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Citations for Bio-Rad :
8951 - 9000 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... A 150 µl aliquot of this crude homogenate was removed and protein concentration was determined using the Bradford method (BioRad, Hercules, CA). Subcellular fractionation by density gradient ultracentrifugation using Optiprep was performed on remaining 600 µl sample for the 6- and 12-month-old mice as previously described 47.
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bioRxiv - Developmental Biology 2023Quote: 30 to 60 µg of protein obtained from AT2 were separated on 4-20% Criterion TGX Gels (Bio-Rad. Cat. No. 5678093), transferred to PVDF membranes (Trans-Blot Turbo System ...
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bioRxiv - Neuroscience 2023Quote: ... The proteins in the gel were transferred to a nitrocellulose membrane by Trans-Blot® Turbo™ Transfer System (Bio-Rad Laboratories) at 25 V ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and stored at -80 until α5 subunit and its binding partners were immunoprecipitated using SureBeads protein G magnetic beads according to the manufacturer’s protocol (Biorad, Hercules, CA, USA). Western blots (detailed in section 2.8 ...
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bioRxiv - Cell Biology 2023Quote: ... Equal protein loading was confirmed using a stain-free visualization system (Criterion TGX Stain-Free Precast Gels, BioRad Laboratories, Hatfield, PA, USA) and the protein transferred to 0.45 mm polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2023Quote: ... The determination of casein concentration by the Lowry method was performed using the DC™ protein assay kit purchased from Bio-Rad Laboratories (California ...
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bioRxiv - Biochemistry 2023Quote: ... GFP-Cdc42p and ubiquitin were detected under non-saturated conditions and normalized to the housekeeping protein Pgk1p using the Image Lab Software (Bio-Rad, Inc.). Wild-type cells and control conditions were set to a value of one and adjusted for other samples accordingly.
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bioRxiv - Bioengineering 2022Quote: ... Fifteen micrograms total protein extracts were separated by 4–20 % stain-Free SDS-PAGE gels and transferred onto polyvinylidene difluoride (PVDF) membranes (Bio-Rad Laboratories). Membranes were blocked using Tris-buffered saline (TBS ...
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bioRxiv - Biochemistry 2022Quote: ... Gel demonstrating protein purity was generated using by loading 1 μg of each protein onto a Mini-PROTEAN TGX 4-20% gradient gel (BIO-RAD, 4561096) and then staining with Coomassie blue.
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bioRxiv - Cell Biology 2022Quote: ... The proteins were detected using chemiluminescence using HRP conjugated secondary antibodies of either anti-mouse (Bio-Rad # 170-6516, dilution 1:10,000) or anti-rabbit (GE Healthcare # NA9340 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 1 μg/μL of total cellular protein from each sample was used to run a PAGE and transferred onto a PVDF membrane (Bio-Rad, 1704157). The membranes were soaked in a blocking solution of 5% BSA (AMRESCO ...
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bioRxiv - Biophysics 2023Quote: ... an aliquot of 25µg of total protein per sample was loaded into 4–20% Mini-PROTEAN® TGX™ precast gels (Bio-Rad) for SDS-PAGE separation ...
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bioRxiv - Genetics 2023Quote: ... and equal amounts of protein sample were then loaded onto a 4-20% gradient SDS-PAGE gel (Bio-Rad, cat no. 4561095) and run at 120V before being transferred to a PVDF membrane (Bio-Rad ...
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bioRxiv - Evolutionary Biology 2023Quote: ... purified protein fractions were separated with 10%–20% SDS-PAGE and transferred onto polyvinylidene difluoride (PVDF) membrane (Bio-Rad, Hercules, CA, USA) using a semi-dry blotter (ATTO ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatants of the cell lysates were normalized for total protein concentration according to Bradford assay (#5000006, Bio-Rad, Hercules, CA, USA), mixed with SDS loading dye ...
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bioRxiv - Cell Biology 2023Quote: ... The protein concentration of the supernatant was determined by Bradford measurement (64) according to the manufacturer’s instructions (Bio-Rad, cat n° 5000006).
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bioRxiv - Neuroscience 2023Quote: ... Supernatants were collected and 10 µl of protein sample was loaded in each well of precast Mini-PROTEAN TGX 4-20% gradient Gel (BIO-RAD 4561096). Following SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: Proteins were resolved by SDS-PAGE using 4-20 % or 4-15% TRIS-glycine gradient midi gels (Bio-Rad, Hercules, CA, USA), in electrode buffer (0.1% w/v SDS ...
