-ACTB (Proteintech ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein pellet was resuspended in UTC buffer (8 M urea, 2 M thiourea, 4% CHAPS) and quantified using RC DC™ Protein Assay Kit (Bio-Rad). Sample with 80 µg proteins resuspended in rehydration buffer (8 M urea ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein(s) in the gel were transferred to a PVDF membrane using a Mini-PROTEAN Tetra Cell (Bio-Rad, United States). After blocking the membrane with 3% BSA at 4°C overnight ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of proteins (30µg) were separated by 7.5% SDS-PAGE and transferred to nitrocellulose membranes (Bio-Rad, Marnes-la-Coquette, France). After blocking membranes in Tris Buffer Solution with Tween-20 0.1 M (TBST ...
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bioRxiv - Neuroscience 2024Quote: ... protein was diluted to an equivalent concentration for each sample in additional 1% SDS with 4X Laemmli sample buffer (Bio-Rad, #1610747) and 2-mercaptoethanol ...
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bioRxiv - Neuroscience 2024Quote: ... Equal quantities of proteins from each individual sample were separated on mini-Protean® TGX™ mini pre-cast gels (BioRad, #4561023) at 120V until the leading dye reached the bottom of the gel ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by size by running 20 μg of the protein extract in 8% SDS-PAGE and transferred to Immuno-Blot PVDF Membrane (Bio-Rad, 1620174) using the Trans-Blot® SD Semi-Dry Transfer Cell (Bio-Rad ...
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bioRxiv - Microbiology 2024Quote: ... elution fractions with the highest protein concentrations were pooled and checked by SDS-PAGE (Laemmli, 1970) using a 4–20% Mini-PROTEAN gradient gel (BioRad, Munich, Germany). Final protein concentration of the pooled elution fraction was determined with the Pierce BCA Protein Assay Kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Proteins were separated by adding 20 μg of protein lysate to 4-20% gradient Mini Protean TGX Stain Free Pre-Cast Gels (Bio-Rad Laboratories). Proteins were then transferred to a nitrocellulose membrane (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: 40 μg of protein lysate was resolved on NuPAGE Novex 4-12 % Bis-Tris gels and transferred onto nitrocellulose membranes (Bio-Rad system). Membranes were blocked with 5 % BSA in TBS-T (10 mM Tris pH 8.0 ...
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bioRxiv - Neuroscience 2023Quote: ... Cell lysates were separated on SDS/PAGE and proteins on the gels were transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). The membranes were blocked with 5% skim milk diluted in TBST for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2023Quote: ... Protein lysates were resolved by SDS/polyacrylamide gel electrophoresis (PAGE) and transferred to polyvinylidene fluoride (PVDF) membranes (Bio-Rad, Hercules, CA, USA). After blocking ...
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bioRxiv - Cell Biology 2024Quote: ... The proteins were detected using chemiluminescence using horseradish peroxidase–conjugated secondary antibodies of anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) origin and enhanced chemiluminescence substrate (Thermo Scientific # 32106) ...
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bioRxiv - Developmental Biology 2024Quote: ... Protein samples were boiled for 10 minutes at 95 °C and loaded onto Mini-PROTEAN TGX™ Precast Gels (BIO-RAD:4569036), and electrophoresis was performed according to manufacturer’s guidelines ...
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bioRxiv - Immunology 2024Quote: ... SDS-PAGE was performed using 10 μg of NP-40 extract protein mixed with an equal volume of 2X Laemmli buffer (BioRad cat#: 1610737) supplemented with 5% 2-mercaptoethanol (without boiling) ...
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bioRxiv - Cell Biology 2024Quote: ... Transfer of the proteins from gel to the Immun-Blot® Low Fluorescence PVDF membrane with low fluorescent transfer buffer (BIORAD; #1704275) was performed in Trans-Blot Turbo Transfer System (BIORAD ...
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bioRxiv - Cancer Biology 2024Quote: ... Gel electrophoresis was performed on 4–20% Mini-PROTEAN® TGX™ Precast Protein Gels (Bio-Rad Laboratories AG, Cat#4561094/4561096). Proteins were transferred to Trans-Blot Turbo 0.2 µm Nitrocellulose Transfer Membrane (Bio-Rad ...
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bioRxiv - Biochemistry 2024Quote: ... The presence of the complex was assessed by 4-15% gradient SDS-PAGE using Mini-PROTEAN® TGX™ Precast Protein gels (BIORAD). Concentration of the RNAP in these fractions was determined by absorbance at 280 nm using NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer (Thermofisher ...
