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Citations for Bio-Rad :
9151 - 9200 of 9281 citations for Nuclear Protein Extraction since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The recombinant protein was transferred to 0.45 μm nitrocellulose membranes using the Trans-Blot® Semi-Dry Transfer Cell (Bio-Rad, CA, USA), and bands were confirmed by 0.1% Amido black staining solution (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... and the protein bands were visualized with an enhanced chemiluminescence (ECL) solution (Clarity Western ECL Substrate, 1705062 Bio-Rad Labs, Des Plaines, IL). Quantitative analysis of the bands was performed with ImageJ software (version 1.49 ...
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bioRxiv - Cancer Biology 2021Quote: ... Proteins were immunoprobed using an HRP-conjugated secondary antibody (dilution 1:3000) and chemiluminescent substrate (Clarity Western ECL substrate, Bio-Rad, Hercules, CA, USA). Blot image acquisition was performed using a Molecular Imager® Gel Doc™ XR+ system (Bio-Rad ...
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bioRxiv - Developmental Biology 2020Quote: ... 5μg of either whole brain lysate or tubulin-enriched protein fraction was loaded per lane and run on 4-20% Mini-Protean TGX Stain-Free precast gels (4568093; Bio-Rad Laboratories, Hercules, CA) at 150mV for 1hr ...
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bioRxiv - Molecular Biology 2020Quote: ... Reducing SDS sample buffer was added and proteins resolved on 4%-15% Mini-PROTEAN TGX Stain-Free Precast Gels (Bio-Rad, California, United States) or 3%-8% NuPAGE Tris-Acetate Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... 30 µg of WCE were separated on mini protein TGX stain-free gel and electroblotted using the PVDF transfer membrane (Bio-Rad, Hercules, CA, USA). The ChemiDoc™ MP imaging system (Bio-Rad ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 μl of the resulting solution was then applied to either 4-12% Criterion™ XT Bis-Tris Protein Gels (BioRad, Hercules, CA, USA) or NuPAGE™ 4 – 12% Bis-Tris Mini Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: ... The protein levels were quantified with ImageLab software after acquisition of chemiluminescent signals with a Chemidoc digital imager (Bio-Rad, Marnes-la-Coquette, France). For all SDS-PAGE analyses ...
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bioRxiv - Physiology 2019Quote: ... The remaining supernatant was diluted to 1.5 µg × µl−1^ total protein in lysis buffer and 4X Laemmli sample buffer (Bio-Rad Laboratories AB, Oslo Norway) containing 2-Mercaptoethanol ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... The triglyceride content was normalized to the total protein content of the flies estimated by Quick Start™ Bradford 1X Dye Reagent (BioRad, Cat. # 500-0205). Freshly emerged flies feed less and possess larval triglyceride energy storage ...
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bioRxiv - Cancer Biology 2019Quote: ... 20 μg proteins of the cell line were exposed to a 10% SDS-PAGE gel and transferred to nitrocellulose membranes (Bio-Rad, Hercules, CA, USA). After blocking the membranes using 5% non-fat milk or bovine serum albumin (BSA ...
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bioRxiv - Physiology 2020Quote: ... and equal amounts of total protein (15 or 20 μg) were loaded in different wells on Criterion™ 4-20% TGX Stain-Free™ Precast Gels (Bio-Rad, Australia). A stain-free system (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... and purity assessed by 15% SDS-PAGE using Bio Rad Precision Plus Protein All Blue Standards (10-250 kDa) as molecular weight ladder (Bio-Rad; Hercules, CA, USA).
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bioRxiv - Neuroscience 2021Quote: ... Target proteins were detected by chemiluminescence with Clarity™ (Beta-actin and GFP) and Clarity Max™ (SP) Western ECL Substrate kit (Bio-Rad®) on the Odyssey FC system (LI-COR) ...
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bioRxiv - Microbiology 2021Quote: ... Cytokine levels within the lung homogenates of infected mice were analyzed using the Bio-Plex protein array system (Luminex-based technology, Bio-Rad Laboratories, Hercules, CA). Briefly ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were denatured for 5 min at 95°C and gel was run using a 4-20% Mini-PROTEAN ® TGX precast protein gels (Bio-Rad, No. 4561094). Next ...
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bioRxiv - Plant Biology 2020Quote: ... the recombinant protein in the filtrate was further purified by size exclusion chromatography (NGC™ chromatography system with Enrich™ SEC 650 column, BioRad, Munich, Germany) in 50 mM NaH2PO4 /Na2HPO4 ...