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bioRxiv - Neuroscience 2023Quote: ... 50 ug of protein was diluted with Laemmli buffer and loaded without heat denaturation onto a Criterion 10-20% tris-HCl gel (Bio-Rad, 3450043) run in tris-glycine SDS buffer ...
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bioRxiv - Bioengineering 2023Quote: ... 15 ug protein were loaded and separated using 4–15% Mini-PROTEAN® TGX™ Precast SDS-PAGE Gels (Bio-rad, #4561086). Samples were then transferred to nitrocellulose membranes for immunoblotting ...
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bioRxiv - Bioengineering 2023Quote: ... All samples with equal amounts of protein were loaded on gels and separated by reducing SDS-PAGE and transferred to PVDF membranes (Bio-Rad, US). Membranes were blocked with 5% milk powder and incubated with rabbit anti-human Sox2 (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... the supernatant in a new tube received 20% glycerol and protein concentration was estimated by Bradford’s Dye Reagent Concentrate (BioRad Laboratories, Hercules, CA). For gel electrophoresis ...
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bioRxiv - Plant Biology 2023Quote: ... and proteins were separated in SDS/urea–PAGE (15% polyacrylamide, 6 M urea) (Laemmli, 1970) using mini-protean apparatus (Bio-Rad USA). These separated proteins were transferred to a nitrocellulose membrane using trans-blot apparatus (Bio-Rad USA) ...
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bioRxiv - Cancer Biology 2023Quote: ... 20 mg of whole cell extract or 40-80 mg of liver homogenate was loaded on Mini-Protean TGX protein gel (4-20% or 7.5%) (Bio-Rad, Hercules, CA) and transferred to Immobilon-P membranes by electrophoresis (MilliporeSigma ...
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bioRxiv - Cancer Biology 2023Quote: ... were loaded on 10 % or 4–20 % Mini-PROTEANR TGX™ Precast Protein Gels and separated at 100 V in Tris/Glycine/SDS buffer in Mini-Protean Tetra System (Bio-Rad). The proteins were transferred to a polyvinylidene difluoride membrane (PVDF ...
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bioRxiv - Neuroscience 2023Quote: ... Once ready for testing we quantified the amount of mitochondria from each isolation using Bio-Rad Protein Assay Kit (Bio-Rad, #5000001), plated 3µg/50µl/well onto a 96 well plate with all animals having 6-8 technical replicates (101085-004 ...
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bioRxiv - Cell Biology 2023Quote: 600 μL of DMEM GlutaMax™ supplemented with 10% FBS, 20 μg/mL gentamycin, and either 100 ng/mL recombinant mouse Monocyte chemoattractant protein-1 (MCP-1, CCL2) (Bio-Rad, PMP35) or vehicle control (ddH2O) ...
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bioRxiv - Neuroscience 2023Quote: ... We loaded 50 μg of reduced protein per gel lane and performed transfer with Trans-Blot® TurboTM Transfer System (Bio-Rad) at 25 V for 7 min ...
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bioRxiv - Physiology 2024Quote: ... An equal mass of protein was separated via polyacrylamide gel electrophoresis using Criterion TGX Stain-Free midi gels (Biorad 5678084 or 5678085) or NuPAGE Bis-Tris gels (ThermoFisher ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were prepared for gel electrophoresis by adding a volume containing 20 ug protein to 3 uL 1 M DTT and 7.5 μL 4X Laemmli sample buffer (BioRad Cat. No. 1610747) and topping up to 30 μL with deionized water ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: A total protein amount of 100 μg for each sample was separated on 12% Criterion gels (Bio-Rad Laboratories, Hercules, Ca, USA), following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... Gels were loaded with samples containing 10 μg total protein before electrophoresis on a 16.5% Tris Tricine gel (Bio-Rad, Hercules, CA). Gels were stained with the Pierce Silver Stain kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2023Quote: ... Protein concentration was determined using a colorimetric assay based on the Bradford dye-binding method (Bio-Rad Protein Assay Dye Reagent, Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of proteins were separated on 8% reducing SDS-PAGE and transferred to Nitrocellulose membranes (Ref# 162-0112, BIO-RAD, Germany). Primary antibodies anti-SERCA2a and anti-SERCA2b targeted the specific ends of SERCA2a and SERCA2b (see supplement) ...