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bioRxiv - Biophysics 2024Quote: ... The pellet was then resuspended in binding buffer (50mM Tris, 120 mM NaCl, pH 7.4) Bradford protein assay (Bio-Rad, Hercules, CA) was used to determine the protein concentration and the membranes were diluted to 1000 μg/mL and stored in a −80 °C freezer for later use ...
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bioRxiv - Cell Biology 2024Quote: ... The protein concentrations of cell lysates were measured using BCA assay (Thermo 23225) and equal amounts of proteins were loaded onto 4 – 15 % polyacrylamide gradient gels (Bio-Rad 4561084) and fractionated by SDS-polyacrylamide gel electrophoresis (SDS-PAGE) ...
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bioRxiv - Neuroscience 2023Quote: ... The eluted protein was subjected to an additional purification step using fast protein liquid chromatography (FPLC) and a Superdex 200 Increase 10/300 GL column (Cytiva, 28990944) in Chelex 100-treated (Bio-Rad, 1422832) dialysis buffer (25 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... A normalized volume equivalent to 20 μg total protein in the predigested sample was resolved on a 4-12% Criterion XT Bis-Tris gel (Bio-Rad 3450124) at 100V for 2 hours ...
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bioRxiv - Immunology 2023Quote: ... A total of 30 µg of protein per well was loaded onto a 4-15% precast SDS-PAGE gel (Bio-Rad, 4561083). SDS-PAGE running conditions are as follows ...
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bioRxiv - Microbiology 2023Quote: ... Protein samples were fractionated by SDS-polyacrylamide gel electrophoresis using a 4%–20% Mini-PRO TEAN TGX Gel (BioRad, Hercules CA, USA), and the resolved proteins were transferred by electroblotting onto polyvinylidene difluoride membranes (Immobilon-P PVDF Transfer Membranes) ...
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bioRxiv - Microbiology 2023Quote: ... Lysates were then vortexed for 1 min at max speed and loaded into a 10% sodium dodecyl sulfate (SDS)-polyacrylamide gel and run at constant 100 V for 1 h 45 min in running buffer (25 mM Tris, 190 mM Glycine, 3.46 mM SDS) alongside a Precision Plus Protein Dual Color Standard (Bio-Rad, Cat #1610374) in ...
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bioRxiv - Neuroscience 2022Quote: ... extracted proteins were first immobilized and resolved based on molecular weight on 4–15% Mini-PROTEAN TGX Precast gels (Bio-Rad Laboratories) through sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) ...
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bioRxiv - Developmental Biology 2023Quote: 30 to 60 µg of protein obtained from AT2 were separated on 4-20% Criterion TGX Gels (Bio-Rad. Cat. No. 5678093), transferred to PVDF membranes (Trans-Blot Turbo System ...
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bioRxiv - Neuroscience 2023Quote: ... A 150 µl aliquot of this crude homogenate was removed and protein concentration was determined using the Bradford method (BioRad, Hercules, CA). Subcellular fractionation by density gradient ultracentrifugation using Optiprep was performed on remaining 600 µl sample for the 6- and 12-month-old mice as previously described 47.
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bioRxiv - Neuroscience 2023Quote: ... Once ready for testing we quantified the amount of mitochondria from each isolation using Bio-Rad Protein Assay Kit (Bio-Rad, #5000001), plated 3µg/50µl/well onto a 96 well plate with all animals having 6-8 technical replicates (101085-004 ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and stored at -80 until α5 subunit and its binding partners were immunoprecipitated using SureBeads protein G magnetic beads according to the manufacturer’s protocol (Biorad, Hercules, CA, USA). Western blots (detailed in section 2.8 ...
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bioRxiv - Biochemistry 2023Quote: ... and 30 μg of the proteins were loaded on 4-12% Criterion TGX Stain-Free precast polyacrylamide gels (BIO-RAD, Cat# 5678084) and transferred to nitrocellulose membranes which were subsequently blocked by 5% BSA for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...
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ALS iPSC-derived microglia and motor neurons respond to astrocyte-targeted IL-10 and CCL2 modulationbioRxiv - Neuroscience 2023Quote: ... Equal amounts of protein were loaded onto 10% or 12% pre-cast Tris-HCl Mini-PROTEAN gels (Bio-Rad, Hercules, CA, USA) and proteins separated by electrophoresis ...