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bioRxiv - Plant Biology 2020Quote: ... Proteins extracts were boiled at 95°C and then separated onto Mini-PROTEAN ® TGX™ precast protein SDS-PAGE (4-15% gradient) gels (BIO-RAD, #4561083). Proteins were transferred onto polyvinylidene fluoride (PVDF ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary antibodies were detected via chemiluminescence using Westar Supernova (XLS3,0020, Cyanogen) and the protein bands were visualised in a ChemiDoc XRS+ imager (Bio-rad with image Lab software).
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bioRxiv - Cancer Biology 2021Quote: Westerns were performed by loading 30-50ug of total protein per well into 15 well polyacrylamide gels (Bio-Rad, California, USA, Cat #456-1086) and run for 30min at 200V ...
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bioRxiv - Cancer Biology 2021Quote: Separation of 20 μg or 50 μg protein from cell lysate was performed on a 10% Mini-PROTEAN® TGX™ gel (BioRad, Portland, ME) and subsequently transferred onto Immunoblot PVDF 0.45μm membranes (BioRad ...
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bioRxiv - Cell Biology 2021Quote: ... Fluorescent dye-conjugated secondary antibodies were used at 1:3000 and the proteins were detected using ChemiDoc MP Imaging System (Bio-Rad, Hercules, CA, USA).
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bioRxiv - Microbiology 2022Quote: ... Resulting lipopolysaccharides were separated using gradient 4–20% sodium dodecyl sulfate□polyacrylamide gel (4–20% Mini□PROTEAN® TGX™ Precast Protein Gel, BioRad, Hercules, USA) electrophoresis (SDS□PAGE ...
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bioRxiv - Bioengineering 2019Quote: ... Western blot analysis was performed using 30 to 50 µg proteins run on Mini-PROTEAN TGX precast gels (Bio-Rad cat. nr. 456-1094). αRANBP9 primary antibody was purchased from Abcam (cat ...
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bioRxiv - Neuroscience 2021Quote: ... to a final concentration of 10 mM and shielded from light for 30 minutes.We performed an automated detergent compatible protein assay (DCA) (Bio-Rad, Hercules, CA, Cat. #5000111), normalization (to 1 μg/μL ...
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bioRxiv - Microbiology 2021Quote: ... The native molecular mass of 12β-HSDH was determined by comparing its elution volume to that of Gel Filtration Standard proteins (Bio-Rad, Hercules, CA, USA): thyroglobulin ...
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bioRxiv - Bioengineering 2021Quote: Ramos B cells were retrovirally transduced with a construct encoding spike protein tagged with the fluorophore mScarlet at the C-terminus and sorted by FACS (Bio-Rad S3e Cell Sorter). For the experiment ...
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ALiCE®: A versatile, high yielding and scalable eukaryotic cell-free protein synthesis (CFPS) systembioRxiv - Biochemistry 2022Quote: Proteins were transferred from gels to PVDF membranes using a Trans-Blot® Turbo™ Transfer System and associated accessories (Bio-Rad Laboratories, Inc.). Membranes were probed with anti-Strep-II tag and anti-CB2 antibodies according to manufacturer instructions.
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bioRxiv - Plant Biology 2019Quote: ... equal protein amounts were loaded onto 7.5% polyacrylamide gels (Mini-PROTEAN®TGX Stain-Free™ Precast Gels, Bio-Rad Laboratories, Hercules, CA, USA) and fractionated by gel electrophoresis under reducing conditions and then blotted onto PVDF membranes (Immun-Blot®PVDF Membrane ...
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bioRxiv - Microbiology 2020Quote: ... as per the manufacturer’s running conditions then activated for 5 min under UV to determine total protein (Bio-Rad ChemiDoc XR, Image Lab Software). Proteins were transferred to PVDF membranes (Bio-Rad) ...
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bioRxiv - Cancer Biology 2019Quote: ... 50ug protein was separated on 10% ECL gels (GE, Pittsburgh, PA) and transferred to nitrocellulose using the Trans-Blot® System (Bio-Rad, Hercules, CA). Antibodies are listed in Supplemental Table S10.
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bioRxiv - Bioengineering 2019Quote: ... the protein bands were visualized upon few minutes of incubation of the membranes with Bio-Rad Clarity Western ECL Substrate (Bio-Rad#1705061, Cressier, Switzerland). Blots were imaged using ChemiDoc imaging system for chemiluminescence assays with 0.1 s exposure ...
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bioRxiv - Immunology 2020Quote: ... A chemiluminescence image of the ABCC4 blot was taken in tandem with an image of total protein loading using Bio-Rad Image Lab software (Bio-Rad Laboratories, Mississauga, Canada).