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bioRxiv - Cell Biology 2023Quote: ... Between 20-30 µg of protein was electrophoresed in 4-15% miniPROTEAN TGX Stain-Free pre-cast gels (BioRad, 4568084/4568085/4568086) using electrophoresis buffer [24.76 mM Tris Base (American Bio AB02000-05000) ...
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bioRxiv - Cancer Biology 2023Quote: ... Equal amounts of protein samples (15 μg/lane) were subjected to analysis by 10% SDS-PAGE and transferred to a PVDF membrane (Bio-Rad, 1620264) using either semi-dry or wet electroblotting (for proteins with a molecular weight of 100 kDa and above) ...
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bioRxiv - Microbiology 2024Quote: ... SigA proteins were detected using anti-SigA85 (1:10,000 dilution) combined with anti-rabbit-HRP secondary (Bio-Rad, #1706515, 1:5,000 dilution) antibodies ...
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bioRxiv - Molecular Biology 2023Quote: The interaction between nucleic acids (ligands) and the proteins (analytes) were measured by the surface plasmon resonance (SPR) technique using a ProteOn XPR36TM (Bio-Rad Laboratories). NLC Sensor chips precoated with NeutrAvidin (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2023Quote: ... extracellular proteins were separated on SDS 15% (m/v) polyacrylamide gels and electroblotted to Immun-Blot polyvinylidene difluoride membranes (Bio-Rad, USA) using a Mini Trans-Blot electrophoretic transfer cell (Bio-Rad) ...
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The HUSH epigenetic repressor complex silences PML nuclear bodies-associated HSV-1 quiescent genomesbioRxiv - Microbiology 2024Quote: ... Protein extracts were boiled for 10 minutes and then loaded on polyacrylamide gels (TGX Stain-Free FastCast gels (Bio-Rad, 1610181/1610183) or home-made gels ...
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bioRxiv - Physiology 2024Quote: ... Proteins were separated by SDS-PAGE and transferred to PVDF membranes using a semi-dry transfer cell (Bio-Rad, Hercules, CA, USA). Membranes were blocked with 5% non-fat dry milk in TBS-Tween for 1 h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... The indicated total protein amount was loaded on SDS-PAGE gels (4-20% Mini-protean® TGX™ precast gels, [Bio-Rad] ...
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bioRxiv - Immunology 2024Quote: ... A total of 80 µg of protein homogenate were separated by SDS-PAGE and transferred onto PVDF membranes (Bio-Rad, Hercules, CA). Membranes were blocked with fat-free milk powder (5% w/v ...
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bioRxiv - Cell Biology 2024Quote: ... After running the samples by an SDS polyacrylamide gradient gel (4–20% Criterion™ TGX™ Precast Midi Protein Gel, Bio-Rad) and transferring them to a PVDF membrane (350mA/1h) ...
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bioRxiv - Plant Biology 2024Quote: ... the immunoprecipitated and input proteins were separated on SDS–PAGE gels and transferred onto PVDF membranes via a Trans-Blot Turbo Transfer System (Bio-Rad, USA). After blocking the membranes with a solution of 5% skim milk prepared in PBST with 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2024Quote: ... Protein was resolved using SDS-PAGE and transferred to the nitrocellulose membrane using a transfer system (Trans-Blot Turbo; Bio-Rad Laboratories). The membrane was blocked using 5% skimmed milk (American Bioanalytical ...
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bioRxiv - Microbiology 2024Quote: ... Membranes were washed as before and protein bands visualised using the enhanced chemiluminescent substrate (ECL) with the ChemiDoc™ XRS system (BioRad, USA). Details of primary and secondary antibodies used are in Table 2.
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bioRxiv - Cancer Biology 2024Quote: ... A molecular weight marker was added onto each gel for reference (Precision Plus Protein Dual Color, #1610374, Bio-Rad, Hercules, CA, USA). Transfer on PVDF membranes was done using Pierce Western blot transfer buffer (#35040 ...
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bioRxiv - Cell Biology 2024Quote: ... The stain-free gel was UV-activated for 1 minute and imaged for total protein (BioRad Chemi Doc XR+, Hercules, CA, USA) with ImageLab software (BioRad Image Lab v6) ...
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bioRxiv - Cell Biology 2024Quote: ... Proteins were transferred onto Trans-Blot® Turbo™ Mini PVDF Transfer membranes with the Trans-Blot Turbo Transfer System (Bio-Rad). Membranes were blocked with 5% skimmed milk in PBS with added Tween20 0.05% (Merck ...