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bioRxiv - Synthetic Biology 2021Quote: ... diluted 1:200 in 10 mM Tris-HCl pH 7.5 and 9 μL loaded (along with 1 μL 1 M DTT and 10 μL 2X Laemmli Buffer) into an Any kD™ Mini-PROTEAN® TGX™ Precast Protein Gel (Bio-rad). For Western blot ...
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bioRxiv - Plant Biology 2021Quote: ... Protein quantities were measured by Bradford assays according to the manual provided with the Bio-Rad Protein Assay Dye Reagent Concentrate (Bio-Rad Laboratories; Hercules, USA). Proteins were immunoprecipitated by 50 µl Anti-HA MicroBeads (Miltenyi Biotec ...
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bioRxiv - Neuroscience 2021Quote: ... total protein was extracted and 15-20µg from each sample was separated on a 10% Mini-PROTEAN® TGX™ Precast Protein Gel (Biorad, Hercules, CA., USA) adjacent to the Li-COR Chameleon ladder (Li-Cor ...
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bioRxiv - Genetics 2020Quote: ... diluted samples were boiled for 5min at 95 °C and subsequently separated on a 4-15% Criterion™ TGX Stain-Free™ Protein Gel (Bio-Rad Laboratories) and ran in a Criterion™ Cell geltank (Bio-Rad Laboratories ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted proteins or whole cell lysates were separated by SDS-polyacrylamide gel electrophoresis on a 4-20% gradient gel (Bio-Rad Laboratories, Hercules, CA) and transferred to polyvinylidene fluoride (PVDF ...
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bioRxiv - Biochemistry 2021Quote: ... Protein extracts (50 μg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Biochemistry 2021Quote: 500 ng for each recombinant SARS-CoV-2 protein was prepared for electrophoresis using 4x Laemmli Sample Buffer (#1610747, Biorad Laboratories, Hercules, CA, USA) and incubated for 5 minutes at 95°C and then separated by 12% SDS-PAGE ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 µg of each protein mixture was separated by SDS-PAGE (polyacrylamide gel electrophoresis) on a Criterion XT 4-12% Bis-Tris gel (Biorad Laboratories cat. No. 3450124). Proteins were transferred to a PVDF membrane (Merck Millipore cat ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... following which they were subjected to separation via sodium dodecyl sulfate-polyacrylamide gel electrophoresis using 8% or 10% gels and the Mini-Protein II apparatus (Bio-Rad, CA, United States). Protein bands were electroblotted onto the polyvinylidene difluoride (PVDF ...
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bioRxiv - Plant Biology 2021Quote: ... Proteins were transferred on a PVDF membrane according to the manufacturer’s protocol (Trans-Blot® Turbo™ Rapid Transfer System, BIORAD, Les-Ulis, France) using a small protein transfer program ...
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bioRxiv - Cancer Biology 2021Quote: ... 20 to 30 μg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-rad) ...
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bioRxiv - Immunology 2021Quote: We mixed 500 μg of extracted cell lysates and 100 μL of patients’ sera with 100 μL of SureBeads™ Protein G Magnetic Beads (Bio-Rad, Hercules, CA) and added phosphate-buffered saline containing 0.2% of Tween 20 (PBS-T ...
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bioRxiv - Immunology 2021Quote: ... 20 to 30 µg of lysate protein was subjected to electrophoresis on a 4–15% gradient mini-Protean TGX gel (Bio-Rad, Hercules, CA, USA). It was then transferred to a membrane using a semidry method with a Trans-Blot Turbo Transfer System (Bio-Rad) ...
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bioRxiv - Cell Biology 2021Quote: ... Protein extracts (50 µg of protein) in β-mercaptoethanol containing SDS sample buffer were separated in 4% to 12% gradient SDS-polyacrylamide gels (Bio-Rad #456-8094) and transferred to nitrocellulose membranes (Bio-Rad #170-4271 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5 μl of serial dilutions of standard protein and samples were mixed with 250 μl of Bradford reagent (500-0205, BIO-RAD Quick Start™). Samples were incubated 10 minutes at room temperature and measured at OD595 using an ELISA plate reader (TECAN Infinite M200 Pro) ...
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bioRxiv - Biochemistry 2022Quote: Protein overexpression and purification were analysed via 10% SDS-PAGE and imaged via ChemiDoc™ MP Imaging System (Bio-Rad; Fig. S1 and S4). Immunoblotting was performed to confirm purification results with primary anti-6xHis polyclonal mouse antibody (Beyotime® ...
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bioRxiv - Cell Biology 2022Quote: ... The radio-labelled proteins were then separated with SDS-PAGE on a 4-12% Criterion XT Bis-Tris gels (Bio-Rad, Hercules, CA, USA) in MES buffer (Bio-Rad) ...